Análise da diversidade genética de tilápias cultivadas no estado de Santa Catarina (Brasil) utilizando marcadores microssatélites
Palavras-chave:
piscicultura, melhoramento genético, marcadores molecularesResumo
Três variedades de tilápias cultivadas em Santa Catarina pertencentes í estação de piscicultura Panamá-Unisul foram selecionadas para a avaliação da diversidade genética por meio da análise de marcadores microssatélites. Os testes de diferenciação genética e genotípica, assim como o grau de divergência genética entre as populações (FST), foram estatisticamente significativos (P<0,01). Quando os valores de FST foram obtidos entre cada par de populações, observou-se maior divergência genética entre a variedade GIFT e as populações VERMELHA (VERM) (FST = 0,11) e PANAMÁ (PAN) (FST = 0,18). A variedade GIFT (FIS = 0,16) apresentou o menor coeficiente de endogamia quando comparada com as variedades locais PAN (FIS = 0,33) e VERM (FIS = 0,28), refletindo seu histórico de controle de pedigree. Tendo em vista os resultados obtidos, é possível perceber a importí¢ncia do controle no manejo dos plantéis para manter a diversidade genética das populações.
Referências
ABU HENA, M.K. e MAIR, G.C. 2005 Salinity tolerance in superior genotypes of tilapia,
Oreochromis niloticus, Oreochromis mossambicus and their hybrids. Aquaculture, 247: 189-201.
AGNESSE, J.; ADEPO GOURENE, B.; ABBAN, E.K.; FERMOSN, B. 1997 Genetic differentiation among natural populations of the Nile tilápia Oreochromis niloticus (Teleostei Cichlidae).Heredity, 79: 88-96.
ALJANABI, S.M. e MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.Nucleic Acids Research, 25: 4692-4693.
APPLEYARD, S.A. e MATHER, P.B. 2000 Investigation into the made of inheritance of
allozyme and random amplified polymorphic DNA markers in tilápia Oreochromis
mossambicus. Aquaculture Research, 31: 435-445.
APPLEYARD, S.A.; RENWICK, J. M.; MATHER, P.B. 2001 Individual heterozygosity levels and relative growth performance in Oreochromis niloticus (L.) cultured under Fijian conditions. Aquaculture Research, 32: 287-296.
BARDAKCI, F. e SKIBINSKI, D.O.F. 1994 Application of the RAPD technique in tilápia
fish: species and subspecies identication. Heredity, 73: 117-123.
BEATTIE, J.H.; PERDUE, J.; HERSHBERGER, W.; CHEW, K. 1987 Effects of inbreeding on growth in the Pacific oyster (Crasostrea gigas). Journal of Shellfish Research, 6: 25-28.
BEAUMONT, A.R. e BUDD, M.D. 1983 Effects of self-fertilization and other factors on the early development of the scallop Pecten maximus.Marine Biology, 76: 285-289.
BENTSEN, H.B.; EKNATH, A.E.; VERA, M.S.P.;DANTING, J.C.; BOLIVAR, H.L.; REYES, R.A.;
DIONISIO, E.E.; LONGALONG, F.M.; CIRCA,A.V.; TAYAMEN, M.M.; GJERD, B. 1988 Genetic improvement of farmed tilápias: growth performance in a complete diallel cross experiment with eight strains of Oreochromis niloticus. Aquaculture, 160: 145-173.
BHASSU, S.; YUSOFF, K.; PANANDAM, M.;EMBONG, W.K.; OYYAN, S.; TAN, S.G. 2004
The genetic structure of Oreochromis spp. (tilapia) populations in Malaysia as revealed by microsatellite DNA analysis. Biochemical Genetics, 42: 217-229.
CARLETON, K.L.; STREELMAN, J.T.; LEE, B.Y.; GARNHART, N.; KIDD, M.; KOCHER, T.D.
2002 Rapid isolation of CA microsatellites from the tilapia genome International Society for Animal Genetics. Animal Genetic, 33: 140-144.
