EFICIíÅ NCIA DE ANESTÉSICOS EM FíÅ MEAS DO GASTRÓPODE Littoraria angulifera
DOI:
https://doi.org/10.20950/1678-2305.2018.304Palavras-chave:
eugenol, cloreto de magnésio, mentol, moluscos, sedaçãoResumo
O presente estudo avaliou o efeito de três anestésicos em três concentrações, o mentol (C10H20O) (1%; 1,5%; 2%), o eugenol (C10H12O2) (0,5%; 1%; 1,5%) e o cloreto de magnésio (MgCl2) (4%;7%;10%), diluídos em água destilada, sobre fêmeas do gastrópode Littoraria angulifera. Para cada concentração foram utilizadas 20 fêmeas (total = 180), com altura da concha entre 18,3 e 26,1 mm, coletadas no mesmo local. Foram observados os tempos de indução e de recuperação do efeito sedativo, assim como a sobrevivência. Os animais foram mantidos em aquários a uma temperatura laboratorial de 25±1°C e as observações foram feitas a cada 30 minutos após a anestesia, durante 2 horas. Após o término do experimento, os animais foram mantidos nos aquários e observados por 15 dias quanto í sobrevivência. Apenas o MgCl2 causou efeito sedativo sobre L. angulifera, sendo que a 4% e a 7% observou-se sedação de 20% e de 95% dos animais, respectivamente, após 2 horas de anestesia, enquanto que a 10%, todos os animais foram anestesiados em 1 hora. Em conclusão, L. angulifera pode ser anestesiada de forma eficiente em uma solução de 10% de MgCl2 durante 1 h e a sua recuperação ocorre ao fim de 30 minutos sem nenhum efeito colateral evidente no comportamento e na sobrevivência.
Referências
ACOSTA-SALMON, H.; DAVIS, M. 2007 Inducing relaxation in the queen conch Strombus gigas (L.) for cultured pearl production. Aquaculture, 262(1): 73-77.
ACOSTA-SALMON, H.; MARTINEZ-FERNANDEZ, E.; SOUTHGATE, P.C. 2005 Use of relaxants to obtain saibo tissue from the blacklip pearl oyster (Pinctada margaritifera) and the Akoya pearl oyster (Pinctada fucata). Aquaculture, 246(1-4): 167-172.
ALIPIA, T.T.; MAE, H.; DUNPHY, B.J. 2014 A non-invasive anaesthetic method for accessing the brood chamber of the Chilean flat oyster (Ostrea chilensis). New Zealand Journal of Marine and Freshwater Research, 48(3): 350-355.
ARAUJO, R.; REMÓN, J.M.; MORENO, D.; RAMOS, M.A. 1995 Relaxing techniques for freshwater molluscs: trials for evaluation of different methods. Malacologia, 36(1): 29-41.
BARROSO, C.M.; GIBBS; MOREIRA, M.H.; GIBBS, P.E. 2000 Comparison of imposex and intersex development in four prosobranch species for TBT monitoring of a southern European estuarine system (Ria de Aveiro, NW Portugal). Marine Ecology Progress Series, 201(3): 221-232.
BERRY, A. J. 1963 Faunal zonation in mangrove swamps. Bulletin of the National Museum, State of Singapore, 32(1): 90-98.
BILBAO, A.; COURTOIS DE VICOSE G.; VIERA, M.P.; SOSA, B.; FERNÁNDEZ-PALACIOS, H.; HERNANDÉZ, M.C 2010 Efficiency of Clove Oil as Anesthetic for Abalone (Haliotis Tuberculata Coccinea, Revee). Journal of Shellfish Research, 29(3): 679-682.
BRYAN, G.W.; GIBBS, P.E. 1991 Impact of low concentration of tributyltin (TBT) on marine organisms: a review. In: NEWMAN, M.C., MCINTOSH, AW. Metal Ecotoxicology: Concepts and applications. Ann Arbor: Lewis Publishers. p. 323-361.
