Morphological and molecular analysis of Eustrongylides sp. (Nematoda: Dioctophymatidae) in zebrafish (Danio rerio) from Brazil

Authors

  • Melissa Querido Cárdenas Fundação Oswaldo Cruz – Instituto Oswaldo Cruz – Laboratório de Helmintos Parasitos de Peixes – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0003-4384-357X
  • Gabriel de Farias Araujo Fundação Oswaldo Cruz – Instituto Oswaldo Cruz – Laboratório de Avaliação e Promoção da Saúde Ambiental – Rio de Janeiro (RJ), Brazil | Fundação Oswaldo Cruz – Escola Nacional de Saúde Pública – Programa de Pós-Graduação em Saúde Pública e Meio Ambiente – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0002-6511-0388
  • Jucicleide Ramos-de-Souza Fundação Oswaldo Cruz – Instituto Oswaldo Cruz – Laboratório de Malacologia – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0002-8313-2310
  • Amanda Gleyce Lima de Oliveira Fundação Oswaldo Cruz – Instituto Oswaldo Cruz – Laboratório de Avaliação e Promoção da Saúde Ambiental – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0002-8599-554X
  • Enrico Mendes Saggioro Fundação Oswaldo Cruz – Instituto Oswaldo Cruz – Laboratório de Avaliação e Promoção da Saúde Ambiental – Rio de Janeiro (RJ), Brazil. https://orcid.org/0000-0002-6072-6749

DOI:

https://doi.org/10.20950/1678-2305/bip.2024.51.e936

Keywords:

Fish nematode, 18S rRNA, Parasitism, Health monitoring

Abstract

The zebrafish, Danio rerio, is an important vertebrate model organism across various scientific disciplines. The current study reports the nematode larva, Eustrongylides sp., presence, in zebrafish from a pet shop, acquired by Fundação Oswaldo Cruz for toxicity studies. Morphological and molecular data from this nematode are given herein, contributing to the understanding of this helminth and aiding future studies. The occurrence of this larva demonstrates the importance of controlling the management of D. rerio, since this fish is widely used as an experimental model.

References

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2

American Veterinary Medical Association (AVMA). (2013). AVMA guidelines for the euthanasia of animals. AVMA. Amsterdam, A., & Hopkins, N. (2006). Mutagenesis strategies in zebrafish for identifying genes involved in development and disease. Trends in Genetics, 22(9), 473-478. https://doi.org/10.1016/j.tig.2006.06.011

Associação Brasileira de Normas Técnicas (ABNT) (2016). NBR 15088/16. Ecotoxicologia aquática - Toxicidade aguda - Método de ensaio com peixes (Cyprinidae) (3ª ed.). ABNT.

Banzai, A., Tanikawa, T., Kimura, G., Sasaki, T., & Kawakami, Y. (2018). Parasitic helminths collected from the brown rat, Rattus norvegicus, in Chuo Ward, Tokyo, Japan. Medical Entomology and Zoology, 69(4), 171-176. https://doi.org/10.7601/mez.69.171

Barros, L. A., Oliveira, R. D., Moraes Filho, J., Justino, C. D. S., & Mateus, L. D. F. (2009). Análise do parasitismo por Contracaecum sp. e Eustrongylides sp. em cacharas, Pseudoplatystoma fasciatum (Linnaeus, 1766) (Pisces: Pimelodidae) provenientes do rio Cuiabá, Mato Grosso, Brasil. Revista Brasileira de Ciência Veterinária, 16(2), 58-61. https://doi.org/10.4322/rbcv.2014.170

Blaxter, M. L., De Ley, P., Garey, J., Liu, L. X., Scheldeman, P., Vierstraete, A., Vanfleteren, J. R., Mackey, L. Y., Dorris, M., Frisse, L. M., Vida, J. T., & Thomas, W. K. (1998). A molecular evolutionary framework for the phylum Nematoda. Nature, 392, 71-75. https://doi.org/10.1038/32160

Borski, R. J., & Hodson, R. G. (2003). Fish Research and the Institutional Animal Care and Use Committee. ILAR Journal, 44(4), 286-294. https://doi.org/10.1093/ilar.44.4.286

Braunbeck, T., & Lammer, E. (2006). Fish embryo toxicity assays. Contract, 20, 725-731. Canadian Council on Animal Care (CCAC) (2005). Guidelines on the care and use of fish in research, teaching and testing. CCAC. Conselho Nacional de Controle de Experimentação Animal (CONCEA) (2015). Diretriz da prática de eutanásia do CONCEA. Anexo I - Diretriz referente a procedimentos de eutanásia realizados em animais incluídos em atividades de ensino ou de pesquisa científica, Brasil. CONCEA.

