Tetraploidy in Rhamdia quelen (Quoy and Gaimard 1824) by a dual thermal shock (heat and cold)
DOI:
https://doi.org/10.20950/1678-2305.2017v43n2p257Keywords:
jundiá, tetraploidy, thermal shock, poliploidyAbstract
The efficiency of double thermal shock to induce tetraploidy in Rhamdia quelen (jundiá) at different post-fertilization periods was assessed. Each test was performed with 20 mL of eggs (10, 15, 20, 25, 30 and 35 mpf í minutes post-fertilization) exposed to hot thermal shock (39 ± 0.2°C) for 3 min, followed by cold thermal shock (1.0 ± 0.1°C) for 30 min. A control group was also employed. Fertilization and hatching rates were evaluated 12 and 30 hours post-fertilization (hpf) respectively. After 60 hpf, twenty-five larvae from each experimental unit were fixed to verify the ploidy by flow cytometry. Control fertilization rate was 87.83% and reached 23.4%, 28.5%, 30.4%, 20.0%, 30.3% and 36.7%, respectively for 10, 15, 20, 25, 30 and 35 mpf. Tetraploids were detected only at 15 mpf and 20 mpf. This is the first research of tetraploidy in jundiá obtained by hot and cold shocks, making possible the improvement of this technique.
References
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ARAI, K. 1991 Genetic improvement of aquaculture finfish species by chromosome manipulation
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ÁVILA, M.C. 2004 Indução í tetraploidia em tilápia nilótica Oreochromis niloticus, utilizando-se choque térmico. Jaboticabal: Mauricio Carrillo Ávila. 59p.
BALDISSEROTTO, B.; RADíÅ“NZ-NETO, J. 2004 Criação de jundiá. Santa Maria: Universidade
Federal de Santa Maria. 232p.
BLANC, J.M.; CHOURROUT, D.; KRIEG, F. 1987 Evaluation of juvenile rainbow trout survival and growth in half-sib families from diploid and tetraploid sires. Aquaculture, 65(3-4): 215í 220.
CESAR, M.P.; MURGAS, L.D.S.; ARAíÅ¡JO, R.V.; DRUMMOND, C.D. 2004 Método para obtenção
de população monosexo na piscicultura. Lavras:Universidade Federal de Lavras, Boletim Agropecuário, 69, 27p.
CHERFAS, N.B.; GOMELSKY, B.I.; EMELYANOVA,O.V.; RECOUBRATSKY, A.V. 1994 Induced
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DITER A.; QUILLET E.; CHOURROUT D. 1993 Suppression of first egg mitosis induced by heat
shocks in the rainbow trout. Journal Fish Biology, 42(5): 777í 86.
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FLAJSHANS M.; LINHART O.; KVASNICKA P. 1993 Genetic studies of tench (Tinca tinca L.): induced
triploidy and tetraploidy and first performance data. Aquaculture, 113(4): 301í 312.
FRACALOSSI, D.M.; MEYER, G.; SANTAMARIA, F.M.; WEINGARTNER, M.; ZANIBONI-FILHO,E. 2004 Desempenho do jundiá, Rhamdia quelen,e do dourado, Salminus brasiliensis, em viveiros de terra na região sul do Brasil. Acta Scientiarum Animal Sciences, 26(3): 43-49.
GARCIA, S.; AMARAL-JUNIOR, H.; SOUTO, L.I.M.;WARMILING, P.F.; BERNARDES JUNIOR,J.J. 2013 Cultivo mono sexo de jundiá Rhamdia quelen. In: S. Garcia & H. Amaral Junior (Eds.). O Jundiá Rhamdia quelen: Relatos de avanços no cultivo do peixe de água doce nativo mais promissor da
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GUI, J.; SUN, J.; LIAN, G.S.; HUANG, W.; JIANG, Y. 1991 Studies on genome manipulation in fish:
II. Tetraploidy induced by hydrostatic pressure treatment and a combination of hydrostatic
pressure and cold treatments in transparent colored crucian carp. Acta Hydrobiolica Sinica,
15(4): 333í 342.
