Induction of triploidy in Rhamdia quelen (Pisces, Pimelodidae)
Keywords:
triploidy, nucleolus, heat shocks, catfish, Rhamdia quelenAbstract
The triploidy induction technique in Rhamdia quelen was standardized by the application of heat shocks with different durations and in different moments post-fecundation. The survival to the heat shock was measured in the moment of larvae eclosion. In order to identify the triploid fishes, the rate of ploidy was calculated by recounting the maximum number of nucleolus in interphase nuclei. The best method to induce triploidy in Rhamdia quelen was determined based on the values of the variables analyzed in this paper. The variable that produced the greatest rate of survival to the heat shock was also indicated - temperature of the shock: 36 ± 1°C; duration of the temperature shock: 5 minutes; moment of the shock temperature: 5 minutes post-fecundation, with triploidy and survival rates of 74.8% and 33.4%, respectively.
References
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PHILLIPS, R.B.; ZAJICEK, K.D.; IHSSEN, P.E.; JOHNSON, O. 1986 Application of silver staining to the identification of triploid fish cells. Aquaculture, 54: 313-319.
PIFERRER, F.; CAL, R.; ALVAREZ-BLÁZQUEZ, B.; SÁNCHEZ, L.; MARTINEZ, P. 2000 Induction of triploidy in the turbot (Scophthalmus maximus) I. Ploidy determination and effects of cold shocks. Aquaculture, 188: 79-90.
RUDUNZ NETO, J. 1981 Desenvolvimento de técnicas de reprodução e manejo de larvas e alevinos de jundiá
(Rhamdia quelen). Santa Maria. 77p. (Dissertação de Mestrado. Curso de Pós Graduação em Zootecnia, Universidade Federal de Santa Maria- RS)
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THORGAARD, G.H 1986 Ploidy manipulation and performance. Aquaculture, 57: 57-64.
VALCARCEL, A.; GUERRERO, G.; MAGGESE, M.C. 1994 Hertwig effect caused by UVí irradiation of sperm of the catfish, Rhamdia sapo (Pisces, Pimelodidae), and its photoreactivation.Aquaculture, 128: 21-28.
Cyprinus carpio, in Karnataka, India. Aquaculture,204: 407-418.
BENFEY, T.J. y SUTTERLIN, A.M. 1984 Triploidy induced by heat shock and hydrostatic pressure in landlocked Atlantic salmon (Salmo salar L.). Aquaculture, 36(4): 359-367.
CARVALHO GOMEZ, L.; GOLOMBIESKI, J.; CHIPPARI GOMEZ, A.; BALDISSEROTO, B. 1999 Biologia do jundiá Rhamdia quelen (Telostei, Pimelodidae). Revisão bibliográfica.Ciência Rural, Santa Maria, 30:179-185.
CHERFAS, N.B.; GOMELSKY, B.I.; EMELJANOVA, O.V.; REKOUBRANISKY, A.V. 1981 Triploidy in reciprocal hybrids obtained from crucian carp and carp. Genetica, Moscow, 17(6): 1136-1139.
CHEVASSUS, B.; QUILLET, E.; CHOURROUT, D. 1983 Obtention d’animaux triploides chez la truite arc-en-ciel. Bull. Fr. Piscic., 290: 161-164.
CHEVASSUS, B.; QUILLET, E.; CHOURROUT, D. 1984 La production de truites sterilés par voie genetique. La pisciculture française, 78: 10-19.
CHOURROUT, D. 1987 Genetics manipulation in fish: Review of methods. In: WORLD SYMPOSIUM ON SELECTION, HYBRIDIZATION, AND GENETIC ENGINEERING IN AQUACULTURE, 17-30 may, 1987, Bordeaux.Anais... Berlin. v. 2
FENOCCHIO, A.S.; BERTOLLO, L.A.C.; TAKAHASHI, C.S.; CAMACHO, P.M. 2000 B chromosome in two species Genus Rhamdia (Siluriformes Pimelodidae). Folia biologica,Krakóv, 48(3-4): 105-109.
FORESTI, F.; OLIVEIRA, C.; CARVALHO, E.D. 1994 Ploidy evaluation in the pacu fish, Piaractus mesopotamicus (Pisces, Characiformes). Rev. Brasil. Biol., 54(1): 31-37.
HOWELL, W.H. y BLACK, D.A. 1980 Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a one-step method. Experientia, 36: 1014-1015.
KLIGERMAN, A.D. y BLOOM, S.E. 1977 Rapid chromosome preparations from solid tissues of fishes. J. Fish. Res. Board Can., 34: 266-269.
LINCOLN, R.F.; AULSTAD, D.; GRAMMELTVEDT,A. 1974 Attempted triploid induction in Atlantic salmon (Salmo salar) using cold shocks.Aquaculture, 4: 287-297.
LUCHINI, L. 1990 Manual para el cultivo del bagre sudamericano Rhamdia sapo. Santiago de Chile:RLAC/90/16- PES-20, FAO. 60p.
NARAHARA, M.Y.; GODINHO, H.M.; FENERICHVERANI, N.; ROMAGOSA, E. 1985a Relação peso-comprimento e fator de condição de Rhamdia hilarii (Valenciennes, 1840) (Osteichthyes, Siluriformes, Pimelodidae).B. Inst. Pesca, 12(4): 13-22.
NARAHARA, M.Y.; GODINHO, H.M.; ROMAGOSA, E. 1985b Estrutura da população de Rhamdia hilarii Valenciennes, 1840) (Osteichthyes, Siluriformes, Pimelodidae). B. Inst. Pesca,12(3): 123-137.
PÉREZ CARRASCO, L.A.; PENMAN, D.J.;BROMAGE, N. 1999 Parámetros morfométricos de interés comercial en trucha arcoiris triploide, Oncorhynchus mykiss. Revista Aquatic, 6: febrero. Disponible el 10/04/2003 en URL:www.revistaaquatic.com
PHILLIPS, R.B.; ZAJICEK, K.D.; IHSSEN, P.E.; JOHNSON, O. 1986 Application of silver staining to the identification of triploid fish cells. Aquaculture, 54: 313-319.
PIFERRER, F.; CAL, R.; ALVAREZ-BLÁZQUEZ, B.; SÁNCHEZ, L.; MARTINEZ, P. 2000 Induction of triploidy in the turbot (Scophthalmus maximus) I. Ploidy determination and effects of cold shocks. Aquaculture, 188: 79-90.
RUDUNZ NETO, J. 1981 Desenvolvimento de técnicas de reprodução e manejo de larvas e alevinos de jundiá
(Rhamdia quelen). Santa Maria. 77p. (Dissertação de Mestrado. Curso de Pós Graduação em Zootecnia, Universidade Federal de Santa Maria- RS)
SILFVERGRIP, A.M.C. 1996 A systematic revision of the neotropical catfish genus Rhamdia (Telostei, Pimelodidae). Stockholm, Sweden. 157p. [PhD.Thesis. (Tese de Doutoramento.)].
THORGAARD, G.H 1986 Ploidy manipulation and performance. Aquaculture, 57: 57-64.
VALCARCEL, A.; GUERRERO, G.; MAGGESE, M.C. 1994 Hertwig effect caused by UVí irradiation of sperm of the catfish, Rhamdia sapo (Pisces, Pimelodidae), and its photoreactivation.Aquaculture, 128: 21-28.
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2018-07-02
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