Production of all-female triploid rainbow trout, Oncorhynchus mykiss. I í Embryo survival

Abstract

The survival rate among all-female and mixed-sex groups of diploid and triploid rainbow trout, Oncorhynchus mykiss, during the embryonic development period was compared. AII-female groups were obtained using sperm from masculinized genotypic female with 17âˆÂ-methyltestosterone. Triploidy was induced by heat shocking the eggs at 28°C for 20 minutes, starting 10 minutes post-activation. Triploidization rate was estimated from erythrocyte major axis measurement as 94 and 100%, respectively to all-female and mixed-sex groups. Compared with the diploid control, heat-shocked eggs showed higher mortality primarily after hatching period; groups fertilized with semen from masculinized female showed higher mortality rates than groups fertilized with semen from normal males.