Analysis of genetic diversity of farm-raised tilapia stocks from Santa Catarina state (Brazil) using microsatellite markers
Keywords:
finfish culture, genetic improvement, molecular markersAbstract
Three varieties of tilapia farmed at Panamá-Unisul Finfish Culture Station in Santa Catarina State were analyzed in order to evaluate the genetic diversity through microsatellites analysis. Genetic and genotypic differentiation, as well as, the degree of genetic divergence from all populations (FST) were significant (P<0.01). Using FST pairwise analysis from all populations, it was observed that the GIFT population had the highest divergence when compared to the VERMELHA (VERM) (FST = 0.11) and the PANAMÁ (PAN) (FST = 0.18). The GIFT variety (FIS = 0.16) presented the lower inbreeding coefficient when compared to the local varieties PAN (FIS = 0.33) and VERM (FIS = 0.28), showing that this variety had controlled management. The results obtained in this study evidence the importance of controlled management in farmed tilapias in order to maintain the genetic diversity of populations.
References
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Oreochromis niloticus, Oreochromis mossambicus and their hybrids. Aquaculture, 247: 189-201.
AGNESSE, J.; ADEPO GOURENE, B.; ABBAN, E.K.; FERMOSN, B. 1997 Genetic differentiation among natural populations of the Nile tilápia Oreochromis niloticus (Teleostei Cichlidae).Heredity, 79: 88-96.
ALJANABI, S.M. e MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.Nucleic Acids Research, 25: 4692-4693.
APPLEYARD, S.A. e MATHER, P.B. 2000 Investigation into the made of inheritance of
allozyme and random amplified polymorphic DNA markers in tilápia Oreochromis
mossambicus. Aquaculture Research, 31: 435-445.
APPLEYARD, S.A.; RENWICK, J. M.; MATHER, P.B. 2001 Individual heterozygosity levels and relative growth performance in Oreochromis niloticus (L.) cultured under Fijian conditions. Aquaculture Research, 32: 287-296.
BARDAKCI, F. e SKIBINSKI, D.O.F. 1994 Application of the RAPD technique in tilápia
fish: species and subspecies identication. Heredity, 73: 117-123.
BEATTIE, J.H.; PERDUE, J.; HERSHBERGER, W.; CHEW, K. 1987 Effects of inbreeding on growth in the Pacific oyster (Crasostrea gigas). Journal of Shellfish Research, 6: 25-28.
BEAUMONT, A.R. e BUDD, M.D. 1983 Effects of self-fertilization and other factors on the early development of the scallop Pecten maximus.Marine Biology, 76: 285-289.
BENTSEN, H.B.; EKNATH, A.E.; VERA, M.S.P.;DANTING, J.C.; BOLIVAR, H.L.; REYES, R.A.;
DIONISIO, E.E.; LONGALONG, F.M.; CIRCA,A.V.; TAYAMEN, M.M.; GJERD, B. 1988 Genetic improvement of farmed tilápias: growth performance in a complete diallel cross experiment with eight strains of Oreochromis niloticus. Aquaculture, 160: 145-173.
BHASSU, S.; YUSOFF, K.; PANANDAM, M.;EMBONG, W.K.; OYYAN, S.; TAN, S.G. 2004
The genetic structure of Oreochromis spp. (tilapia) populations in Malaysia as revealed by microsatellite DNA analysis. Biochemical Genetics, 42: 217-229.
CARLETON, K.L.; STREELMAN, J.T.; LEE, B.Y.; GARNHART, N.; KIDD, M.; KOCHER, T.D.
2002 Rapid isolation of CA microsatellites from the tilapia genome International Society for Animal Genetics. Animal Genetic, 33: 140-144.
DAN, N.C. e LITTLE, D.C. 2000 The culture performance of monosex and mixed-sex
newseason and overwintered fry in three strains of Nile tilápia (Oreochromis niloticus) in northern Vietnam. Aquaculture, 184: 221-231.
