Diagnostic locus for identification of silverside hybrids (odontesthes humensis and odontesthes bonariensis) in a natural population
DOI:
https://doi.org/10.20950/10.20950/1678-2305/bip.2022.48.e684Abstract
Hybridization is a natural phenomenon that occurs more often in fish than in other vertebrates. The use of nuclear and mitochondrial molecular markers provides valuable results in the detection of these events. The aim of this study was to investigate the occurrence of interspecific hybrids in natural populations of silverside. The samples of Odontesthes humensis, Odontesthes bonariensis, and individuals that were morphologically different from pure species were collected in the Mangueira lagoon, located in southern Brazil. Result: Six tetranucleotide microsatellite loci were synthesized and tested. The UFPEL_OH3 locus proved to be diagnostic for the detection of silverside hybrids, and it was possible to distinguish between pure and hybrid species. The mitochondrial marker gene cytb synthesized from conserved Odontesthes sequences in the GenBank genetic database showed no differences in the genetic sequence of the samples, needing further studies to confirm the hypothesis.
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Tavares, R.A.; Piedras, S.R.N.; Nunes, M.D.; Almeida, D.B.; Moreira, C.G.A.; Fernandes, J. M.; Freitas, S.F.; Moreira, H.L.M.; Pouey, J.L.O.F.; Dionello, N.J.L. 2014. Identificação de loci microssatélites com potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis). Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66(6): 1941-1945. https://doi.org/10.1590/1678-7444
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Wingert, J.M.; Ferrer, J.; Malabarba, L.R. 2017. Review of the Odontesthes perugiae species group from Río de La Plata drainage, with the description of a new species (Atherinomorpha: Atherinopsidae). Zootaxa, 4250(6): 501-528. https://doi.org/10.11646/zootaxa.4250.6.1
Allendorf, F.W. 2017. Genetics and the conservation of natural populations: allozymes to genomes. Molecular Ecology, 26(2): 420-430. https://doi.org/10.1111/mec.13948
Aziz, A.H.A.; Tarmizi, Z.; Ali, N.M.; Ariffin, N.A.; Abdullah, M.D.D.; Lian, W.L.; Giat, S.Y.; Aimi, T.N.; Jaafar, M.; Bolong, A.M.A.; Kasim, A.A.A.; Sheriff, S. 2015. Mitochondrial DNA diversity of terubok (tenualosa toli) from daro and mukah, sarawak inferred by partial Cytochrome b (Cytb). Journal of Fisheries and Aquatic Science, 10(2): 92-101. https://doi.org/10.3923 / jfas.2015.92.101
Bemvenuti, M.A. 2002. Diferenciação morfológica das espécies de peixes-rei, Odontesthes Evermann & Kendall (Osteichthyes, Atherinopsidae) no extremo sul do Brasil: morfometria multivariada. Revista brasileira de Zoologia, 19(1): 251-287. https://doi.org/10.1590/S0101-81752002000300013
Betancur-R, R.; Wiley, E.O.; Arratia, G.; Acero, A.; Bailly, N.; Miya, M.; Lecointre, G.; Ortí, G. 2017. Phylogenetic classification of bony fishes. BMC Evolutionary Biology, (17): 162. https://doi.org/10.1186/s12862-017-0958-3
Campanella, D.; Hughes, L.C.; Unmack, P.J.; Bloom, D.D.; PILLER, K. R.; ORTÍ, G. 2015. Multi-locus fossil-calibrated phylogeny of Atheriniformes (Teleostei, Ovalentaria). Molecular Phylogenetics and Evolution, 86(1): 8-23. https://doi.org/10.1016/j.ympev.2015.03.001
Eschmeyer, W.N. 2013. Catálogo de Peixes: Gêneros, Espécies, Referências. Academia de Ciências da Califórnia. [online] URL: <http://research.calacademy.org/research/ichthyology/catalog/fishcatmain.asp> Accessed: Dez. 20, 2019.
