Pcr-rflp for identification of the pearl oyster Pinctada imbricate from Brazil and Venezuela
DOI:
https://doi.org/10.20950/1678-2305.2017v43n3p459Keywords:
oyster, Pinctada imbricata, South America, Pearl oysterAbstract
Oysters of the genus Pinctada are of great economic importance due to their extensive use in human feeding and pearl cultivation. It includes four species: Pinctada radiata (Europe), P. imbricata (Western Atlantic) and the P. fucata-martensi complex (Pacific), the latter being a species complex of difficult morphological differentiation. Although this species complex has several molecular studies corroborating each species as valid, there are still doubts about the validity of P. imbricata in the South of Western Atlantic (i.e., Brazilian Coast). Here we carried out a RFLP study with populations from Ceará, Rio de Janeiro, São Paulo and Venezuelan coast. We analyzed mitochondrial (16S) and nuclear genes (partial IGS). This study confirms the Brazilian and Venezuelan stocks as genetically close to the P. imbricata stocks from Caribbean than the P. martensi-fucata complex. This result is important for pearl-oyster farmers, demonstrating that the Brazilian and Venezuelan stocks are not alien species or hybrids of Indo-Pacific species.
References
ALFAYA, J.; BIGATTI, G.; MACHORDOM. A. 2013 Mitochondrial and nuclear markers reveal a
lack of genetic structure in the entocommensal nemertean Malacobdella arrokeana in the
Patagonian gulfs. Helgoland Marine Research,67(2): 407-412.
ALJANABI, S.M.; MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic
DNA for PCR-based techniques. Nucleic Acids Research, 25(22): 4692-4693.
ARNAUD, S.; MONTEFORTE, M.; GALTIER, N.;BONHOMME, F.; BLANC, F. 2000 Population
structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from
Mexico to Panama. Conservation Genetics, 1(1):299-308.
ARNAUD-HAOND, S.; BLANC, F.; BONHOMME,F.; MONTEFORTE, M. 2005 Recent foundation
of Mexican populations of pearl oysters (Pteria sterna) revealed by lack of genetic variation on
two mitochondrial genes. Journal of the Marine Biological Association of the United Kingdom UK,
85(3): 363-366.
BOUCHET, P.; GOFAS, S. 2013 Pinctada radiata (Leach, 1814). World Register of Marine Species
[online] URL: http://www.marinespecies.org/aphia.php?p=taxdetails&id=140890 on 2016-06-16.
CUNHA, R.L.; BLANC, F.; BONHOMME, F.;ARNAUD-HAOND, S. 2011 Evolutionary patterns in pearl oysters of the genus Pinctada (Bivalvia: Pteriidae). Marine Biotechnolgy, 13(1):181-192.
DE BIASI, J.B.; TOMÁS, A.R.G.; HILSDORF, A.W.S.2016 Molecular evidence of two cryptic species
of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil. Genetics and Molecular Biology, 39(3): 392-397.
GUZMÁN, B.E.; NUí"˜EZ, J.J.; VEJAR, A.; BARRIGA, E.H.; GALLARDO, C.S. 2011 Genetic diversity
and population structure of two South American marine gastropods; Crepipatella dilatata and C.
fecunda (Gastropoda: Calyptraeidae): distinct patterns based on developmental mode. Italian
Journal of Zoology, 78(4): 444-454.
GWAK, W.S.; NAKAYAMA, K. 2011 Genetic variation of hatchery and wild stocks of the pearl oyster Pinctada fucata martensii (Dunker, 1872), assessed by mitochondrial DNA analysis.Aquaculture International, 19(5): 585-591.
HUVET, A., LAPEGUE, S., MAGOULAS, A., BOUDRY, P. 2000. Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe. Conservation Genetic, 1(1): 251-262.
LIU, Z. J.; CORDES, J. F. 2004 DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238(1-4): 1-37.
