Induction of triploidy in Rhamdia quelen (Pisces, Pimelodidae)

Authors

  • Pedro Vozzi Universidad Nacional de Misiones, Facultad de Ciencias Exactas, Quí­­micas y Naturales Dirección/)./Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Genética Dirección
  • Sebastián Sánchez Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias, INICNE Dirección
  • Enrique Permingeat Universidad Nacional de Misiones, Facultad de Ciencias Exactas, Quí­­micas y Naturales Dirección

Keywords:

triploidy, nucleolus, heat shocks, catfish, Rhamdia quelen

Abstract

 

The triploidy induction technique in Rhamdia quelen was standardized by the application of heat shocks with different durations and in different moments post-fecundation. The survival to the heat shock was measured in the moment of larvae eclosion. In order to identify the triploid fishes, the rate of ploidy was calculated by recounting the maximum number of nucleolus in interphase nuclei. The best method to induce triploidy in Rhamdia quelen was determined based on the values of the variables analyzed in this paper. The variable that produced the greatest rate of survival to the heat shock was also indicated - temperature of the shock: 36 ± 1°C; duration of the temperature shock: 5 minutes; moment of the shock temperature: 5 minutes post-fecundation, with triploidy and survival rates of 74.8% and 33.4%, respectively.

Downloads

Published

2018-07-02

Issue

Vol. 29 No. 1 (2003): BOLETIM DO INSTITUTO DE PESCA

Section

Scientific Article

Most read articles by the same author(s)