Prebiotic, probiotic and marine algae suplementation in juvenile tilapia diet

Maria José T. Ranzani-Paiva; Danielle de Carla Dias; Raissa Bertoncello Cavalcante; Guilherme Telli; Eliana Oshiro; Carlos Massatoshi Ishikawa; Maria Letizia Petesse; Miguel Frederico Fernandez-Alarcon; Mariene Miyoko Natori; Miguel Angel Moriñigo; Silvana Tapia; Said Ben Hamed; Leonardo Tachibana

doi:10.20950/1678-2305/bip.2024.50.e819

Authors

Maria José T. Ranzani-Paiva Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0001-8279-047X
Danielle de Carla Dias Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0002-8375-5948
Raissa Bertoncello Cavalcante Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil.
Guilherme Telli Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil.
Eliana Oshiro Universidade Estadual Paulista – Centro de Aquicultura – Jaboticabal (SP) – Brazil.
Carlos Massatoshi Ishikawa Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0002-0730-0706
Maria Letizia Petesse Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0001-6094-671X
Miguel Frederico Fernandez-Alarcon Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0001-7206-2209
Mariene Miyoko Natori Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0003-0471-0096
Miguel Angel Moriñigo Universidad de Málaga – Departamento de Microbiologia –Málaga – Spain. https://orcid.org/0000-0003-1452-4844
Silvana Tapia Universidad de Málaga – Departamento de Microbiologia –Málaga – Spain. https://orcid.org/0000-0002-3656-5009
Said Ben Hamed Universidad de Málaga – Departamento de Microbiologia –Málaga – Spain. https://orcid.org/0000-0001-6154-4667
Leonardo Tachibana Instituto de Pesca – Centro de Pesquisa em Aquicultura –São Paulo (SP) – Brazil. https://orcid.org/0000-0002-5847-8723

DOI:

https://doi.org/10.20950/1678-2305/bip.2024.50.e819

Keywords:

Immunostimulants, Symbiotics, Nutrition, Hematology, Aeromonas hydrophila, Aquaculture

Abstract

This study evaluated the probiotic mannan oligosaccharide (MOS), and marine algae for Nile tilapia (Oreochromis niloticus) feeding. The parameters analyzed were fish-growth rate, hematological and immunological parameters, and intestinal microbiota. Also, fish were submitted to experimental infection challenge with Aeromonas hydrophila to evaluate the immune response. Nile tilapia juveniles (weight 8.86 ± 3.22 g) were used in the six treatments with four replicates, for 63 days. The treatments were: control basal diet; PAS-TR: basal diet plus 0.04 g·kg^-1 of the probiotic (Bacillus cereus and Bacillus subtilis); MOS: basal diet plus 4 g·kg^-1 of the prebiotic mannooligosaccharide, 4 g·kg^-1of the prebiotic Kappaphycus alvarezii. Two more diets were formulated by a combination of PAS-TR + MOS (4 +4 g·kg^-1), and PAS-TR + KAP (4 +4 g·kg^-1). For the challenge experiment, the fish were fed for 21 days, infected via intraperitoneal injection with A. hydrophila (1 x 106 UFC·mL^-1), and the mortality rate was registered for 15 days post-infection. Results indicated the capacity of probiotic to remain in the gut for 63 days, and it was inhibited neither by autochthonous microbiota nor by prebiotics used. The feed additives tested for Nile tilapia did not cause a beneficial or adverse effect on growth or hematological variables. However, these treatments protected the fish from A. hydrophyla infection, proved by higher survival rate, and relative protection levels. We concluded that probiotic PAS-TR and prebiotics MOS and KAP, combined or not as symbiotics, may promote immune protection and reduce the mortality rate of A. hydrophyla infection.

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