Effect of nitrate depletion on lipid accumulation by the marine microalga Nannochloropsis oculata
Keywords:
biomass, lipid content, cells yieldAbstract
Nannochloropsis oculata is a marine algae with high lipid content, being a promising species for biofuel production. It is used also as food in aquaculture for larval fish and crustaceans due to its high nutritional value and compatible size with the larvae feeding. This study evaluated the lipid content in the biomass of N. oculata under nitrate depletion. The cultivation was carried out in 8 L bottles with three replications, at salinity 27, illuminance of 60 μE cm-2 s-1 and temperature 28°C. The levels of sodium nitrate used were 15; 30; 45; 60 and 75 mg L-1, while maintaining constant the levels of all other nutrients of the Guillard f/2 medium. The growth curve was monitored every two days by spectrophotometry at 680 nm (DO680nm) and cell counting in a Neubauer chamber. For separation of the culture medium from the cells, we used chemical flocculation method adding 2N NaOH. The higher lipid content in the biomass was obtained at the lowest level of nitrate in the culture medium.
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using supercritical carbon dioxide extraction.Process Biochemistry, 45(12): 1865-1872.
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accumulation and lipid property in Nannochloropsis oculata for biodiesel production. Bioresource Technology, 147: 534-538
for oil producing microalgal strains: Evaluation of oil and biomass production for ten microalgal
strains. Bioresource Technology, 102(8): 5248-5250.
BLIGH, E.G. and DYER, W.J. 1959 A rapid method of total lipid extraction and purification. Canadian
Journal of Biochemistry and Physiology, 37(8): 911-917.
CHARITY, E.A.R.; ALEXANDRA, L.V.B.; CARMEN, H.C.L.; EVER, D.M.A. 2009 Biomass production
of microalga Scenedesmus sp. with wastewater from fishery. Revista Tecnica de la Facultad de
Ingenieria Universidad del Zulia, 32(2): 126-134.
CHEN, M.; TANG, H.; MA, H.; HOLLAND, T.C.; SIMON, K.Y.; SALLEY, S.O. 2011 Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresource Technology, 102(2): 1649-1655.
CHIU, S.; KAO, C.; TSAI, M.; ONG, S.; CHEN, C.; LIN, C. 2009 Lipid accumulation and CO2
utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresource Technology, 100(2):833-838.
CONVERTI, A.; CASAZZA, A.A.; ORTIZ, E.Y.;PEREGO, P.; DEL BORGHI, M. 2009 Effect of
temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis
oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing:
Process Intensification, 48(6): 1146-1151.
DE LA HOZ, S.; BEN-ZVI, A.; BURRELL, R.E.; McCAFFREY, W.C. 2011 The dynamics of heterotrophic algal cultures. Bioresource Technology, 102(10): 5764-5774.
DRAGONE, G.; FERNANDES, B.D.; ABREU, A.P.; VICENTE, A.A.; TEIXEIRA, J.A. 2011 Nutrient
limitation as a strategy for increasing starch accumulation in microalgae. Applied Energy,
88(10): 3331-3335.
ELLIS, J.T.; HENGGE, N.N.; SIMS, R.C.; MILLER, C.D. 2012 Acetone, butanol, and ethanol
production from wastewater algae. Bioresource Technology, 111: 491-495.
GRIS, L.R.S.; PAIM, A.C.; FARENZENA, M; TRIERWEILER, J.O. 2013 Influence of NaNO3 concentration and incident light intensity on Nannochloropsis oculata lipid accumulation. Brazilian Archives of Biology and Technology, 56(4):673-678.
GUEDES, A.C.; AMARO, H.M.; MALCATA, F.X. 2011 Microalgae as sources of high added-value
compounds - a brief review of recent work. Biotechnology Progress, 27(3): 597-613.
GUILLARD, R.R.L. 1975 Culture of phytoplankton for feeding marine invertebrates. In: SMITH,
W.L. and CHANLEY, M.H. Culture of marine invertebrate animal. New York: Plenum Publishing.
p.29-60.
JIANG-MING, L.; LI-HUA, C.; XIN-HUA, X.; LIN, Z.; HUAN-LIN, Z. 2010 Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresource Technology, 101(17): 6797-6804.
KONG, Q.; ZHU, L.; SHEN, X. 2010 The toxicity of naphthalene to marine Chlorella vulgaris under
different nutrient conditions. Journal of Hazardous Materials, 178(1-3): 282-286.
LEVINE, R.B.; COSTANZA-ROBINSON, M.S.; SPATAFORA, G.A. 2011 Neochloris oleoabundans
grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel
feedstock production. Biomass and Bioenergy,35(1): 40-49.
LI, Y.; CHEN, Y-F.; CHEN, P.; MIN, M.; ZHOU, W.;MARTINEZ, B.; ZHU, J.; RUAN, R. 2011 Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal
wastewater for nutrient removal and biodiesel production. Bioresource Technology, 102(8):
5138-5144.
LIN, Q.; GU, N.; LI, G.; LIN, J.; HUANG, L.; TAN, L. 2012 Effects of inorganic carbon concentration
on carbon formation, nitrate utilization, biomass and oil accumulation of Nannochloropsis oculata
CS 179. Bioresource Technology, 111: 353-359.
MASSART, A.; AUBRY, E.; HAMTSON, A.L. 2010 Study of culture strategies of Dunaliella tertiolecta
combining high cell density and accumulation of lipids to produce biodiesel. Biotechnology,
Agronomy and Society and Environment, 14(2): 567-572.
RODOLFI, L.; ZITTELLI, G.C.; BASSI, N.; PADOVANI, G.; BIONDI, N.; BONINI, G.; TREDICI, M.R. 2009 Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnology and Bioengineering,102(1): 100-112.
RODRIGUES, J.A.G.; SOUSA JR., J.; LOURENí"¡O J.A.; LIMA P.C.W.C.; FARIAS, W.R.L. 2009 Cultivo de camarões tratados com polissacarídeos sulfatados da rodofícea Halymenia pseudofloresia mediante uma estratégia profilática. Ciência Agronômica, 40(1): 71-78.
TAMBURIC, B.; SZABÓ, M.; TRAN, N.T.; LARKUM, A.W.D.; SUGGETT, D.J.; RALPH, P.J. 2014 Action spectra of oxygen production and chlorophylla fluorescence in the green microalga Nannochloropsis oculata. Bioresource Technology, 169: 320-327.
WANG, H.M.; PAN, J.L.; CHEN, C.Y.; CHIU, C.C.;YANG, M.H.; CHANG, H.W.; CHANG, J.S. 2010
Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property
using supercritical carbon dioxide extraction.Process Biochemistry, 45(12): 1865-1872.
WEI, L.; HUANG, X.; HUANG, Z.; ZHOU, Z. 2013 Orthogonal test design for optimization of lipid
accumulation and lipid property in Nannochloropsis oculata for biodiesel production. Bioresource Technology, 147: 534-538
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2018-11-22
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