BUTT, D.; O'CONNOR, S.J.; KUCHEL, R.; O'CONNOR, W.A.; RAFTOS, D.A. 2008 Effects of the muscle relaxant, magnesium chloride, on the Sydney rock oyster (Saccostrea glomerata). Aquaculture, 275(1-4): 342-346.
CASTRO, I.B.; LIMA, A.F.; BRAGA, A.R.C.; ROCHA-BARREIRA, C.A. 2007 Imposex in two muricid species (Mollusca: Gastropoda) from the northeastern Brazilian coast. Journal of Brazilian Society of Ecotoxicology, 2(1): 81-91.
CULLOTY, S.C.; MULCAHY, M.F. 1992. An evaluation for anesthetics for Ostrea edulis. Aquaculture, 107(2-3):249-252.
EMBERTON, K.C. 1989 Retraction/extension and measurement error in a land snail: effects on systematic characters. Malacologia, 31(1):157-173.
FAí"¡ANHA, M.F.; GOMES, L.C. 2005 A eficácia do mentol como anestésico para tambaqui (Colossoma macropomum, Characiformes: Characidae). Acta Amazônica, 35(1): 71-75.
GIRDLESTONE, D.; CRUICKSHANK, S.G.H.; WINLOW, W. 1989 The actions of three volatile general anaesthetics on withdrawal responses on the pond snail Lymnaea stagnalis L. Comparative Biochemistry and Physiology. C-Comparative Pharmacology and Toxicology, 92(1): 39-44.
GOMES, L.C. 2001 Efficacy of benzocaine as an anesthesic in juvenile tambaqui Colossoma macropomum. Journal of World Aquactic Society, Baton Rouge, 32(4): 426-431.
HEASMAN, M.P.; O’CONNOR, W.A.; FRAZER, A.W.J. 1995 Induction of anaesthesia in the commercial scallop, Pecten fumatus Reeve. Aquaculture, 131(3-4): 231-238.
IVERSEN, M.; FINSTAD, B.; McKINLEY, R.S.; ELIASSEN, R.A. 2003 The efficacy of metomidate, clove oil, AQUI-Sâ"ž¢ and Benzoak® as anaesthetics in Atlantic salmon (Salmo salar L.) smolts, and their potencial stress-reducing capacity. Aquaculture, 221(1-4): 549-566.
KANDEL, E.R.; SCHWARTZ, J.H.; JESSELL, T.M. 2000 Principles of neural science. 4th ed. New York: McGraw-Hill. 1414p.
KAPLAN, H.M. 1969 Anesthesia in amphibians and reptiles. 28 ed. Federal Proceeding. 1546p.
KEENE, J.L.; NOAKES, D.L.G.; MOCCIA, R.D.; SOTO, C.G. 1998 The efficacy of clove oil as an anaesthesic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 29(2):89-101.
KOHLMEYER, J.; BEBOUT, B. 1986 On the occurrence of marine fungi in the diet of Littorina angulifera and observations on the behavior of the periwinkle. Marine Ecology, 7(4): 333-343.
LEGAT, A.P.; J.; LEGAT, J.F.A.; GOMES, C.H.A.M.; SUHNEL, S.; MELO, C.M.R. 2015 Anesthesia in oysters of the genus Crassostrea cultured in Brazil. Boletim do Instituto da Pesca, 41(esp): 785í 793.
LENDERKING, R.E. 1954 Some recent observations on the biology of Littorina angulifera of Biscayne and Virginia Keys, Florida. Bulletin of Marine Science of the Gull and Caribbean, 3(4): 272-296.
LORENZO, D.; PAZ, D., DELLACASSA, E. 2002 Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay. Brazilian Archives of Biology and Technology, 45(4): 519-524.
MAMANGKEY, N.G.F.; ACOSTA-SALMON, H.; SOUTHGATE, P.C 2009 Use of anaesthetics with the silver-lip pearl oyster, Pinctada maxima (Jameson). Aquaculture, 288(3-4): 280-284.
