Oxytetracycline for chemical marking of piava juveniles Leporinus obtusidens: determining concentration and time of treatment
Keywords:
scale, mass-marking, calcified structuresAbstract
The aim of this study was to define the concentrations and immersion periods for the chemical marking of piava juveniles Leporinus obtusidens using oxytetracycline (OTC). Twenty piava juveniles were used per treatment and submitted to six concentrations of OTC (50, 100, 150, 300, 500, 800 mg OTC L-1) and three immersion periods (6, 12, 24 h) and one treatment as control. Scales of three fishes per treatment were evaluated under a confocal microscope with UV light. The mean values of temperature, pH and dissolved oxygen were kept at 27.4°C, 7.2, and 7.4 mg L-1, respectively. No mortality was observed in the treatments. The presence of a chemical mark was only observed in the treatment 100 mg L-1 24 h-1, however it was of low intensity and discontinuous. Treatments with immersion periods of 12 and 24 h produced visible marks in 300 mg L-1, and clear bright and continuous marks in 500 mg L-1. Treatments of 800 mg L-1, for all immersion periods, produced clear bright and continuous marks.
References
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oxytetracycline. North American Journal of Fisheries Management, 24: 262-269.
BLANKENSHIP, H.L. e LEBER, K.M. 1995 A responsible approach to marine stock
enhancement. American Fisheries Society Symposium, 15: 167-175.
BROOKS, R.C.; HEIDINGER, R.C.; KOHLER, C.C.1994 Mass-marking otoliths of larval and juvenile walleyes by immersion in oxytetracycline, calcein, or calcein blue. North
American Journal of Fisheries Management, 14: 143-150.
BUTCHER, A.; MAYER, D.; WILLETT, D.;JOHNSTON, M.; SMALLWOOD, D. 2003 Scale
pattern analysis is preferable to OTC marking of otoliths for differentiating between stocked and wild dusky flathead, Platycephalus fuscus, and sand whiting, Sillago ciliate. Fisheries Management and Ecology, 10: 163-172.
CRUMPTON, R.L.; HENNE, J.P.; WARE, K.M. 2012 Marking otoliths and fin spines of juvenile shortnose sturgeon with oxytetracycline and the effects of water temperature during treatment. North American Journal of Fisheries Management,
32: 523í 527.
FIELDER, D.G. 2002 Methodology for immersion marking walleye fry and fingerlings in oxytetracycline hydrochloride and its detection with fluorescence microscopy. Michigan Department of Natural Resources/Fisheries Technical Report.23p.
IGLESIAS, J. e RODRÍGUES-OJEA. G. 1997 The use of alizarin complexone for immersion marking of the otoliths of embryos and larvae of the turbot, Scophthalmus maximus (L.): dosage and treatment time. Fisheries Management and Ecology,4: 405-417.
JENKINS, W.E.; DENSON, M.R.; BRIDGHAM, C.B.;COLLINS, M.R.; SMITH, T.I.J. 2002 Retention of oxytetracycline-induced marks on sagittae of red drum. North American Journal of Fisheries Management, 22: 590í 594.
LEAL, M.E.; BARBOSA, A.S.; SCHULZ, U.H. 2012 Uso de Implante Visual Fluorescente de Elastômero (VIFE) na marcação de pequenos peixes de água doce. Biotemas, 25(3): 311-315.
LOCHET, A.; JATTEAU, P.; GESSNER, J. 2011 Detection of chemical marks for stocking
purposes in sturgeon species. Journal of Applied Ichthyology, 27: 444-449.
MEERBEEK, J.R. e BETTOLI, P.W. 2005 MassMarking Rainbow Trout and Brown Trout
Fingerlings with Oxytetracycline. Journal of Freshwater Ecology, 20(3): 555-559.
MORALES-NIN, B.; GRAU, A.; PÉREZ-MAYOL, S.;PASTOR, E.; PALMER, M. 2011 Oxytetracycline hydrochloride vital labelling revisited: the case of Dicentrarchus labras and Diplodus puntazzo.Journal of Fish Biology, 78: 762-782.
REIS, R.E.; KULLANDER, S.O.; FERRARIS-JR, C.J. 2003 Check list of the freshwater fishes of South and Central America. Porto Alegre:EDIPUCRS. 742p.
REYNALTE-TATAJE, D.A. e ZANIBONI-FILHO, E. 2010 Cultivo do gênero Leporinus. In:
BALDISSEROTTO, B. e GOMES, L.C. (org.) Espécies nativas para a piscicultura no Brasil. Santa Maria, Editora UFSM. p.81-103.
TAYLOR, M.D.; FIELDER, D.S.; SUTHERS, I.M. 2005 Batch marking of otoliths and fin spines to assess the stock enhancement of Argyrosomus japonicas. Journal of Fish Biology, 66: 1149-1162.
WARTENBERG, R.; BOOTH, A.J.; WEYL, O.L.F.2011 A comparison of three techniques for fluorocrhome marking of juvenile Clarias gariepinus otoliths. African Zoology, 46(1): 72-77.
XU, D. e ROGERS, W.A. 1994 Oxytetracycline residue in striped bass muscle. Journal of Aquatic Animal Health, 6: 349-354.
ZANIBONI-FILHO, E. 2004 Piscicultura das espécies nativas de água doce. In: POLI, C.R.; POLI,A.T.B.; ANDREATTA, E.; BELTRAME, E. (org.)Aquicultura: Experiências brasileiras. Florianópolis,Ed. UFSC. p.337-368.
ZANIBONI-FILHO, E. e SCHULZ, U.H. 2003 Migratory fishes of the Uruguay River. In:
CAROLSFELD, J.; HARVEY, B.; BAER, A.; ROSS, C. (eds.) Migratory fishes of the South
America: biology, social importance and conservation status. Canada, IDRC/World Bank/World Fisheries Trust. p.135-168.
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Published
2018-11-16
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