HONG, Y. 1990 Tetraploidy induced by heat shock in bighead carp, Aristichthys nobilis. Acta Zoologica Sinica, 36(1): 70í 75.
HORSTGEN-SCHWARK, G. 1993 Initiation of tetraploid breeding line development in rainbow trout, Oncorhynchus mykiss (Walbaum).Aquaculture Fish Management, 24(5): 641í 652.
HUERGO, G.M.; ZANIBONI-FILHO, E. 2006 Triploidy Induction in Jundiá (Rhamdia quelen,Quoy & Gaimard 1824) through hydrostatic pressure shock. Journal of Applied Aquaculture,18(4): 45-57.
KOBAYASHI, H. 1971 A cytological study on gynogenesis on triploid ginbuna (Carassius auratus langsdorfii). Acta Hydrobiolica Sinica,80(4), 316í 322.
KUSUNOKI, T.; ARAI, K.; SUZUKI, R. 1994 Production of viable gynogens without chromosome duplication in thespinous loach Cobitis biwae. Aquaculture, 119(1): 11í 23
LAMATSCH, D.K.; STEINLEIN, C.; SCHMID M.; SCHARTL, M. 2000 Non invasive determination
of genome size and ploidy level in fishes by flow cytometry: detection of triploid Poecilia formosa.
Cytometry, 39(2): 91-95.
LE COMBER, S.C.; SMITH, C. 2004 Polyploidy in fishes: patterns and processes. Biological Journal
of the Linnean Society, 82(4): 431-442.
LOZANO, R.; RUIZ, C.; RUIZ, M. 1987 Manipulación cromosómica en organismos acuáticos. p. 215-
246. In: Espinosa De Los Monteros, J. & Labarta,U. (Eds.). Genética en acuicultura. Madrid: Caycit.
274 p.
MALISON, J.A.; PROCARIONE, L.S.; HELD, J.A.,KAYES, T.B.; AMUNDSON, C.H. 1993 The influence of triploidy and heat and hydrostatic pressure shocks on the growth and reproductive development of juvenile yellow perch (Perca flavescens). Aquaculture, 116(2-3): 121-133.
MELO, D.C.; OLIVEIRA, D.A.A.; SOUSA, A.B.;CARVALHO, D.C.; SEERIG, A. S.; CREPALDI,D.V.; TEIXEIRA, E.A.; RIBEIRO, L.P.; FARIA, P.M.C. 2006 Manipulação cromossômica:aplicações práticas na aquacultura. Revista Brasileira de Reprodução Animal, 30(2): 105-112.
NAM, Y.K.; CHOI, G.C.; KIM D.S. 1999 Blocking of the first cleavage in mud loach (Misgurnus
mizolepis). Aquaculture, 12(3): 167í 73.
NAM, Y.K.; CHO, G.C.; KIM, D.S. 2004 An efficient method for blocking the 1st mitotic cleavage of
fish zygote using combined thermal treatment, exemplified by mud loach (Misgurnus mizolepis).
Theriogenology, 61(5): 933-945.
PANDEY, N.; LAKRA, W.S. 1997 Evidence of Female Heterogamety, B-Chromosome and Natural
Tetraploidy in the Asian catfish, Clarias batraclus,used in Aquaculture. Aquaculture, 149(1-2): 31-
37.
PANDIAN, T.J.; KOTEESWARAN, R. 1998 Ploidyinduction and sex control in fish. Hydrobiologia,
384(1): 167í 243
PIFERRER, F.; BEAUMONT, A.; FALGUIERE, J.C.2009 Polyploid fish and shellfish: Production,
biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture, 293(3-4): 125-156.
