Diluentes e proporções sêmen:diluente na crioconservação do sêmen de robalo-peva Centropomus parallelus
Palavras-chave:
robalo-peva, Centropomus parallelus, sêmen, crioconservação, diluente, tanque-redeResumo
Com a finalidade de desenvolver um protocolo de crioconservação do sêmen do robalo-peva, Centropomus parallelus, foram realizados, no Instituto de Pesca/SAA-SP, estudos para otimizar a motilidade e tempo de motilidade espermáticas e a capacidade de fecundação do sêmen. Foram utilizados 50 reprodutores oriundos de reprodução artificial e criados em tanque-rede instalado em ambiente oceí¢nico. O sêmen, diluído em três soluções de Ringer (pH 6,1; 7,8 e 8,2) nas proporções sêmen:diluente de 1:1, 1:2, 1:3, 1:4 e 1:5, foi congelado em vapor de nitrogênio e, posteriormente, transferido para nitrogênio líquido. A velocidade de congelamento, a concentração do crioprotetor dimetilsulfóxido (DMSO) e o tempo de equilíbrio foram, respectivamente, 90°C min-1, 10% e 60 segundos. A capacidade de fecundação foi determinada calculando-se volumes de sêmen para obtenção das concentrações 4x104; 1x105; 1,6x105 e 2,2x105 espermatozoides:ovócito. Para desova, as fêmeas receberam gonadotrofina coriônica humana (hCG) na dose 1 UI/g.peso. Para o sêmen fresco, a motilidade foi de 100%, o tempo de motilidade, de 464,6 ± 47,56 s. Para o sêmen crioconservado, os melhores resultados foram motilidade de 70,0 ± 3,94% e tempo de motilidade de 610,8 ± 9,60 s, com o diluente de pH 8,2. Não foram observadas diferenças significativas (p<0,01) em relação í s diferentes proporções sêmen:diluente. Foram obtidas taxas de fecundação (99,0 ± 0,66%) iguais (p<0,01) a partir da concentração 1,6x105 espermatozoides:ovócito, indicando que a crioconservação constituiu-se em recurso importante para estoque de sêmen e controle da reprodução do robalo-peva.
Referências
ALAVI, S.M.H.; COSSON, J.; KARAMI, M.;ABDOULHAY, H.; MOZAJI AMIRI, B. 2004
Chemical composition and osmolality of seminal plasma of Acipenser persicus, their
physiological relationsheap with sperm motility. Aquac. Res., 35: 1238-1243.
ALVAREZ-LAJONCHÉRE, L. y MOLEJÓN, O.G.H. 2001 Producción de juveniles de peces estuarinos para un centro en América Latina y el Caribe: diseí±o,operación y tecnologías. The World Aquaculture Society, Baton Rouge, LA. 424p.
BILLARD, R. 1978 Some data on gametes preservation and artificial insemination in teleost fish. Actes de colloques du CNEXO 8: 59-73.
BILLARD, R. 1990 Artificial insemination in fish. In:LAMMING, G.E. Marshall’s physiology of reproduction. Churchill Livingstone: Edinburg,2: 870-888.
BILLARD, R. and COSSON, J. 1988 Sperm motility in rainbow trout, Parasalmo gairdneri: effects of pH and temperature. In Breton B. and Zohar Y. eds. Reproduction in fish basic and applied aspects in endocrinology and genetics. Paris:
INRA. p. 161-167.
BILLARD, R.; COSSON, J.; CRIM, L.W. 1993 Motility of fresh and aged halibut sperm. Aquatic Living Resources, 6: 67-75.
BILLARD, R.; COSSON, J.; CRIM, L.M.; SUQUET, M.1996 Sperm physiology and quality. In:BROMAGE N.R. and ROBERTS, R.J. Broodstock management and egg and larval quality. Oxford:Blackwell Science. p. 25-52.
