Bacterial density and coliform organisms in waters and oysters of Paranaguá estuarine complex, Paraná, Brazil
Keywords:
oysters, water, coliform bacteria, Paranaguá Estuarine ComplexAbstract
The objective of the present research was to evaluate quantitatively the total coliforms and E. coli in oysters and adjacent waters of the Paranaguá Estuarine Complex and its interrelation with biotic and abiotic factors, as well as evaluate the contamination from coliforms of oysters sold in the Paranaguá market. Water and oysters were collected from April of 1997 to February of 1998, in the Paranaguá Estuarine Complex and oysters were bought from the same three merchants in the market throughout the research. Temperature, salinity and seston of the water and total heterotrophic bacteria, bacterial biomass, total coliforms and Escherichia coli in the water and in the oysters were analyzed. Temperature was higher in summer, and salinity and seston in the winter. Total heterotrophic bacteria and bacterial biomass in the water were heigher in Ostras River in July and January and total coliforms, in July. The highest values of total coliforms in oysters were observed in Rasa Island, in January. In the water, the number of E. coli was larger than 2,419 MPN.100 mL-1 in Cobras’ Island in April and in Puruquara in July. In relation to the oysters from the market of Paranaguá, the largest values of total coliforms were registered in April and December, and E. coli in December, both from merchant 2. The results show that oysters collected in the environment as well as those from the Municipal Market of Paranaguá cannot be consumed raw without previous purification and that there is a need of an urgent reform of the legislation, including the analysis of E. coli in the oysters to be marketed.
References
larval, recrutamento e crescimento. São Paulo. 185p. (Tese de Doutoramento. Universidade de São Paulo, Instituto Oceanográfico).
ANVISA (Agência Nacional de Vigilí¢ncia Sanitária). 2001 Resolução - RDC nº 12, de 2 de janeiro de 2001. Disponível em: http://www.anvisa.gov.br/legis/resol/12_01rdc.htm. Acesso em: 10 de jul. de 2007.
BJíËœRNSEN, P.K.; KUPARINEN, J. 1991 Determination of bacterioplankton biomass, net production and growth efficiency in the Southern Ocean. Mar. Ecol. Prog. Ser., 71: 185-194.
BRISOU, J. 1974 Infections et intoxications transmises par les coquillages. Quest. Méd., 27: 1321-1329.
BURKHADT, W.; CALCII, K.R. 2000 Selective accumulation may account for shellfish-associated viral illness. Appl. Environ. Microbiol., 66(4): 1375-1378.
CLAYTON, W.E.L. 2006 Microbial Source TrackingThe use of new research tools for the identification and tracking of bacterial pollution sources affecting shellfish culture opportunities. PublishedAquaculture Update # 97, August 2007. Marine Ecosystems and Aquaculture Division, Fisheries and Oceans Canadá. Disponível em: http://www.ieccorporate.com/publications.html.
CONAMA (Conselho Nacional do Meio Ambiente, dos Recursos Hídricos e da Amazônia Legal). 2005 Resolução no . 357/05 de 17 de março de 2005. Livro de resoluções do CONAMA. p. 274. Disponível em: http://mma.gov.br/port/conama/index.cfm.Acesso em: 10 de jul 2007.
ESCOBAR NIEVES, A. 1988 Condicion bacteriologica de las ostras del manglar (Crassostrea rhizophorae
Guilding) en la Cienaga Grande de Santa Marta, Caribe Colombiano. An. Inst. Inv. Mar. Punta de Betín., 18: 137-151.
GALTSOFF, P.S. 1964 American Oyster, Crassostrea virginica (Gmelin). Fishery Bull. natn. mar. Fish Serv., U.S., 64: 1-430.
HOOD, M.A.; NESS, G.E. 1982 Survival of Vibrio cholerae and Escherichia coli in Estuarine Waters
and Sediments. Appl. Environ. Microbiol., 43(3):578-584.
KINNE, O. 1983 Diseases of marine animals. Vol. II. Biologische Anstalt Helgoland. Hamburg. 1038p.
MAYERLE FARIA, J. 2002 Estudo da qualidade bacteriológica das águas e das ostras (Crassostrea gigas) cultivadas na Baía da Babitonga e sua adequação para fins de comercialização e consumo. Joinville. 68p.
(Dissertação de Mestrado. Universidade da Região de Joinville).
METCALF, T.G.; MULLIN, B.; ECKERSON, D.; MOULTON, E.; LARKIN, E.P. 1979 ioaccumulation and depuration of enteroviruses by the soft-shelled clam, Mya arenaria. Appl. Environ. Microbiol.,38: 275-282.
PARSONS, T.R.; MAITA,Y.; LALLI,C.M. 1984 Direct Counting of Bacteria by Fluorescence Microscopy. In: A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press. 173p.
PEREIRA, M.A.; NUNES, M.M.; NUERNBERG, L.; SCHULZ, D.; BATISTA, C.R.V. 2006 Microbiological quality of oysters (Crassostrea gigas) produced and commercialized in the coastal region of Florianópolis í Brazil. Braz. J. Microbiol.
37: 159-163.
POMMEPUY, M.; UTIN, M.B.; DERRIER, A.; GOURMELON, M.; COLWELL, R.R.; CORMIER, M. 1996 Retention of enteropathogenicity by variable but nonculturable Escherichia coli exposed to seawater and sunligh. Appl. Environ.Microbiol.,62(12): 4621-4626.
REBELLO J., BRANDINI F. P. 1990 Variação temporal de parí¢metros hidrográficos e material particulado
em suspensão em dois pontos fixos da Baía de Paranaguá, Paraná (junho/87-fevereiro/88). Nerítica, 5: 95-111.
RIPPEY, S.R. 1994 Infectious diseases associated with molluscan shellfish consumption. Clin. Microbiol.Rev. 7: 419-425.
SILVA, A.I.M.; VIEIRA, R.H.S.F.; MENEZES, F.G.R.; FONTELES-FILHO, A.A.; TORRES, R.C.O.; SANT´ANA, E.S. 2003 Bacteria of fecal origin in mangrove oysters (Crassostrea rhizophorae) in the Cocó river estuary, Ceará state, Brazil. Braz. J.Microbiol. 34: 126-130.
SIQUEIRA, A.; KOLM, H.E. 2005 Bacterioplí¢ncton na desembocadura da Gamboa do Maciel, Baía de Paranaguá, Paraná, Brasil. Revista Saúde e Ambiente, 6(1): 20-28.
VILLALOBOS, L.B.; ELQUEZABAL, L. 2000 Detección de posible Escherichia coli enteropatógena en el bivalvo Pinctada imbricata comercializado en Cumaná, Venezuela. Bol. Inst. Oceanogr. Venezuela. 39: 17-23.
