Refrigerated storage of lane snapper Lutjanus synagris sperm
Keywords:
refrigerated sperm, reproduction, maricultureAbstract
The aim of this study was to develop a protocol to refrigerated storage of the lane snapper sperm. Firstly, motility rate, motility time, sperm density and spermatocrit were appraised to characterize the fresh sperm quality. Three different extenders with different ionic compositions and pH values were used in the refrigerated storage sperm at 4°C in normal atmosphere and modified atmosphere (100% oxygen). Fertilization test was accomplished to evaluate the viability of the refrigerated sperm. The results demonstrated that the fresh sperm has a spermatozoa density of 2.2 ± 0.2 x 109 spermatozoa mL-1, motility rate >90% and motility time for 137 ± 20 seconds. A positive relationship was found between spermatocrit and spermatozoa density (r2 = 0.75; P<0.05). In the experiment of refrigerated storage, the motility rate and the motility time were maintained appropriate for 72 hours for the extender A (63 ± 6%; 274 ± 44 seconds) and B (63 ± 6%; 172 ± 7 seconds) in normal atmosphere. In the modified atmosphere, the sperm quality fell drastically during the first 24 hours, independently of the used extender, not propitiating advantage. The results demonstrated that the refrigerated sperm of lane snapper is a viable alternative for the short-term storage, being possible to maintain for up to 48 hours an appropriate sperm viability, providing fertilization rate up to 50%.
References
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Persian sturgeon: effects of pH, dilution rate, ions and osmolality. Reproduction Research,Baton Rouge, 128(1): 819-828.
BENCIC, D.C.; KRISFALUSI, M.; CLOUD, J.G.; INGERMANN, R.L. 2000 Short-term storage of salmonid sperm in air versus oxygen. North American Journal of Aquaculture, New York,62(1): 19-25.
BILLARD, R.; COSSON, S.B.; NOVIERI M.;POURKAZEMI, M. 2004 Cryopreservation
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CARNEIRO, P.C.F; SEGUI, M.S.; IÓRIS FILHO,C.R.; MIKOS, J.D. 2006 Viabilidade do sêmen de jundiá, Rhamdia quelen armazenado sob refrigeração. Revista Acadêmica, Belo Horizonte, 4(3): 11-16.
CHEREGUINI, O.; CAL, R.M.; DREANNO, C.; BAULNY, B.O.; SUQUET, M.; MAISSE, G.
1997 Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm.Aquatic Living Resources, Paris, 10: 251-255.
CHEREGUINI, O.; BANDA, I.G. DE LA; RASINES,I.; FERNANDEZ, A. 2001 Larval growth of turbot, Scophthalmus maximus (L.) produced with fresh and cryopreserved sperm. Aquaculture Reserch, Oxford, 32(1): 133-143.
CLARO, R. e LINDEMAN, K.C. 2008 Biología y manejo de los pargos (Lutjanidae) en el Atlántico occidental. Havana: Instituto de Oceanología/CITMA. 472 p.
CRUZ-CASALLAS, P.E.; VELASCOSANTAMARIA, Y.M.; MEDINA-ROBLES,V.M. 2006 Determinación del espermatocrito y efecto del volumen de la dosis seminante
sobre la fertilidad en yamú (Brycon amazonicus). Revista Colombiana de Ciencias
Pecuarias, Bogotá, 19(2): 140-145.
DILAURO, M.N., KRISE, W.F., HENDRIX, M.A., BAKER, S.E. 1994 Short term storage of
Atlantic sturgeon sperm. The Progressive FishCulturist, New York, 56(1): 143-144.
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GARZÓN, D.L.; PEí"˜ARANDA, D.S.; PÉREZ, L.;MARCO-JIMÉNEZ, F.; ESPERT, X.; MíÅ“LLER
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GODINHO, H.P. 2000 Criopreservação de sêmen de peixes. Informe Agropecuário, Belo Horizonte, 21(3): 16-20.
GWO, J.C. 2000 Cryopreservation of sperm of some marine fishes. In: TIERSCH, T.R. e
MAZIK, P.M. Cryopreservation in Aquatic Species. Baton Rouge: World Aquaculture
Society. p.138-160.
INABA, K. 2003 Molecular architecture of the sperm flagella: molecules for motility and signaling. Zoological Science, Londres, 20: 1043-1056.