BLAXTER, J.H.S. 1953 Sperm storage and crossfertilization of spring and autumn spawning herring. Nature, 172: 1189-1190.
CHAO, N.H.; CHEN, H.P.; LIAO, I.C. 1975 Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5: 389-406.
CHAO, N.H.; TSAI, H.P.; LIAO, I.C. 1992 Short and long-term cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Schneider). Asian Fishery Science, 5: 103-116.
CIERESZKO, A.; Glogowski, J.; Dabrowski, K. 2000 Biochemical characteristics of seminal plasma and spermatozoa. In: TIERSCH, T.R. and MAZIC, P.M. Cryopreservation in aquatic species.World Aquaculture Society, Baton Rouge,
Louisiana. p.20-48.
COSSON, J. 2004 The ionic and osmotic factors controlling motility of fish spermatozoa.Aquac. Int. 12: 69-85.
COSSON, J.; GROISON, A.-L.; SUQUET, M.; FAUVEL, C.;DREANNO, C.; BILLARD, R. 2008a Studing sperm motility in marine fish: an overview on the state of the art. J. Appl. Ichthyol. 24: 460-486.
COSSON, J.; GROISON, A.-L.; SUQUET, M.; FAUVEL, C.;DREANNO, C.; BILLARD, R. 2008b Marine fish spermatozoa: racing ephemeral swimmers.Reproduction. 136: 277-294.
DREANNO, C.; SUQUET, M.; QUEMENER, L.; COSSON, J.; FIERVILLE, F.; NORMANT, Y.; BILLARD, R.1997 Cryopreservation of turbot (Scophthalmus maximus) spermatozoa. Theriogenology, 48: 589-603.
FAUVEL, C.; SUQUET, M.; DREANNO, C.; ZONNO, V.; MENU, B. 1998 Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions. Aquat. Living Resour., 11: 387-394.
FAUVEL, C.; SAVOYE, O.; DREANNO, C.; COSSON, J.;SUQUET, M. 1999 Characteristics of captive seabass in relation to its fertilization potential.Journal of Fish Biology, 54: 356-369.
FERRAZ, E.M.; CERQUEIRA, V.R.; ALVAREZLAJONCHíˆRE, L.; CANDIDO, S. 2003. Indução í desova do robalo-peva, Centropomus parallelus, através de injeção e implante de LHRHa. Bol. Inst. Pesca, São Paulo, 28(2): 125-133.
GARZÓN, D.L.; PEí"˜ARANDA, D.S.; PÉREZ, L.;MARCO-JIMÉNEZ, F.; ESPERt, X.; MíÅ“LLER, T.; JOVER, M.; ASTURIANO, J.F. 2008 Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cyopreservation of european eel sperm. Reprod. Dom Anim., 43:99-105.
GODINHO, H. M.; SERRALHEIRO, P. C. S.; FERRAZ,E.M.; PIMENTEL, C. M. M.; OLIVEIRA, I.R. e PAIVA, P. 2000 Reprodução induzida em robalo Centropomus parallelus Poey, 1860. Braz.J.Res.anim.Sci., São Paulo, 37(1): 37-42.
GWO, J.C. 2000 Cryopreservation of sperm of some marine fishes. In: TIERSCH, T.R. and MAZIC, P.M. Cryopreservation in aquatic species. World Aquaculture Society, Baton Rouge,Louisiana. p.138-160.
INABA, K, 2003 Molecular architecture of the sperm flagella: molecules for motility and signaling. Zool. Sci., 20: 1043-1056.
LEE, H.C.; HOHNSON C.; EPEL, D. 1983 Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm. Dev. Biol., 95: 31-45.
LAHNSTEINER, F.; WEISMANN, T.; PATZNER, R.A. 1997 Metanol as cryoprotectant and the suitability of 1.2 mL and 5 mL straws for cryopreservation of semen from salmonid fishes. Aquaculture Reserch, Salzburg, 28: 471-479.