DEY, M.; EKNATH, A.E.; SIFA, L.; HUSSAIN, M.G.; THIEN, T.M.; HAO, N.V.; AYPA, S.;
PONGTHANA, N. 2000 Performance and nature of genetically improved farmed tilápia: a bioeconomic analysis. Aquaculture Economics and Management, 4: 85-108.
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KOMEN, H. 2009 Effects of relatedness and inbreeding on reproductive success of Nile tilapia (Oreochromis niloticus). Aquaculture, 294: 180-186.
GJEDREM, T. 1997 Selective breeding to improve aquaculture production. World Aquaculture, 22:33-45.
GOUDET, T. 1995 FSTAT (version 1.2): a computer program to calculate F- statistic. Journal of Heredity, 86: 485-486.
HASSANIEN, H.A. e GILBEY, J. 2005 Genetic diversity and differentiation of Nile tilápia
(Oreochromis niloticus) revealed by DNA microsatellites. Aquaculture Research, 36: 1450í 1457.
HASSANIEN H.A.; ELNADY M.; OBEIDA A.;ITRIBY H. 2004 Genetic diversity of Nile tilapia populations revealed by randomly amplified polymorphic DNA (RAPD). Aquaculture Research, 35: 587-593.
KEYS S.J.; CROCOS P.; BURRIDGE, C.Y.; COMAN, G.; DAVIS, G.; PRESTON, N. 2004 Comparative growth and survival of inbred and outbred Penaeus (Marsupenaeus) japonicus, reared under controlled environment conditions: indications of inbreeding depression. Aquaculture, 241:151-168.
KIRPICHNIKOV, V.S. 1992 Adaptative nature of intrapopulational biochemical polymorphism in fish. Journal of Fish Biology, 40:1-16.
KOCHER, T.D.; LEE, W.J.; SOBELEWSKA, H.;PENMAM, D.; McANDREW, B.J. 1998 A genetic linkage map of cichlid fish, the tilápia (Oreochromis noloticus). Genetic, 148: 1225-1232.
LANNAN, J. 1980 Broodstock management os Crassostrea gigas. IV. Inbreeding and larval survival. Aquaculture, 21: 353-356.
MACARANAS, J.M.; AGUSTIN, L.Q.; ABLAN, M.C.A.; PANTE, M.J.R.; EKNATH, A.E.;
PULLIN R.S.V. 1995 Genetic improvement of farmed tilapias: biochemical characterization of strain deference in Nile tilapia. Aquaculture International, 3: 43-54.
MALLET, A.L. e HALEY, L.E. 1983 Effects of inbreeding on larval and spat performance in the American oyster. Aquaculture, 33: 29-235.
MELO, D.C.; OLIVERA, D.A.; RIBEIRO, L.P.; TEIXEIRA, C.S.; SOUZA, A.B.; COELHO,
E.G.A.; CREPALDI, D.V.; TEIXEIRA, E.A. 2006 Caracterização genética de seis plantéis
comerciais de tilápia (Oreochromis sp.) utilizando marcadores microssatélites. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58: 87-93.
MOREIRA, A. A.; HILSDORF, A.N.; SILVA, J.V.;SOUZA, U.R. 2007 Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites. Pesquisa Agropecuária Brasileira, 4: 521-526.
MOSS, S.M. e ARCE, S.M. 2007 Effects of inbreeding on survival and growth of Pacific white shrimp Penaeus (Litopenaeus) vannamei. Aquaculture, 272:30-37.
NYINGI, D.; DE VOS, L.; AMAN, R.; AGNíˆSE, J.2009 Genetic characterization of an unknown and endangered native population of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758) (Cichlidae; Teleostei) in the Loboi Swamp (Kenya). Aquaculture, 297: 57í 63.
