Perfil de esteroides e expressão gênica ovariana em Genyatremus luteus (Teleostei, Perciformes: Haemulidae) durante a maturação sexual
DOI:
https://doi.org/10.20950/1678-2305/bip.2023.49.e717Palavras-chave:
Receptor de gonadotrofina, Estradiol, Gônadas, Reprodução, RoncadorResumo
O objetivo deste estudo foi avaliar as características histológicas, o perfil de esteroides sexuais e a expressão gênica ovariana ao longo do desenvolvimento da maturação ovariana de Genyatremus luteus na Amazônia equatorial costeira brasileira. Os espécimes foram categorizados em diferentes estágios de maturação gonadal – imaturo (n = 7), em maturação (n = 7), maduro (n = 7) e desovado (n = 7) –, com base nas características histológicas dos folículos ovarianos e no desenvolvimento do oócito. Os níveis plasmáticos de E2, 11-KT e 17α-OHP foram medidos por meio de ensaio de imunoabsorção enzimática. A expressão gênica dos receptores de estrogênio (er) e LH (lhr) no ovário foi analisada por reação em cadeia da polimerase em tempo real. A maior concentração plasmática de E2 foi encontrada em espécimes maduros (P <0,05). As concentrações plasmáticas de 17α-OHP aumentaram significativamente desde o estágio imaturo até o estágio final de maturação (P < 0,05). O estágio reprodutivo maturacional não teve nenhum efeito nas concentrações plasmáticas de 11-KT. A expressão de er nos ovários aumentou significativamente ao longo da maturação ovariana (P < 0,05) e permaneceu alta nos estágios de maturação e desova. A expressão de lhr apresentou tendência semelhante ao longo do processo de maturação, embora tenha atingido seu pico na fase de desova (P < 0,05). Em conclusão, o perfil de expressão gênica de er e lhr e as concentrações plasmáticas de E2 e 17α-OHP são proporcionais ao desenvolvimento oocitário e desempenham papel importante na regulação do desenvolvimento ovariano e ovulação em G. luteus.
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Copyright (c) 2023 Diego Luiz dos Santos Ribeiro, Larissa Sarmento dos Santos Ribeiro, João Mandu da Silva, Katherine Saldanha Noleto, Nancyleni Pinto Chaves Bezerra, Fernando Almeida Souza, Alcina Vieira de Carvalho-Neta, José Ribamar de Souza Torres Junior
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