LEUNG, L. K. P. 1987 Cryopreservation of spermatozoa of the barramundi, Lates
calcarifer (Teleostei: Centropomidae).Aquaculture, Amsterdam, 64: 243-247.
LUBZENS, E.; DAUBE, N.; PEKARSDY, I.; MAGNUS,Y.;COHEN, A.; YUSEFOVICH, F.; FEIGIN, P. 1997 Carp (Cyprinus carpio L.) spermatozoa cryobanks: strategies in research and application. Aquaculture, Amsterdam, 155: 13-30.
Mí€RIí€N, T.; KRASZNAI, Z.; BALKAY, L.; EMRI, M.;TRÓN, L. 1997 Role of extracellular and intracellular pH in carp sperm motility and modifications by hyperosmosis of regulation of the Na+/H+ exchanger. Cytometry, 27: 374-382.
MC ANDREW, B.J.; RANA, K.J.; PENMAN, D.J. 1993 Conservation and preservation of genetic variation in aquatic organism. In: MUIR, J.F. and ROBERTS, R.J. Recent Advances in: Aquaculture, vol IV, Oxford, Blackwell. p.295-336.
MEDINA, V.; VELASCO, Y.; CRUZ, P. 2005 Aspectos generales de la criopreservation espermática en peces teleósteos. Rev. Colomb. Cienc.Pecuar., 18: 34-48.
MIOSO, R. 1995 Indução í reprodução e incubação de ovos de robalo Centropomus parallelus Poey, 1860 (Pisces, Centropomidae). Florianópolis. 51p.(Dissertação de Mestrado. Universidade Federal de Santa Catarina).
MOUNIB, M.S.; HWANG, P.C.; IDLER, D.R. 1968 Cryogenic preservation of Atlantic cod Gadus morhua sperm. Journal of the Fisheries,Canada, 25: 2623-2632.
ODA, S. and MORISAWA, M. 1993a Rises of intracellular Ca+ and pH mediate the
initiation of sperm motility in marine teleosts. Cell Motil. Cytoskeleton. 25: 171-178.
ODA, S. and MORISAWA, M. 1993b Rises of intracellular Ca+ and pH mediate the
initiation of sperm of farmed european eel Anguilla Anguilla. J. World Aquac. Soc. 35:
240-246.
PELETEIRO, J.B.; CHEREGUINI, O.; CAL, R.M. 1996 Preliminary results of artificial fertilization carried out with cryopreserved sperm of turbot Scophthalmus maximus (Linnaeus, 1758). Informe Tecnico del Instituto Espanological de Oceanografo, 162: 1-13.
RANA, K.J.; GUPTA, S.D.; MCANDREW, B.J. 1992 The relevance of collection techniques on the quality of manually stripped Atlantic salmon (Salmo salar) milt. In: WORKSHOP ON GAMETE AND EMBRYO STORAGE AND CRYOPRESERVATION IN AQUATIC ORGANISMS. 30 march í 2 april/1992.
RANA, K.J. 1996 Preservation of gametes. In:BROMAGE, N.R. and ROBERTS,R.J. Broodstock management and egg and larval quality.Oxford: Blackwell Science, p.53-75.
RIDEOUT, R.M.; TRIPPEL, E.A.; LITVAK, M.K. 2004 The development of haddock and Atlantic cod sperm cryopreservation techniques and effect of sperm age on cryopreservation success. Journal of Fish Biology, St. John’s, 65:299-311.
RILEY, K.L.; HOLLADAY, C.G.; CHESNEY, E.J.;TIERSCH, T.R. 2004 Cryopreservation of sperm of red snapper. Aquaculture, 238: 183-194.
SANCHES, E.G.; OLIVEIRA, I.R.; SERRALHEIRO, P.C.S.2008 Crioconservação do sêmen da garoupaverdadeira Epinephelus marginatus. Bioikos, 22 (2): 81-90.