POVH, J.A.; MOREIRA, H.L.M.; RIBEIRO, R.P.; PRIOLI, A.J. ; BLANCK, D.V.; GASPARINO, E.;STREIT, D.P. 2005 Estimativa da variabilidade genética em linhagens de tilápia do Nilo (Oreochromis niloticus) com a técnica de RAPD. Acta Scientiarum Animal Sciences, 27: 1-10.
RAYMOND, M. e ROUSSET, F. 1995 Genepop:population genetics software for exact tests and ecumenism, Ver. 3.4. Jounal of Heredity, 86: 248-249.
RIDHA, M. 2006 Comparative study of growth performance of three strains of Nile tilápia, Oreochromis niloticus, L. at two stocking densities. Aquaculture Research, 37: 172-179.
RESENDE, E.K.; LEGAT, A.P.; OLIVEIRA, C.A.L.; RIBEIRO, R.P. 2010 Melhoramento
Animal no Brasil: uma visão crítica. Espécies Aquáticas. In: SIMPÓSIO BRASILEIRO DE
MELHORAMENTO ANIMAL, 8., Maringá, 2010.Anais... Sociedade Brasileira de melhoramentoAnimal. v.1., p.1-20.
ROGNON, X. e GUYOMARD R. 1997 Mitochondrial DNA differentiation among East and West African Nile tilapia populations. Journal of Fish Biology, 50: 204-207.
ROMANA-EGUIA, M.R.R.; IKEDAB, M.; BASIAOA,Z.U.; TANIGUCHIB, N. 2004 Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture, 236: 131í 150.
ROMANA-EGUIA, M.R.R.; IKEDA M.; BASIAO, Z.;TANIGUCHI, N. 2005 Genetic changes during mass selection for growth in Nile tilapia, Oreochromis niloticus (L.), assessed by microsatellites. Aquaculture Research, 36: 69-78.
ROUSSET, F. e RAYMOND, M. 1995 Testing heterozygote excess and deficiency. Genetics,140: 1413-1419.
RUTTEN, M.J.M.; KOMEN, H.; DEEREMBERG,R.M.; SIWEK M.; BOVENHUIS, H. 2004 Genetic characterization of four strains of nile tilapia (Oreochromis niloticus L.) using microsatellite markers. Animal Genetics, 35: 93-97.
RYE, M. e MAO, I.L. 1998 Nonadditive genetic effects and inbreeding depression for body weight in Atlantic salmon (Salmo salar L.). Livestock Production Science, 57: 15-22.
SU, G.S.; LIELJEDAHL, L.; GALL, G.A. 1996 Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss).Aquaculture, 142: 139-142.
TACHIBANA, L.; CASTAGNOLLI, N.; PEZZATO,L.E.; BARROS, M.M.; VALLE, J.B.; SIQUEIRA, M.R. 2004 Desempenho de diferentes linhagens de tilápia do Nilo (Oreochromis niloticus) na fase de reversão sexual. Acta Scientiarum Animal Sciences, 26(3): 305-311.
TAYAMEN, M.M.; REYES, R.A.; DANTING, M.J.;MENDOZA, A.M.; MARQUEZ, E.B.; SALGUET,A.C.; GONZALES, R.C.; ABELLA, T.A.; VERACRUZ, E.M. 2002 Tilapia broodstock development for saline waters in the Philippines. Naga-Iclarm Quarterly, 25: 32í 36.
WEIR, B.S. 1990 Genetic data analysis: methods of discrete population genetic data. Suderland,Massachusetts: Sinauer Associates. 337p.
WEIR, B.S. e COCKERHAM, C.C. 1984 Estimating Fstatistics for the analysis of population structure.Evolution, 38: 1358-1370.
WRIGHT, S. 1922 Coefficient of inbreeding and relationship. American Naturalist, 56: 330-338.
WRIGHT, S. 1978 Evolution and genetic population.Variability within and among natural populations.Chicago III: University of Chicago Press. v.4, 590p.
