Motility, vigor and pathologies in fresh and cryopreserved semen of Piaractus mesopotamicus
Keywords:
cryopreservation, spermatic morphology, progressive motility, fish, spermatic vigorAbstract
Spermatozoa morphological alterations of eleven pacus (Piaractus mesopotamicus) were evaluated before and after frozen-thawed processing. The semen cryopreservation caused a decrease (P<0.05) in the percentage of normal spermatozoa compared to fresh semen from 40.21% to 31.10%. Otherwise, the percentage of spermatozoa with primary pathologies increased 14.46% (P<0.05) in the semen after thawing. However, there was no differences in seminal secondary pathologies (P>0.05) before and after cryopreservation. The progressive motility and the spermatic vigor were reduced in frozen-thawed semen in 66.45 and 34.2%, respectively (P<0.05). Moreover, bent tail abnormality and broken tail pathology were increased in 65.00 and 41.25% (P<0.05) after frozen thawed. Also, macrocephaly and distal cytoplasmatic drop were observed in the semen only after freezing, while short tail spermatozoa were observed only in fresh semen. However, a higher occurrence of loose tail was observed in fresh semen.
References
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rainbow trout sperm in large volume straws:application to large scale fertilization.
Aquaculture, Amsterdam, 201(3-4): 301-314.
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KAVAMOTO, E.T.; BARNABE, V.H.; CAMPOS,B.E.S.; TALMELLI, E.F.A. 1999
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LAHNSTEINER, F.; BERGER, B.; WEISMANN, T.;PATZNER, R. 1996 Changes in morphology, physiology, metabolism, and fertilization capacity of rainbow trout semen following cryopreservation. The Progressive FishCulturist, Bethesda, 58(3): 149-156.
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SILVEIRA, W.F.; KAVAMOTO, E.T.;CESTAROLLI, M.A.; GODINHO, H.M.;
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STREIT Jr., D.P.; MORAES, G.V.; RIBEIRO, R.P.; POVH, J.A.; SOUZA, E.D.; OLIVEIRA, C.A.L. 2004 Avaliação de diferentes técnicas para coloração de sêmen de peixes. Arquivos de Ciência Veterinárias e Zoologia da UNIPAR,Umuarama, 7(2): 157-162.
STREIT Jr. D.P.; BENITES, C.; MORAES, G.V.;RIBEIRO, R.P.; SAKAGUTI, E.S.; CALDIERI,
R.F. 2006 Sêmen de pacu (piaractus mesopotamicus) criopreservado com diluentes
utilizados para sêmen de suínos. Ciência Animal Brasileira, Goií¢nia, 7(3): 289-297.
TADDEI, A. R.; BARBATO, F.; ABELLI, L.; CANESE, S.; MORETTI, F.; RANA, K.J.;
FAUSTO, A.M.; MAZZINI, M. 2001 Is cryopreservation a homogeneous process?
Ultrastructure and motility of untreated,prefreezing, and postthawed spermatozoa of Diplodus puntazzo (Cetti). Cryobiology, San Diego, 42(4): 244-255.
TAN-FERMIN, J.D.; MIURA, T.; ADACHI, S.;YAMAUCHI, K. 1999 Seminal plasma
composition, sperm motility, milt dilution in the Asian catfish Clarias macrocephalus
(Günther). Aquaculture, Amsterdam, 171(3-4):323-338.
TIERSCH, T.R.; FIGIEL Jr., C.R.; WAYMAN, W.R.;WILLIAMSON, J.H.; CARMICHAEL, G.J.;
GORMAN, G.G. 2000 Cryopreservation of sperm of the endangered razorback sucker.
TIERSCH, T.R. e MAZIK, P.M. Cryopreservation in aquatic species. Baton Rouge: World Aquaculture Society, p.117-122.
URBÁNYI, B.; DINNYÉS, A.; MAGYARY, I. 2000 Cryopreservation methods for sperm of the sharptooth catfish. TIERSCH, T.R. e MAZIK, P.M. Cryopreservation in aquatic species. Baton Rouge: World Aquaculture Society, p.286-287.
YAO, Z.; CRIM, L.W.; RICHARDSON, G.F.;EMERSON, C.J. 2000 Motility, fertility and
ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after
cryopreservation. Aquaculture, Amsterdam,181(3-4): 361-375.
