Ankistrodesmus gracilis(Reisch) Korsikov (Chlorophyta) laboratory cultured in CHU12 and macrophyte with NPK media
Keywords:
algae, growth, biological aspects, nutritional contentAbstract
Two different culture media, namely CHU12 and inorganic fertilizer NPK (20-5-20) associated with macrophyte (Eichhornia crassipes) extract, were used to evaluate the development of A. gracilis. Growth rate, development, nutritional value and medium water quality were analyzed. A. gracilis in macrophyte+NPK medium had mean cell density (333 x 105 cells/mL) higher than medium CHU12 (302 x 105 cells/mL). Protein rate (12.52%PS), dry weight (397 x 107 pg/cell) and ashes (4.08 x 107 pg/cell) in A. gracilis was higher (p<0.05) than medium macrophyte+NPK. On the other hand, lipids, carbohydrates and fibers had the same rate (p>0.05) in both media. When hydrological variables in the culture medium of A. gracilis are taken into account, dissolved oxygen and free CO2 alone failed to have any significant difference (p>0.05) between the media employed. A. gracilis in the macrophyte+NPK medium had the same nutritional rate as the commercial medium CHU12, with excellent results for alga growth. Macrophyte medium may be used for large scale alga culture in the feed of fish larvae.
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STEIN, J. 1973 Handbook of phycological methods;Culture methods and growth measurements. Cambridge University Press. 447 p.
ALLGREN, G.; GUSTAFSSON, I.B.; BOBERG, M.1992 Fatty acid content and chemical
composition of freshwater microalgae.J.Phycol., 28: 37-50.
A.O.A.C. Association of Official Analytical Chemists. 1990 Official methods of analysis. 15TH ed., Arlington,Virginia, 683p.
BROWN, M.R.; JEFREY, S.W.; VOLKMAN, J.K.;DUSTAN, G.A. 1997 Nutritional propperties of micro-algae for marine culture.Aquaculture, 151: 315-331.
FAO í Organização das Nações Unidas para a Agricultura e Alimentação 2006 Aqüicultura mais do que uma indústria de exploração.Roma: FAO, 2006. Disponível em Internet (http://www.fao.org/spanish/newsroom/news/2003/21619-es.html. Acesso em: 18 abril 2006.
GOLTERMAN, H.L.; CLYMO, R. S.; OHNSTAD,M. A. 1978 Methods for physical and chemical analysis of freshwater. London: Blackwell Scientific Publication. 213p.
HARDY, E. R. e CASTRO, J. G. D. 2000 Qualidade nutricional de três espécies de clorofíceas cultivadas em laboratório. Acta Amazônica, 30:39/47.
ILLMAN, A.M.; SAAGG, A.H.; SHALES. S.W.2000 Increase in Chlorella strains calorific
values when grown in low nitrogen medium.Enzyme Microb. Tech., 27: 631-635.
KILHAM, S.S.; KREEGER, D.A.; GOULDEN, C.E.;LYNN, S.G. 1997 Effects of algal food quality on fecundity and population growth rates of Daphnia. Fresh. Biol., 38: 639-647.
KNUCKEY, R. M.; BROWN, M. R.; ROBERT, R.;FRAMPTON, D. M. F. 2006 Production of
micro-algal concentrates by flocculation and their assessment as aquaculture feeds.
Aquacultural Engineering, 35: 300-313.
KOROLEFF, F. 1976 Determination of nutrients.In: GRASSNOF, E. e KREMLING, E. (Ed.). ethods of seawater analysis. New York: Verlag Chemie, Wenhein. p.117-181.
MACKERETH, F. J. H.; HERON, J.; TALLING, J.F. 1978 Water analysis: some revised methods for limnologists. Oxford: Titus Wilson and Sons Ltda. Freshwater Biological Association.Scientific Publication, n. 36. 121p.
McKIM, S.M. e DURNFORD, D.G. 2006 Translational regulation of light-harvesting
complex expression during photo acclimation to high-light in Chlamydomonas reinhardtii.Plant Physiology and Biochemistry, 44: 857-865.
MESECK, S.L.; ALIX, J.H.; WIKFORS, G.H. 2005 Photoperiod and light intensity effects on growth and utilization of nutrients by aquaculture feed microalga, Tetraselmis chui (PLY 429). Aquaculture, 246: 393-404.
MORRIS, H. 1999 Composición bioquímica y evaluación de la calidade protéica de la
biomasa autotrófica de Chlorella vulgaris.Revista Cubana de Alimentación y Nutrición, 13:2-14.
NUSH, E. A. 1980 Comparison of different methods for chlorophyll and phaeopigments determination. Archiv für Hydrobiol, 14: 14-36.
PÉREZ, E.B.; PINA, J.C.; RODRIGUEZ, L.P. 2008 Kinetic model for growth of Phaeodactylum tricornutum in intensive culture photobioreactor. Biochemical Engineering Journal, 40: 520-525.
RHEE, G.Y. 1978 Effects of N:P atomic rations and nitrate limitation on algal growth, cell composition, and nitrate uptake. Limnol.Oceanogr., 23: 10-25.
SIPAíÅ¡BA-TAVARES, L.H. 1995 Limnologia Aplicada í Aqüicultura. Boletim Técnico
número1, FUNEP, Jaboticabal, SP. 70p.
SIPAíÅ¡BA-TAVARES, L.H. e ROCHA, 1993 Cultivo em larga escala de organismos
planctônicos para alimentação de larvas e alevinos de peixes: I- algas clorofíceas.
Biotemas, 6: 93-106.
SIPAíÅ¡BA-TAVARES, L.H.; PELICIONI, L. C.;OLIVERA, A. 1999 Use of inorganic (NPK)
and the CHU12 medium for cultivation of Ankistrodesmus gracilis in laboratory. Brazilian Journal of Ecology, 1: 10-15.
SIPAíÅ¡BA-TAVARES, L.H. e BACHION, M. A. 2002 Population growth and development of two species of Cladocera, Moina micrura and Diaphanosoma birgei, in laboratory. Braz. J.Biol., 62: 701-711.
SIPAíÅ¡BA-TAVARES, L.H.; FAVERO, E.G.P.;BRAGA, F.M. de S. 2002 Utilization of
macrophyte biofilter in effluent from aquaculture: I- Floating Plant. Braz. J. Biol., 62:
713-723.
SIPAíÅ¡BA-TAVARES, L.H. e BRAGA, F.M. de S.2007 Feeding activity of Colossoma
macropomum larvae (tambaqui) in fishpond with water hyacinth (Eichhornia crassipes)fertilizer. Braz. J. Biol., 67: 459-466.
SIPAíÅ¡BA-TAVARES, L.H. e PEREIRA, A.M.L.2008 Large scale laboratory cultures of
Ankistrodesmus gracilis (Reisch) Korsikov (Chlorophyta) and Diaphanosoma birgei
Korinek, 1981 (Cladocera). Braz. J. Biol., 68:875-883.
SMIT, A.J.; ROBERTSON, B.L.; DU-PEREZ, D.R.1997 Influence of ammonium í N pulse
concentrations and frequency, tank condition ad nitrogen starvation on growth rate and biochemical copmposition of Gracilaria gracilis. J.Appl. Phycol., 8: 473-481.
SOUZA, J.S.I. de 2004 Enciclopédia Agrícola Brasileira. SãoPaulo: Editora da Universidade de São Paulo-EDUSP/ESALQ. 512p.
STEIN, J. 1973 Handbook of phycological methods;Culture methods and growth measurements. Cambridge University Press. 447 p.
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2018-11-04
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