ZIMMERMANN, S. 1999 Incubação artificial. Técnica permite a produção de tilápias do Nilo geneticamente superiores. Panorama da Aquicultura, 9: 5-21.
Oreochromis niloticus, Oreochromis mossambicus and their hybrids. Aquaculture, 247: 189-201.
AGNESSE, J.; ADEPO GOURENE, B.; ABBAN, E.K.; FERMOSN, B. 1997 Genetic differentiation among natural populations of the Nile tilápia Oreochromis niloticus (Teleostei Cichlidae).Heredity, 79: 88-96.
ALJANABI, S.M. e MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.Nucleic Acids Research, 25: 4692-4693.
APPLEYARD, S.A. e MATHER, P.B. 2000 Investigation into the made of inheritance of
allozyme and random amplified polymorphic DNA markers in tilápia Oreochromis
mossambicus. Aquaculture Research, 31: 435-445.
APPLEYARD, S.A.; RENWICK, J. M.; MATHER, P.B. 2001 Individual heterozygosity levels and relative growth performance in Oreochromis niloticus (L.) cultured under Fijian conditions. Aquaculture Research, 32: 287-296.
BARDAKCI, F. e SKIBINSKI, D.O.F. 1994 Application of the RAPD technique in tilápia
fish: species and subspecies identication. Heredity, 73: 117-123.
BEATTIE, J.H.; PERDUE, J.; HERSHBERGER, W.; CHEW, K. 1987 Effects of inbreeding on growth in the Pacific oyster (Crasostrea gigas). Journal of Shellfish Research, 6: 25-28.
BEAUMONT, A.R. e BUDD, M.D. 1983 Effects of self-fertilization and other factors on the early development of the scallop Pecten maximus.Marine Biology, 76: 285-289.
BENTSEN, H.B.; EKNATH, A.E.; VERA, M.S.P.;DANTING, J.C.; BOLIVAR, H.L.; REYES, R.A.;
DIONISIO, E.E.; LONGALONG, F.M.; CIRCA,A.V.; TAYAMEN, M.M.; GJERD, B. 1988 Genetic improvement of farmed tilápias: growth performance in a complete diallel cross experiment with eight strains of Oreochromis niloticus. Aquaculture, 160: 145-173.
BHASSU, S.; YUSOFF, K.; PANANDAM, M.;EMBONG, W.K.; OYYAN, S.; TAN, S.G. 2004
The genetic structure of Oreochromis spp. (tilapia) populations in Malaysia as revealed by microsatellite DNA analysis. Biochemical Genetics, 42: 217-229.
CARLETON, K.L.; STREELMAN, J.T.; LEE, B.Y.; GARNHART, N.; KIDD, M.; KOCHER, T.D.
2002 Rapid isolation of CA microsatellites from the tilapia genome International Society for Animal Genetics. Animal Genetic, 33: 140-144.
DAN, N.C. e LITTLE, D.C. 2000 The culture performance of monosex and mixed-sex
newseason and overwintered fry in three strains of Nile tilápia (Oreochromis niloticus) in northern Vietnam. Aquaculture, 184: 221-231.
DEY, M.; EKNATH, A.E.; SIFA, L.; HUSSAIN, M.G.; THIEN, T.M.; HAO, N.V.; AYPA, S.;
PONGTHANA, N. 2000 Performance and nature of genetically improved farmed tilápia: a bioeconomic analysis. Aquaculture Economics and Management, 4: 85-108.
ESPÍNDOLA, M. 2007 Caracterização genética de reprodutores de tilápia: estratégias para a manutenção da variabilidade. Recife. 77p. (Tese de doutorado. Universidade Federal Rural de Pernambuco). Disponível em: http://www.pgpa.ufrpe.br/Trabalhos/2007/T2007mes.pdf
FALCONER, D.S. e MACKAY, T.F.C. 1996 Introduction to quantitative genetics, 4ª ed.