PRESTON A.C.; TAYLOR J.F.; CRAIG B.,BOZZOLLA P.; PENMA N.D.J.; MIGAUD H. 2013 Optimisation of triploidy induction in brown trout (Salmo trutta L.). Aquaculture, 415-416: 160í 166.
RIZZO, E.; GODINHO, H.P.; SATO, Y. 2003 Shortterm storage of oocytes from the neotropical teleost fish Prochilodus marggravii. Theriogenology,60(6): 1059-1070.
RODRIGUES-GALDINO, A.M.; MAIOLINO C.V.;FORGATI M., DONATTI L.; MIKOS J.D.;CARNEIRO P.C. F.; SANT’ANNA F.R. 2009 Development of the neotropical catfish Rhamdia quelen (Siluriformes, Heptapteridae) incubated in different temperature regimes. Zygote, 18(2):131í 144.
SILVA, F.S.D.; MOREIRA, R.G.; OROZCO-ZAPATA,C.R.; HILSDORF, A.W.S. 2007 Triploidy induction by cold shock in the South American catfish, Rhamdia quelen (Siluriformes) (Quoy &Gaimard, 1824). Aquaculture, 272(Supplement 1): 110-114.
TABATA, Y.A.; RIGOLINO, M.G.; TSUKAMOTO,R.Y. 1999 Production of all female triploid rainbow-trout, Oncorhynchus mykiss (Pisces,Salmonidae). III í Growth up to first sexual maturation. Boletim do Instituto de Pesca,25(único): 67-76.
TEBALDI, P.C.; AMARAL-JUNIOR, H. 2009 Production of tetraploid nile tilapia (Oreochromis
niloticus) by the application of thermal shock.Revista electrónica de Veterinaria, 10. Disponivel
em: <http://www.veterinaria.org/revistas/redvet/n101009/100908.pdf> Acesso em: 14 ago. 2014.
THORGAARD, G.; JAZWIN, M.E.; STIER, A.R. 1981 Polyploidy induced by heat shock in rainbow
trout. Transactions of the American Fisheries Society, 110(4), 546-550.
TURRA, E.M.; OLIVEIRA, D.A.A.; VALENTE, B.D.2012 Estimation of genetic parameters for body
weights of Nile tilapia Oreochromis niloticus using random regression models. Aquaculture,
354-355: 31-37.
VOZZI, P.A.; SANCHEZ, S; PERMINGEAT, E.D.2003 Inducción de triploidia em Rhamdia quelen.
Boletim do Instituto de Pesca, 29(1):87-94.
WEBER G.M.; HOSTUTTLER M.A. 2012 Factors affecting the first cleavage interval and effects of
parental generation on tetraploid production in rainbow trout (Oncorhynchus mykiss) Aquaculture,344-349: 231í 238.
WIRTZ, S.; STEINMANN P. 2006 Sperm characteristics in perch Perca fluviatilis. Journal of Fish Biology, 68(6): 1896-1902.
WOYNAROVICH, E.; HORVATH, L.A. 1983 A propagação artificial de peixes de águas tropicais:
Manual de extensão. Brasilia: FAO/CODEVASF/CNPq, Documento Técnico sobre Pesca 201.225p.
YAMAZAKI, F.; GOODIER, J. 1993 Cytogenetic effects of hydrostatic pressure treatment to
suppress the first cleavage of salmon embryos. Aquaculture, 110(1): 51í 59.
ZANIBONI-FILHO, E. 1992 Incubação, larvicultura e alevinagem do tambaqui (Colossoma macropomum Cuvier 1818). São Carlos. 202p. (Tese deDoutorado. Universidade Federal de São
Carlos).
ZAR, J.H. 2010 Biostatistical analysis. New Jersey: Prentice Hall. 944p.
ALMEIDA, R.B.C. 2007 Astyanax altiparanae (Pisces, Characiformes) como modelo biológico
de espécie de peixe para exploração zootécnica e biomanipulação. Botucatu. 119f. (Tese de
Doutorado. Universidade Estadual Paulista í UNESP).