MCSHANE, P.E.; SMITH, M.G 1988. Measuring recruitment of abalone Haliotis rubra Leach (Gastropoda:Haliotidae) - comparison of a novel method with two other methods. Australian Journal of Marine Freshwater Research. 39(3): 331-336.
MEIER-BROOK, C. 1976 The influence of varied relaxing and fixing conditions on anatomical characters in a Planorbis species. Basteria, 40(4-6): 101-106.
MERKT, R.E.; ELLISON, A.M. 1998 Geographic and habitat-specific morphological variation of Littoraria (Littorinopsis) angulifera (Lamarck, 1822). Malacologia, 40(1): 279-295.
PRINCE J.D.; FORD W.B. 1985 Use of anaesthetic to standardize efficiency in sampling abalone populations (genus Haliotis; Mollusca : Gastropoda). Australian Journal of Marine Freshwater Research, 36(2): 701-706.
OSTRENSKY, A.; BORGHETTI, J.R.; SOTO, D. 2008 Aquicultura no Brasil: o desafio é crescer. Secretaria Especial da Aquicultura e Pesca. Brasília, 2008.
REID, D.G. 1999 The genus Littoraria Griffith & Pidgeon, 1834 (Gastropoda: Littorinidae) in the tropical eastern Pacific. Veliger, 42(1): 21-53.
REID, D. G.; DYAL, P.; WILLIAMS, S. T. 2010 Global diversification of mangrove fauna: a molecular phylogeny of Littoraria (Gastropoda: Littorinidae). Molecular Phylogenetics and Evolution, 55(1): 185-201.
ROSS, L.G.; ROSS, B. 2008 Anaesthetic and sedative techniques for aquatic animals. 3rd. ed. Oxford: Blackwell Science, 236p.
RUNHAM, N.W.; ISARANKURA, K.; SMITH, B.J. 1965 Methods for narcotizing and anaesthetizing gastropods. Malacologia, 2(2): 231-238.
SANTOS, D.M.; SANT’ANNA, B.S.; GODOI, A.F.L.; TURRA, A.; MARCHI, M.R.R. 2011 Contamination and Impact of Organotin Compounds on the Brazilian Coast. In: Ortiz, AC., Grifï¬Ân NB. Pollution Monitoring. Hauppauge NY: N. Science Publishers. p. 31-59.
SOUSA, A.; LARANJEIRO, F.; TAKAHASHI, S.; TANABE, S.; BARROSO, C.M. 2009 Imposex and organotin prevalence in a European post-legislative scenario: temporal trends from 2003 to 2008. Chemosphere, 77(4): 566-573.
SOUSA, A.; MENDO, S.; BARROSO, C.M. 2005 Imposex and organotin contamination in Nassarius reticulatus (L.) along the Portuguese coast. Applied Organometallic Chemistry, 19(3): 315-323.
SOUSA, A.C.A.; BARROSO, C.M.; TANABE, S.; HORIGUCHI, T. 2010 Involvement of Retinoid X Receptor in Imposex Development in Nucella lapillus and Nassarius reticulatus-Preliminary Results. Interdisciplinary Studies on Environmental Chemistry, 4(1): 189-196.
SUQUET, M.; KERMOYSAN, G.; ARAYA, R.G.; QUEAU, I.; LEBRUN, L.; SOUCHU, P.; MINGANT, C. 2009 Anesthesia in Pacific oyster, Crassostrea gigas. Aquatic Living Resource, 22(1): 29-34.
VASCONCELOS, P.; GASPAR, M.B.; PEREIRA, A.M.; CASTRO, M. 2006. Growth rate estimation of Hexaplex (trunculariopsis) trunculus (Gastropoda: Muricidae) based on mark/recapture experiments in the Ria Formosa lagoon (Algarve coast, southern Protugal). Journal of Shellfish Research, 25(1): 249-256.
WAGNER, E.; ARNDT, R.; HILTON, B. 2002 Physiological stress responses, egg survival and sperm motility of rainbow trout broodstock anesthezid with clove oil, tricaine methanesulfonate or carbon dioxide. Aquaculture, 211(1-4): 353-366.