Edinburgh: Longman Group Limited. 464p.
FESSEHAYE, Y.; BOUVENHUIS, H.; REZK, M.A.; CROOIJMANS, R.; VAN ARENDONK, J.;
KOMEN, H. 2009 Effects of relatedness and inbreeding on reproductive success of Nile tilapia (Oreochromis niloticus). Aquaculture, 294: 180-186.
GJEDREM, T. 1997 Selective breeding to improve aquaculture production. World Aquaculture, 22:33-45.
GOUDET, T. 1995 FSTAT (version 1.2): a computer program to calculate F- statistic. Journal of Heredity, 86: 485-486.
HASSANIEN, H.A. e GILBEY, J. 2005 Genetic diversity and differentiation of Nile tilápia
(Oreochromis niloticus) revealed by DNA microsatellites. Aquaculture Research, 36: 1450í 1457.
HASSANIEN H.A.; ELNADY M.; OBEIDA A.;ITRIBY H. 2004 Genetic diversity of Nile tilapia populations revealed by randomly amplified polymorphic DNA (RAPD). Aquaculture Research, 35: 587-593.
KEYS S.J.; CROCOS P.; BURRIDGE, C.Y.; COMAN, G.; DAVIS, G.; PRESTON, N. 2004 Comparative growth and survival of inbred and outbred Penaeus (Marsupenaeus) japonicus, reared under controlled environment conditions: indications of inbreeding depression. Aquaculture, 241:151-168.
KIRPICHNIKOV, V.S. 1992 Adaptative nature of intrapopulational biochemical polymorphism in fish. Journal of Fish Biology, 40:1-16.
KOCHER, T.D.; LEE, W.J.; SOBELEWSKA, H.;PENMAM, D.; McANDREW, B.J. 1998 A genetic linkage map of cichlid fish, the tilápia (Oreochromis noloticus). Genetic, 148: 1225-1232.
LANNAN, J. 1980 Broodstock management os Crassostrea gigas. IV. Inbreeding and larval survival. Aquaculture, 21: 353-356.
MACARANAS, J.M.; AGUSTIN, L.Q.; ABLAN, M.C.A.; PANTE, M.J.R.; EKNATH, A.E.;
PULLIN R.S.V. 1995 Genetic improvement of farmed tilapias: biochemical characterization of strain deference in Nile tilapia. Aquaculture International, 3: 43-54.
MALLET, A.L. e HALEY, L.E. 1983 Effects of inbreeding on larval and spat performance in the American oyster. Aquaculture, 33: 29-235.
MELO, D.C.; OLIVERA, D.A.; RIBEIRO, L.P.; TEIXEIRA, C.S.; SOUZA, A.B.; COELHO,
E.G.A.; CREPALDI, D.V.; TEIXEIRA, E.A. 2006 Caracterização genética de seis plantéis
comerciais de tilápia (Oreochromis sp.) utilizando marcadores microssatélites. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58: 87-93.
MOREIRA, A. A.; HILSDORF, A.N.; SILVA, J.V.;SOUZA, U.R. 2007 Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites. Pesquisa Agropecuária Brasileira, 4: 521-526.
MOSS, S.M. e ARCE, S.M. 2007 Effects of inbreeding on survival and growth of Pacific white shrimp Penaeus (Litopenaeus) vannamei. Aquaculture, 272:30-37.
NYINGI, D.; DE VOS, L.; AMAN, R.; AGNíˆSE, J.2009 Genetic characterization of an unknown and endangered native population of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758) (Cichlidae; Teleostei) in the Loboi Swamp (Kenya). Aquaculture, 297: 57í 63.
POVH, J.A.; MOREIRA, H.L.M.; RIBEIRO, R.P.; PRIOLI, A.J. ; BLANCK, D.V.; GASPARINO, E.;STREIT, D.P. 2005 Estimativa da variabilidade genética em linhagens de tilápia do Nilo (Oreochromis niloticus) com a técnica de RAPD. Acta Scientiarum Animal Sciences, 27: 1-10.