MARCANO, J. S.; PRIETO, A.; LÁREZ, A.; ALIÓ, J.; SANABRIA, H. 2005. Crecimiento y mortalidad
de Pinctada imbricata (Mollusca: Pteriidae) en Guamachito, Península de Araya, Estado Sucre,
Venezuela. Ciencias Marinas, 31(3): 387-397.
MARQUES, R.C.; BREVES, A. 2015 First record of Pinctada imbricata Rí¶ding, 1798 (Bivalvia:
Pteroidea) attached to a rafting item: a potentially invasive species on the Uruguayan coast. Marine Biodiversity, 45(3): 333-337.
MARQUES, R.C.; BARBIERI, E. 2015 Anatomical differences among specimens of Pinctada
imbricata Rí¶ding, 1798 from different south American localities. Boletim do Instituto de Pesca,
42(esp): 53-63.
MARTINEZ-FERNANDEZ, E.; SOUTHGATE, P. C. 2007 Use tropical microalge as foot for larvae
of the Black-lip perl oyster Pinctada margarifera. Aquaculture, 263(1-4): 220-226.
MASAOKA, T.; KOBAYASHI, T. 2005 Species identification of Pinctada imbricata using intergenic spacer of nuclear ribosomal RNA genes and mitochondrial 16S ribosomal RNA gene regions. Fisheries Science, 71(4): 837-846.
O’CONNOR, W.A.; LAWLER, N.F. (2004) Salinity and temperature tolerance of embryos and juveniles of the pearl oyster Pinctada imbricata Roding. Aquaculture, 229(1-4): 493- 506.
RIOS, E.C. 2009 Compendium of Brazilian Sea shells. Rio Grande, Evangraf. 676 p.
SHIRAI S., 1994 Pearls and pearl oysters of the world. Marine Planning Co, Okinawa.108 p.
SOUTHGATE, P.C. 2007 Overview of the cultured marine pearl industry. In: M.G. BONDAD-REANTASO; S.E. MCGLADDERY; AND F.C.J. BERTHE. Pearl oyster health management: a manual. FAO Fisheries Technical Paper. Nº 503. FAO, Rome. p. 7-17.
Tí"¹MKIN, I. 2010 Molecular phylogeny of pearl oysters and their relatives (Mollusca, Bivalvia,
Pterioidea). BMC Evolutionary Biology, 10(1): 1-28.
URBAN, H.J. 2000 Culture potential of the pearl oyster (Pinctada imbricata) from the Caribbean.:
I. Gametogenic activity, growth, mortality and production of a natural population. Aquaculture,
189(1-4): 361-373.
VILLALOBOS, L.B.; ELGUEZABAL, L.A. 2001 Microbiological quality of the bivalve Pinctada
imbricata commercialised in Cumana, Venezuela. Acta Cientifica Venezolana 52(1): 55-61.
lack of genetic structure in the entocommensal nemertean Malacobdella arrokeana in the
Patagonian gulfs. Helgoland Marine Research,67(2): 407-412.
ALJANABI, S.M.; MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic
DNA for PCR-based techniques. Nucleic Acids Research, 25(22): 4692-4693.
ARNAUD, S.; MONTEFORTE, M.; GALTIER, N.;BONHOMME, F.; BLANC, F. 2000 Population
structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from
Mexico to Panama. Conservation Genetics, 1(1):299-308.
ARNAUD-HAOND, S.; BLANC, F.; BONHOMME,F.; MONTEFORTE, M. 2005 Recent foundation
of Mexican populations of pearl oysters (Pteria sterna) revealed by lack of genetic variation on
two mitochondrial genes. Journal of the Marine Biological Association of the United Kingdom UK,
85(3): 363-366.
BOUCHET, P.; GOFAS, S. 2013 Pinctada radiata (Leach, 1814). World Register of Marine Species
[online] URL: http://www.marinespecies.org/aphia.php?p=taxdetails&id=140890 on 2016-06-16.
CUNHA, R.L.; BLANC, F.; BONHOMME, F.;ARNAUD-HAOND, S. 2011 Evolutionary patterns in pearl oysters of the genus Pinctada (Bivalvia: Pteriidae). Marine Biotechnolgy, 13(1):181-192.