WHITE, H.I.; HECHT, T.; POTGEITER, B. 1996 The effect of four anesthetics on Haliotis midae and their suitability for application in commercial abalone culture. Aquaculture, 140(1-2): 145-151.
WOODALL, A.J.; NARUO, H.; PRINCE, D.J.; FENG, Z.P.; WINLOW, W.; TAKASAKI, M.; SYED, N.I. 2003 Anesthetic treatment blocks synaptogenesis but not neuronal regeneration of cultured Lymnaea neurons. Journal of Neurophysiology, 90(4): 2232-2239.
ZEIDAN, G.C. 2017 Efeito do tributil-estanho (TBT) sobre Stramonita rustica (Neogastropoda) e Littoraria angulifera (Mesogastropoda). Ilhéus (BA), 189p. (Tese de Doutorado. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz).
ACOSTA-SALMON, H.; MARTINEZ-FERNANDEZ, E.; SOUTHGATE, P.C. 2005 Use of relaxants to obtain saibo tissue from the blacklip pearl oyster (Pinctada margaritifera) and the Akoya pearl oyster (Pinctada fucata). Aquaculture, 246(1-4): 167-172.
ALIPIA, T.T.; MAE, H.; DUNPHY, B.J. 2014 A non-invasive anaesthetic method for accessing the brood chamber of the Chilean flat oyster (Ostrea chilensis). New Zealand Journal of Marine and Freshwater Research, 48(3): 350-355.
ARAUJO, R.; REMÓN, J.M.; MORENO, D.; RAMOS, M.A. 1995 Relaxing techniques for freshwater molluscs: trials for evaluation of different methods. Malacologia, 36(1): 29-41.
BARROSO, C.M.; GIBBS; MOREIRA, M.H.; GIBBS, P.E. 2000 Comparison of imposex and intersex development in four prosobranch species for TBT monitoring of a southern European estuarine system (Ria de Aveiro, NW Portugal). Marine Ecology Progress Series, 201(3): 221-232.
BERRY, A. J. 1963 Faunal zonation in mangrove swamps. Bulletin of the National Museum, State of Singapore, 32(1): 90-98.
BILBAO, A.; COURTOIS DE VICOSE G.; VIERA, M.P.; SOSA, B.; FERNÁNDEZ-PALACIOS, H.; HERNANDÉZ, M.C 2010 Efficiency of Clove Oil as Anesthetic for Abalone (Haliotis Tuberculata Coccinea, Revee). Journal of Shellfish Research, 29(3): 679-682.
BRYAN, G.W.; GIBBS, P.E. 1991 Impact of low concentration of tributyltin (TBT) on marine organisms: a review. In: NEWMAN, M.C., MCINTOSH, AW. Metal Ecotoxicology: Concepts and applications. Ann Arbor: Lewis Publishers. p. 323-361.
BUTT, D.; O'CONNOR, S.J.; KUCHEL, R.; O'CONNOR, W.A.; RAFTOS, D.A. 2008 Effects of the muscle relaxant, magnesium chloride, on the Sydney rock oyster (Saccostrea glomerata). Aquaculture, 275(1-4): 342-346.
CASTRO, I.B.; LIMA, A.F.; BRAGA, A.R.C.; ROCHA-BARREIRA, C.A. 2007 Imposex in two muricid species (Mollusca: Gastropoda) from the northeastern Brazilian coast. Journal of Brazilian Society of Ecotoxicology, 2(1): 81-91.
CULLOTY, S.C.; MULCAHY, M.F. 1992. An evaluation for anesthetics for Ostrea edulis. Aquaculture, 107(2-3):249-252.
EMBERTON, K.C. 1989 Retraction/extension and measurement error in a land snail: effects on systematic characters. Malacologia, 31(1):157-173.
FAí"¡ANHA, M.F.; GOMES, L.C. 2005 A eficácia do mentol como anestésico para tambaqui (Colossoma macropomum, Characiformes: Characidae). Acta Amazônica, 35(1): 71-75.