RAYMOND, M. e ROUSSET, F. 1995 Genepop:population genetics software for exact tests and ecumenism, Ver. 3.4. Jounal of Heredity, 86: 248-249.
RIDHA, M. 2006 Comparative study of growth performance of three strains of Nile tilápia, Oreochromis niloticus, L. at two stocking densities. Aquaculture Research, 37: 172-179.
RESENDE, E.K.; LEGAT, A.P.; OLIVEIRA, C.A.L.; RIBEIRO, R.P. 2010 Melhoramento
Animal no Brasil: uma visão crítica. Espécies Aquáticas. In: SIMPÓSIO BRASILEIRO DE
MELHORAMENTO ANIMAL, 8., Maringá, 2010.Anais... Sociedade Brasileira de melhoramentoAnimal. v.1., p.1-20.
ROGNON, X. e GUYOMARD R. 1997 Mitochondrial DNA differentiation among East and West African Nile tilapia populations. Journal of Fish Biology, 50: 204-207.
ROMANA-EGUIA, M.R.R.; IKEDAB, M.; BASIAOA,Z.U.; TANIGUCHIB, N. 2004 Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture, 236: 131í 150.
ROMANA-EGUIA, M.R.R.; IKEDA M.; BASIAO, Z.;TANIGUCHI, N. 2005 Genetic changes during mass selection for growth in Nile tilapia, Oreochromis niloticus (L.), assessed by microsatellites. Aquaculture Research, 36: 69-78.
ROUSSET, F. e RAYMOND, M. 1995 Testing heterozygote excess and deficiency. Genetics,140: 1413-1419.
RUTTEN, M.J.M.; KOMEN, H.; DEEREMBERG,R.M.; SIWEK M.; BOVENHUIS, H. 2004 Genetic characterization of four strains of nile tilapia (Oreochromis niloticus L.) using microsatellite markers. Animal Genetics, 35: 93-97.
RYE, M. e MAO, I.L. 1998 Nonadditive genetic effects and inbreeding depression for body weight in Atlantic salmon (Salmo salar L.). Livestock Production Science, 57: 15-22.
SU, G.S.; LIELJEDAHL, L.; GALL, G.A. 1996 Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss).Aquaculture, 142: 139-142.
TACHIBANA, L.; CASTAGNOLLI, N.; PEZZATO,L.E.; BARROS, M.M.; VALLE, J.B.; SIQUEIRA, M.R. 2004 Desempenho de diferentes linhagens de tilápia do Nilo (Oreochromis niloticus) na fase de reversão sexual. Acta Scientiarum Animal Sciences, 26(3): 305-311.
TAYAMEN, M.M.; REYES, R.A.; DANTING, M.J.;MENDOZA, A.M.; MARQUEZ, E.B.; SALGUET,A.C.; GONZALES, R.C.; ABELLA, T.A.; VERACRUZ, E.M. 2002 Tilapia broodstock development for saline waters in the Philippines. Naga-Iclarm Quarterly, 25: 32í 36.
WEIR, B.S. 1990 Genetic data analysis: methods of discrete population genetic data. Suderland,Massachusetts: Sinauer Associates. 337p.
WEIR, B.S. e COCKERHAM, C.C. 1984 Estimating Fstatistics for the analysis of population structure.Evolution, 38: 1358-1370.
WRIGHT, S. 1922 Coefficient of inbreeding and relationship. American Naturalist, 56: 330-338.
WRIGHT, S. 1978 Evolution and genetic population.Variability within and among natural populations.Chicago III: University of Chicago Press. v.4, 590p.
ZIMMERMANN, S. 1999 Incubação artificial. Técnica permite a produção de tilápias do Nilo geneticamente superiores. Panorama da Aquicultura, 9: 5-21.
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2018-11-11
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