DAN, N.C. e LITTLE, D.C. 2000 The culture performance of monosex and mixed-sex
newseason and overwintered fry in three strains of Nile tilápia (Oreochromis niloticus) in northern Vietnam. Aquaculture, 184: 221-231.
DEY, M.; EKNATH, A.E.; SIFA, L.; HUSSAIN, M.G.; THIEN, T.M.; HAO, N.V.; AYPA, S.;
PONGTHANA, N. 2000 Performance and nature of genetically improved farmed tilápia: a bioeconomic analysis. Aquaculture Economics and Management, 4: 85-108.
ESPÍNDOLA, M. 2007 Caracterização genética de reprodutores de tilápia: estratégias para a manutenção da variabilidade. Recife. 77p. (Tese de doutorado. Universidade Federal Rural de Pernambuco). Disponível em: http://www.pgpa.ufrpe.br/Trabalhos/2007/T2007mes.pdf
FALCONER, D.S. e MACKAY, T.F.C. 1996 Introduction to quantitative genetics, 4ª ed.
Edinburgh: Longman Group Limited. 464p.
FESSEHAYE, Y.; BOUVENHUIS, H.; REZK, M.A.; CROOIJMANS, R.; VAN ARENDONK, J.;
KOMEN, H. 2009 Effects of relatedness and inbreeding on reproductive success of Nile tilapia (Oreochromis niloticus). Aquaculture, 294: 180-186.
GJEDREM, T. 1997 Selective breeding to improve aquaculture production. World Aquaculture, 22:33-45.
GOUDET, T. 1995 FSTAT (version 1.2): a computer program to calculate F- statistic. Journal of Heredity, 86: 485-486.
HASSANIEN, H.A. e GILBEY, J. 2005 Genetic diversity and differentiation of Nile tilápia
(Oreochromis niloticus) revealed by DNA microsatellites. Aquaculture Research, 36: 1450í 1457.
HASSANIEN H.A.; ELNADY M.; OBEIDA A.;ITRIBY H. 2004 Genetic diversity of Nile tilapia populations revealed by randomly amplified polymorphic DNA (RAPD). Aquaculture Research, 35: 587-593.
KEYS S.J.; CROCOS P.; BURRIDGE, C.Y.; COMAN, G.; DAVIS, G.; PRESTON, N. 2004 Comparative growth and survival of inbred and outbred Penaeus (Marsupenaeus) japonicus, reared under controlled environment conditions: indications of inbreeding depression. Aquaculture, 241:151-168.
KIRPICHNIKOV, V.S. 1992 Adaptative nature of intrapopulational biochemical polymorphism in fish. Journal of Fish Biology, 40:1-16.
KOCHER, T.D.; LEE, W.J.; SOBELEWSKA, H.;PENMAM, D.; McANDREW, B.J. 1998 A genetic linkage map of cichlid fish, the tilápia (Oreochromis noloticus). Genetic, 148: 1225-1232.
LANNAN, J. 1980 Broodstock management os Crassostrea gigas. IV. Inbreeding and larval survival. Aquaculture, 21: 353-356.
MACARANAS, J.M.; AGUSTIN, L.Q.; ABLAN, M.C.A.; PANTE, M.J.R.; EKNATH, A.E.;
PULLIN R.S.V. 1995 Genetic improvement of farmed tilapias: biochemical characterization of strain deference in Nile tilapia. Aquaculture International, 3: 43-54.
MALLET, A.L. e HALEY, L.E. 1983 Effects of inbreeding on larval and spat performance in the American oyster. Aquaculture, 33: 29-235.
MELO, D.C.; OLIVERA, D.A.; RIBEIRO, L.P.; TEIXEIRA, C.S.; SOUZA, A.B.; COELHO,
E.G.A.; CREPALDI, D.V.; TEIXEIRA, E.A. 2006 Caracterização genética de seis plantéis
comerciais de tilápia (Oreochromis sp.) utilizando marcadores microssatélites. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58: 87-93.
MOREIRA, A. A.; HILSDORF, A.N.; SILVA, J.V.;SOUZA, U.R. 2007 Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites. Pesquisa Agropecuária Brasileira, 4: 521-526.