GIRDLESTONE, D.; CRUICKSHANK, S.G.H.; WINLOW, W. 1989 The actions of three volatile general anaesthetics on withdrawal responses on the pond snail Lymnaea stagnalis L. Comparative Biochemistry and Physiology. C-Comparative Pharmacology and Toxicology, 92(1): 39-44.
GOMES, L.C. 2001 Efficacy of benzocaine as an anesthesic in juvenile tambaqui Colossoma macropomum. Journal of World Aquactic Society, Baton Rouge, 32(4): 426-431.
HEASMAN, M.P.; O’CONNOR, W.A.; FRAZER, A.W.J. 1995 Induction of anaesthesia in the commercial scallop, Pecten fumatus Reeve. Aquaculture, 131(3-4): 231-238.
IVERSEN, M.; FINSTAD, B.; McKINLEY, R.S.; ELIASSEN, R.A. 2003 The efficacy of metomidate, clove oil, AQUI-Sâ"ž¢ and Benzoak® as anaesthetics in Atlantic salmon (Salmo salar L.) smolts, and their potencial stress-reducing capacity. Aquaculture, 221(1-4): 549-566.
KANDEL, E.R.; SCHWARTZ, J.H.; JESSELL, T.M. 2000 Principles of neural science. 4th ed. New York: McGraw-Hill. 1414p.
KAPLAN, H.M. 1969 Anesthesia in amphibians and reptiles. 28 ed. Federal Proceeding. 1546p.
KEENE, J.L.; NOAKES, D.L.G.; MOCCIA, R.D.; SOTO, C.G. 1998 The efficacy of clove oil as an anaesthesic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 29(2):89-101.
KOHLMEYER, J.; BEBOUT, B. 1986 On the occurrence of marine fungi in the diet of Littorina angulifera and observations on the behavior of the periwinkle. Marine Ecology, 7(4): 333-343.
LEGAT, A.P.; J.; LEGAT, J.F.A.; GOMES, C.H.A.M.; SUHNEL, S.; MELO, C.M.R. 2015 Anesthesia in oysters of the genus Crassostrea cultured in Brazil. Boletim do Instituto da Pesca, 41(esp): 785í 793.
LENDERKING, R.E. 1954 Some recent observations on the biology of Littorina angulifera of Biscayne and Virginia Keys, Florida. Bulletin of Marine Science of the Gull and Caribbean, 3(4): 272-296.
LORENZO, D.; PAZ, D., DELLACASSA, E. 2002 Essential oils of Mentha pulegium and Mentha rotundifolia from Uruguay. Brazilian Archives of Biology and Technology, 45(4): 519-524.
MAMANGKEY, N.G.F.; ACOSTA-SALMON, H.; SOUTHGATE, P.C 2009 Use of anaesthetics with the silver-lip pearl oyster, Pinctada maxima (Jameson). Aquaculture, 288(3-4): 280-284.
MCSHANE, P.E.; SMITH, M.G 1988. Measuring recruitment of abalone Haliotis rubra Leach (Gastropoda:Haliotidae) - comparison of a novel method with two other methods. Australian Journal of Marine Freshwater Research. 39(3): 331-336.
MEIER-BROOK, C. 1976 The influence of varied relaxing and fixing conditions on anatomical characters in a Planorbis species. Basteria, 40(4-6): 101-106.
MERKT, R.E.; ELLISON, A.M. 1998 Geographic and habitat-specific morphological variation of Littoraria (Littorinopsis) angulifera (Lamarck, 1822). Malacologia, 40(1): 279-295.
PRINCE J.D.; FORD W.B. 1985 Use of anaesthetic to standardize efficiency in sampling abalone populations (genus Haliotis; Mollusca : Gastropoda). Australian Journal of Marine Freshwater Research, 36(2): 701-706.
OSTRENSKY, A.; BORGHETTI, J.R.; SOTO, D. 2008 Aquicultura no Brasil: o desafio é crescer. Secretaria Especial da Aquicultura e Pesca. Brasília, 2008.
