Hydroalcoholic extract of jabuticaba peel in the diet of betta fish





ornamental fish, antioxidant, coloring


The objective of this study was to evaluate the effects of hydroalcoholic jabuticaba bark on the diets of female blue Betta splendens in relation to the level of digestive enzymes, liver metabolism, and antioxidant activity. The sample constituted 150 individuals subdivided into five groups in triplicate, totaling 10 fish per 20 L experimental unit. Commercial diets (40.88% CP and 4374.8 Kcal kg-1) were added to jabuticaba bark (EJB) hydroalcoholic extract concentrations of 0.5, 1.0, 1.5, and 2.0 g .kg-1. The rations were provided twice a day, until apparent satiety, for 21 days. Fish mortality was not observed during the trial. Growth indices did not show any significant differences, apart from feed conversion. The use of the extract promoted an increase in the luminosity of the fish; however, there was no statistical difference in chromaticity a * and b *. A decrease in the activity of Superoxide dismutase (SOD) on the skin of fish fed diets of 1.5 and 2.0g.kg-1 EJB was observed in comparison to the other diets. There was no change in CAT activity. It is concluded that the use of 2 g kg-1 EJB has an antioxidant effect that reflects the greater luminosity of blue female Betta splendens.


Amar, E.C.; Kiron, V.; Satoh, S.; Watanabe, T. 2001. Influence of various dietary synthetic carotenoids on bio‐defence mechanisms in rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 32(Suppl. 1): 162-173. https://doi.org/10.1046/j.1355-557x.2001.00051.x

AOAC – Association of Official Analytical Chemists. 2000. Official methods of analysis. Washington, D.C.: Association of Official Analytical Chemists.

Awad, E.; Awaad, A. 2017. Role of medicinal plants on growth performance and immune status in fish. Fish & Shellfish Immunology, 67: 40-54. https://doi.org/10.1016/j.fsi.2017.05.034

Aydın, B.; Barbas, L.A.L. 2020. Sedative and anesthetic properties of essential oils and their active compounds in fish: a review. Aquaculture, 520: 734999. https://doi.org/10.1016/j.aquaculture.2020.734999

Aydın, B.; Yılmaz, S.; Gümüş, E. 2017. The effect of distiller’s dried grains with solubles on carcass composition, fatty acid composition, skin and fillet coloration of rainbow trout (Oncorhynchus mykiss). Israeli Journal of Aquaculture, 69: 1-10. https://doi.org/10.46989/001c.20868

Bendokas, V.; Skemiene, K.; Trumbeckaite, S.; Stanys, V.; Passamonti, S.; Borutaite, V.; Liobikas, J. 2020. Anthocyanins: from plant pigments to health benefits at mitochondrial level. Critical Reviews in Food Science and Nutrition, 60(19): 3352-3365. https://doi.org/10.1080/10408398.2019.1687421

Benvenutti, L.; Zielinski, A.A.F.; Ferreira, S.R.S. 2021. Jaboticaba (Myrtaceaecauliflora) fruit and its by-products: Alternative sources for new foods and functional components. Trends in Food Science & Technology, 112: 118-136. https://doi.org/10.1016/j.tifs.2021.03.044.

Beutler, E. 1984. Red cell metabolism: a manual of biochemical methods. 3 e. Orlando: Grune & Stratton, 188 p.

Bordenave, M.L.; Huc, A.Y. 1995. The Cretaceous source rocks in the Zagros foothills of Iran. Revue de L'institut Français duPetrole, 50(6): 727-752. https://doi.org/10.2516/ogst:1995044

Bridle, P.; Timberlake, C.F. 1997. Anthocyanins as natural food colours—selected aspects. Food Chemistry, 58(1-2): 103-109. https://doi.org/10.1016/S0308-8146(96)00222-1

Carmo-Ota, E.; Honorato, C.A.; Heredia-Vieira, S.C.; Flores-Quintana, C.I.; Castro Silva, T.S.; Inoue, L.A.K.A.; Cardoso, C.A.L. 2019. Hepatic and gastroprotective activity of Serjania marginata leaf aqueous extract in Nile tilapia (Oreochromis niloticus). Fish Physiology and Biochemistry, 45(3): 1051-1065. https://doi.org/10.1007/s10695-019-00622-9

Celli, G.B.; Brooks, M.S.L. 2017. Impact of extraction and processing conditions on betalains and comparison of properties with anthocyanins—A current review. Food Research International, 100(Part 3): 501-509. https://doi.org/10.1016/j.foodres.2016.08.034

Charron, C.S.; Kurilich, A.C.; Clevidence, B.A.; Simon, P.W.; Harrison, D.J.; Britz, S.J., Baer, D.J.; Novotny, J.A. 2009. Bioavailability of anthocyanins from purple carrot juice: effects of acylation and plant matrix. Journal of Agricultural and Food Chemistry, 57(4): 1226-1230. https://doi.org/10.1021/jf802988s