AMARAL-JUNIOR, H. 2007 Manual de reprodução de peixes de água doce com cultivo comercial na
região Sul do Brasil. Florianópolis: Epagri,BoletimTécnico 136, 53p.
ARAI, K. 1991 Genetic improvement of aquaculture finfish species by chromosome manipulation
techniques in Japan. Aquaculture, 197(1-4): 205-228.
ÁVILA, M.C. 2004 Indução í tetraploidia em tilápia nilótica Oreochromis niloticus, utilizando-se choque térmico. Jaboticabal: Mauricio Carrillo Ávila. 59p.
BALDISSEROTTO, B.; RADíÅ“NZ-NETO, J. 2004 Criação de jundiá. Santa Maria: Universidade
Federal de Santa Maria. 232p.
BLANC, J.M.; CHOURROUT, D.; KRIEG, F. 1987 Evaluation of juvenile rainbow trout survival and growth in half-sib families from diploid and tetraploid sires. Aquaculture, 65(3-4): 215í 220.
CESAR, M.P.; MURGAS, L.D.S.; ARAíÅ¡JO, R.V.; DRUMMOND, C.D. 2004 Método para obtenção
de população monosexo na piscicultura. Lavras:Universidade Federal de Lavras, Boletim Agropecuário, 69, 27p.
CHERFAS, N.B.; GOMELSKY, B.I.; EMELYANOVA,O.V.; RECOUBRATSKY, A.V. 1994 Induced
diploid gynogenesis and polyploidy in the crucian carp Carassius auratus gibelio (Bloch)
x common carp Cyprinus carpio, L. Hybrids.Aquaculture Fish Management, 25(9): 943í 954.
CHOURROUT, D. 1987 Genetic manipulations in fish: review of methods, In: K. Tiews (Ed.).Selection, Hybridization and Genetic Engineering in Aquaculture, Schriften der BundesforschungsanstaltfürFischerei, Heenemann, Berlin p.111-126.
CHOURROUT, D.; CHECASSUS, B.; KRIEG, F.;HAPPE, A.; BURGER, G.; RENARD, P. 1986 Production of second generation triploid and tetraploid rainbow trout by mating tetraploid males and diploid females potential of tetraploid fish. Theriogenology Applicade Genetic, 72(2):193í 206.
DITER A.; QUILLET E.; CHOURROUT D. 1993 Suppression of first egg mitosis induced by heat
shocks in the rainbow trout. Journal Fish Biology, 42(5): 777í 86.
FANJUL; L.C.; TORO, M.A. 1991 Mejora genética depeces y moluscos. Madrid: Mundi Prensa. 110p.
FLAJSHANS M.; LINHART O.; KVASNICKA P. 1993 Genetic studies of tench (Tinca tinca L.): induced
triploidy and tetraploidy and first performance data. Aquaculture, 113(4): 301í 312.
FRACALOSSI, D.M.; MEYER, G.; SANTAMARIA, F.M.; WEINGARTNER, M.; ZANIBONI-FILHO,E. 2004 Desempenho do jundiá, Rhamdia quelen,e do dourado, Salminus brasiliensis, em viveiros de terra na região sul do Brasil. Acta Scientiarum Animal Sciences, 26(3): 43-49.
GARCIA, S.; AMARAL-JUNIOR, H.; SOUTO, L.I.M.;WARMILING, P.F.; BERNARDES JUNIOR,J.J. 2013 Cultivo mono sexo de jundiá Rhamdia quelen. In: S. Garcia & H. Amaral Junior (Eds.). O Jundiá Rhamdia quelen: Relatos de avanços no cultivo do peixe de água doce nativo mais promissor da
região sul do Brasil. Camboriú, EPAGRI, Gráfica Delta.p.32-41.
GOMES, L.C.; GOLOMBIESKI, J.I.; GOMES, A.R.C. 2000 Biologia do jundiá Rhamdia quelen (Teleostei,Pimelodidae). Ciência Rural, 30(1):179-185.
