Steroid profile and ovarian gene expression in Genyatremus luteus (Teleostei, Perciformes: Haemulidae) during sexual maturation

Diego Luiz dos Santos Ribeiro; Larissa Sarmento dos Santos Ribeiro; João Mandu da Silva; Katherine Saldanha Noleto; Nancyleni Pinto Chaves Bezerra; Fernando Almeida Souza; Alcina Vieira de Carvalho-Neta; José Ribamar de Souza Torres Junior

doi:10.20950/1678-2305/bip.2023.49.e717

Authors

Diego Luiz dos Santos Ribeiro Universidade Federal do Maranhão, Rede de Biodiversidade e Biotecnologia da Amazônia Legal – São Luís (MA), Brazil. https://orcid.org/0000-0003-3863-4933
Larissa Sarmento dos Santos Ribeiro Universidade Estadual do Maranhão, Departamento de Patologia – São Luís (MA), Brazil. https://orcid.org/0000-0001-8237-1988
João Mandu da Silva Universidade Estadual do Maranhão, Curso de Engenharia de Pesca – São Luís (MA), Brazil. https://orcid.org/0000-0003-0139-8885
Katherine Saldanha Noleto Universidade Federal do Maranhão, Rede de Biodiversidade e Biotecnologia da Amazônia Legal – São Luís (MA), Brazil. https://orcid.org/0000-0002-5326-5737
Nancyleni Pinto Chaves Bezerra Universidade Estadual do Maranhão, Departamento de Patologia – São Luís (MA), Brazil. https://orcid.org/0000-0003-3970-7524
Fernando Almeida Souza Instituto Oswaldo Cruz, Laboratório de Imunomodulação e Protozoologia – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0003-0047-6159
Alcina Vieira de Carvalho-Neta Universidade Estadual do Maranhão, Departamento de Química e Biologia – São Luís (MA), Brazil. https://orcid.org/0000-0001-7132-5265
José Ribamar de Souza Torres Junior Universidade Federal do Maranhão, Departamento de Oceanografia e Limnologia – São Luís (MA), Brazil. https://orcid.org/0000-0002-6356-6517

DOI:

https://doi.org/10.20950/1678-2305/bip.2023.49.e717

Keywords:

Gonadotropin receptor, Estradiol, Gonads, Reproduction, Grunts

Abstract

The aim of the present study was to assess histological features, sex steroid profile, and ovarian gene expression throughout the ovarian maturation of Genyatremus luteus in Brazilian Coastal Equatorial Amazon. Specimens were categorized into different gonadal maturation stages–immature (n = 7), maturing (n = 7), mature (n = 7), and spawned (n = 7)–based on histological features of ovarian follicles and on oocyte development. Plasma E2, 11-KT and 17α-OHP levels were measured through enzyme-linked immunosorbent assays. The gene expression of estrogen (er) and LH (lhr) receptors in the ovary was analyzed through real-time polymerase chain reaction. The highest plasma E2 concentration was found in mature specimens (P< 0.05). Plasma 17α-OHP concentrations have significantly increased from the immature to the final maturation stage (P< 0.05). Maturational reproductive stage did not have any effect on plasma 11-KT concentrations. Er expression in ovaries has significantly increased throughout ovarian maturation (P < 0.05), and it remained high at mature and spawned stages. Lhr expression showed similar trend throughout the maturation process, although its peak was observed at spawned stage (P< 0.05). In conclusion, the gene expression profile of er and lhr and plasma E2 and 17α-OHP concentrations are proportional to oocyte development; they play important role in regulating ovarian development and ovulation in G. luteus.

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