Routine exposure to biometric procedures in fish farming reveals differences in stress response in tambaqui and hybrid tambatinga
DOI:
https://doi.org/10.20950/1678-2305.2017.1.10Keywords:
Colossoma sp, cortisol, handling, hematology, fish, Piaractus spAbstract
The physiological stress responses of tambaqui and hybrid tambatinga were evaluated after subjecting the fish to routine practices in a breeding system such as periodic biometric procedures. For 270 days of culture, the fish underwent monthly biometric measurements, and, at the end of the period, blood was collected at six sampling times (before, immediately after, and 2, 24, 48 and 72 h after biometric measurements) for the evaluation of physiological indicators of stress. Tambatinga is more susceptible to stress because it presented higher levels of cortisol and glucose in the bloodstream after handling and took longer to recover its basal physiological state for these parameters. However, the low cortisol levels observed in both species suggest that the fish were familiar with biometric procedures, resulting in a less intense response. Handling led to an increase in the cellular volume of erythrocytes in tambaqui, resulting in a change in hematocrit and a decrease in hemoglobin concentration. Hypochloremia was found in both species only 72 h after handling. Biometric procedures promote hormonal, hematological and hydroelectrolytic changes in the tambaqui and hybrid tambatinga, but when routinely adopted, at regular intervals, they elicit stress responses of lower magnitude.
References
ABREU, J.S.; URBINATI, E.C. 2006 Physiological responses of matrinxã (Brycon amazonicus) fed
different levels of vitamin C and submitted to air exposure. Acta Amazonica, 36(4): 519-524.
ABREU, J.S.; TAKAHASHI, L.S.; HOSHIBA, M.A.; URBINATI, E.C. 2009 Biological indicators of
stress in pacu (Piaractus mesopotamicus) after capture. Brazilian Journal of Biology, 69(2): 415-421.
ARAíÅ¡JO-LIMA, C.A.R.M.; GOMES, L.C. 2010 Tambaqui (Colossoma macropomum). In: BALDISSEROTTO, B.; GOMES, L.C. Espécies nativas para piscicultura no Brasil. Ed. Santa
Maria: UFSM, p.175-204.
BARTON, B.A. 2002 Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42(3): 517-525.
BARTON, B.A.; SCHRECK, C.B.; BARTON, L.D. 1987 Effects of chronic cortisol administration
and daily acute stress on growth, physiological conditions, and stress responses in juvenile
rainbow trout. Diseases of Aquatic Organisms, 2: 173-185.
BOSISIO, F.; REZENDE, K.F.O.; BARBIERI, E. 2017 Alterations in the hematological parameters of
Juvenile Nile Tilapia (Oreochromis niloticus) submitted to different salinities. Pan-American
Journal of Aquatic Sciences, 12(2): 146-154.
CHAGAS, E.C.; BOIJINK, C.L.; INOUE, L.A.K.A.; ARAíÅ¡JO, L.D.; GOMES, L.C.; MORAES, F.R.
2012 Tambaqui responses to stress due to transport after feeding with β-glucan supplemented diets. Biotemas, 25(4): 221-227.
EMERSON, K.; RUSSO, R.C.; LUND, R.E.; THURSTON, R.V. 1975 Aqueous ammonia equilibrium calculations: Effect of pH and temperature. Journal of the Fisheries Research
Board of Canada, 32(12): 2379-2383.
FAGUNDES, M.; URBINATI, E.C. 2008 Stress in pintado (Pseudoplatystoma corruscans) during
farming procedures. Aquaculture, 276(1-4): 112-119.
FEITOSA, K.C.O.; POVH, J.A.; ABREU, J.S. 2013 Physiological responses of pacu (Piaractus
mesopotamicus) treated with homeopathic product and submitted to transport stress.
Homeopathy, 102(4): 268-273.
GAMPERL, A.K.; VIJAYAN, M.M.; BOUTILIER, R.G. 1994 Experimental control of stress hormone
levels in fishes: techniques and applications. Reviews in Fish Biology and Fisheries, 4(2): 215-255.
