Liver response to dietary supplementation in bullfrog under stress condition

Abstract

The hepatoprotective effect of dietary supplementation with the probiotic (Bacillus subtilis) and with beta-glucan on bullfrog in stress of high stocking density were evaluated . Animals were assigned in treatments: T1: 100 animals m^-2 (control), T2: 236 animals m^-2 and T3: 236 animals m^-2, supplemented with probiotic; T4: 236 animals m^-2, supplemented with beta-glucan. Survival, weight gain (WG) and the condition of liver biochemistry and histopathology were evaluated. There was no statistical difference in survival between treatments (P>0.05), but were observed significant differences for WG at 30 days, between unsupplemented treatments and supplemented treatments (P<0.001). Biochemical analysis showed conditions very near among animals of control treatment and animals supplemented with beta-glucan. Histopathological analysis revealed lesions and cytoplasmic rarefaction in 100% of the samples, suggesting protein deficiency. The beta-glucan reduced the effects of stress in bullfrog and has shown hepatoprotective effect, but the probiotic Bacillus subtilis was did not demonstrated the same effect.