Dethlefsen, M.W.; Hjermitslev, N.H.; Frosch, S.; Nielsen, M.E. 2016. Effect of storage on oxidative quality and stability of extruded astaxanthin-coated fish feed pellets. Animal Feed Science and Technology, 221(Part A): 157-166. https://doi.org/10.1016/j.anifeedsci.2016.08.007

Dorce, L.S.; Mendonça, W.C.B.; Siqueira, M.S.; Santos, R.F.B.; Sousa, R.M.; Ziemniczak, H.M.; Honorato, C.A. 2020. Atividade das enzimas digestivas frente a restrição alimentar de peixes ornamentais. Agrarian, 13(47): 107-113. https://doi.org/10.30612/agrarian.v13i47.9912

Eaton, L.; Clezy, K.; Snellgrove, D.; Sloman, K. 2016. The behavioural effects of supplementing diets with synthetic and naturally sourced astaxanthin in an ornamental fish (Puntius titteya). Applied Animal Behaviour Science, 182: 94-100. https://doi.org/10.1016/j.applanim.2016.06.007

Ekin, S.; Dogan, M.; Gok, F.; Karakus, Y. 2018. Assessment of antioxidant enzymes, total sialic acid, lipid bound sialic acid, vitamins and selected amino acids in children with phenylketonuria. Pediatric Research, 84(6): 821-828. https://doi.org/10.1038/s41390-018-0137-2

Faria, G.S.; Jardim, F.B.B.; Silva, A.C.; Costa, L.L.; Abdalla, D.R. 2016. Caracterização química da casca de jabuticaba (Myrciaria jabuticaba) liofilizada e sua aplicação em leite fermentado potencialmente simbiótico. Jornal de Ciências Biomédicas e Saúde, 2(1): 2.

Ferreira, A.E.; Ferreira, B.S.; Lages, M.M.B.; Rodrigues, V.A.F.; Pinto, N.A.V.D. 2012. Produção, caracterização e utilização da farinha de casca de jabuticaba em biscoitos tipo cookie. Brazilian Journal of Food & Nutrition, 23(4): 603-607.

Freidig, A.K.; Goldman, I.L. 2014. Geosmin (2β, 6α-dimethylbicyclo [4.4. 0] decan-1β-ol) production associated with Beta vulgaris ssp. vulgaris is cultivar specific. Journal of Agricultural and Food Chemistry, 62(9): 2031-2036. https://doi.org/10.1021/jf4047336

Fries, E.M.; Bittarello, A.C.; Zaminhan, M.; Signor, A.; Feiden, A.; Boscolo, W.R. 2014. Urucum em dietas para alevinos de kinguios Carassiusauratus: Desempenho produtivo e pigmentação da pele. Semina: Ciências Agrárias, 35(6): 3401-3413. https://doi.org/10.5433/1679-0359.2014v35n6p3401

Gomes, L.C.; Chippari‐Gomes, A.R.; Lopes, N.P.; Roubach, R.; Araujo‐Lima, C.A. 2007. Efficacy of benzocaine as an anesthetic in juvenile tambaqui Colossoma macropomum. Journal of the World Aquaculture Society, 32(4): 426-431. https://doi.org/10.1111/j.1749-7345.2001.tb00470.x

Goodwin, T.W. 1954. Carotenoids, their comparative biochemistry. New York: Chemical Pub. Co, 356 p.

Gouveia, L.; Rema, P.; Pereira, O.; Empis, J. 2003. Colouring ornamental fish (Cyprinus carpio and Carassius auratus) with microalgal biomass. Aquaculture Nutrition, 9(2): 123-129. https://doi.org/10.1046/j.1365-2095.2003.00233.x

Guimarães, W.; Alves, M.I.R.; Antoniosi-Filho, N.R. 2012. Antocianinas em extratos vegetais: aplicação em titulação ácido-base e identificação via cromatografia líquida/espectrometria de massas. Química Nova, 35(8): 1673-1679. https://doi.org/10.1590/S0100-40422012000800030

Gumus, C.E.; Decker, E.A.; McClements, D.J. 2017. Impact of legume protein type and location on lipid oxidation in fish oil-in-water emulsions: Lentil, pea, and faba bean proteins. Food Research International, 100(Pt. 2): 175-185. https://doi.org/10.1016/j.foodres.2017.08.029

Hunt, R.W.G. 1977. The specification of colour appearance: I. Concepts and terms. Color Research and Application, 2(2): 55-68. https://doi.org/10.1002/col.5080020202

IBGE – Instituto Brasileiro de Geografia e Estatística. População de animais de estimação no Brasil. [online] URL: <http://www.agricultura.gov.br/assuntos/camaras-setoriais-tematicas/documentos/camaras-tematicas/insumos-agropecuarios/anos-anteriores/ibge-populacao-de-animais-de-estimacao-no-brasil-2013-abinpet-79.pdf> Accessed: Mar. 14, 2013.

