Hematological responses in tuvira after clove oil anesthesia at different concentrations
Keywords:
alternative anesthetic, eugenol, fish hematology, live bait, Gymnotus aff. inaequilabiatusAbstract
The hematological responses in tuvira (Gymnotus aff. inaequilabiatus) after clove oil anesthesia at different concentrations were evaluated in this study. Fish (n = 25) with average length of 26.8 ± 2.4 cm and weighing 94.4 ± 23.6 g were separated in five groups composed with five animals each and submitted individually to respective concentrations of clove oil: 40, 80, 120, 160 and 200 mg L-1. After anesthetic recuperation, the blood samples collection were performed, as well as complete hemogram. The results were submitted to analysis of variance, Tukey test (P<0.05) and Sperman’s correlation. There was an increase (P<0.05) on hematocrit, red blood cells count, thrombocytes count and in the values of neutrophils with increasing of clove oil concentrations. In addition, positive correlation (P<0.05) were observed on total leukocytes, as well as negative linear correlation (P<0.05) for mean corpuscular hemoglobin concentration (MCHC). The clove oil anesthesia on tuvira causes hematological changes with an effect dose-dependent. These changes should be considered mainly in future studies designed to establish the reference values of hematologic variables for this species.
References
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VELISEK, J.; WLASOW, T.; GOMULKA, P.;SVOBODOVA, Z.; NOVOTNY, L. 2007a Efects
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VELISEK, J.; SVOBODOVA, Z.; PIACKOVA, V.2007b Effects of 2-phenoxyethanol anaesthesia on haematological profile on common carp (Cyprinus carpio) and rainbow trout(Oncorhynchus mykiss). Acta Veterinaria Brno,Brno, 76(3): 487-492.
VIDAL, L.V.O.; FURUYA, W.M.; GRACIANO, T.S.;SCHAMBER, C.R.; SANTOS, L.D.; MARTINS, C. 2007 Concentrações de eugenol para anestesia profunda e toxidade aguda em juvenis de piavuçú (Leporinus macrocephalus). Acta Scientiarum: Biological Sciences, Maringá, 29(4):357-362.
WINTROBE, M.M. 1934 Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologica,Leipzig, 51: 32-49.
WOODY, C.A.; NELSON, J.; RAMSTAD, K. 2002 Clove oil as an anesthetic for adult sockeye salmon: field trails. Journal Fish Biology, London,60(2): 340-347.
CARNEIRO, P.C.F.; URBINATH, E.C.; MARTINS,M.L. 2002 Transport with benzocaine
concentrations and its consequences on hematological parameters and gell parasite
population of matrinxã Brycon cephalus (Günher, 1869) (Oseichthyes, Characidae). Acta Scientiarum, Maringá, 24(2): 555í 560.
COLLIER, H.B. 1944 The standardizations of blood haemoglobin determinations. Canadian Medical Association Journal, Ottawa, 50(6): 550í 552.
DAVIS, A.K.; MANEY, D.L.; MAERZ, J.C. 2008 The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology, Oxford, 22(5): 760í 772.
FAí"¡ANHA, M.F. e GOMES, L.C. 2005 A eficácia do mentol como anestésico para tambaqui (Colossoma macropomum). Acta Amazonica,Manaus, 35(1): 71í 75.
FDA/CVM - Food and Drug Administration’s Center for Veterinary Medicine. 2007 Guidance for industry: concerns related to the use of clove oil as an anesthetic for fish. Rockville. 3p.Disponível em: <http://www.fda.gov/downloads/AnimalVeterinary/GuidanceComplianceEnforcement/GuidanceforIndustry/ucm052520.pdf> Acesso em: 17 jun. 2012.
GOLDENFARB, P.B.; BOWYER, F.P.; HALL, E.;BROSIOUS, E. 1971 Reproducibility in the
hematology laboratory: the microhematocrit determinations. American Journal of Clinical Pathology, Philadelphia, 56(1): 35í 39.
GONí"¡ALVES, A.F.N.; SANTOS, E.C.C.;FERNANDES, J.D.K.; TAKAHASHI, L.S. 2008
Mentol e eugenol como substitutos da benzocaína na indução anestésica de juvenis de pacu. Acta Scientiarum: Animal Sciences,Maringá, 30(3): 339í 344.
HISANO, H.; ISHIKAWA, M.M.; FERREIRA, R.A.;BULGARELLI, A.L.A.; COSTA, T.R.; PÁDUA,S.B. 2008 Tempo de indução e de recuperação de dourados Salminus brasiliensis (Cuvier, 1816),submetidos a diferentes concentrações de óleo de cravo Eugenia sp. Acta Scientiarum: Biological Sciences, Maringá, 30(3): 303-307.
INOUE, L.A.K.A.; HACKBARTH, A.; MORAES, G.2004 Avaliação dos anestésicos 2-
phenoxyethanol e benzocaína no manejo do matrinxã Brycon cephalus (Günther, 1869).Biodiversidade Pampeana, Uruguaiana, 2(1): 10-15.