WU, A.S.H.; OLDFIELD, J.E.; SHULL, L.R.;CHEEKE, P.R. 1979 Specific effect of selenium
deficiency on rat sperm. Biology of Reproduction, Champaign, 20(4): 793-798.
BEDORE, A.G. 1999 Características e criopreservação do sêmen de pacu-caranha (Piaractus mesopotamicus) e de piracanjuba (Brycon orbignianus). Belo Horizonte. 53p. (Dissertação de Mestrado í Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais - UFMG).
BILLARD, R.; COSSON, J.; CRIM, L.W.; SUQUET, M. 1995 Broodstock management and seed quality-General considerations. In:BROMAGE, N. e ROBERTS, R.J. Broodstock
management and egg larval quality. Oxford:Blackwell Science, p.1-24.
CABRITA, E.; ROBLES, V.; ALVAREZ, R.;HERRAÉZ, M.P. 1998 Cryopreservation of
rainbow trout sperm in large volume straws:application to large scale fertilization.
Aquaculture, Amsterdam, 201(3-4): 301-314.
CAROLSFELD, J.; HARVEY, B.; SILVEIRA, W.F.;KAVAMOTO, E.T.; RAMOS, S.M.; SILVEIRA,
A.N. 1990 Criopreservação do sêmen de pacu, Piaractus mesopotamicus HOLMBERG, 1987. Boletim Técnico do CEPTA, Pirassununga,3(único): 1-4.
CAROLSFELD, J.; GODINHO, H.P.; ZANIBONI FILHO, E.; HARVEY, B. 2003 Cryopreservation of sperm in Brazilian migratory fish conservation. Journal of Fish
Biology, Londres, 63(2): 472-489.
CASTAGNOLLI, N. 1992 Espécies exóticas próprias para a piscicultura. In: CASTAGNOLLI, N. Piscicultura de água doce. Jaboticabal: FUNEP, p.71-96.
CECCARELLI, P.S.; SENHORINI, J.A.; VOLPATO, G. 2000 Dicas em piscicultura;
perguntas e respostas. Botucatu: Santina Gráfica Editora, 247p.
CHAO, N.H. e LIAO, I.C. 2001 Cryopreservation of finfish and shellfish gametes and embryos.Aquaculture, Amsterdam, 197(1): 161-189.
COSSON, J.; BILLARD, R.; CIBERT, C.;DRÉANNO, C.; SUQUET, M. 1999 Ionic factors regulating the motility of fish sperm.In: GAGNON, C. The male gamete: from basic
science to clinical applications. Viena: Cache River Press, p.162-186.
FABBROCINI, A.; LAVADERA, S.L.; RISPOLI, S.; SANSONE, G. 2000 Criopreservation of
seabream (Sparus aurata) spermatozoa.Cryobiology, San Diego, 40(10): 46-53.
FURUYA, W.M. 2001 Espécies nativas. In: MOREIRA, H.L.M.; VARGAS, L.; RIBEIRO,
R.P.; ZIMMERMANN, S. Fundamentos de Aqüicultura. Canoas: Editora da Ulbra, p.83-90.
HE, S. e WOODS III, L.C. 2003 Effects of glycine and alanine on short-term storage and cryopreservation of striped bass (Morone saxatilis) spermatozoa. Cryobiology, San Diego,46(1): 17-25.
HERMAN, H.A.; MITCHELL, J.R.; DOAK, G.A.1994 The artificial insemination and embryo transfer of diary and beef cattle. Illinois:Interstate Publishers, 382p.
KAVAMOTO, E.T.; BARNABE, V.H.; CAMPOS,B.E.S.; TALMELLI, E.F.A. 1999
Anormalidades morfológicas nos espermatozóides do curimbatá, Prochilodus
lineatus (Steindachner, 1881) (Osteichthyes, Characiformes, Prochilodontidae). Boletim do Instituto de Pesca, São Paulo, 25(único): 61-66.
LAHNSTEINER, F.; BERGER, B.; WEISMANN, T.;PATZNER, R. 1996 Changes in morphology, physiology, metabolism, and fertilization capacity of rainbow trout semen following cryopreservation. The Progressive FishCulturist, Bethesda, 58(3): 149-156.