Jahromi, S.T.; Pourmozaffar, S.; Jahanbakhshi, A.; Rameshi, H.; Gozari, M.; Khodadadi, M; Sohrabipour, J.; Behzadi, S.; Barzkar, N.; Nahavandi, R.; Zahedi, M.R.; Moezzi, M. 2021. Effect of different levels of dietary Sargassum cristaefolium on growth performance, hematological parameters, histological structure of hepatopancreas and intestinal microbiota of Litopenaeus vannamei. Aquaculture, 533: 736130. https://doi.org/10.1016/j.aquaculture.2020.736130

Kesbiç, O.S. 2019. Effects of juniper berry oil on growth performance and blood parameters in common carp (Cyprinus carpio). Aquaculture Research, 50(1): 342-349. https://doi.org/10.1111/are.13908

Kruger, N.J. 2009. The bradford method for protein quantitation. In: Walker, J. (ed.) The protein protocols handbook. Totowa: Humana Press, 1146 p. p. 19-20.

Lees, D.H.; Francis, F.J. 1972. Standardization of pigment analyses in cranberries. HortScience, 7(1): 83-84.

Leite-Legatti, A.V.; Batista, Â.G.; Dragano, N.R.V.; Marques, A.C.; Malta, L.G.; Riccio, M.F.; Eberlinb, M.N.; Machado, A.R.T.; Carvalho-Silva, L.B.; Ruiz, A.L.T.G.; Carvalho, J.E.; Pastore, G.M.; Maróstica Júnior, M.R. 2012. Jaboticaba peel: Antioxidant compounds, antiproliferative and antimutagenic activities. Food Research International, 49(1): 596-603. https://doi.org/10.1016/j.foodres.2012.07.044

Li, M.; Rahman, M.M.; Wu, B.; Lin, Y.C. 2017. Effects of dietary canthaxanthin on growth and body colour of blood parrot cichlid Amphilophus citrinellus x Paraneetroplus synspilus. Aquaculture International, 25: 705-713. https://doi.org/10.1007/s10499-016-0068-z

Lopes, T.; Xavier, M.; Quadri, M.G.; Quadri, M. 2007. Antocianinas: uma breve revisão das características estruturais e da estabilidade. Current Agricultural Science and Technology, 13(3): 291-297. https://doi.org/10.18539/cast.v13i3.1375

Marquetti, C.; Santos, T.B.; Kaipers, K.F.C.; Böger, B.R.; Tonial, I.B.; Wagner-Junior, A.; Lucchetta, l.; Prado, N.V. 2018. Jaboticaba skin flour: analysis and sustainable alternative source to incorporate bioactive compounds and increase the nutritional value of cookies. Food Science and Technology, 38(4): 629-638. https://doi.org/10.1590/fst.06717

Mazza, G.; Miniati, E. 1993. Anthocyanins in fruits, vegetables, and grains. Boca Raton: CRC Press, 3684 p. https://doi.org/10.1201/9781351069700

Menga, F.; Swyngedouw, E. 2018. Water, technology and the nation-state. Routledge, 242 p. https://doi.org/10.4324/9781315192321

Mirzaee, S.; Sabani, A.; Rezaee, S.; Hosseinzadeh, M. 2012. The effect of synthetic and natural pigments on the color of guppy fish Poecilia reticulata. Global Veterinaria, 9(2): 171-174. https://doi.org/10.5829/idosi.gv.2012.9.2.63200

Morais, F.A.; Fernandes, J.B.; Tavares, L.H. 2015. Diets supplemented with microalgal biomass: effects on growth, survival and colouration of ornamental fish Hyphessobrycon eques. Aquaculture Research, 47(10): 3061-3069. https://doi.org/10.1111/are.12756

Morales, P.; Barros, L.; Dias, M.I.; Santos-Buelga, C.; Ferreira, I.C.; Asquieri, E.R.; Berrios, J.D.J. 2016. Non-fermented and fermented jabuticaba (Myrciariacauliflora Mart.) pomaces as valuable sources of functional ingredients. Food Chemistry, 208: 220-227. https://doi.org/10.1016/j.foodchem.2016.04.011

Nunes, K.C.; Eyng, C.; Pintro, P.T.; Garcia, R.G.; Murakami, A.E.; Vital, A.C.; Nunes, R.V.; Nesello, P.O. 2019. Dietary inclusion of dehydrated bocaiuva pulp increases the antioxidant potential of quail eggs. Journal of Animal Physiology and Animal Nutrition, 103(1): 64-71. https://doi.org/10.1111/jpn.13003