MOSS, S.M. e ARCE, S.M. 2007 Effects of inbreeding on survival and growth of Pacific white shrimp Penaeus (Litopenaeus) vannamei. Aquaculture, 272:30-37.
NYINGI, D.; DE VOS, L.; AMAN, R.; AGNíˆSE, J.2009 Genetic characterization of an unknown and endangered native population of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758) (Cichlidae; Teleostei) in the Loboi Swamp (Kenya). Aquaculture, 297: 57í 63.
POVH, J.A.; MOREIRA, H.L.M.; RIBEIRO, R.P.; PRIOLI, A.J. ; BLANCK, D.V.; GASPARINO, E.;STREIT, D.P. 2005 Estimativa da variabilidade genética em linhagens de tilápia do Nilo (Oreochromis niloticus) com a técnica de RAPD. Acta Scientiarum Animal Sciences, 27: 1-10.
RAYMOND, M. e ROUSSET, F. 1995 Genepop:population genetics software for exact tests and ecumenism, Ver. 3.4. Jounal of Heredity, 86: 248-249.
RIDHA, M. 2006 Comparative study of growth performance of three strains of Nile tilápia, Oreochromis niloticus, L. at two stocking densities. Aquaculture Research, 37: 172-179.
RESENDE, E.K.; LEGAT, A.P.; OLIVEIRA, C.A.L.; RIBEIRO, R.P. 2010 Melhoramento
Animal no Brasil: uma visão crítica. Espécies Aquáticas. In: SIMPÓSIO BRASILEIRO DE
MELHORAMENTO ANIMAL, 8., Maringá, 2010.Anais... Sociedade Brasileira de melhoramentoAnimal. v.1., p.1-20.
ROGNON, X. e GUYOMARD R. 1997 Mitochondrial DNA differentiation among East and West African Nile tilapia populations. Journal of Fish Biology, 50: 204-207.
ROMANA-EGUIA, M.R.R.; IKEDAB, M.; BASIAOA,Z.U.; TANIGUCHIB, N. 2004 Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture, 236: 131í 150.
ROMANA-EGUIA, M.R.R.; IKEDA M.; BASIAO, Z.;TANIGUCHI, N. 2005 Genetic changes during mass selection for growth in Nile tilapia, Oreochromis niloticus (L.), assessed by microsatellites. Aquaculture Research, 36: 69-78.
ROUSSET, F. e RAYMOND, M. 1995 Testing heterozygote excess and deficiency. Genetics,140: 1413-1419.
RUTTEN, M.J.M.; KOMEN, H.; DEEREMBERG,R.M.; SIWEK M.; BOVENHUIS, H. 2004 Genetic characterization of four strains of nile tilapia (Oreochromis niloticus L.) using microsatellite markers. Animal Genetics, 35: 93-97.
RYE, M. e MAO, I.L. 1998 Nonadditive genetic effects and inbreeding depression for body weight in Atlantic salmon (Salmo salar L.). Livestock Production Science, 57: 15-22.
SU, G.S.; LIELJEDAHL, L.; GALL, G.A. 1996 Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss).Aquaculture, 142: 139-142.
TACHIBANA, L.; CASTAGNOLLI, N.; PEZZATO,L.E.; BARROS, M.M.; VALLE, J.B.; SIQUEIRA, M.R. 2004 Desempenho de diferentes linhagens de tilápia do Nilo (Oreochromis niloticus) na fase de reversão sexual. Acta Scientiarum Animal Sciences, 26(3): 305-311.
TAYAMEN, M.M.; REYES, R.A.; DANTING, M.J.;MENDOZA, A.M.; MARQUEZ, E.B.; SALGUET,A.C.; GONZALES, R.C.; ABELLA, T.A.; VERACRUZ, E.M. 2002 Tilapia broodstock development for saline waters in the Philippines. Naga-Iclarm Quarterly, 25: 32í 36.
WEIR, B.S. 1990 Genetic data analysis: methods of discrete population genetic data. Suderland,Massachusetts: Sinauer Associates. 337p.