REID, D.G. 1999 The genus Littoraria Griffith & Pidgeon, 1834 (Gastropoda: Littorinidae) in the tropical eastern Pacific. Veliger, 42(1): 21-53.
REID, D. G.; DYAL, P.; WILLIAMS, S. T. 2010 Global diversification of mangrove fauna: a molecular phylogeny of Littoraria (Gastropoda: Littorinidae). Molecular Phylogenetics and Evolution, 55(1): 185-201.
ROSS, L.G.; ROSS, B. 2008 Anaesthetic and sedative techniques for aquatic animals. 3rd. ed. Oxford: Blackwell Science, 236p.
RUNHAM, N.W.; ISARANKURA, K.; SMITH, B.J. 1965 Methods for narcotizing and anaesthetizing gastropods. Malacologia, 2(2): 231-238.
SANTOS, D.M.; SANT’ANNA, B.S.; GODOI, A.F.L.; TURRA, A.; MARCHI, M.R.R. 2011 Contamination and Impact of Organotin Compounds on the Brazilian Coast. In: Ortiz, AC., Grifï¬Ân NB. Pollution Monitoring. Hauppauge NY: N. Science Publishers. p. 31-59.
SOUSA, A.; LARANJEIRO, F.; TAKAHASHI, S.; TANABE, S.; BARROSO, C.M. 2009 Imposex and organotin prevalence in a European post-legislative scenario: temporal trends from 2003 to 2008. Chemosphere, 77(4): 566-573.
SOUSA, A.; MENDO, S.; BARROSO, C.M. 2005 Imposex and organotin contamination in Nassarius reticulatus (L.) along the Portuguese coast. Applied Organometallic Chemistry, 19(3): 315-323.
SOUSA, A.C.A.; BARROSO, C.M.; TANABE, S.; HORIGUCHI, T. 2010 Involvement of Retinoid X Receptor in Imposex Development in Nucella lapillus and Nassarius reticulatus-Preliminary Results. Interdisciplinary Studies on Environmental Chemistry, 4(1): 189-196.
SUQUET, M.; KERMOYSAN, G.; ARAYA, R.G.; QUEAU, I.; LEBRUN, L.; SOUCHU, P.; MINGANT, C. 2009 Anesthesia in Pacific oyster, Crassostrea gigas. Aquatic Living Resource, 22(1): 29-34.
VASCONCELOS, P.; GASPAR, M.B.; PEREIRA, A.M.; CASTRO, M. 2006. Growth rate estimation of Hexaplex (trunculariopsis) trunculus (Gastropoda: Muricidae) based on mark/recapture experiments in the Ria Formosa lagoon (Algarve coast, southern Protugal). Journal of Shellfish Research, 25(1): 249-256.
WAGNER, E.; ARNDT, R.; HILTON, B. 2002 Physiological stress responses, egg survival and sperm motility of rainbow trout broodstock anesthezid with clove oil, tricaine methanesulfonate or carbon dioxide. Aquaculture, 211(1-4): 353-366.
WHITE, H.I.; HECHT, T.; POTGEITER, B. 1996 The effect of four anesthetics on Haliotis midae and their suitability for application in commercial abalone culture. Aquaculture, 140(1-2): 145-151.
WOODALL, A.J.; NARUO, H.; PRINCE, D.J.; FENG, Z.P.; WINLOW, W.; TAKASAKI, M.; SYED, N.I. 2003 Anesthetic treatment blocks synaptogenesis but not neuronal regeneration of cultured Lymnaea neurons. Journal of Neurophysiology, 90(4): 2232-2239.
ZEIDAN, G.C. 2017 Efeito do tributil-estanho (TBT) sobre Stramonita rustica (Neogastropoda) e Littoraria angulifera (Mesogastropoda). Ilhéus (BA), 189p. (Tese de Doutorado. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz).
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2018-12-25
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Nota científica (Short Communication)
