Perfil de esteroides e expressão gênica ovariana em Genyatremus luteus (Teleostei, Perciformes: Haemulidae) durante a maturação sexual

Autores

DOI:

https://doi.org/10.20950/1678-2305/bip.2023.49.e717

Palavras-chave:

Receptor de gonadotrofina, Estradiol, Gônadas, Reprodução, Roncador

Resumo

O objetivo deste estudo foi avaliar as características histológicas, o perfil de esteroides sexuais e a expressão gênica ovariana ao longo do desenvolvimento da maturação ovariana de Genyatremus luteus na Amazônia equatorial costeira brasileira. Os espécimes foram categorizados em diferentes estágios de maturação gonadal – imaturo (n = 7), em maturação (n = 7), maduro (n = 7) e desovado (n = 7) –, com base nas características histológicas dos folículos ovarianos e no desenvolvimento do oócito. Os níveis plasmáticos de E2, 11-KT e 17α-OHP foram medidos por meio de ensaio de imunoabsorção enzimática. A expressão gênica dos receptores de estrogênio (er) e LH (lhr) no ovário foi analisada por reação em cadeia da polimerase em tempo real. A maior concentração plasmática de E2 foi encontrada em espécimes maduros (P <0,05). As concentrações plasmáticas de 17α-OHP aumentaram significativamente desde o estágio imaturo até o estágio final de maturação (P < 0,05). O estágio reprodutivo maturacional não teve nenhum efeito nas concentrações plasmáticas de 11-KT. A expressão de er nos ovários aumentou significativamente ao longo da maturação ovariana (P < 0,05) e permaneceu alta nos estágios de maturação e desova. A expressão de lhr apresentou tendência semelhante ao longo do processo de maturação, embora tenha atingido seu pico na fase de desova (P < 0,05). Em conclusão, o perfil de expressão gênica de er e lhr e as concentrações plasmáticas de E2 e 17α-OHP são proporcionais ao desenvolvimento oocitário e desempenham papel importante na regulação do desenvolvimento ovariano e ovulação em G. luteus.

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2023-12-01

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