Correa, L. L., Soares, G. B., Müller, M. I., & Adriano, E. A. (2023). First morphological and molecular characterization of the genus Eustrongylides nematode larvae infecting fish in the Brazilian Amazon. Biologia, 78(10), 2855-2862. https://doi.org/10.1007/s11756-023-01422-6

Eiras, J. C., Pavanelli, G. C., Takemoto, R. M., Yamaguchi, M. U., Karkling, L. C., & Nawa, Y. (2016). Potential risk of fish-borne nematode infections in humans in Brazil–current status based on a literature review. Food and Waterborne Parasitology, 5, 1-6. https://doi.org/10.1016/j.fawpar.2016.08.002

Floyd, R., Abebe, E., Papert, A., & Blaxter, M. (2002). Molecular barcodes for soil nematode identification. Molecular Ecology, 11(4), 839-850. https://doi.org/10.1046/j.1365-294X.2002.01485.x

Franceschini, R., Guardone, L., Armani, A., Ranucci, D., Roila, R., Valiani, A., Susini, F., & Branciari, R. (2022). Five-years management of an emerging parasite risk (Eustrongylides sp., Nematoda) in a fishery supply chain located on Trasimeno Lake (Italy). Food Control, 136, 108858. https://doi.org/10.1016/j.foodcont.2022.108858

Fusco, M. A., Rizzo-Valente, V. D. S., Vizzoni, V. F., Miranda, R. S. D., Aguiar, C. C. D., & Escaleira, R. D. C. (2023). An outbreak of the nematode parasite Eustrongylides spp. (Nematoda: Dioctophymatidae) in a zebrafish (Danio rerio) facility. Zebrafish, 20(3), 126-130. https://doi.org/10.1089/zeb.2022.0041

Grunwald, D. J., & Eisen, J. S. (2002). Headwaters of the zebrafish: emergence of a new model vertebrate. Nature Reviews Genetics, 3(9), 717-724. https://doi.org/10.1038/nrg892

Gueretz, J. S., Senger, S. B., & Claus, M. P. (2020). Ocorrência de Ithyoclinostomum sp. e Eustrongylides sp., parasitos de Hoplias aff. malabaricus Bloch, 1794 (Characiformes: Erythrinidae), no litoral de Santa Catarina, Brasil. Brazilian Journal of Development, 6(4), 21565-21575. https://doi.org/10.34117/bjdv6n4-359

Haque, E., & Ward, A. C. (2018). Zebrafish as a model to evaluate nanoparticle toxicity. Nanomaterials, 8(7), 561. https://doi.org/10.3390/nano8070561

Hodda, M. (2022a). Phylum Nematoda: a classification, catalogue and index of valid genera, with a census of valid species. Zootaxa, 5114(1), 1-289. https://doi.org/10.11646/zootaxa.5114.1.1

Hodda, M. (2022b). Phylum Nematoda: trends in species descriptions, the documentation of diversity, systematics, and the species concept. Zootaxa, 5114(1), 290-317. https://doi.org/10.11646/zootaxa.5114.1.2

Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., & Helder, J. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown Clades. Molecular Biology and Evolution, 23(9), 1792 1800. https://doi.org/10.1093/molbev/msl044

Honcharov, S. L., Soroka, N. M., Galat, M. V., Zhurenko, O. V., Dubovyi, A. I., & Dzhmil, V. I. (2022). Eustrongylides (Nematoda: Dioctophymatidae): Epizootology and special characteristics of the development biology. Helminthologia, 59(2), 127-142. https://doi.org/10.2478/helm-2022-0013

Howe, K., Clark, M., Torroja, C., Torrance, J., Berthelot, C., Muffato, M., Collins, J. E., Humphray, S., McLaren, K., Matthews, L., McLaren, S., Sealy, I., Caccamo, M., Churcher, C., Scott, C., Barrett, J. C., … & Stemple, D. L. (2013). The zebrafish reference genome sequence and its relationship to the human genome. Nature, 496, 498-503. https://doi.org/10.1038/nature12111

Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B., & Schilling, T. F. (1995). Stages of embryonic development of the zebrafish. Developmental Dynamics, 203(3), 253-310. https://doi.org/10.1002/aja.1002030302