WEIR, B.S. e COCKERHAM, C.C. 1984 Estimating Fstatistics for the analysis of population structure.Evolution, 38: 1358-1370.
WRIGHT, S. 1922 Coefficient of inbreeding and relationship. American Naturalist, 56: 330-338.
WRIGHT, S. 1978 Evolution and genetic population.Variability within and among natural populations.Chicago III: University of Chicago Press. v.4, 590p.
ZIMMERMANN, S. 1999 Incubação artificial. Técnica permite a produção de tilápias do Nilo geneticamente superiores. Panorama da Aquicultura, 9: 5-21.
Oreochromis niloticus, Oreochromis mossambicus and their hybrids. Aquaculture, 247: 189-201.
AGNESSE, J.; ADEPO GOURENE, B.; ABBAN, E.K.; FERMOSN, B. 1997 Genetic differentiation among natural populations of the Nile tilápia Oreochromis niloticus (Teleostei Cichlidae).Heredity, 79: 88-96.
ALJANABI, S.M. e MARTINEZ, I. 1997 Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.Nucleic Acids Research, 25: 4692-4693.
APPLEYARD, S.A. e MATHER, P.B. 2000 Investigation into the made of inheritance of
allozyme and random amplified polymorphic DNA markers in tilápia Oreochromis
mossambicus. Aquaculture Research, 31: 435-445.
APPLEYARD, S.A.; RENWICK, J. M.; MATHER, P.B. 2001 Individual heterozygosity levels and relative growth performance in Oreochromis niloticus (L.) cultured under Fijian conditions. Aquaculture Research, 32: 287-296.
BARDAKCI, F. e SKIBINSKI, D.O.F. 1994 Application of the RAPD technique in tilápia
fish: species and subspecies identication. Heredity, 73: 117-123.
BEATTIE, J.H.; PERDUE, J.; HERSHBERGER, W.; CHEW, K. 1987 Effects of inbreeding on growth in the Pacific oyster (Crasostrea gigas). Journal of Shellfish Research, 6: 25-28.
BEAUMONT, A.R. e BUDD, M.D. 1983 Effects of self-fertilization and other factors on the early development of the scallop Pecten maximus.Marine Biology, 76: 285-289.
BENTSEN, H.B.; EKNATH, A.E.; VERA, M.S.P.;DANTING, J.C.; BOLIVAR, H.L.; REYES, R.A.;
DIONISIO, E.E.; LONGALONG, F.M.; CIRCA,A.V.; TAYAMEN, M.M.; GJERD, B. 1988 Genetic improvement of farmed tilápias: growth performance in a complete diallel cross experiment with eight strains of Oreochromis niloticus. Aquaculture, 160: 145-173.
BHASSU, S.; YUSOFF, K.; PANANDAM, M.;EMBONG, W.K.; OYYAN, S.; TAN, S.G. 2004
The genetic structure of Oreochromis spp. (tilapia) populations in Malaysia as revealed by microsatellite DNA analysis. Biochemical Genetics, 42: 217-229.
CARLETON, K.L.; STREELMAN, J.T.; LEE, B.Y.; GARNHART, N.; KIDD, M.; KOCHER, T.D.
2002 Rapid isolation of CA microsatellites from the tilapia genome International Society for Animal Genetics. Animal Genetic, 33: 140-144.
DAN, N.C. e LITTLE, D.C. 2000 The culture performance of monosex and mixed-sex
newseason and overwintered fry in three strains of Nile tilápia (Oreochromis niloticus) in northern Vietnam. Aquaculture, 184: 221-231.
DEY, M.; EKNATH, A.E.; SIFA, L.; HUSSAIN, M.G.; THIEN, T.M.; HAO, N.V.; AYPA, S.;
PONGTHANA, N. 2000 Performance and nature of genetically improved farmed tilápia: a bioeconomic analysis. Aquaculture Economics and Management, 4: 85-108.
ESPÍNDOLA, M. 2007 Caracterização genética de reprodutores de tilápia: estratégias para a manutenção da variabilidade. Recife. 77p. (Tese de doutorado. Universidade Federal Rural de Pernambuco). Disponível em: http://www.pgpa.ufrpe.br/Trabalhos/2007/T2007mes.pdf
FALCONER, D.S. e MACKAY, T.F.C. 1996 Introduction to quantitative genetics, 4ª ed.
