Efeito da depleção de nitrato no acúmulo lipídico da microalga marinha Nannochloropsis oculata
Palavras-chave:
biomassa, conteúdo de lipídios, rendimento celularResumo
Nannochloropsis oculata é uma microalga marinha que possui alto teor lipídico, sendo uma promissora espécie para a produção de biocombustível. É utilizada também como alimento na aquicultura na fase larval de peixes e crustáceos devido ao seu elevado valor nutricional e por possuir tamanho compatível com o mecanismo alimentar das larvas. O objetivo do trabalho foi avaliar o rendimento lipídico da N. oculata sob depleção de nitrato. Os cultivos foram realizados em frascos de 8 L, com três repetições, na salinidade de 27, iluminí¢ncia de 60 μE cm-2 s-1 e temperatura de 28°C. As quantidades de nitrato de sódio utilizadas foram 15; 30; 45; 60 e 75 mg L-1, mantendo constantes as quantidades dos outros nutrientes do meio Guillard f/2. A curva de crescimento foi acompanhada a cada dois dias por espectrofotometria a 680 nm (DO680nm) e contagem celular em cí¢mara de Neubauer. Para separação do meio de cultivo das células foi utilizado o método de floculação química, adicionando NaOH 2N. O melhor rendimento lipídico foi obtido na menor quantidade de nitrato no meio de cultura.
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CHARITY, E.A.R.; ALEXANDRA, L.V.B.; CARMEN, H.C.L.; EVER, D.M.A. 2009 Biomass production
of microalga Scenedesmus sp. with wastewater from fishery. Revista Tecnica de la Facultad de
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CHEN, M.; TANG, H.; MA, H.; HOLLAND, T.C.; SIMON, K.Y.; SALLEY, S.O. 2011 Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresource Technology, 102(2): 1649-1655.
CHIU, S.; KAO, C.; TSAI, M.; ONG, S.; CHEN, C.; LIN, C. 2009 Lipid accumulation and CO2
utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresource Technology, 100(2):833-838.
CONVERTI, A.; CASAZZA, A.A.; ORTIZ, E.Y.;PEREGO, P.; DEL BORGHI, M. 2009 Effect of
temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis
oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing:
Process Intensification, 48(6): 1146-1151.
DE LA HOZ, S.; BEN-ZVI, A.; BURRELL, R.E.; McCAFFREY, W.C. 2011 The dynamics of heterotrophic algal cultures. Bioresource Technology, 102(10): 5764-5774.
DRAGONE, G.; FERNANDES, B.D.; ABREU, A.P.; VICENTE, A.A.; TEIXEIRA, J.A. 2011 Nutrient
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88(10): 3331-3335.
ELLIS, J.T.; HENGGE, N.N.; SIMS, R.C.; MILLER, C.D. 2012 Acetone, butanol, and ethanol
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GUEDES, A.C.; AMARO, H.M.; MALCATA, F.X. 2011 Microalgae as sources of high added-value
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KONG, Q.; ZHU, L.; SHEN, X. 2010 The toxicity of naphthalene to marine Chlorella vulgaris under
different nutrient conditions. Journal of Hazardous Materials, 178(1-3): 282-286.
LEVINE, R.B.; COSTANZA-ROBINSON, M.S.; SPATAFORA, G.A. 2011 Neochloris oleoabundans
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wastewater for nutrient removal and biodiesel production. Bioresource Technology, 102(8):
5138-5144.
LIN, Q.; GU, N.; LI, G.; LIN, J.; HUANG, L.; TAN, L. 2012 Effects of inorganic carbon concentration
on carbon formation, nitrate utilization, biomass and oil accumulation of Nannochloropsis oculata
CS 179. Bioresource Technology, 111: 353-359.
MASSART, A.; AUBRY, E.; HAMTSON, A.L. 2010 Study of culture strategies of Dunaliella tertiolecta
combining high cell density and accumulation of lipids to produce biodiesel. Biotechnology,
Agronomy and Society and Environment, 14(2): 567-572.
RODOLFI, L.; ZITTELLI, G.C.; BASSI, N.; PADOVANI, G.; BIONDI, N.; BONINI, G.; TREDICI, M.R. 2009 Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnology and Bioengineering,102(1): 100-112.
RODRIGUES, J.A.G.; SOUSA JR., J.; LOURENí"¡O J.A.; LIMA P.C.W.C.; FARIAS, W.R.L. 2009 Cultivo de camarões tratados com polissacarídeos sulfatados da rodofícea Halymenia pseudofloresia mediante uma estratégia profilática. Ciência Agronômica, 40(1): 71-78.
TAMBURIC, B.; SZABÓ, M.; TRAN, N.T.; LARKUM, A.W.D.; SUGGETT, D.J.; RALPH, P.J. 2014 Action spectra of oxygen production and chlorophylla fluorescence in the green microalga Nannochloropsis oculata. Bioresource Technology, 169: 320-327.
WANG, H.M.; PAN, J.L.; CHEN, C.Y.; CHIU, C.C.;YANG, M.H.; CHANG, H.W.; CHANG, J.S. 2010
Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property
using supercritical carbon dioxide extraction.Process Biochemistry, 45(12): 1865-1872.
WEI, L.; HUANG, X.; HUANG, Z.; ZHOU, Z. 2013 Orthogonal test design for optimization of lipid
accumulation and lipid property in Nannochloropsis oculata for biodiesel production. Bioresource Technology, 147: 534-538
for oil producing microalgal strains: Evaluation of oil and biomass production for ten microalgal
strains. Bioresource Technology, 102(8): 5248-5250.
