Efeito do choque térmico quente em ovos de tilápia nilótica (Oreochromis niloticus): tempo pós-fertilização e duração do processo na sobrevivência das larvas
Palavras-chave:
Oreochromis niloticus, tetraploidia, choque térmico quente, macho monossexo, larvasResumo
A aplicação de choques térmicos quentes em ovos de tilápia-do-nilo, Oreochromis niloticus, recém fertilizados é empregada para bloquear a 2ª divisão meiótica ou inibir a 1ª clivagem, técnica essa aplicada para produzir populações monossexo, estéreis ou poliploides. Neste estudo foram realizados dois ensaios: 1º, utilizando tempos pós-fertilização (Tpf) de 50, 55 e 60 min e duração do choque térmico de 2 e 5 min.; e 2º, com tempos pós-fertilização de 24, 27 e 30 min e duração do choque de 5 e 8 min, em abril e dezembro de 2003, nos laboratórios do CAUNESP/UNESP, Jaboticabal, SP, Brasil. Somente as fêmeas foram induzidas í reprodução com HCG, resultando ser esse método eficiente na produção de gametas. Diferenças significativas (P < 0,05) entre os tratamentos (diferentes momentos de aplicação do choque térmico e tempos de duração do choque), nos dois ensaios, foram constatadas em relação í estimativa das taxas de sobrevivência das larvas. Porcentagens elevadas de animais deformados foram registradas. As baixas taxas de sobrevivência observadas, tanto nos grupos controle quanto nos tratamentos, indicam que a indução hormonal e a incubação (fertilização até eclosão) artificial afetam o desenvolvimento embrionário das larvas.
Referências
ARAI, K. 2001 Genetic improvement of Aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture, 197: 205-228.
BHUJEL, R.C. 2000 A review of strategies for the management of tilápia (Oreochromis niloticus) broodfish in seed production systems, especially hapa-based systems. Aquaculture, 181: 37-59.
BORGERS, A.B.J.; IN´T VELD, E.P.C.; ABOHASHEMA, K.; BREMMER, I.M.; EDING, E.H.; KOMEN, J.; RICHTER, C.J.J. 1994 Androgenesis in common carp (Cyprinus carpio L.) using UV irradiation in a synthetic ovarian fluid and heat shocks. Aquaculture, 122: 119-132.
BROWM, C.L. e NUí"˜EZ, J.M. 2003 Disorders of Development. Disponível em: www.cabi-publishin.org/Bookshop/ReardingRoom/ 08951991262/1262ch1.pdf (18/12/2003).
BRUZKA, E. 1979 The in vivo method of estimating the stages of oocytes maturation in carp (Cyprinus carpio L.). Acta Hydrobiol., 21: 423í 433.
BYAMUNGU, N.; DARRAS, V.M.; KUHN, E.R. 2001 Grow of heat shock induced triploids of blue tilápia, Oreochromis aureus, reared in tanks and in ponds in Eastern Congo: feeding regimes and compensatory growth response of triploid females. Aquaculture, 198: 109-122.
CARRILLO, M. e CHACÓN, M. 1995 Ensayo de androgénesis diploide en tilápia roja mediante manipulación cromosomal. Bogotá. 52p. (Tese de graduação. Universidade Jorge Tadeo Lozano).
CELIS, C. e MARTINEZ, H. 1993 Análisis de crecimiento y viabilidad entre monocultivos de poblaciones diploides y triploides de tilápia nilótica (O. niloticus). Bogotá. 152p. (Tese de Graduação.Universidade de Colômbia).
CONTRERAS, P. e CONTRERAS, J. 1993 Reproducción inducida de peces tropicales. In: RODRIGUEZ, H.; POLO, G.; SALAZAR, G. (Ed.). Fundamentos de Acuicultura Continental. 1. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA). 286p.
DON, J. e AVTALION, R.R. 1988 Production of viable tetraploidy tilápias using cold shock technique. Bamidgeh, 40: 17-21.
ESPEJO, C. e TORRES, E. 2001 Cultivo de las tilapias roja (Oreochromis spp.) y la plateada (Oreochromis niloticus). In: RODRÍGUEZ, H.; VICTORIA, P.; CARRILLO, M. (Ed.). Fundamentos de Acuicultura Continental. 2. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA). 423p.
