Enriquecimento de Artemia sp. com probiótico autóctone em diferentes níveis na larvicultura de piauçu Megaleporinus macrocephalus
Palavras-chave:
Growth performance, Live food, Sanity, Microbiology, PathogenResumo
A pesquisa investigou o efeito da suplementação dietética com Artemia sp. enriquecida com o probiótico autóctoneEnterococcus faecium no desempenho do crescimento, modulação da microbiota, morfologia intestinal e resistência a bactérias patogênicas em larvas de Megaleporinus macrocephalus. O estudo avaliou quatro tratamentos (C: sem
probióticos; T1: 1 × 104; T2: 1 × 106; e T3: 1 × 108 UFC·mL-1) em quadruplicata. As larvas (n = 160; peso = 5,3 ± 2,3 mg e comprimento = 3,73 ± 0,4 mm) foram distribuídas em recipientes de 16 L na densidade de 10 larvas·L-1 por 20 dias. Desempenho produtivo, sobrevivência, microbiologia e histologia intestinal foram medidos. As larvas também foram submetidas a desafio agudo contra a bactéria patogênica Aeromonas hydrophila. Os resultados mostraram que a suplementação com 1 × 108 UFC·mL-1 promoveu maior ganho de comprimento (13,78 ± 0,40 cm) e peso total
(0,08 ± 0,002 g), maior contagem de bactérias lácticas e menor de heterotróficos totais nos intestinos (7,11 ± 0,30; 0,12 ± 0,09 log UFC·g-1, respectivamente) e maiores vilosidades (0,26 ± 0,03 μm). As dietas contendo probióticos influenciaram a resistência dos animais à infecção aguda, com menor mortalidade acumulada em T3 (33,33%±11,54%) e maior em C+ (93,33% ± 11,54%). Assim, a suplementação probiótica com a bactéria autóctone E. faecium (1 × 108 UFC·mL-1) proporciona melhora zootécnica, aumento de vilosidades e maior resistência a infecções.
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Copyright (c) 2023 Francisco Alex Lima Barros; Joel Artur Rodrigues Dias; Higo Andrade Abe, Keber Santos Costa Júnior, Natalino da Costa Sousa, Cordeiro, C.A.M., Rodrigo Yudi Fujimoto
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