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LAHNSTEINER, F., WEISMANN, T.; PATZNER,R.A. 1997 Methanol as cryoprotectant and the suitability of 1,2ml and 5ml strans for cryopreservation of semen from salmonid
fishes. Aquaculture Research, Oxford, 28(2):471-479.
LANES, C.F.C.; OKAMOTO, M.; CAVALCANTI,P.V.; COLARES, T.; CAMPOS, V.F.;
DESCHAMPS, J.C.; ROBALDO, R.B.; MARINS, L.F.; SAMPAIO, L.A. 2008 Cryopreservation of Brazilian flounder (Paralichthys orbignyanus) sperm. Aquaculture,Amsterdam, 275(1): 361-365.
MARQUES, S. e GODINHO, H.P. 2004 Short-term cold storage of sperm from six tropical characiformes fishes. Brazialian Archives of Biology and Technology, Maringá, 47(5): 799-804.
MUCHLISIN, Z.A. 2005 Current Status of Extenders and cryoprotectants on fish
spermatozoa cryopreservation. Biodiversitas,Halamn, 6(1): 66-69.
ORFÃO, L.H.; MARIA, A.N.; NASCIMENTO,A.F.; ISAíÅ¡, Z.A.; VIVEIROS, A.T.M. 2010
Sperm fertility of the subtropical freshwater streaked prochilod Prochilodus lineatus
Characiformes) improved after dilution and cold storage. Aquaculture Research, Oxford, 41:679-687.
ODA, S. e MORISAWA, M. 1993 Rises of intracellular Ca+ and pH mediate the
initiation of sperm of farmed european eel Anguilla anguilla. Journal of World Aquaculture Society, Baton Rouge, 35: 240-246.
OLIVEIRA, A.V.; VIVEIROS, A.T.M.; MARIA, A.N. FREITAS, R.T.F.; IZAíÅ¡, Z.A. 2007
Sucesso do resfriamento e congelamento do sêmen de pirapitinga Brycon nattereri. Arquivo Brasileiro de Medicina Veterinária e Zootecnia,Belo Horizonte, 59(6): 1509-1515.
PEí"˜ARANDA, D.S.; PÉREZ, L.; GALLEGO, V.;BARRERA, R.; JOVER, M.; ASTURIANO, J.F.
2010 European eel sperm diluent for shortterm storage. Reproduction in Domestic
Animals, Madri, 45: 407-415.
REZENDE, S.M.; FERREIRA, B.P.; FREDOU, T.2003 A pesca de lutjanídeos no nordeste do Brasil: histórico das pescarias, características das espécies e releví¢ncia para o manejo. Boletim Técnico Cientifico do CEPENE, Recife,11: 1-17.
RILEY, K.L.; HOLLADAY, C.G.; CHESNEY, E.J.; TIERSCH, T.R. 2004 Cryopreservation of
sperm of red snapper Lutjanus campechanus. Aquaculture, Amsterdam, 238(1): 183-194.
RITAR, A.J. e CAMPET, M. 2000 Sperm survival during short-term storage and after
cryopreservation of semen from striped trupeter (Latris lineata). Theriogenology,
Amsterdam, 54(1): 467-480.
SAAD, A.; BILLARD, R.; THERON, M.C.;HOLLEBECQ, M.G. 1988 Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture, Amsterdam, 71(1): 133-150.
SANCHES, E.G.; HENRIQUES, M.B.;FAGUNDES, L.; SILVA, A.A. 2006 Viabilidade econômica do cultivo da garoupa verdadeira (Epinephelus marginatus) em tanques-rede, região sudeste do Brasil.Informações Econômicas, São Paulo, 36(8): 15-25.
SANCHES, E.G. 2007 Piscicultura marinha no Brasil: uma alternativa de produção e
conservação. Aquicultura e Pesca, São Paulo,36: 16-22.
SANCHES, E.G.; OLIVEIRA, I.R.;SERRALHEIRO, P.C. 2009 Crioconservação do sêmen da garoupa-verdadera Epinephelus marginatus (Lowe, 1834) (Teleostei,Serranidae). Boletim do Instituto de Pesca, São Paulo, 35(3): 389-399.