EL GAMAL, A.A.; DAVIS, K.B.; JENKINS, J.A.; TORRANS, E.L. 1999 Induction of triploid and tetraploid in Nile tilápia, Oreochromis niloticus (L.). Journal of the World Aquaculture Society, 30: 269-275.
FELIP, A.; ZANUY, S.; CARRILLO, M.; PIFERRER, F. 1999 Growth and gonadal development in triploid sea bass (Dicentrarchus labrax) during the first two years of age. Aquaculture, 173: 389-399.
GUERRERO, R.D. III. 1982 Control of tilápia reproduction. In: PULLIN, R.S.V. e Mc CONNELL (Ed.). The biology and culture of tilápias. Manilla: ICLARM Conference Proceedings, 7. / International Center for Living Aquatic Resources Management. 432p.
HARVEY, B. e HOAR, W. 1980 Teoría y práctica de la reproducción inducida en los peces. Ottawa. 48p.
HERBST, E.C. 2002 Induction of tetraploidy in Zebrafish, Danio rerio and Nile Tilápia Oreochromis niloticus.
Carolina do Norte. 48p. (Dissertação de Mestrado. Universidade de North Carolina at Carlotte).
HUSSAIN, M.G.; PENMAN, D.J.; McANDREW, B.J.; JOHNSTONE, R. 1993 Supression of first cleavage in the Nile tilápia, Oreochromis niloticus L. í a comparison of the relative effectiveness of pressure and heat shock. Aquaculture,111: 263-270.
KARAYUCEL, I. e KARAYUCEL, S. 2003 Optimization of UV treatment duration to induced haploid androgenesis en the Nile tilápia (Oreochromis niloticus L.). J. Vet. Anim. Sci.,27: 401-407.
MAIR, G.C. 1993 Chromosome-set manipulation in tilápia í techniques, problems and prospects.Aquaculture, 111: 227-244.
MARENGONI, N.G. e ONOUE, Y. 1998 Ultravioletí Induced Androgénesis in Nile Tilápia,Oreochromis niloticus (L.), and hybrid Nile x blue tilápia, O. aureus (Steindachner). Aquaculture Research, 29: 359-366.
MAY, B.; HENLEY, K.J.; KRUEGER, C.C.; GLOSS, S.P. 1988 Androgenesis as a mechanism for chromosome set manipulation in brook trout (Salvelinus fontinalis ). Aquaculture, 75: 57-70.
MULLER-BELECKE A. e HORSTGEN-SCHWARK, G.S. 1995 Ex-determination in tilápia (Oreochromis niloticus). Sex ratio in homozygous gynogenetic progeny and their offspring.Aquaculture, 137: 57-65. 1995.
MYERS, J. 1986 Tetraploids induction in Oreochromis spp. Aquaculture, 57: 281-287.
MYERS, J.M.; PENMAN, D.J.; RANA, K.J.; BROMAGE, N.; POWELL, S.F.; McANDREW, B.J. 1995 Applications of induced androgenesis with tilápia. Aquaculture, 137: 149-160.
PANDIAN, T. e VARADARAJ, K. 1988. Techniques for producing all-male and all-triploid Oreochromis mossambicus. p. 243-249. In: PULLIN, R.S.V.; BHUKASWAN, T.; TONGUTHAI; K.;
MACLEAN, J.L. (Ed.). The second International Symposium on Tilapia in Aquaculture; ICLARM Conference Proceedings, 15. Manila: Department of fisheries, Bangkok, and International Center for Living Aquatic Resources Management.p.243-249.
RANA, K. 1988 Reproductive biology and hatchery rearing of tilapia eggs and fry. In: MUIR, J.F. e ROBERTS, R.J. (Ed.). v. 3: Recent Advances in Aquaculture. Colorado: Westview Press. p.343-406.
SHELTON, W.L. 1998 Artificial propagation tilápia for chromosome manipulation. In: BURKE, D.; BAKER, J.; GOETZE, B.; CLAIR, D.; EGNA, H. (Ed.). Oregon: Fifteenth Annual TechnicalReport. Pond Dynamics/Aquaculture. p.25-29.
SHELTON, W.L. 1999 Nile Tilápia Gamete Management for Chromosome Manipulation. In: BURKE, D.;BAKER, J.; GOETZE, B.; CLAIR, D.; EGNA, H. (Ed.). Oregon: Fifteenth Annual Technical Report. Pond Dynamics/Aquaculture. p.25-29.