García, G.; Ríos, N.; Gutiérrez, V.; Varela, J.G.; Fernández, C.B.; Pardo, B.G.; Portela, P.M. 2014. Promiscuous speciation with gene flow in silverside fish genus Odontesthes (Atheriniformes, Atherinopsidae) from south western atlantic ocean basins. Plos One, 9(8): e104659. https://doi.org/10.1371/journal.pone.0104659
Hashimoto, D.H.; Mendonça, F.F.; Senhorini, J.A.; Bortolozzi, J.; Oliveira, C.; Foresti, F.; Porto-Foresti, F. 2010. Identification of hybrids between Neotropical fish Leporinus macrocephalus and Leporinus elongatus by PCR-RFLP and multiplex-PCR: tools for genetic monitoring in aquaculture. Aquaculture, 298(3-4): 346-349. http://doi.org/10.1016/j.aquaculture.2009.11.015
Hashimoto, D.H.; Mendonça, F.F.; Senhorini, J.A.; Oliveira, C.; Foresti, F.; Porto-Foresti, F. 2011. Molecular diagnostic methods for identifying Serrasalmid ï¬Âsh (Pacu, Pirapitinga, and Tambaqui) and their hybrids in the Brazilian aquaculture industry. Aquaculture, 321(1): 49-53. https://doi.org/10.1016/j.aquaculture.2011.08.018
Hashimoto, D.H.; Prado, F.D.; Senhorini, J.A.; Foresti, F.; Porto-Foresti, F. 2013. Detection of post-F1 ï¬Âsh hybrids in broodstock using molecular markers: approaches for genetic management in aquaculture. Aquaculture Research, 44(1): 876-884. http://doi.org/10.1111/j.1365-2109.2012.03092.x
Hasselman, D.J., Argo, E.E., McBride, M.C., Bentzen, P., Schultz, T.F., Perez-Umphrey, A.A. and Palkovacs, E.P. 2014. Human disturbance causes the formation of a hybrid swarm between two naturally sympatric fish species. Molecular Ecology, 23: 1137-1152. https://doi.org/10.1111/mec.12674
Hohenlohe, P.A.; Funk, W.C.; Rajora, O.P. 2021. Population genomics for wildlife conservation and management. Molecular Ecology, 30(1): 62-82. http://doi.org/10.1111/mec.15720
Hughes, L.C.; Cardoso, Y.P.; Sommer, L.A.; Cifuentes, R.; Cuello, M.; Somoza, G.M.; Gonzáles-Castro, M.; Malabarba, L.R.; Cussac, V.; Habit, E.M.; Betencur-R, R.; Ortí, G. 2020. Biogeography, habitat transitions and hybridization in a radiation of South American silverside fishes revealed by mitochondrial and genomic RAD data. Molecular Ecology, (29): 738-751. https://doi.org/10.1111/mec.15350
Kochzius, M. 2009. Trends in fishery genetics. In: BEAMISH, R.J.; ROTHSCHILD, B.J. (eds). The future of fisheries science in North America. Fish & Fisheries Series, 31: 453-493. http://doi.org/10.1007/978-1-4020-9210-7
Lopera-Barrero, N.M.L.; Povh, J.A.; Ribeiro, R.P.; Gomes, P.C.; Jacometo, C.B.; Lopes, T.S. 2008. Comparison of DNA extraction protocols of fish fin and larvae samples: modified salt (NaCl) extraction. Ciencia e investigación agrarian, 35(1): 65-74. http://doi.org/10.4067/S0718-16202008000100008
Porto-Foresti, F.; Hashimoto, D.T.; Prado, F.D.; Senhorini, J.A.; Foresti, F. 2013. Genetic markers for the identiï¬Âcation of hybrids among catï¬Âsh species of the family Pimelodidae. Journal of Applied Ichthyology, 29(3): 643-647. http://doi.org/10.1111/jai.12092
Prado, F.D.; Hashimoto, D.T.; Mendonca, F.F.; Senhorini, J.A.; Foresti, F.; Porto-Foresti, F. 2011. Molecular identiï¬Âcation of hybrids between Neotropical catï¬Âsh species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum. Aquaculture Research, 42(12):1890-1894. http://doi.org/10.1111/j.1365-2109.2010.02777.x
Rueda, E.C.; Mullaney, K.A.; Conte-Grand, C.; Habit, E.M.; Cussac, V.; Orti, G. 2016. Displacement of native Patagonian freshwater silverside populations (Odontesthes hatcheri, Atherinopsidae) by introgressive hybridization with introduced O. bonariensis. Biological Invasions, 19(3): 971-988. https://doi.org/10.1007/s10530-016-1295-y
Satoh, T.P.; Miya, M.; Mabuchi, K.; Nishida, M. 2016. Structure and variation of the mitochondrial genome of fishes. BMV Genomics, 17(719): 1-20. https://doi.org/10.1186/s12864-016-3054-y
Tavares, R.A.; Piedras, S.R.N.; Nunes, M.D.; Almeida, D.B.; Moreira, C.G.A.; Fernandes, J. M.; Freitas, S.F.; Moreira, H.L.M.; Pouey, J.L.O.F.; Dionello, N.J.L. 2014. Identificação de loci microssatélites com potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis). Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66(6): 1941-1945. https://doi.org/10.1590/1678-7444
Tejedor, D. 2001. El pejerrey como recurso genético. In: Grosman, Fabián. Fundamentos biológicos, económicos y sociales para una correcta gestión del recurso pejerrey. Editorial Astyanax, Buenos Aires. p. 27-31.
Wingert, J.M.; Ferrer, J.; Malabarba, L.R. 2017. Review of the Odontesthes perugiae species group from Río de La Plata drainage, with the description of a new species (Atherinomorpha: Atherinopsidae). Zootaxa, 4250(6): 501-528. https://doi.org/10.11646/zootaxa.4250.6.1
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