GUI, J.; SUN, J.; LIAN, G.S.; HUANG, W.; JIANG, Y. 1991 Studies on genome manipulation in fish:
II. Tetraploidy induced by hydrostatic pressure treatment and a combination of hydrostatic
pressure and cold treatments in transparent colored crucian carp. Acta Hydrobiolica Sinica,
15(4): 333í 342.
HONG, Y. 1990 Tetraploidy induced by heat shock in bighead carp, Aristichthys nobilis. Acta Zoologica Sinica, 36(1): 70í 75.
HORSTGEN-SCHWARK, G. 1993 Initiation of tetraploid breeding line development in rainbow trout, Oncorhynchus mykiss (Walbaum).Aquaculture Fish Management, 24(5): 641í 652.
HUERGO, G.M.; ZANIBONI-FILHO, E. 2006 Triploidy Induction in Jundiá (Rhamdia quelen,Quoy & Gaimard 1824) through hydrostatic pressure shock. Journal of Applied Aquaculture,18(4): 45-57.
KOBAYASHI, H. 1971 A cytological study on gynogenesis on triploid ginbuna (Carassius auratus langsdorfii). Acta Hydrobiolica Sinica,80(4), 316í 322.
KUSUNOKI, T.; ARAI, K.; SUZUKI, R. 1994 Production of viable gynogens without chromosome duplication in thespinous loach Cobitis biwae. Aquaculture, 119(1): 11í 23
LAMATSCH, D.K.; STEINLEIN, C.; SCHMID M.; SCHARTL, M. 2000 Non invasive determination
of genome size and ploidy level in fishes by flow cytometry: detection of triploid Poecilia formosa.
Cytometry, 39(2): 91-95.
LE COMBER, S.C.; SMITH, C. 2004 Polyploidy in fishes: patterns and processes. Biological Journal
of the Linnean Society, 82(4): 431-442.
LOZANO, R.; RUIZ, C.; RUIZ, M. 1987 Manipulación cromosómica en organismos acuáticos. p. 215-
246. In: Espinosa De Los Monteros, J. & Labarta,U. (Eds.). Genética en acuicultura. Madrid: Caycit.
274 p.
MALISON, J.A.; PROCARIONE, L.S.; HELD, J.A.,KAYES, T.B.; AMUNDSON, C.H. 1993 The influence of triploidy and heat and hydrostatic pressure shocks on the growth and reproductive development of juvenile yellow perch (Perca flavescens). Aquaculture, 116(2-3): 121-133.
MELO, D.C.; OLIVEIRA, D.A.A.; SOUSA, A.B.;CARVALHO, D.C.; SEERIG, A. S.; CREPALDI,D.V.; TEIXEIRA, E.A.; RIBEIRO, L.P.; FARIA, P.M.C. 2006 Manipulação cromossômica:aplicações práticas na aquacultura. Revista Brasileira de Reprodução Animal, 30(2): 105-112.
NAM, Y.K.; CHOI, G.C.; KIM D.S. 1999 Blocking of the first cleavage in mud loach (Misgurnus
mizolepis). Aquaculture, 12(3): 167í 73.
NAM, Y.K.; CHO, G.C.; KIM, D.S. 2004 An efficient method for blocking the 1st mitotic cleavage of
fish zygote using combined thermal treatment, exemplified by mud loach (Misgurnus mizolepis).
Theriogenology, 61(5): 933-945.
PANDEY, N.; LAKRA, W.S. 1997 Evidence of Female Heterogamety, B-Chromosome and Natural
Tetraploidy in the Asian catfish, Clarias batraclus,used in Aquaculture. Aquaculture, 149(1-2): 31-
37.
PANDIAN, T.J.; KOTEESWARAN, R. 1998 Ploidyinduction and sex control in fish. Hydrobiologia,
384(1): 167í 243
PIFERRER, F.; BEAUMONT, A.; FALGUIERE, J.C.2009 Polyploid fish and shellfish: Production,
biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture, 293(3-4): 125-156.