SHELTON, W.L. 2000 Methods for androgenesis techniques applicable to tilapia. In: McELWEE, K.; BURQUE, D.; NILES, M.; EGNA, H. (Ed.). Oregon: Seventeenth Annual Technical Report.Pond Dynamics/Aquaculture. p.51-55.
SHELTON, W.L. 2002 Monosex Tilapia Production through Androgenesis. In: McELWEE, K.; LEWIS, K.; NIDIFFER, M.; BUITRAGO, P. (Ed.). Oregon: Nineteenth Annual Technical Report. Pond Dynamics/Aquaculture. p.1-9.
TORRES, E. 1993 El cultivo de la mojarra plateada (Oreochromis niloticus) y la mojarra roja (Oreochromis sp.). In: RODRÍGUEZ, H.; POLO, G.; SALAZAR, G. (Ed.). Fundamentos de Acuicultura Continental. 1. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA).p.193-205.
BHUJEL, R.C. 2000 A review of strategies for the management of tilápia (Oreochromis niloticus) broodfish in seed production systems, especially hapa-based systems. Aquaculture, 181: 37-59.
BORGERS, A.B.J.; IN´T VELD, E.P.C.; ABOHASHEMA, K.; BREMMER, I.M.; EDING, E.H.; KOMEN, J.; RICHTER, C.J.J. 1994 Androgenesis in common carp (Cyprinus carpio L.) using UV irradiation in a synthetic ovarian fluid and heat shocks. Aquaculture, 122: 119-132.
BROWM, C.L. e NUí"˜EZ, J.M. 2003 Disorders of Development. Disponível em: www.cabi-publishin.org/Bookshop/ReardingRoom/ 08951991262/1262ch1.pdf (18/12/2003).
BRUZKA, E. 1979 The in vivo method of estimating the stages of oocytes maturation in carp (Cyprinus carpio L.). Acta Hydrobiol., 21: 423í 433.
BYAMUNGU, N.; DARRAS, V.M.; KUHN, E.R. 2001 Grow of heat shock induced triploids of blue tilápia, Oreochromis aureus, reared in tanks and in ponds in Eastern Congo: feeding regimes and compensatory growth response of triploid females. Aquaculture, 198: 109-122.
CARRILLO, M. e CHACÓN, M. 1995 Ensayo de androgénesis diploide en tilápia roja mediante manipulación cromosomal. Bogotá. 52p. (Tese de graduação. Universidade Jorge Tadeo Lozano).
CELIS, C. e MARTINEZ, H. 1993 Análisis de crecimiento y viabilidad entre monocultivos de poblaciones diploides y triploides de tilápia nilótica (O. niloticus). Bogotá. 152p. (Tese de Graduação.Universidade de Colômbia).
CONTRERAS, P. e CONTRERAS, J. 1993 Reproducción inducida de peces tropicales. In: RODRIGUEZ, H.; POLO, G.; SALAZAR, G. (Ed.). Fundamentos de Acuicultura Continental. 1. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA). 286p.
DON, J. e AVTALION, R.R. 1988 Production of viable tetraploidy tilápias using cold shock technique. Bamidgeh, 40: 17-21.
ESPEJO, C. e TORRES, E. 2001 Cultivo de las tilapias roja (Oreochromis spp.) y la plateada (Oreochromis niloticus). In: RODRÍGUEZ, H.; VICTORIA, P.; CARRILLO, M. (Ed.). Fundamentos de Acuicultura Continental. 2. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA). 423p.
EL GAMAL, A.A.; DAVIS, K.B.; JENKINS, J.A.; TORRANS, E.L. 1999 Induction of triploid and tetraploid in Nile tilápia, Oreochromis niloticus (L.). Journal of the World Aquaculture Society, 30: 269-275.
FELIP, A.; ZANUY, S.; CARRILLO, M.; PIFERRER, F. 1999 Growth and gonadal development in triploid sea bass (Dicentrarchus labrax) during the first two years of age. Aquaculture, 173: 389-399.
GUERRERO, R.D. III. 1982 Control of tilápia reproduction. In: PULLIN, R.S.V. e Mc CONNELL (Ed.). The biology and culture of tilápias. Manilla: ICLARM Conference Proceedings, 7. / International Center for Living Aquatic Resources Management. 432p.
HARVEY, B. e HOAR, W. 1980 Teoría y práctica de la reproducción inducida en los peces. Ottawa. 48p.
HERBST, E.C. 2002 Induction of tetraploidy in Zebrafish, Danio rerio and Nile Tilápia Oreochromis niloticus.
