Cultive of the marine microalgae Chaetoceros calcitrans (Bacillariophyceae) using differents brands of artificial marine salt water

Abstract

The use of microalgae as live food to a wide variety of organisms is one of the most important aspects in aquaculture. Several commercial formulations have been available in the marine aquarium market in order to prepare artificial sea water (ASW). The present study compared microalgae Chaetoceros calcitrans performance cultured using different ASW in relation to natural sea water(NSW). It was carried out using Red Sea, Coralife, Oceanic, three different ASW brands and NSW as control. No aerated cultures were grew in 400 mL with Conwy culture medium with five replicates each one under defined conditions. All cultures began with an algal inoculum of 208,000 cells/mL. A 5-mL aliquot was removed daily from each culture for cells counts. Data obtained using polynomial regression test demonstrated that all ASW brands reached higher algal density rates than the one with NSW, though the three ASW brands were significantly heterogeneous. C. calcitrans raised with Oceanic and Red Sea brands showed similar growth rates and both were higher than Coralife brand. The results suggest that all three ASW brands studied can be used in the culture of this microalgae species.