A COMPARATIVE STUDY OF THE OVARIAN HISTOLOGY OF EYESTALK ABLATED AND UNABLATED Farfantepenaeus paulensis AFTER SPAWNING
Keywords:
eyestalk ablation, gonad, histology, reproduction, Farfantepenaeus paulensisAbstract
The effect of unilateral eyestalk ablation on ovarian histology of Farfantepenaeus paulensis after spawning was evaluated in the present study. Wild females were captured in deep-sea waters of southern Brazil (27°S) and randomly divided in two groups: unilaterally eyestalk ablated and unablated (intact) females. A total of six eyestalk ablated and five unablated females were sampled after spawning in separated tanks. Morphological variables were recorded and ovaries were histologically evaluated according to oocyte type and diameter. In the ovarian tissue of unablated females, basophilic oocytes (48.8 ± 18.7 µm) were dominant (99.7 ± 0.6%), with presence significantly lower in the ablated females (93.5 ± 13.8%). Larger acidophilic oocytes (114.9 ± 16.9 µm), with yolk granules in the cytoplasm, were detected only in the ovaries of ablated females. The significantly higher occurrence of atretic oocytes (4.8 ± 10.3%) is another distinguishable feature in the ovaries of ablated females compared with the unablated ones. The presence of advanced yolky oocytes in ablated females just a few hours after spawning may indicate the effect of eyestalk ablation on the precocious rematuration of the ovary. The higher number of atretic oocytes in the ovary of ablated females could be relevant to their reproductive performance, as they represent a percentage of oocytes that were not released in the spawning process. The present results of the histology of the ovaries reveal the existence of substantial differences in the cell arrangement of ablated and unablated females after spawning. The results also demonstrate the effects of unilateral eyestalk ablation in the reproductive cycle of F. paulensis under laboratory conditions.
References
BELL, T.A. and LIGHTNER, D.V. 1988 A Handbook of Normal Penaeid Shrimp Histology. Baton Rouge:Special Publication of the World Aquaculture Society. 114p.
BROWDY, C.L. 1992 A review of the reproductive biology of Penaeus species; perspectives on controlled shrimp maturation systems for high quality nauplii production. In: SPECIAL SESSION ON SHRIMP FARMING, Baton Rouge, 1992. Proceedings... Baton Rouge, World Aquaculture Society. p.22-51.
________ and SAMOCHA, T.M. 1985 The effect of eyestalk ablation on the spawning, molting and mating of Penaeus semisulcatus de Haan.Aquaculture, 49: 19-49.
CAVALLI, R.O.; SCARDUA, M.P.; WASIELESKY, W. 1997 Reproductive performance of different sized wild and
pond-reared Penaeus paulensis females. Journal of the World Aquaculture Society, 28: 260-267.
DALL, W.; HILL, B.J.; ROTHLISBERG, P.C.; STAPLES, D.J.1990 The Biology of the Penaeidae. Advances in Marine Biology. London: Academic Press. 489p.
EMMERSON, W.D. 1980 Induced Maturation of Prawn Penaeus indicus. Marine Ecology Progress Series,2: 121-131.
PEIXOTO et al.
KELEMEC, J.A. and SMITH, I.R. 1984 Effects of low temperature storage and eyestalk enucleation of gravid eastern king prawns, Penaeus plebejus, on spawning, egg fertilization and hatching. Aquaculture, 40: 67-76.
KING, J.E. 1948 A study of the reproductive organs of the common marine shrimp, Penaeus setiferus (Linnaeus). Biological Bulletin Marine Laboratory,94: 244-262.
KULKARNI, G.K. and NAGABHUSHANAM, R. 1980 Role of ovary-inhibiting hormone from eyestalks of marine penaeid prawns (Parapenaeopsis hardwickii) during the ovarian development cycle. Aquaculture, 19: 13-19.
MARCHIORI, M.A. and BOFF, M.H. 1983 Induced maturation, spawning and larvae culture of the pink shrimp
Penaeus paulensis Pérez-Farfante, 1967. Memorias de la Asociación Latino-americana de Acuicultura,5: 331-337.
________ and CAVALLI, R.O. 1993 Commercial scale maturation of Penaeus paulensis in a semi-closed recirculation system. In: BRAZILIAN SYMPOSIUM ON SHRIMP CULTURE, 4, João Pessoa, 1993. Proceedings... João Pessoa, MCR Aquicultura Brazil. p. 385-398.
MARTOSUBROTO, P. 1974 Fecundity of pink shrimp, Penaeus duorarum Burkenroad. Bulletin of Marine Science, 24: 606-627.
MEDINA, A.; VILA, Y.; MOURENTE, G.; RODRÍGUEZ, A. 1996 A comparative study of the ovarian development in wild and pond-reared shrimp, Penaeus kerathurus (Forskal, 1775).Aquaculture, 148: 63-75.
QUINTERO, M.E.S. and GARCIA, A. 1998 Stages of gonadal development in the spotted pink shrimp Penaeus brasiliensis. Journal of Crustacean Biology, 18: 680-685.
PEIXOTO, S.; D‘INCAO, F.; WASIELESKY, W.; POERSCH, L.H. 1998 Análise da viabilidade reprodutiva do camarão-rosa Penaeus paulensis em laboratório sem a utilização da técnica de ablação do pedúnculo ocular de fêmeas. In: REUNIÃO ANUAL DO INSTITUTO DE PESCA, 7, São Paulo, 1998. Resumos... São Paulo, Instituto de Pesca/SAA. p. 82.
________; WASIELESKY, W.; D‘INCAO, F.; CAVALLI,R.O. 2002 Reproductive performance of similarly-sized wild and captive Farfantepenaeus paulensis. Journal of the World Aquaculture Society (in press).
TAN-FERMIN, J.D. 1991 Effects of unilateral eyestalk
ablation on ovarian histology and oocyte size frequency of wild and pond-reared Penaeus monodon
(Fabricius) broodstock. Aquaculture, 93: 77-86.
________ and PUDADERA, R.A. 1989 Ovarian maturation stages of the wild giant tiger prawn Penaeus
monodon Fabricius. Aquaculture, 77: 229-242.
VOGT, G.; QUINITIO, E.T.; PASCUAL, F.P. 1989 Interaction of the midgut gland and the ovary in vitellogenesis and consequences for the breeding success: a comparison of unablated and ablated spawners of Penaeus monodon. In: INTERNATIONAL AQUACULTURE EUROPE, Amsterdam, 1989. Proceedings...
Amsterdam. p.581-592.
WORSMANN, T.U. and SESSO, A. 1977 Observations ultrastructurales sur la maturation des cellules germinales de l’ovaire de Penaeus paulensis. Archives D’Anatomie D’Histologie D’Embryologie Normales et Expérimentales, 60: 175-188.
________; NEIVA, G.S.; OLIVEIRA, M.T.; VALENTINI, H.1971 Contribuição ao estudo da maturação da gônada feminina do "camarão-rosa†(Penaeus paulensis Perez Farfante, 1967). B. Inst. Pesca,1: 23-28.