Carolina do Norte. 48p. (Dissertação de Mestrado. Universidade de North Carolina at Carlotte).
HUSSAIN, M.G.; PENMAN, D.J.; McANDREW, B.J.; JOHNSTONE, R. 1993 Supression of first cleavage in the Nile tilápia, Oreochromis niloticus L. í a comparison of the relative effectiveness of pressure and heat shock. Aquaculture,111: 263-270.
KARAYUCEL, I. e KARAYUCEL, S. 2003 Optimization of UV treatment duration to induced haploid androgenesis en the Nile tilápia (Oreochromis niloticus L.). J. Vet. Anim. Sci.,27: 401-407.
MAIR, G.C. 1993 Chromosome-set manipulation in tilápia í techniques, problems and prospects.Aquaculture, 111: 227-244.
MARENGONI, N.G. e ONOUE, Y. 1998 Ultravioletí Induced Androgénesis in Nile Tilápia,Oreochromis niloticus (L.), and hybrid Nile x blue tilápia, O. aureus (Steindachner). Aquaculture Research, 29: 359-366.
MAY, B.; HENLEY, K.J.; KRUEGER, C.C.; GLOSS, S.P. 1988 Androgenesis as a mechanism for chromosome set manipulation in brook trout (Salvelinus fontinalis ). Aquaculture, 75: 57-70.
MULLER-BELECKE A. e HORSTGEN-SCHWARK, G.S. 1995 Ex-determination in tilápia (Oreochromis niloticus). Sex ratio in homozygous gynogenetic progeny and their offspring.Aquaculture, 137: 57-65. 1995.
MYERS, J. 1986 Tetraploids induction in Oreochromis spp. Aquaculture, 57: 281-287.
MYERS, J.M.; PENMAN, D.J.; RANA, K.J.; BROMAGE, N.; POWELL, S.F.; McANDREW, B.J. 1995 Applications of induced androgenesis with tilápia. Aquaculture, 137: 149-160.
PANDIAN, T. e VARADARAJ, K. 1988. Techniques for producing all-male and all-triploid Oreochromis mossambicus. p. 243-249. In: PULLIN, R.S.V.; BHUKASWAN, T.; TONGUTHAI; K.;
MACLEAN, J.L. (Ed.). The second International Symposium on Tilapia in Aquaculture; ICLARM Conference Proceedings, 15. Manila: Department of fisheries, Bangkok, and International Center for Living Aquatic Resources Management.p.243-249.
RANA, K. 1988 Reproductive biology and hatchery rearing of tilapia eggs and fry. In: MUIR, J.F. e ROBERTS, R.J. (Ed.). v. 3: Recent Advances in Aquaculture. Colorado: Westview Press. p.343-406.
SHELTON, W.L. 1998 Artificial propagation tilápia for chromosome manipulation. In: BURKE, D.; BAKER, J.; GOETZE, B.; CLAIR, D.; EGNA, H. (Ed.). Oregon: Fifteenth Annual TechnicalReport. Pond Dynamics/Aquaculture. p.25-29.
SHELTON, W.L. 1999 Nile Tilápia Gamete Management for Chromosome Manipulation. In: BURKE, D.;BAKER, J.; GOETZE, B.; CLAIR, D.; EGNA, H. (Ed.). Oregon: Fifteenth Annual Technical Report. Pond Dynamics/Aquaculture. p.25-29.
SHELTON, W.L. 2000 Methods for androgenesis techniques applicable to tilapia. In: McELWEE, K.; BURQUE, D.; NILES, M.; EGNA, H. (Ed.). Oregon: Seventeenth Annual Technical Report.Pond Dynamics/Aquaculture. p.51-55.
SHELTON, W.L. 2002 Monosex Tilapia Production through Androgenesis. In: McELWEE, K.; LEWIS, K.; NIDIFFER, M.; BUITRAGO, P. (Ed.). Oregon: Nineteenth Annual Technical Report. Pond Dynamics/Aquaculture. p.1-9.
TORRES, E. 1993 El cultivo de la mojarra plateada (Oreochromis niloticus) y la mojarra roja (Oreochromis sp.). In: RODRÍGUEZ, H.; POLO, G.; SALAZAR, G. (Ed.). Fundamentos de Acuicultura Continental. 1. ed. Bogotá: Instituto Nacional de Pesca y Acuicultura (INPA).p.193-205.
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2018-07-19
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