LAHNSTEINER, F.; BERGER, B.; WEISMANN, T.; PATZNER, R. 1998 Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism.Aquaculture, Amsterdam, 163(1-2): 163-181.
RANA, K. 1995 Preservation of gametes. In:BROMAGE, N. e ROBERTS, R.J. Broodstock
management and egg larval quality. Oxford:Blackwell Science, p.53-75.
SAS INSTITUTE INC. 1992 SAS technical report.Release 6.07. Cary: NC, 229p.
SILVEIRA, W.F.; KAVAMOTO, E.T.;CESTAROLLI, M.A.; GODINHO, H.M.;
RAMOS, S.M.; SILVEIRA, A. N. 1990 Avaliação espermática, preservação criogênica
e fertilidade do sêmen do pacu, Piaractus mesopotamicus (Holmberg), proveniente de reprodução induzida. Boletim do Instituto de Pesca, São Paulo, 17(1): 1-13.
SIROL, R.N. e BRITTO, S.G. 2005 Conservação e manejo da ictiofauna: Repovoamento. In: NOGUEIRA, M.G.; HENRY, R.; JORCIN, A.Ecologia de reservatórios: Impactos potenciais, ações de manejo e sistemas em cascata. São Carlos: RIMA Editora, p.275-284.
SORENSEN Jr., A.M. 1979 A laboratory for animal reproduction. 4ºed. Massachusetts: American Press, 154p.
STREIT Jr., D.P.; MORAES, G.V.; RIBEIRO, R.P.; POVH, J.A.; SOUZA, E.D.; OLIVEIRA, C.A.L. 2004 Avaliação de diferentes técnicas para coloração de sêmen de peixes. Arquivos de Ciência Veterinárias e Zoologia da UNIPAR,Umuarama, 7(2): 157-162.
STREIT Jr. D.P.; BENITES, C.; MORAES, G.V.;RIBEIRO, R.P.; SAKAGUTI, E.S.; CALDIERI,
R.F. 2006 Sêmen de pacu (piaractus mesopotamicus) criopreservado com diluentes
utilizados para sêmen de suínos. Ciência Animal Brasileira, Goií¢nia, 7(3): 289-297.
TADDEI, A. R.; BARBATO, F.; ABELLI, L.; CANESE, S.; MORETTI, F.; RANA, K.J.;
FAUSTO, A.M.; MAZZINI, M. 2001 Is cryopreservation a homogeneous process?
Ultrastructure and motility of untreated,prefreezing, and postthawed spermatozoa of Diplodus puntazzo (Cetti). Cryobiology, San Diego, 42(4): 244-255.
TAN-FERMIN, J.D.; MIURA, T.; ADACHI, S.;YAMAUCHI, K. 1999 Seminal plasma
composition, sperm motility, milt dilution in the Asian catfish Clarias macrocephalus
(Günther). Aquaculture, Amsterdam, 171(3-4):323-338.
TIERSCH, T.R.; FIGIEL Jr., C.R.; WAYMAN, W.R.;WILLIAMSON, J.H.; CARMICHAEL, G.J.;
GORMAN, G.G. 2000 Cryopreservation of sperm of the endangered razorback sucker.
TIERSCH, T.R. e MAZIK, P.M. Cryopreservation in aquatic species. Baton Rouge: World Aquaculture Society, p.117-122.
URBÁNYI, B.; DINNYÉS, A.; MAGYARY, I. 2000 Cryopreservation methods for sperm of the sharptooth catfish. TIERSCH, T.R. e MAZIK, P.M. Cryopreservation in aquatic species. Baton Rouge: World Aquaculture Society, p.286-287.
YAO, Z.; CRIM, L.W.; RICHARDSON, G.F.;EMERSON, C.J. 2000 Motility, fertility and
ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after
cryopreservation. Aquaculture, Amsterdam,181(3-4): 361-375.
WU, A.S.H.; OLDFIELD, J.E.; SHULL, L.R.;CHEEKE, P.R. 1979 Specific effect of selenium
deficiency on rat sperm. Biology of Reproduction, Champaign, 20(4): 793-798.
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2018-11-04
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