SAS INSTITUTE INC. 1990 SAS technical report.Release 6.11. Cary: NC. 229p.
STOSS, J. 1983 Fish gamete preservation and spermatozoan physiology. In: HOAR, W.S.; RANDALL, D.J.; DONALDSON, E.M. (Eds.).Fish physiology. New York: Academic Press.p.305-350.
SUQUET, M., DREANNO, C.; FAUVEL, C.,COSSON, J.; BILLARD, R. 2000
Cryopreservation of sperm in marine fish.Aquaculture Research, Oxford, 31(1): 231-243.
TANAKA, S.; ZHANG, H.; YAMADA, Y.;OKAMURA, A.; HORIE, N.; UTOH, T.,
MIKAWA, N.; OKA, H.P.; KUROKURA, H. 2002 Inibitory effect of sodium bicarbonate on the motility of sperm of Japanese eel. Journal of Fish Biology, Londres, 60: 139-146.
TIBA, R.M.; OLIVEIRA, I.R.; SERRALHEIRO,P.C.S.; OSTINI, S. 2009 Diluentes e proporções sêmen:diluente na crioconservação do sêmen do robalo-peva Centropomus parallelus. Boletim do Instituto de Pesca, São Paulo, 35(1): 99-110.
TIERSCH, T.R.; WAYMAN, W.R.; SKAPURA,D.P.; NEIDIG, C.L.; GRIER, H.J. 2004
Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Block). Aquaculture Research, Oxford, 35(1):278-288.
VUTHIPHANDCHAI, V.; CHOMPHUTHAWACH,S.; NIMRAT, S. 2009 Cryopreservation of red snapper Lutjanus argentimaculatus sperm:effect of cryoprotectants and cooling rates on sperm motility, sperm viability and fertilization capacity. Theriogenology,
Amsterdam, 72(1): 129-138.
WAYMAN, W.R.; THOMAS, R.G. TIERSCH, T.R.1997 Refrigerated storage and
cryopreservation of black drum Pogonias cromis spermatozoa. Theriogenology,
Amsterdam, 47(1): 1519-1529.
WAYMAN, W.R. e TIERSCH, T.R. 1998 Refrigerated storage and cryopreservation of
sperm of red drum, Sciaenops ocellatus L. Aquaculture Research, Oxford, 29(1): 267-273.
ZAR, J.H. 1999 Biostatistical analysis. USA: Ed.Prentice Hall. 929 p.
ALAVI, S.M.H.; COSSON, J.; KARAMI, M.; AMIRI, B.M. 2004 Spermatozoa motility in the
Persian sturgeon: effects of pH, dilution rate, ions and osmolality. Reproduction Research,Baton Rouge, 128(1): 819-828.
BENCIC, D.C.; KRISFALUSI, M.; CLOUD, J.G.; INGERMANN, R.L. 2000 Short-term storage of salmonid sperm in air versus oxygen. North American Journal of Aquaculture, New York,62(1): 19-25.
BILLARD, R.; COSSON, S.B.; NOVIERI M.;POURKAZEMI, M. 2004 Cryopreservation
and short-term storage of sturgeon: a review.Aquaculture, Amsterdam, 236(1): 1-9.
CARNEIRO, P.C.F; SEGUI, M.S.; IÓRIS FILHO,C.R.; MIKOS, J.D. 2006 Viabilidade do sêmen de jundiá, Rhamdia quelen armazenado sob refrigeração. Revista Acadêmica, Belo Horizonte, 4(3): 11-16.
CHEREGUINI, O.; CAL, R.M.; DREANNO, C.; BAULNY, B.O.; SUQUET, M.; MAISSE, G.
1997 Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm.Aquatic Living Resources, Paris, 10: 251-255.
CHEREGUINI, O.; BANDA, I.G. DE LA; RASINES,I.; FERNANDEZ, A. 2001 Larval growth of turbot, Scophthalmus maximus (L.) produced with fresh and cryopreserved sperm. Aquaculture Reserch, Oxford, 32(1): 133-143.
CLARO, R. e LINDEMAN, K.C. 2008 Biología y manejo de los pargos (Lutjanidae) en el Atlántico occidental. Havana: Instituto de Oceanología/CITMA. 472 p.