DE BIASI, J.B.; TOMÁS, A.R.G.; HILSDORF, A.W.S.2016 Molecular evidence of two cryptic species
of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil. Genetics and Molecular Biology, 39(3): 392-397.
GUZMÁN, B.E.; NUí"˜EZ, J.J.; VEJAR, A.; BARRIGA, E.H.; GALLARDO, C.S. 2011 Genetic diversity
and population structure of two South American marine gastropods; Crepipatella dilatata and C.
fecunda (Gastropoda: Calyptraeidae): distinct patterns based on developmental mode. Italian
Journal of Zoology, 78(4): 444-454.
GWAK, W.S.; NAKAYAMA, K. 2011 Genetic variation of hatchery and wild stocks of the pearl oyster Pinctada fucata martensii (Dunker, 1872), assessed by mitochondrial DNA analysis.Aquaculture International, 19(5): 585-591.
HUVET, A., LAPEGUE, S., MAGOULAS, A., BOUDRY, P. 2000. Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe. Conservation Genetic, 1(1): 251-262.
LIU, Z. J.; CORDES, J. F. 2004 DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238(1-4): 1-37.
MARCANO, J. S.; PRIETO, A.; LÁREZ, A.; ALIÓ, J.; SANABRIA, H. 2005. Crecimiento y mortalidad
de Pinctada imbricata (Mollusca: Pteriidae) en Guamachito, Península de Araya, Estado Sucre,
Venezuela. Ciencias Marinas, 31(3): 387-397.
MARQUES, R.C.; BREVES, A. 2015 First record of Pinctada imbricata Rí¶ding, 1798 (Bivalvia:
Pteroidea) attached to a rafting item: a potentially invasive species on the Uruguayan coast. Marine Biodiversity, 45(3): 333-337.
MARQUES, R.C.; BARBIERI, E. 2015 Anatomical differences among specimens of Pinctada
imbricata Rí¶ding, 1798 from different south American localities. Boletim do Instituto de Pesca,
42(esp): 53-63.
MARTINEZ-FERNANDEZ, E.; SOUTHGATE, P. C. 2007 Use tropical microalge as foot for larvae
of the Black-lip perl oyster Pinctada margarifera. Aquaculture, 263(1-4): 220-226.
MASAOKA, T.; KOBAYASHI, T. 2005 Species identification of Pinctada imbricata using intergenic spacer of nuclear ribosomal RNA genes and mitochondrial 16S ribosomal RNA gene regions. Fisheries Science, 71(4): 837-846.
O’CONNOR, W.A.; LAWLER, N.F. (2004) Salinity and temperature tolerance of embryos and juveniles of the pearl oyster Pinctada imbricata Roding. Aquaculture, 229(1-4): 493- 506.
RIOS, E.C. 2009 Compendium of Brazilian Sea shells. Rio Grande, Evangraf. 676 p.
SHIRAI S., 1994 Pearls and pearl oysters of the world. Marine Planning Co, Okinawa.108 p.
SOUTHGATE, P.C. 2007 Overview of the cultured marine pearl industry. In: M.G. BONDAD-REANTASO; S.E. MCGLADDERY; AND F.C.J. BERTHE. Pearl oyster health management: a manual. FAO Fisheries Technical Paper. Nº 503. FAO, Rome. p. 7-17.
Tí"¹MKIN, I. 2010 Molecular phylogeny of pearl oysters and their relatives (Mollusca, Bivalvia,
Pterioidea). BMC Evolutionary Biology, 10(1): 1-28.
URBAN, H.J. 2000 Culture potential of the pearl oyster (Pinctada imbricata) from the Caribbean.:
I. Gametogenic activity, growth, mortality and production of a natural population. Aquaculture,
189(1-4): 361-373.
VILLALOBOS, L.B.; ELGUEZABAL, L.A. 2001 Microbiological quality of the bivalve Pinctada
imbricata commercialised in Cumana, Venezuela. Acta Cientifica Venezolana 52(1): 55-61.
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Published
2017-09-25
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