Spermatozoa evaluation and cryopreservation in pellets fron of "bagre" Rhamdia hilarii (Valenciennes, 1840) sêmen

Authors

  • Washington FOGLI DA SILVEIRA Pesquisador Cientí­­ficos - Seção de Biologia Aquática-Divisão de Pesca Interior de Pesca
  • Emico Tahira KAVAMOTO Pesquisador Cientí­­ficos - Seção de Biologia Aquática-Divisão de Pesca Interior de Pesca
  • Massuka Yamane NARAHARA Pesquisador Cientí­­ficos - Seção de Biologia Aquática-Divisão de Pesca Interior de Pesca

Keywords:

NíO CONSTA

Abstract

The spermatic characteristics and the criopreservation of 10 and 6 "bagres", Rhamdia hilarii, sperm respectively were studied with the purpose to maintain a frozen semen bank for artificial fertilization of freshwater fishes of economical value. This paper was carried out at Laboratório de Biologia Aquática do Instituto de Pesca (São Paulo-Brazil). The average values of spermatic characteristics were: semen volume, 1.15 ml; spermatozoa concentration, 55.80 x 106/mm3; spermatozoa motility, 72.00%; and percentage of live spermatozoa by differential staining method, 76.09%. The semen of each male, mixed with extender at ratio of 1:3, was frozen in pellets form on dry ice (-79°C) and storage in liquid nitrogen (-196°C). Samples thawed and examined under phase-contrast microscopy presented an average of 31.94% live spermatozoa. Spermatozoa showed 30% motility 36 months after freezing.

 

Published

2018-06-28

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