PRESTON A.C.; TAYLOR J.F.; CRAIG B.,BOZZOLLA P.; PENMA N.D.J.; MIGAUD H. 2013 Optimisation of triploidy induction in brown trout (Salmo trutta L.). Aquaculture, 415-416: 160í 166.
RIZZO, E.; GODINHO, H.P.; SATO, Y. 2003 Shortterm storage of oocytes from the neotropical teleost fish Prochilodus marggravii. Theriogenology,60(6): 1059-1070.
RODRIGUES-GALDINO, A.M.; MAIOLINO C.V.;FORGATI M., DONATTI L.; MIKOS J.D.;CARNEIRO P.C. F.; SANT’ANNA F.R. 2009 Development of the neotropical catfish Rhamdia quelen (Siluriformes, Heptapteridae) incubated in different temperature regimes. Zygote, 18(2):131í 144.
SILVA, F.S.D.; MOREIRA, R.G.; OROZCO-ZAPATA,C.R.; HILSDORF, A.W.S. 2007 Triploidy induction by cold shock in the South American catfish, Rhamdia quelen (Siluriformes) (Quoy &Gaimard, 1824). Aquaculture, 272(Supplement 1): 110-114.
TABATA, Y.A.; RIGOLINO, M.G.; TSUKAMOTO,R.Y. 1999 Production of all female triploid rainbow-trout, Oncorhynchus mykiss (Pisces,Salmonidae). III í Growth up to first sexual maturation. Boletim do Instituto de Pesca,25(único): 67-76.
TEBALDI, P.C.; AMARAL-JUNIOR, H. 2009 Production of tetraploid nile tilapia (Oreochromis
niloticus) by the application of thermal shock.Revista electrónica de Veterinaria, 10. Disponivel
em: <http://www.veterinaria.org/revistas/redvet/n101009/100908.pdf> Acesso em: 14 ago. 2014.
THORGAARD, G.; JAZWIN, M.E.; STIER, A.R. 1981 Polyploidy induced by heat shock in rainbow
trout. Transactions of the American Fisheries Society, 110(4), 546-550.
TURRA, E.M.; OLIVEIRA, D.A.A.; VALENTE, B.D.2012 Estimation of genetic parameters for body
weights of Nile tilapia Oreochromis niloticus using random regression models. Aquaculture,
354-355: 31-37.
VOZZI, P.A.; SANCHEZ, S; PERMINGEAT, E.D.2003 Inducción de triploidia em Rhamdia quelen.
Boletim do Instituto de Pesca, 29(1):87-94.
WEBER G.M.; HOSTUTTLER M.A. 2012 Factors affecting the first cleavage interval and effects of
parental generation on tetraploid production in rainbow trout (Oncorhynchus mykiss) Aquaculture,344-349: 231í 238.
WIRTZ, S.; STEINMANN P. 2006 Sperm characteristics in perch Perca fluviatilis. Journal of Fish Biology, 68(6): 1896-1902.
WOYNAROVICH, E.; HORVATH, L.A. 1983 A propagação artificial de peixes de águas tropicais:
Manual de extensão. Brasilia: FAO/CODEVASF/CNPq, Documento Técnico sobre Pesca 201.225p.
YAMAZAKI, F.; GOODIER, J. 1993 Cytogenetic effects of hydrostatic pressure treatment to
suppress the first cleavage of salmon embryos. Aquaculture, 110(1): 51í 59.
ZANIBONI-FILHO, E. 1992 Incubação, larvicultura e alevinagem do tambaqui (Colossoma macropomum Cuvier 1818). São Carlos. 202p. (Tese deDoutorado. Universidade Federal de São
Carlos).
ZAR, J.H. 2010 Biostatistical analysis. New Jersey: Prentice Hall. 944p.
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