Edinburgh: Longman Group Limited. 464p.
FESSEHAYE, Y.; BOUVENHUIS, H.; REZK, M.A.; CROOIJMANS, R.; VAN ARENDONK, J.;
KOMEN, H. 2009 Effects of relatedness and inbreeding on reproductive success of Nile tilapia (Oreochromis niloticus). Aquaculture, 294: 180-186.
GJEDREM, T. 1997 Selective breeding to improve aquaculture production. World Aquaculture, 22:33-45.
GOUDET, T. 1995 FSTAT (version 1.2): a computer program to calculate F- statistic. Journal of Heredity, 86: 485-486.
HASSANIEN, H.A. e GILBEY, J. 2005 Genetic diversity and differentiation of Nile tilápia
(Oreochromis niloticus) revealed by DNA microsatellites. Aquaculture Research, 36: 1450í 1457.
HASSANIEN H.A.; ELNADY M.; OBEIDA A.;ITRIBY H. 2004 Genetic diversity of Nile tilapia populations revealed by randomly amplified polymorphic DNA (RAPD). Aquaculture Research, 35: 587-593.
KEYS S.J.; CROCOS P.; BURRIDGE, C.Y.; COMAN, G.; DAVIS, G.; PRESTON, N. 2004 Comparative growth and survival of inbred and outbred Penaeus (Marsupenaeus) japonicus, reared under controlled environment conditions: indications of inbreeding depression. Aquaculture, 241:151-168.
KIRPICHNIKOV, V.S. 1992 Adaptative nature of intrapopulational biochemical polymorphism in fish. Journal of Fish Biology, 40:1-16.
KOCHER, T.D.; LEE, W.J.; SOBELEWSKA, H.;PENMAM, D.; McANDREW, B.J. 1998 A genetic linkage map of cichlid fish, the tilápia (Oreochromis noloticus). Genetic, 148: 1225-1232.
LANNAN, J. 1980 Broodstock management os Crassostrea gigas. IV. Inbreeding and larval survival. Aquaculture, 21: 353-356.
MACARANAS, J.M.; AGUSTIN, L.Q.; ABLAN, M.C.A.; PANTE, M.J.R.; EKNATH, A.E.;
PULLIN R.S.V. 1995 Genetic improvement of farmed tilapias: biochemical characterization of strain deference in Nile tilapia. Aquaculture International, 3: 43-54.
MALLET, A.L. e HALEY, L.E. 1983 Effects of inbreeding on larval and spat performance in the American oyster. Aquaculture, 33: 29-235.
MELO, D.C.; OLIVERA, D.A.; RIBEIRO, L.P.; TEIXEIRA, C.S.; SOUZA, A.B.; COELHO,
E.G.A.; CREPALDI, D.V.; TEIXEIRA, E.A. 2006 Caracterização genética de seis plantéis
comerciais de tilápia (Oreochromis sp.) utilizando marcadores microssatélites. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58: 87-93.
MOREIRA, A. A.; HILSDORF, A.N.; SILVA, J.V.;SOUZA, U.R. 2007 Variabilidade genética de duas variedades de tilápia nilótica por meio de marcadores microssatélites. Pesquisa Agropecuária Brasileira, 4: 521-526.
MOSS, S.M. e ARCE, S.M. 2007 Effects of inbreeding on survival and growth of Pacific white shrimp Penaeus (Litopenaeus) vannamei. Aquaculture, 272:30-37.
NYINGI, D.; DE VOS, L.; AMAN, R.; AGNíˆSE, J.2009 Genetic characterization of an unknown and endangered native population of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758) (Cichlidae; Teleostei) in the Loboi Swamp (Kenya). Aquaculture, 297: 57í 63.