Kumar, V., Roy, S., Parida, S. N., Bisai, K., Dhar, S., Jana, A. K., & Das, B. K. (2024). Deciphering the impact of endoparasitic infection on immune response and gut microbial composition of Channa punctata. Frontiers in Cellular and Infection Microbiology, 14, 1296769. https://doi.org/10.3389/fcimb.2024.1296769

Kuraiem, B. P., Knoff, M., Telleria, E. L., Fonseca, M. C. G., Machado, L. S., Cunha, N. C., Nascimento, E. R., Fontenelle, G., Gomes, D. C., & São Clemente, S. C. (2020). Eustrongylides sp. (Nematoda, Dioctophymatoidea) parasitizing Hoplias malabaricus (Actinopterygii: Erythrinidae) collected from the state of Rio de Janeiro, Brazil. Revista Brasileira de Parasitologia Veterinária, 29(1), e014519. https://doi.org/10.1590/S1984-29612019094

Lignon, J. S., Stainki, T. M., & Monteiro, S. G. (2023). Eustrongylides spp. (Nematoda: Eustrongylidae) in Polypterus endlicheri in central Rio Grande do Sul, Brazil. Ciência Animal Brasileira, 24, e-74213. https://doi.org/10.1590/1809-6891v24e-74213E

Lima, F., Pozza, A., & Lehmann, P. (2019). Contracaecum spp. (Nematoda: Anisakidae) and Eustrongylides spp. (Nematoda: Dioctophymatidae) nematode larvae with zoonotic potencial found in two fish species from Tramandaí river basin, southern Brazil. Boletim do Instituto de Pesca, 45(3), e495. https://doi.org/10.20950/1678-2305.2019.45.3.495

Martins, M. L., de Souza Santos, R., Marengoni, N. G., Takahashi, H. K., & Onaka, E. M. (2009). Sazonalidade de larvas de Eustrongylides sp. (Nematoda: Dioctophymatidae) em peixes do Rio Paraná, Sudeste do Brasil. Boletim do Instituto de Pesca, 35(1), 29-37.

Mazzone, A., Cafara, M., Gustinelli, A., Agnetti, F., Sgariglia, E., Vaglio, G. L., Quaglio, F., & Fioravanti, M. L (2019). Morphological and molecular characterization of larval and adult stages of Eustrongylides excisus (Nematoda: Dioctophymatoidea) with histopathological observations. Journal of Parasitology, 105(6), 882 889. https://doi.org/10.1645/19-44

Moravec, F. (1998). Nematodes of freshwater fishes of the Neotropical Region. Academia. Organisation for Economic Co-Operation and Development (OECD) (1992). OECD 203/92 Guideline for testing of chemicals. Fish, acute toxicity test. OECD.

Padial, J. M., Miralles, A., De la Riva, I., & Vences, M. (2010). The integrative future of taxonomy. Frontiers in Zoology, 7, 16. https://doi.org/10.1186/1742-9994-7-16

Pekmezci, G. Z., & Bolukbas, C. S. (2021). Morphological and molecular characterization of Eustrongylides excisus larvae (Nematoda: Dioctophymatidae) in Sander lucioperca (L.) from Northern Turkey. Parasitology Research, 120(6), 2269-2274. https://doi.org/10.1007/s00436-021-07187-8

Rubinstein, A. L. (2003). Zebrafish: from disease modelling to drug discovery. Current Opinion in Drug Discovery Development, 6(2), 218-223.

Shamsi, S., Francis, N., Masiga, J., Barton, D. P., Zhu, X., Pearce, L., & McLellan, M. (2023). Occurrence and characterisation of Eustrongylides species in Australian native birds and fish. Food and Waterborne Parasitology, 30, e00189. https://doi.org/10.1016/j.fawpar.2023.e00189

Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(7), 3022-3027. https://doi.org/10.1093/molbev/msab120

Vascotto, S. G., Beckham, Y., & Kelly, G. M. (1997). The zebrafish’s swim to fame as an experimental model in biology. Biochemistry and Cell Biology, 75(5), 479-485. https://doi.org/10.1139/o97-081

WoRMS Editorial Board (2024). World Register of Marine Species. WoRMS Editorial Board. https://doi.org/10.14284/170

Xiong, F., Li, W. X., Wu, S. G., Zou, H., & Wang, G. T. (2013). Molecular phylogeny and host specificity of the larval Eustrongylides (Nematoda: Dioctophmidae) from freshwater fish in China. Journal of Parasitology, 99(1), 137-144. https://doi.org/10.1645/GE-3163.1

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Published

2025-01-28

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