Suplementação de prebióticos, probióticos e algas marinhas na dieta de tilápia juvenil
DOI:
https://doi.org/10.20950/1678-2305/bip.2024.50.e819Palavras-chave:
Immunostimulants, Symbiotics, Nutrition, Hematology, Aeromonas hydrophila, AquacultureResumo
O objetivo foi avaliar o uso de probióticos, mananoligossacarídeos (MOS) e algas marinhas na alimentação de tilápia do Nilo (Oreochromis niloticus), analisando-se a taxa de crescimento, os parâmetros hematológicos e imunológicos e a microbiota intestinal. Além disso, foram submetidos à infecção com Aeromonas hydrophila para avaliar a resposta imune. Tilápia do Nilo (peso 8,86 ± 3,22 g) foram utilizados nos seis tratamentos com quatro repetições, durante 63 dias. Os tratamentos foram: dieta basal; PAS-TR: dieta basal mais 0,04 g·kg-1 do probiótico (Bacillus cereus e Bacillus subtilis); MOS: dieta basal acrescida de 4,0 g·kg-1 do prebiótico manooligossacarídeo, 4,0 g·kg-1 do prebiótico Kappaphycus alvarezii. Duas dietas foram formuladas com PAS-TR + MOS (4,0 +4,0 g·kg-1) e PAS-TR + KAP (4,0 +4,0 g·kg-1). Para o desafio, foram alimentados por 21 dias, infectados via injeção intraperitoneal com A. hydrophila (1 x 106 UFC mL-1), e a taxa de mortalidade registrada por 15 dias pós-infecção. Os resultados indicaram a capacidade do probiótico permanecer no intestino por 63 dias, e não foi inibido pela microbiota autóctone nem pelos prebióticos. Os aditivos alimentares testados não causaram efeito benéfico ou adverso no crescimento ou nas variáveis hematológicas avaliadas. Mas os tratamentos protegeram os peixes da infecção por A. hydrophyla. Concluímos que o probiótico PAS-TR e os prebióticos MOS e KAP, combinados ou não como simbióticos, podem promover proteção imunológica e reduzir a taxa de mortalidade da infecção por A. hydrophyla.
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Copyright (c) 2024 Maria José T. Ranzani-Paiva, Danielle de Carla Dias, Raissa Bertoncello Cavalcante, Guilherme Telli, Eliana Oshiro, Carlos Massatoshi Ishikawa, Maria Letizia Petesse, Miguel Frederico Fernandez-Alarcon, Mariene Miyoko Natori, Miguel Angel Moriñigo, Silvana Tapia, Said Ben Hamed, Leonardo Tachibana
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