PROBIOTIC Bacillus subtilis AND Lactobacillus plantarum IN DIET OF NILE TILAPIA

Authors

  • Mateus Cardoso Guimarães Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios -  APTA, Programa de Pós-graduação do Instituto de Pesca
  • Danielle de Carla Dias Fundação de Amparo Á  Pesquisa do Estado de São Paulo -  FAPESP, Programa Jovem Pesquisador / Governo do Estado de São Paulo, Secretaria da Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios -  APTA, Instituto de Pesca http://orcid.org/0000-0002-8375-5948 (unauthenticated)
  • Felipe von Atzingen Pereira de Araujo Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios -  APTA, Programa de Pós-graduação do Instituto de Pesca
  • Carlos Massatoshi Ishikawa Governo do Estado de São Paulo, Secretaria da Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios -  APTA, Instituto de Pesca
  • Leonardo Tachibana Governo do Estado de São Paulo, Secretaria da Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios -  APTA, Instituto de Pesca

DOI:

https://doi.org/10.20950/1678-2305.2019.45.1.252

Keywords:

microbiologia, nutrição, desempenho zootécnico, expressão gênica, pós-larva

Abstract

The aim of this study was to evaluate the effects of a probiotic, composed of Bacillus subtilis and Lactobacillus plantarum in Nile tilapia fry during the sex reversal phase under stress conditions caused by high stocking density. This experiment was conducted in the Fisheries Institute of São Paulo. The experiment design was completely randomized with four treatments: 1) probiotic added feed, 2) probiotic added to water, 3) probiotic added to feed and water and 4) control diet; with three replicates. The variables analyzed were: final weight, total length, specific growth rate, survival, intestinal microbiology and gene expression of TNF-α and HSP-70. The results of the zootechnical performance of growth and gene expression did not show significant differences between treatments in the parameters evaluated (P>0.05). In the intestinal tract of fry raised in water with added probiotic, log10 CFU (7.72 ± 0.51) count of Bacillus spp. was higher than with other treatments which themselves did not differ significantly. It was concluded that the addition of a probiotic of Bacillus subtilis and Lactobacillus plantarum in the feed or water of Nile tilapia fry during the sex reversal phase did not affect the zootechnical performance of growth or expression the genes studied, but modified the intestinal microbiota.

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Published

2019-01-22

Issue

Vol. 45 No. 1 (2019): BOLETIM DO INSTITUTO DE PESCA

Section

Scientific Article

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