GOMES, L.C.; ARAUJO-LIMA, C.A.R.M.; ROUBACH,R.; URBINATI, E.C. 2003a Avaliação dos efeitos
da adição de sal e da densidade no transporte de tambaqui. Pesquisa Agropecuária Brasileira, 38(2): 283-290.
GOMES, L.C.; ROUBACH, R.; CAVERO, B.A.S.; PEREIRA-FILHO, M.; URBINATI, E.C. 2003b
Transport of pirarucu Arapaima gigas juveniles in plastic bag. Acta Amazonica, 33(4): 637-642.
GOMES, L.C.; ROUBACH, R.; ARAUJO -LIMA, C.A.R.M.; CHIPPARI-GOMES, A.R.; LOPES, N.P.; URBINATI, E.C. 2003c Effect of fish density during transportation on stress and mortality of juvenile tambaqui Colossoma macropomum. Journal of the World Aquaculture
Society, 34(1): 76-84.
HOSEINI, S.M.; TORT, L.; RAJABIESTERABADI, M.H.A.H.; ABOLHASANI, M.H. 2016 Physiological, ionoregulatory, metabolic and imune responses of Persian sturgeon, Acipen serpersicus (Borodin, 1897) to stress. Aquaculture Research, 47(12): 3729-3739.
HOSHIBA, M.A.; GONí"¡ALVES, F.D.; URBINATI, E.C. 2009 Respostas fisiológicas de estresse no
matrinxã (Brycon amazonicus) após exercício físico intenso durante a captura. Acta Amazonica,
39(2): 445-452.
IBGE í INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA. 2014 Produção da Pecuária
Municipal. Rio de Janeiro, 42: 1-39.
INOUE, L.A.K.A.; MORAES, G. 2007 Óleo de Cravo: um anestésico alternativo para o manejo de peixes. Embrapa Amazônia Ocidental, Manaus, 24p.
MARTINS, M.L.; MORAES, F.R.; FUJIMOTO, R.Y.; NOMURA, D.T.; FENERICK JUNIOR, J. 2002
Respostas do híbrido tambacu (Piaractus mesopotamicus Holmberg, 1887 macho x Colossoma macropomum Cuvier, 1818 fêmea) a estímulos simples ou consecutivos de captura.
Boletim do Instituto de Pesca, 28(2): 195-204.
McDONALD, G.; MILLIGAN, C.L. 1997 Ionic, osmotic and acid-base regulation in stress. In: IWAMA, G.W.; PICKERING, A.D.; SUMPTER, J.P.; SCHRECK, C.B. Fish stress and health in aquaculture. University Press, Cambridge, p.119-144.
MELO, D.C.; OLIVEIRA, D.A.A.; MELO, M.M.; JíÅ¡NIOR, D.V.; TEIXEIRA, E.A.; GUIMARíES, S.R. 2009 Perfil protéico de tilápia nilótica chitralada (Oreochromis niloticus), submetida ao estresse crônico por hipóxia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(5): 1183-1190
MILLIGAN, C.L.; WOOD, C.M. 1987 Regulation of blood oxygen transport and red cell pHi after exhaustive activity in rainbow trout (Salmo gairdneri) and starry flounder (Platichthys stellatus).
Journal of the Experimental Biology, 133: 263-282.
MORGAN, J.D.; IWAMA, G.K. 1997 Measurements of stressed states in the field. In: IWAMA, G.K.; PICKERING, A.D.; SUMPTER, J.P.; SCHRECK, C.B. Fish stress and Health in Aquaculture. Society for Experimental Biology. Cambridge University Press, Cambridge, 62: 247-270.
