Análisis preliminares de la composición lipídica de las gónadas de bagre negro, Rhamdia quelen (Siluriformes; Quoy y Gaimard, 1824), de dos poblaciones en diferentes estaciones del aí±o
Keywords:
black catfish, Rhamdia quelen, lipid composition, gonadAbstract
Rhamdia quelen is a siluriform with an extensive geographical distribution. The species inhabits freshwater rivers and estuarine lagoons. The fatty acid composition of the fi sh gonadal tissues is the result of its diet. However, this may be affected by intrinsic or extrinsic factors. In this study, eight females of R. quelen were captured in two places: a freshwater river (SG) and a coastal lagoon (LR). In the river (SG), the capture was inside the reproductive period, and in the lagoon (LR), the capture was outside this period. Total lipids were determined gravimetrically and the fatty acids were separated by gas chromatography. Total lipid (SG=3.5%w.w.; LR=4.0%w.w.) do not show differences between the two populations. A total of 41 fatty acids were identifi ed, and among them the more relevant were: C16:0 (SG=22.7%; LR=21.4%), C18:1n9 (SG=11.5%; LR=11.7%), C20:4n6 (SG=11.1%; LR=12.5%) and C22:6n3 (SG=10.9%; LR=12.2%). Although the females belong to different ambients and season, no differences in the fatty acid composition were detected. However, studies with a greater number of samples are indispensable. A stability in the gonadal fatty acid profi le was recorded, independently of the fi sh habitat. Similar values were recorded for other species.
References
A U B R I O T , L . ; C O N D E , D . ; B O N I L L A , S . ;SOMMARUGA, R. 2004 Phosphate uptake behavior of natural phytoplankton during exposure to solar ultraviolet radiation in a shallow coastal lagoon. Marine Biology, 144:623-631.
BORLONGAN, I.G. y BENITEZ, L.V. 1992 Lipid and fatty acid composition of milkfi sh (Chanos chanos
Forsskal) grown in freshwater and seawater. Aquaculture, Amsterdam, 104: 79-89.
CHRISTIE, W.W. 1982 Lipid Analysis. Oxford:Pergamon Press. 207p.
DE SILVA, S.S; GUNASEKERA, R.M.; AUSTIN, C.M. 1997 Changes in the fatty acid profi les of hybrid red tilapia Oreochromis mossambicus x O. niloticus, subjected to short-term starvation, and comparison with changes in seawater raised fish.Aquaculture, Amsterdam, 153: 273-290.
DE SILVA, S.S.; GUNASEKERA, R.M.; AUSTIN, C.M.; ALLINSON, G. 1998 Habitat related variations in fatty acids of catadromus Galaxias maculatus. Aquatic Living Resources, 11(6): 379-385.
FOLCH, J.; LESS, S.; STANLEY, G.H.S. 1957 A simple method for isolation and purifi cation of total lipids
from animals tisues. J. Biol. Chem., 22: 497-509.
FRACALOSSI, D.M.; ZANIBONI FILHO, E.;MEURER, S. 2002 No rastro das espécies nativas. Panorama da Aq¸uicultura, 12: 43-49.
GARCÍA, D.J. 1995 Aspectos Biológicos del Bagre Negro con Enfasis en su Alimentacií"ºn. Montevideo. 66p. (Tesis
de Licenciatura. Facultad de Ciencias -UDELAR).
JEONG, B.Y.; MOON, S-K.; JEONG, S.; OHSHIMA, T.2000 Lipid classes and fatty acid compositions of wild and cultured sweet smelt Plecoglossus altivelis muscle and eggs in Korea. Fisheries Science, 66: 716-724.
JEONG, B-Y.; JEONG, S.; MOON, S-K.; OHSHIMA, T. 2002 Preferential accumulation of fatty acids in the testis and ovary of cultured and wild sweet smel Plecoglossus altivelis. Comp. Biochem. Physiol.(B), 131: 251-259.
LUCHINI, L. 1990 Manual para el cultivo del Bagre Sudamericano (Rhamdia sapo). Santiago de Chile: Organización de las Naciones Unidas para la Agricultura y la Alimentación. Oficina Regional para America Latina y el Caribe. 60p. RLAC/90/16-PES-20.
MEYER, G. y FRACALOSSI, D.M. 2004 Protein requirement of jundi· fingerlings, Rhamdia quelen, at two dietary energy concentrations. Aquaculture,Amsterdam, 240: 331-343.
OLSEN, Y. 1998 Lipids and essential fatty acids in aquatic food webs. In: ARTS, M.T y WAINMAN, B.C. (Ed.). Lipids in Freshwaters Ecosystems. New York: Springer-Verlag. p.168-199.
RINGUELET, R.A.; ARAMBURU, R.H.; DE ARAMBURU, A.A. 1967 Los peces Argentinos de Água Dulce. La Plata: Direccií"ºn de Impresiones del Estado y BoletÍn Ofi cial. 600p.
ROBIN, J.H.; REGOST, C.; ARZEL, J.; KAUSHIK,S.J. 2003 Fatty acid profile of fish following a change in dietary fatty acid source: model of fatty acid composition with a dilution hypothesis.Aquaculture, Amsterdam, 225: 283-293.
SARGENT, J.; BELL, G.; MCEVOY, L.; TOCHER, D.; ESTEVEZ, A. 1999 Recent developments in the essential fatty acid nutrition of fi sh. Aquaculture, Amsterdam, 177: 191-199.
SILFVERGRIP, A.M.C. 1996 A systematic revision of the Neotropical catfi sh genus Rhamdia (Teleostei, Pimelodidae). Stockholm: Department of Vertebrate Zoology/Swedish Museum of Natural History. 156p.
STEEL, R.G. y TORRIE, J.A. 1980 Principles and Procedures of statistics. A biometrical approach. New York: McGraw Hill. 633p.
TAKEUCHI, T. y WATANABLE, T. 1982 The effects of starvation and environmental temperature on proximate and fatty acid composition of carp and rainbow trout. Bulletin of the Japanese Society of Scientifi c Fisheries, 48: 1307-1316.
ULIANA, O.; SOUZA DA SILVA, J.H.; RADUNZ NETO, J. 2001 Substituição parcial ou total de Óleo de canola por lecitina de soja em rações para larvas de jundiá(Rhamdia quelen) PISCES, PIMELODIDAE. Ciencia Rural, 31: 677-681.
WEBER, P.C. 1990 The modifi cation of the arachidonic acid cascade by n-3 fatty acids. Advances in Prostaglandin, Thromboxane and Leukotriene Research, 20: 232-240.