Perez, C.P.; Lores, M.; Velando, A. 2008. Availability of nonpigmentary antioxidante affects red coloration in gulls. Behavioral Ecology, 19(5): 967-973. https://doi.org/10.1093/beheco/arn053

Porto, E.L.N.; Lima, F.F.; Sousa, R.M.; Babhara, D.G.A.; Mendonça, W.C.B.; Honorato, C.A. 2020a. Schinus terebinthifolius raddi pepper oil used as an addictive in Hyphessobrycon equesteindachner fish diet. Society and Development, 9(6): e192963118. https://doi.org/10.33448/rsd-v9i6.3118

Porto, E.L.N.; Lima, F.F.; Sousa, R.M.; Mendonça, W.C.B.; Honorato, C.A. 2020b. The Hyphessobrycon eques (Steindachner, 1882) coloration influenced by the addition of Attalea Phalerata Mart. ex spreng crude oil. Society and Development, 9(3): e97932578. https://doi.org/10.33448/rsd-v9i3.2578

Reitman, S.; Frankel, S. 1957. A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology, 28(1): 56-63. https://doi.org/10.1093/ajcp/28.1.56

Rezende, F.P.; Vidal Júnior, M.V.; Andrade, D.R.; Mendonça, P.P. 2012. Characterization of a new methodology based on the intensity of skin staining of ornamental fish with applications in nutrition. Journal of Agricultural Science and Technology B, 2(5B): 606-613.

Sales, J.; Janssens, G.P.J.J. 2003. Nutrient requirements of ornamental fish. Aquatic Living Resources, 16(6): 533-540. https://doi.org/10.1016/j.aquliv.2003.06.001

Sato, A.C.K.; Cunha, R.L. 2007. Influence of temperature on the rheological behavior of jaboticaba pulp. Ciência e Tecnologia de Alimentos, 27(4): 890-896. https://doi.org/10.1590/S0101-20612007000400033

Silva, M.C.; Souza, V.B.; Thomazini, M.; Silva, E.R.; Smaniotto, T.; Carvalho, R.A.; Genovese, M.I.; Favaro-Trindade, C.S. 2014. Use of the jabuticaba (Myrciariacauliflora) depulping residue to produce a natural pigment powder with functional properties. LWT - Food Science and Technology, 55(1): 203-209. https://doi.org/10.1016/j.lwt.2013.08.026

Singh, R.K.; Kumar, R.; Singh, D.P. 2016. Graphene oxide: strategies for synthesis, reduction and frontier applications. RSC Advances, 6(69): 64993-65011. https://doi.org/10.1039/C6RA07626B

Souza, K.C. 2019. Influência da temperatura da água no desempenho reprodutivo e diferenciação sexual do peixe Bettasplendens. 60. (Masters Dissertation. Universidade Federal de Minas Gerais). Available at: <https://repositorio.ufmg.br/bitstream/1843/31569/1/Dissertação%20Kenia%20%282%29.pdf> Accessed: Mar. 22, 2021.

Wang, C.Y.; Li, Z.B.; Sun, Y.Z.; Chen, Q.; Li, W.J.; Huang, Y.C.; Lu, J. 2018. Effects of Chinese herbal medicines mixture on growth performance digestive enzyme activity immune response of juvenile Japanese seabass, Lateolabrax japonicus. Aquaculture Nutrition, 24(2): 683-693. https://doi.org/10.1111/anu.12597

Xu, J.; Kuhnt, W.; Holbourn, A.; Andersen, N.; Bartoli, G. 2006. Changes in the vertical profile of the Indonesian Throughflow during Termination II: Evidence from the Timor Sea. Paleoceanography, 21(4). https://doi.org/10.1029/2006PA001278

Yi, X.; Li, J.; Xu, W.; Zhou, H.; Smith, A.A.; Zhang, W.; Mai, K. 2015. Shrimp shell meal in diets for large yellow croaker Larimichthys croceus: Effects on growth, body composition, skin coloration and anti-oxidative capacity. Aquaculture, 441: 45-50. https://doi.org/10.1016/j.aquaculture.2015.01.030

Yilmaz, E. 2020. Effect of dietary carob (Ceratonia siliqua) syrup on blood parameters, gene expression responses and ammonia resistance in tilapia (Oreochromis niloticus). Aquaculture Research, 51(5): 1903-1912. https://doi.org/10.1111/are.14540

Yousefi, M.; Vatnikov, Y.A.; Kulikov, E.V.; Ahmadifar, E.; Mirghaed, A.T.; Hoseinifar, S.H.; Doan, H.V. 2021. Effects of dietary Hibiscus sabdariffa supplementation on biochemical responses and inflammatory-related genes expression of rainbow trout, Oncorhynchus mykiss, to ammonia toxicity. Aquaculture, 533: 736095. https://doi.org/10.1016/j.aquaculture.2020.736095






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