MORO, G.V.; TORATI, L.S.; LUIZ, D.B.; MATOS, F.T. 2013 Monitoramento e manejo da qualidade da água em pisciculturas. In: RODRIGUES, A.P.O.; LIMA, A.F.; ALVES, A.L.; ROSA, D.K.; TORATI, L.S.; SANTOS, V.R.V. Piscicultura de água doce: multiplicando conhecimentos. Embrapa Pesca e Aquicultura, Palmas, p.141-169.
OLIVEIRA-JUNIOR, A.A.; TAVARES-DIAS, M.; MARCON, J.L. 2008 Biochemical and hematological reference ranges for Amazon freshwater turtle, Podocnemis expansa (Reptilia: Pelomedusidae), with morphologic assessment of blood cells. Research in Veterinary Science,
86(1): 146-151.
SILVA-ACUí"˜A, A.; GUEVARA, M. 2002 Evaluación de dos dietas comerciales sobre el crecimiento
del híbrido de Colossoma macropomum x Piaractus brachypomus. Zootecnia Tropical, 20(4): 449-459.
TAVARES-DIAS, M.; SANDRIM, E.F.S.; MORAES, F.R.; CARNEIRO, P.C.F. 2001 Physiological responses of "tambaqui†Colossoma macropomum (Characidae) to acute stress. Boletim do Instituto de Pesca, 27(1): 43-48.
TAKAHASHI, L.S.; ABREU, J.S.; BILLER, J.D.; URBINATI, E.C. 2006. Efeito do ambiente póstransporte na recuperação dos indicadores de estresse de pacus juvenis, Piaractus mesopotamicus. Acta Scientiarum Animal Sciences, 28(4): 469-475.
TUKEY, J.W. 1953 The problem of multiple comparisons. Mimeographs, Princeton University,
Princeton, N.J. 396p.
URBINATI, E.C.; ABREU, J.S.; CAMARGO, A.C.S.; PARRA, M.A.L. 2004 Loading and transport
stress of juvenile matrinxã (Brycon cephalus, Characidae) at various densities. Aquaculture,
229(1-4): 389-400.
URBINATI, E.C.; ZANUZZO, F.S.; CRISCUOLOURBINATI, E. 2014 Estratégias para redução do
estresse e enfermidades na piscicultura. In: SOUZA, A.T.S.; LIZAMA, M.L.A.P.; TAKEMOTO,
R.M. Patologia e sanidade em ambientes aquáticos. Massoni Gráfica e Editora, Maringá, p.185-203.
WENDELAAR BONGA, S.E. 1997 The stress response in fish. Physiological Reviews, 77(3): 591-625.
WOODY, C.A.; NELSON, J.; RAMSTAD, K. 2002 Clove oil as an anaesthetic for adult sockeye
salmon: field trails. Journal of Fish Biology, 60(2): 340-347.
different levels of vitamin C and submitted to air exposure. Acta Amazonica, 36(4): 519-524.
ABREU, J.S.; TAKAHASHI, L.S.; HOSHIBA, M.A.; URBINATI, E.C. 2009 Biological indicators of
stress in pacu (Piaractus mesopotamicus) after capture. Brazilian Journal of Biology, 69(2): 415-421.
ARAíÅ¡JO-LIMA, C.A.R.M.; GOMES, L.C. 2010 Tambaqui (Colossoma macropomum). In: BALDISSEROTTO, B.; GOMES, L.C. Espécies nativas para piscicultura no Brasil. Ed. Santa
Maria: UFSM, p.175-204.
BARTON, B.A. 2002 Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42(3): 517-525.
BARTON, B.A.; SCHRECK, C.B.; BARTON, L.D. 1987 Effects of chronic cortisol administration
and daily acute stress on growth, physiological conditions, and stress responses in juvenile
rainbow trout. Diseases of Aquatic Organisms, 2: 173-185.
BOSISIO, F.; REZENDE, K.F.O.; BARBIERI, E. 2017 Alterations in the hematological parameters of
Juvenile Nile Tilapia (Oreochromis niloticus) submitted to different salinities. Pan-American
Journal of Aquatic Sciences, 12(2): 146-154.