CRUZ-CASALLAS, P.E.; VELASCOSANTAMARIA, Y.M.; MEDINA-ROBLES,V.M. 2006 Determinación del espermatocrito y efecto del volumen de la dosis seminante
sobre la fertilidad en yamú (Brycon amazonicus). Revista Colombiana de Ciencias
Pecuarias, Bogotá, 19(2): 140-145.
DILAURO, M.N., KRISE, W.F., HENDRIX, M.A., BAKER, S.E. 1994 Short term storage of
Atlantic sturgeon sperm. The Progressive FishCulturist, New York, 56(1): 143-144.
FIGUEIREDO, J.L. e MENEZES, N.A. 2000 Manual de peixes marinhos do Sudeste do Brasil. São Paulo: Museu de Zoologia/USP. 116p.
GARZÓN, D.L.; PEí"˜ARANDA, D.S.; PÉREZ, L.;MARCO-JIMÉNEZ, F.; ESPERT, X.; MíÅ“LLER
T.; JOVER, M.; ASTURIANO, J.F. 2008 Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of european eel sperm.Reproduction in Domestic Animals, Madri, 43:99-105.
GODINHO, H.P. 2000 Criopreservação de sêmen de peixes. Informe Agropecuário, Belo Horizonte, 21(3): 16-20.
GWO, J.C. 2000 Cryopreservation of sperm of some marine fishes. In: TIERSCH, T.R. e
MAZIK, P.M. Cryopreservation in Aquatic Species. Baton Rouge: World Aquaculture
Society. p.138-160.
INABA, K. 2003 Molecular architecture of the sperm flagella: molecules for motility and signaling. Zoological Science, Londres, 20: 1043-1056.
KLIPPEL, S. e PERES, M.B. 2002 Resultados da avaliação de estoques das dez principais espécies na pesca de linha de mão da costa central do Brasil. Rio Grande:Programa REVIZEE. 60p.
LAHNSTEINER, F., WEISMANN, T.; PATZNER,R.A. 1997 Methanol as cryoprotectant and the suitability of 1,2ml and 5ml strans for cryopreservation of semen from salmonid
fishes. Aquaculture Research, Oxford, 28(2):471-479.
LANES, C.F.C.; OKAMOTO, M.; CAVALCANTI,P.V.; COLARES, T.; CAMPOS, V.F.;
DESCHAMPS, J.C.; ROBALDO, R.B.; MARINS, L.F.; SAMPAIO, L.A. 2008 Cryopreservation of Brazilian flounder (Paralichthys orbignyanus) sperm. Aquaculture,Amsterdam, 275(1): 361-365.
MARQUES, S. e GODINHO, H.P. 2004 Short-term cold storage of sperm from six tropical characiformes fishes. Brazialian Archives of Biology and Technology, Maringá, 47(5): 799-804.
MUCHLISIN, Z.A. 2005 Current Status of Extenders and cryoprotectants on fish
spermatozoa cryopreservation. Biodiversitas,Halamn, 6(1): 66-69.
ORFÃO, L.H.; MARIA, A.N.; NASCIMENTO,A.F.; ISAíÅ¡, Z.A.; VIVEIROS, A.T.M. 2010
Sperm fertility of the subtropical freshwater streaked prochilod Prochilodus lineatus
Characiformes) improved after dilution and cold storage. Aquaculture Research, Oxford, 41:679-687.
ODA, S. e MORISAWA, M. 1993 Rises of intracellular Ca+ and pH mediate the
initiation of sperm of farmed european eel Anguilla anguilla. Journal of World Aquaculture Society, Baton Rouge, 35: 240-246.
OLIVEIRA, A.V.; VIVEIROS, A.T.M.; MARIA, A.N. FREITAS, R.T.F.; IZAíÅ¡, Z.A. 2007
Sucesso do resfriamento e congelamento do sêmen de pirapitinga Brycon nattereri. Arquivo Brasileiro de Medicina Veterinária e Zootecnia,Belo Horizonte, 59(6): 1509-1515.
PEí"˜ARANDA, D.S.; PÉREZ, L.; GALLEGO, V.;BARRERA, R.; JOVER, M.; ASTURIANO, J.F.
2010 European eel sperm diluent for shortterm storage. Reproduction in Domestic
Animals, Madri, 45: 407-415.