POVH, J.A.; MOREIRA, H.L.M.; RIBEIRO, R.P.; PRIOLI, A.J. ; BLANCK, D.V.; GASPARINO, E.;STREIT, D.P. 2005 Estimativa da variabilidade genética em linhagens de tilápia do Nilo (Oreochromis niloticus) com a técnica de RAPD. Acta Scientiarum Animal Sciences, 27: 1-10.
RAYMOND, M. e ROUSSET, F. 1995 Genepop:population genetics software for exact tests and ecumenism, Ver. 3.4. Jounal of Heredity, 86: 248-249.
RIDHA, M. 2006 Comparative study of growth performance of three strains of Nile tilápia, Oreochromis niloticus, L. at two stocking densities. Aquaculture Research, 37: 172-179.
RESENDE, E.K.; LEGAT, A.P.; OLIVEIRA, C.A.L.; RIBEIRO, R.P. 2010 Melhoramento
Animal no Brasil: uma visão crítica. Espécies Aquáticas. In: SIMPÓSIO BRASILEIRO DE
MELHORAMENTO ANIMAL, 8., Maringá, 2010.Anais... Sociedade Brasileira de melhoramentoAnimal. v.1., p.1-20.
ROGNON, X. e GUYOMARD R. 1997 Mitochondrial DNA differentiation among East and West African Nile tilapia populations. Journal of Fish Biology, 50: 204-207.
ROMANA-EGUIA, M.R.R.; IKEDAB, M.; BASIAOA,Z.U.; TANIGUCHIB, N. 2004 Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture, 236: 131í 150.
ROMANA-EGUIA, M.R.R.; IKEDA M.; BASIAO, Z.;TANIGUCHI, N. 2005 Genetic changes during mass selection for growth in Nile tilapia, Oreochromis niloticus (L.), assessed by microsatellites. Aquaculture Research, 36: 69-78.
ROUSSET, F. e RAYMOND, M. 1995 Testing heterozygote excess and deficiency. Genetics,140: 1413-1419.
RUTTEN, M.J.M.; KOMEN, H.; DEEREMBERG,R.M.; SIWEK M.; BOVENHUIS, H. 2004 Genetic characterization of four strains of nile tilapia (Oreochromis niloticus L.) using microsatellite markers. Animal Genetics, 35: 93-97.
RYE, M. e MAO, I.L. 1998 Nonadditive genetic effects and inbreeding depression for body weight in Atlantic salmon (Salmo salar L.). Livestock Production Science, 57: 15-22.
SU, G.S.; LIELJEDAHL, L.; GALL, G.A. 1996 Effects of inbreeding on growth and reproductive traits in rainbow trout (Oncorhynchus mykiss).Aquaculture, 142: 139-142.
TACHIBANA, L.; CASTAGNOLLI, N.; PEZZATO,L.E.; BARROS, M.M.; VALLE, J.B.; SIQUEIRA, M.R. 2004 Desempenho de diferentes linhagens de tilápia do Nilo (Oreochromis niloticus) na fase de reversão sexual. Acta Scientiarum Animal Sciences, 26(3): 305-311.
TAYAMEN, M.M.; REYES, R.A.; DANTING, M.J.;MENDOZA, A.M.; MARQUEZ, E.B.; SALGUET,A.C.; GONZALES, R.C.; ABELLA, T.A.; VERACRUZ, E.M. 2002 Tilapia broodstock development for saline waters in the Philippines. Naga-Iclarm Quarterly, 25: 32í 36.
WEIR, B.S. 1990 Genetic data analysis: methods of discrete population genetic data. Suderland,Massachusetts: Sinauer Associates. 337p.
WEIR, B.S. e COCKERHAM, C.C. 1984 Estimating Fstatistics for the analysis of population structure.Evolution, 38: 1358-1370.
WRIGHT, S. 1922 Coefficient of inbreeding and relationship. American Naturalist, 56: 330-338.
WRIGHT, S. 1978 Evolution and genetic population.Variability within and among natural populations.Chicago III: University of Chicago Press. v.4, 590p.
ZIMMERMANN, S. 1999 Incubação artificial. Técnica permite a produção de tilápias do Nilo geneticamente superiores. Panorama da Aquicultura, 9: 5-21.
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2018-11-11
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