CHAGAS, E.C.; BOIJINK, C.L.; INOUE, L.A.K.A.; ARAíÅ¡JO, L.D.; GOMES, L.C.; MORAES, F.R.
2012 Tambaqui responses to stress due to transport after feeding with β-glucan supplemented diets. Biotemas, 25(4): 221-227.
EMERSON, K.; RUSSO, R.C.; LUND, R.E.; THURSTON, R.V. 1975 Aqueous ammonia equilibrium calculations: Effect of pH and temperature. Journal of the Fisheries Research
Board of Canada, 32(12): 2379-2383.
FAGUNDES, M.; URBINATI, E.C. 2008 Stress in pintado (Pseudoplatystoma corruscans) during
farming procedures. Aquaculture, 276(1-4): 112-119.
FEITOSA, K.C.O.; POVH, J.A.; ABREU, J.S. 2013 Physiological responses of pacu (Piaractus
mesopotamicus) treated with homeopathic product and submitted to transport stress.
Homeopathy, 102(4): 268-273.
GAMPERL, A.K.; VIJAYAN, M.M.; BOUTILIER, R.G. 1994 Experimental control of stress hormone
levels in fishes: techniques and applications. Reviews in Fish Biology and Fisheries, 4(2): 215-255.
GOMES, L.C.; ARAUJO-LIMA, C.A.R.M.; ROUBACH,R.; URBINATI, E.C. 2003a Avaliação dos efeitos
da adição de sal e da densidade no transporte de tambaqui. Pesquisa Agropecuária Brasileira, 38(2): 283-290.
GOMES, L.C.; ROUBACH, R.; CAVERO, B.A.S.; PEREIRA-FILHO, M.; URBINATI, E.C. 2003b
Transport of pirarucu Arapaima gigas juveniles in plastic bag. Acta Amazonica, 33(4): 637-642.
GOMES, L.C.; ROUBACH, R.; ARAUJO -LIMA, C.A.R.M.; CHIPPARI-GOMES, A.R.; LOPES, N.P.; URBINATI, E.C. 2003c Effect of fish density during transportation on stress and mortality of juvenile tambaqui Colossoma macropomum. Journal of the World Aquaculture
Society, 34(1): 76-84.
HOSEINI, S.M.; TORT, L.; RAJABIESTERABADI, M.H.A.H.; ABOLHASANI, M.H. 2016 Physiological, ionoregulatory, metabolic and imune responses of Persian sturgeon, Acipen serpersicus (Borodin, 1897) to stress. Aquaculture Research, 47(12): 3729-3739.
HOSHIBA, M.A.; GONí"¡ALVES, F.D.; URBINATI, E.C. 2009 Respostas fisiológicas de estresse no
matrinxã (Brycon amazonicus) após exercício físico intenso durante a captura. Acta Amazonica,
39(2): 445-452.
IBGE í INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA. 2014 Produção da Pecuária
Municipal. Rio de Janeiro, 42: 1-39.
INOUE, L.A.K.A.; MORAES, G. 2007 Óleo de Cravo: um anestésico alternativo para o manejo de peixes. Embrapa Amazônia Ocidental, Manaus, 24p.
MARTINS, M.L.; MORAES, F.R.; FUJIMOTO, R.Y.; NOMURA, D.T.; FENERICK JUNIOR, J. 2002
Respostas do híbrido tambacu (Piaractus mesopotamicus Holmberg, 1887 macho x Colossoma macropomum Cuvier, 1818 fêmea) a estímulos simples ou consecutivos de captura.
Boletim do Instituto de Pesca, 28(2): 195-204.
McDONALD, G.; MILLIGAN, C.L. 1997 Ionic, osmotic and acid-base regulation in stress. In: IWAMA, G.W.; PICKERING, A.D.; SUMPTER, J.P.; SCHRECK, C.B. Fish stress and health in aquaculture. University Press, Cambridge, p.119-144.