REZENDE, S.M.; FERREIRA, B.P.; FREDOU, T.2003 A pesca de lutjanídeos no nordeste do Brasil: histórico das pescarias, características das espécies e releví¢ncia para o manejo. Boletim Técnico Cientifico do CEPENE, Recife,11: 1-17.
RILEY, K.L.; HOLLADAY, C.G.; CHESNEY, E.J.; TIERSCH, T.R. 2004 Cryopreservation of
sperm of red snapper Lutjanus campechanus. Aquaculture, Amsterdam, 238(1): 183-194.
RITAR, A.J. e CAMPET, M. 2000 Sperm survival during short-term storage and after
cryopreservation of semen from striped trupeter (Latris lineata). Theriogenology,
Amsterdam, 54(1): 467-480.
SAAD, A.; BILLARD, R.; THERON, M.C.;HOLLEBECQ, M.G. 1988 Short-term preservation of carp (Cyprinus carpio) semen. Aquaculture, Amsterdam, 71(1): 133-150.
SANCHES, E.G.; HENRIQUES, M.B.;FAGUNDES, L.; SILVA, A.A. 2006 Viabilidade econômica do cultivo da garoupa verdadeira (Epinephelus marginatus) em tanques-rede, região sudeste do Brasil.Informações Econômicas, São Paulo, 36(8): 15-25.
SANCHES, E.G. 2007 Piscicultura marinha no Brasil: uma alternativa de produção e
conservação. Aquicultura e Pesca, São Paulo,36: 16-22.
SANCHES, E.G.; OLIVEIRA, I.R.;SERRALHEIRO, P.C. 2009 Crioconservação do sêmen da garoupa-verdadera Epinephelus marginatus (Lowe, 1834) (Teleostei,Serranidae). Boletim do Instituto de Pesca, São Paulo, 35(3): 389-399.
SAS INSTITUTE INC. 1990 SAS technical report.Release 6.11. Cary: NC. 229p.
STOSS, J. 1983 Fish gamete preservation and spermatozoan physiology. In: HOAR, W.S.; RANDALL, D.J.; DONALDSON, E.M. (Eds.).Fish physiology. New York: Academic Press.p.305-350.
SUQUET, M., DREANNO, C.; FAUVEL, C.,COSSON, J.; BILLARD, R. 2000
Cryopreservation of sperm in marine fish.Aquaculture Research, Oxford, 31(1): 231-243.
TANAKA, S.; ZHANG, H.; YAMADA, Y.;OKAMURA, A.; HORIE, N.; UTOH, T.,
MIKAWA, N.; OKA, H.P.; KUROKURA, H. 2002 Inibitory effect of sodium bicarbonate on the motility of sperm of Japanese eel. Journal of Fish Biology, Londres, 60: 139-146.
TIBA, R.M.; OLIVEIRA, I.R.; SERRALHEIRO,P.C.S.; OSTINI, S. 2009 Diluentes e proporções sêmen:diluente na crioconservação do sêmen do robalo-peva Centropomus parallelus. Boletim do Instituto de Pesca, São Paulo, 35(1): 99-110.
TIERSCH, T.R.; WAYMAN, W.R.; SKAPURA,D.P.; NEIDIG, C.L.; GRIER, H.J. 2004
Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Block). Aquaculture Research, Oxford, 35(1):278-288.
VUTHIPHANDCHAI, V.; CHOMPHUTHAWACH,S.; NIMRAT, S. 2009 Cryopreservation of red snapper Lutjanus argentimaculatus sperm:effect of cryoprotectants and cooling rates on sperm motility, sperm viability and fertilization capacity. Theriogenology,
Amsterdam, 72(1): 129-138.
WAYMAN, W.R.; THOMAS, R.G. TIERSCH, T.R.1997 Refrigerated storage and
cryopreservation of black drum Pogonias cromis spermatozoa. Theriogenology,
Amsterdam, 47(1): 1519-1529.
WAYMAN, W.R. e TIERSCH, T.R. 1998 Refrigerated storage and cryopreservation of
sperm of red drum, Sciaenops ocellatus L. Aquaculture Research, Oxford, 29(1): 267-273.
ZAR, J.H. 1999 Biostatistical analysis. USA: Ed.Prentice Hall. 929 p.
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2018-11-08
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