Abnormal morphology of "curimbatá†Prochilodus scrofa ( Steindachner,1881 ) ( Osteichthyes, Characiformes, Prochilodontidae ) spermatozoa
Keywords:
curimbatá, Prochilodus scrofa, spermatozoa morphology, hormonal inductionAbstract
Semen was collected from 48 "curimbata" fish (Prochilodus scrofa) 3 years old, prior and post two i.m. injections of hCG, in order to verify possible abnormal morphology in spermatozoa. Under differential interference contrast microscopy, 384 samples of semen were examined, being 192 smears stained by Williams method and 192 wet preparations in 0.2% glutaraldeyde. Sperm motility was also evaluated under differential interference contrast microscopy. Statistical analyses showed high significant correlation (P<0.01) between sperm head abnormalities and time elapsed from hormonal treatment. The minor defects plus major ones resulted in 9.54% in average while sperm motility resulted in a mean value of 94.17%. Overall higher defects observed were bent tails and free sperm heads.
References
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BARTH, A. D. & OKO, R.J. 1989 Abnormal morphology of bovine spermatozoa. Ames, Iowa State University
Press, 285 p.
BILLARD, R. 1970 Ultrastructure comparée de spermatozoides de quelques poissons Téléosteens. -In: BACCETI, B., ed. Comparative spermatology. New York,Academic Press. p. 71-9.
BILLARD, R.; DUPON, J.; BARNABE, G. 1977 Diminution de la motilité et de la durée de conservation du sperme de Dicentrarchus labrax L. (poisson téléosteen) pendant la periode de spermiation. Aquaculture,Amsterdam, 11(4):363-7.
BILLARD, R. & TAKASHIMA, F. 1983 Resorption of spermatozoa in the sperm duct of rainbow trout during
the post-spawning period. Bull. Japan. Soc. Sci. Fish., 39(3):387-392.
BLOM, E. 1973 Ultrastrukturen af nogle spermiedefekter of for slag til et nyt klassificerings-system for tyrens
spermiogram. The ultrastructure of some characteristic sperm defect and a proposal for a new classification of bull spermiogram. Nord. Vet. Med., 25(7-8):383-91.
BORGES FILHO, O.F. 1987 Caracterização dos estádios de maturação e correlação com avaliações histoquímico-enzimáticas e ultraestruturais das células endócrinas testiculares, durante o ciclo reprodutivo de Prochilodus scrofa Steindachner, 1881. São Paulo,Universidade de São Paulo. 234 p. (Tese de Doutorado. Instituto de Biociências, USP).
GINZBURG, A.S. 1972 Fertilization in fishes and the problem of polyspermy. Springfield, Department of Commerce, National AM Tecnical Information Service, p. 87-359.
GWO, J.C.; KUROKURA, H.; HIRANO, R. 1993 Cryopreservation of spermatozoa from rainbow trout, common
carp and marine puffer. Nippon Suissan Gakkaishi,59(5): 777-82.
GRIER, H.J. 1985 Cellular organization of the testis and spermatogenesis in fishes. Amer. Zool., 21: 345-57.
KAVAMOTO, E. T.; SILVEIRA, W. F.; RIGOLINO, M. G.; CARVALHO FILHO, A. C. 1985 Avaliação macro e microscópica do sêmen da truta arco-íris, Salmo irideus Gibbons. B. Inst. Pesca, São Paulo, 12(3):73-81.
LAHNSTEINER, F.; PATZNER, R.A.; WEISMANN, T. 1993 The spermatic ducts of salmonid fishes (Salmonidae, Teleostei). Morphology, histochemistry and composition of the secretion. J. Fish Biol., 42 (1): 79-93.
LAHNSTEINER, F.; PATZNER, R.A.; WEISMANN, T. 1994 Testicular main ducts and spermatic ducts in some Cypinid fishes. I. Morphology, fine structure and histochemistry. J. Fish Biol., 44 (3): 459 - 67.
LAM, T.J. 1985 Applications of endocrinology to fish culture. Can. J. Fish Aquat. Sci., 39: 11-137.
NAGAHAMA, Y. 1983 The functional morphology of teleost gonads. In: HOAR, W.S.;RANDAL. D.J. Fish physiology. New York, Academic Press, v.9 p. 233-275.
ROMAGOSA, E.; NARAHARA, M.Y.; ANDRADE-TALMELLI, E. F. de; BRAGA, F. M. S. 1993 Mudanças morfológicas dos testículos de pacu, Piaractus mesopotamicus (HOLMBERG, 1887), em condições de confinamento . Revista UNIMAR, 15: 1-17.
SALISBURY, G. M. & VANDEMARK, N. L. 1964 Fisiologia de la reproducción y de la inseminación artificial de los bóvidos. Zaragoza, Acribia, 707 p.
SCOTT, A. P. & BAYNES, S. M. 1980 A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol. 17: 707 - 39.
SNEDECOR, G.W. & COCHRAN, W.G. 1980 Métodos estatísticos. México Continental, 7 ed., 703p.
WILLIAMS, W. W. 1920 Technique of collecting semen for laboratory examination with a review of several diseased bulls. Cornell Vet.,10: 87 - 94.
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2018-10-26
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