INOUE, L.A.K.A.; AFONSO, L.O.B.; IWAMA, G.K.;MORAES, G. 2005. Effects of clove oil on the stress response of matrinxã (Brycon cephalus)subjected to transport. Acta Amazonica, Manaus, 35(2): 289í 295.
MARTINS, M.L.; MIYAZAKI, D.M.Y.; MORAES, F.R.; GHIRALDELLI, L.; ADAMANTE, W.B.;
MOURIí"˜O, J.L.P. 2008 Suplementação com vitamina C e E influencia a resposta inflamatória aguda em Oreochromis niloticus. Ciência Rural,Santa Maria, 38: 213-218.
MORAES, A.S.; ESPINOSA, L.W. 2001 Captura e a comercialização de iscas vivas em Corumbá-MS.Corumbá: Embrapa Pantanal, 37p. (Embrapa Pantanal. Boletim de Pesquisa, 21).
MOHAMMADIZAREJABAD, A.; BASTAMI, K.D.;SUDAGAR, M.; MOTLAGH, S.P. 2010
Haematology of great sturgeon (Huso huso Linnaeus, 1758) juvenile exposed to clove powder as an anaesthetic. Comparative Clinical Pathology, London, 19: 465í 468.
PÁDUA, S.B.; PIETRO, P.S.; IGLESSIAS-FILHO, P.S.; ISHIKAWA, M.M.; HISANO, H. 2010. Mentol como anestésico para dourado (Salminus brasiliensis). Boletim do Instituto de Pesca, São Paulo, 36(2): 143 í 148.
PEREIRA-DA-SILVA, E.M.; OLIVEIRA, R.H.F.; RIBEIRO, M.A.R.; COPOLLA, M.P. 2009 Efeito anestésico do óleo de cravo em alevinos de lambari. Ciência Rural, Santa Maria, 39(6): 1851í 1856.
ROUBACH, R.; GOMES, L.C.; FONSECA, F.A.L.;VAL, A.L. 2005 Eugenol as an efficacious
anaesthetic for tambaqui, Colossoma macropomum (Cuvier). Aquaculture Research, Oxford, 36(11):1056í 1061.
SATAKE, F.; PÁDUA, S.B.; ISHIKAWA, M.M. 2009 Distúrbios morfológicos em células sanguíneas de peixes em cultivo: uma ferramenta prognóstica. In: TAVARES-DIAS, M. Manejo e sanidade de peixes em cultivo. 1º ed. Macapá:Embrapa Amapá. p.330-345.
SNEDDON, L.U. 2004 Evolution of nociception in vertebrates: comparative analysis of lower vertebrates. Brain Research Reviews, Amsterdam,46: 123í 130.
SUDAGARA, M.; MOHAMMADIZAREJABADA, A.; MAZANDARANIA, R.; POORALIMOTLAGHA, S. 2009 The efficacy of clove powder as an anesthetic and its effects on hematological parameters on roach (Rutilus rutilus). Journal of Aquaculture Feed Science and Nutrition, Faisalabad, 1(1): 1-5.
TAVARES-DIAS, M. e MORAES, F.R. 2006 Hematological parameters for the Brycon
orbignyanus Valenciennes, 1850 (Osteichthyes,Characidae) intensively bred. Hidrobiológica,Mexico, 16: 271í 274.
VELISEK, J.; SVOBODOVA, Z.; PIACKOVA, V.;GROCH, L.; NEPEJCHALOVA, L. 2005 Effects
of clove oil on aesthesia on common carp (Cyprinus carpio L.). Veterinary Medicine, Bonner Springs, 50(6): 269í 275.
VELISEK, J.; WLASOW, T.; GOMULKA, P.;SVOBODOVA, Z.; NOVOTNY, L. 2007a Efects
of 2-phenoxyethanol anaesthesia on sheatfsh (Silurus glanis L.). Veterinary Medicine, Bonner Springs, 52(3): 103í 110.
VELISEK, J.; SVOBODOVA, Z.; PIACKOVA, V.2007b Effects of 2-phenoxyethanol anaesthesia on haematological profile on common carp (Cyprinus carpio) and rainbow trout(Oncorhynchus mykiss). Acta Veterinaria Brno,Brno, 76(3): 487-492.
VIDAL, L.V.O.; FURUYA, W.M.; GRACIANO, T.S.;SCHAMBER, C.R.; SANTOS, L.D.; MARTINS, C. 2007 Concentrações de eugenol para anestesia profunda e toxidade aguda em juvenis de piavuçú (Leporinus macrocephalus). Acta Scientiarum: Biological Sciences, Maringá, 29(4):357-362.
WINTROBE, M.M. 1934 Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologica,Leipzig, 51: 32-49.
WOODY, C.A.; NELSON, J.; RAMSTAD, K. 2002 Clove oil as an anesthetic for adult sockeye salmon: field trails. Journal Fish Biology, London,60(2): 340-347.
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2018-11-10
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