MELO, D.C.; OLIVEIRA, D.A.A.; MELO, M.M.; JíÅ¡NIOR, D.V.; TEIXEIRA, E.A.; GUIMARíES, S.R. 2009 Perfil protéico de tilápia nilótica chitralada (Oreochromis niloticus), submetida ao estresse crônico por hipóxia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(5): 1183-1190
MILLIGAN, C.L.; WOOD, C.M. 1987 Regulation of blood oxygen transport and red cell pHi after exhaustive activity in rainbow trout (Salmo gairdneri) and starry flounder (Platichthys stellatus).
Journal of the Experimental Biology, 133: 263-282.
MORGAN, J.D.; IWAMA, G.K. 1997 Measurements of stressed states in the field. In: IWAMA, G.K.; PICKERING, A.D.; SUMPTER, J.P.; SCHRECK, C.B. Fish stress and Health in Aquaculture. Society for Experimental Biology. Cambridge University Press, Cambridge, 62: 247-270.
MORO, G.V.; TORATI, L.S.; LUIZ, D.B.; MATOS, F.T. 2013 Monitoramento e manejo da qualidade da água em pisciculturas. In: RODRIGUES, A.P.O.; LIMA, A.F.; ALVES, A.L.; ROSA, D.K.; TORATI, L.S.; SANTOS, V.R.V. Piscicultura de água doce: multiplicando conhecimentos. Embrapa Pesca e Aquicultura, Palmas, p.141-169.
OLIVEIRA-JUNIOR, A.A.; TAVARES-DIAS, M.; MARCON, J.L. 2008 Biochemical and hematological reference ranges for Amazon freshwater turtle, Podocnemis expansa (Reptilia: Pelomedusidae), with morphologic assessment of blood cells. Research in Veterinary Science,
86(1): 146-151.
SILVA-ACUí"˜A, A.; GUEVARA, M. 2002 Evaluación de dos dietas comerciales sobre el crecimiento
del híbrido de Colossoma macropomum x Piaractus brachypomus. Zootecnia Tropical, 20(4): 449-459.
TAVARES-DIAS, M.; SANDRIM, E.F.S.; MORAES, F.R.; CARNEIRO, P.C.F. 2001 Physiological responses of "tambaqui†Colossoma macropomum (Characidae) to acute stress. Boletim do Instituto de Pesca, 27(1): 43-48.
TAKAHASHI, L.S.; ABREU, J.S.; BILLER, J.D.; URBINATI, E.C. 2006. Efeito do ambiente póstransporte na recuperação dos indicadores de estresse de pacus juvenis, Piaractus mesopotamicus. Acta Scientiarum Animal Sciences, 28(4): 469-475.
TUKEY, J.W. 1953 The problem of multiple comparisons. Mimeographs, Princeton University,
Princeton, N.J. 396p.
URBINATI, E.C.; ABREU, J.S.; CAMARGO, A.C.S.; PARRA, M.A.L. 2004 Loading and transport
stress of juvenile matrinxã (Brycon cephalus, Characidae) at various densities. Aquaculture,
229(1-4): 389-400.
URBINATI, E.C.; ZANUZZO, F.S.; CRISCUOLOURBINATI, E. 2014 Estratégias para redução do
estresse e enfermidades na piscicultura. In: SOUZA, A.T.S.; LIZAMA, M.L.A.P.; TAKEMOTO,
R.M. Patologia e sanidade em ambientes aquáticos. Massoni Gráfica e Editora, Maringá, p.185-203.
WENDELAAR BONGA, S.E. 1997 The stress response in fish. Physiological Reviews, 77(3): 591-625.
WOODY, C.A.; NELSON, J.; RAMSTAD, K. 2002 Clove oil as an anaesthetic for adult sockeye
salmon: field trails. Journal of Fish Biology, 60(2): 340-347.
Downloads
Published
2017-12-14
Issue
Section
Scientific Article