BLIGH, E.G. and DYER, W.J. 1959 A rapid method of total lipid extraction and purification. Canadian
Journal of Biochemistry and Physiology, 37(8): 911-917.
CHARITY, E.A.R.; ALEXANDRA, L.V.B.; CARMEN, H.C.L.; EVER, D.M.A. 2009 Biomass production
of microalga Scenedesmus sp. with wastewater from fishery. Revista Tecnica de la Facultad de
Ingenieria Universidad del Zulia, 32(2): 126-134.
CHEN, M.; TANG, H.; MA, H.; HOLLAND, T.C.; SIMON, K.Y.; SALLEY, S.O. 2011 Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresource Technology, 102(2): 1649-1655.
CHIU, S.; KAO, C.; TSAI, M.; ONG, S.; CHEN, C.; LIN, C. 2009 Lipid accumulation and CO2
utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresource Technology, 100(2):833-838.
CONVERTI, A.; CASAZZA, A.A.; ORTIZ, E.Y.;PEREGO, P.; DEL BORGHI, M. 2009 Effect of
temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis
oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing:
Process Intensification, 48(6): 1146-1151.
DE LA HOZ, S.; BEN-ZVI, A.; BURRELL, R.E.; McCAFFREY, W.C. 2011 The dynamics of heterotrophic algal cultures. Bioresource Technology, 102(10): 5764-5774.
DRAGONE, G.; FERNANDES, B.D.; ABREU, A.P.; VICENTE, A.A.; TEIXEIRA, J.A. 2011 Nutrient
limitation as a strategy for increasing starch accumulation in microalgae. Applied Energy,
88(10): 3331-3335.
ELLIS, J.T.; HENGGE, N.N.; SIMS, R.C.; MILLER, C.D. 2012 Acetone, butanol, and ethanol
production from wastewater algae. Bioresource Technology, 111: 491-495.
GRIS, L.R.S.; PAIM, A.C.; FARENZENA, M; TRIERWEILER, J.O. 2013 Influence of NaNO3 concentration and incident light intensity on Nannochloropsis oculata lipid accumulation. Brazilian Archives of Biology and Technology, 56(4):673-678.
GUEDES, A.C.; AMARO, H.M.; MALCATA, F.X. 2011 Microalgae as sources of high added-value
compounds - a brief review of recent work. Biotechnology Progress, 27(3): 597-613.
GUILLARD, R.R.L. 1975 Culture of phytoplankton for feeding marine invertebrates. In: SMITH,
W.L. and CHANLEY, M.H. Culture of marine invertebrate animal. New York: Plenum Publishing.
p.29-60.
JIANG-MING, L.; LI-HUA, C.; XIN-HUA, X.; LIN, Z.; HUAN-LIN, Z. 2010 Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresource Technology, 101(17): 6797-6804.
KONG, Q.; ZHU, L.; SHEN, X. 2010 The toxicity of naphthalene to marine Chlorella vulgaris under
different nutrient conditions. Journal of Hazardous Materials, 178(1-3): 282-286.
LEVINE, R.B.; COSTANZA-ROBINSON, M.S.; SPATAFORA, G.A. 2011 Neochloris oleoabundans
grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel
feedstock production. Biomass and Bioenergy,35(1): 40-49.
LI, Y.; CHEN, Y-F.; CHEN, P.; MIN, M.; ZHOU, W.;MARTINEZ, B.; ZHU, J.; RUAN, R. 2011 Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal
wastewater for nutrient removal and biodiesel production. Bioresource Technology, 102(8):
5138-5144.
LIN, Q.; GU, N.; LI, G.; LIN, J.; HUANG, L.; TAN, L. 2012 Effects of inorganic carbon concentration
on carbon formation, nitrate utilization, biomass and oil accumulation of Nannochloropsis oculata
CS 179. Bioresource Technology, 111: 353-359.
MASSART, A.; AUBRY, E.; HAMTSON, A.L. 2010 Study of culture strategies of Dunaliella tertiolecta
combining high cell density and accumulation of lipids to produce biodiesel. Biotechnology,
Agronomy and Society and Environment, 14(2): 567-572.
RODOLFI, L.; ZITTELLI, G.C.; BASSI, N.; PADOVANI, G.; BIONDI, N.; BONINI, G.; TREDICI, M.R. 2009 Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnology and Bioengineering,102(1): 100-112.
RODRIGUES, J.A.G.; SOUSA JR., J.; LOURENí"¡O J.A.; LIMA P.C.W.C.; FARIAS, W.R.L. 2009 Cultivo de camarões tratados com polissacarídeos sulfatados da rodofícea Halymenia pseudofloresia mediante uma estratégia profilática. Ciência Agronômica, 40(1): 71-78.
TAMBURIC, B.; SZABÓ, M.; TRAN, N.T.; LARKUM, A.W.D.; SUGGETT, D.J.; RALPH, P.J. 2014 Action spectra of oxygen production and chlorophylla fluorescence in the green microalga Nannochloropsis oculata. Bioresource Technology, 169: 320-327.
WANG, H.M.; PAN, J.L.; CHEN, C.Y.; CHIU, C.C.;YANG, M.H.; CHANG, H.W.; CHANG, J.S. 2010
Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property
using supercritical carbon dioxide extraction.Process Biochemistry, 45(12): 1865-1872.
WEI, L.; HUANG, X.; HUANG, Z.; ZHOU, Z. 2013 Orthogonal test design for optimization of lipid
accumulation and lipid property in Nannochloropsis oculata for biodiesel production. Bioresource Technology, 147: 534-538
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2018-11-22
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