SANSONE, G.; FABRIOCINI, A.; IEROPOLI, S.;LANGELLOTTI, A.L.; OCCIDENTE, M.;
MATASSINO, D. 2002 Effects of extender composition, cooling rate, and freezing on the motility of sea bass (Dicentrarchus labrax, L.) spermatozoa after thawing. Cryobiology,Elsevier Science, Napoli, 44: 229-239.
SCOTT, A.P. and BAYNES, S.M. 1980 A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol., 17: 707-739.
SERRALHEIRO, P.C.S.; OLIVEIRA, I.R.; GODINHO,H.M. 1998 Fertilização de ovócitos de robalo Centropomus parallelus, com sêmen crioconservado. In: SIMPÓSIO BRASILEIRO DE AQíÅ“ICULTURA, 12, Recife, 1998. Anais…Recife, p.360-1.
SERRALHEIRO, P.C. DA S.; FOGLI DA SILVEIRA,W.;GODINHO, H.M.; OLIVEIRA, I DA R. 1999. O uso de três soluções diluidoras em sêmen de tainha í Mugil platanus, Günther, 1880,resfriado em container de vapor de nitrogênio líquido. In: ENCONTRO BRASILEIRO DE ICTIOLOGIA, 13, São Carlos. Anais... p.508.
STOSS, J. 1983 Fish gamete preservation and spermatozoan physiology. In: HOAR, W.S.; RANDALL, D.J.; DONALDSON, E.M. (Eds.). Fish physiology. New York: Academic Press.p.305-350.
SUQUET, M. 1992 La production de sperme chez le turbot, aspects descriptifs et experimentaux. Mémorie EPHE, Paris.
SUQUET, M.; DREANNO, C.; FAUVEL, C.; COSSON,J.; BILLARD, R. 2000 Cryopreservation ofsperm in marine fish. Aquaculture Research,31: 231-243.
TANAKA, S.; ZANG, H.; RORIE, N.; YAMADA, Y.; OKAMURA, A.; UTOH, T., MIKAWA, N.; OKA, H.P.; KUROKURA, H. 2002 Long-term cryopreservation of sperm of Japanese eel. J.Fish Biol. 60: 139-146.
TIERSCH, T.R. 2000 Introduction. In: TIERCH, T.R.and MAZUR P.M. Cryopreservation in Aquatic Species. World Aquaculture Society. Baton Rouge, Louisiana. p.xix-xxvi.
TIERSCH, T.R.; WAYMAN, W.R.; SKAPURA, D.P.;NEIDIG, C.L.; GRIER, H.J. 2004 Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Bloch). Aquaculture Research. Baton Rouge, Louisiana,35: 278-288.
ZILLI, L.; SCHIAVONE, R.; ZONNO, V.; ROSSANO, R.;STORELLI, C.; VILELLA, S. 2005 Effect of cryopreservation on sea bass sperm proteins.Biology of Reproduction, Lecce, 72: 1262-1267.
Chemical composition and osmolality of seminal plasma of Acipenser persicus, their
physiological relationsheap with sperm motility. Aquac. Res., 35: 1238-1243.
ALVAREZ-LAJONCHÉRE, L. y MOLEJÓN, O.G.H. 2001 Producción de juveniles de peces estuarinos para un centro en América Latina y el Caribe: diseí±o,operación y tecnologías. The World Aquaculture Society, Baton Rouge, LA. 424p.
BILLARD, R. 1978 Some data on gametes preservation and artificial insemination in teleost fish. Actes de colloques du CNEXO 8: 59-73.
BILLARD, R. 1990 Artificial insemination in fish. In:LAMMING, G.E. Marshall’s physiology of reproduction. Churchill Livingstone: Edinburg,2: 870-888.
BILLARD, R. and COSSON, J. 1988 Sperm motility in rainbow trout, Parasalmo gairdneri: effects of pH and temperature. In Breton B. and Zohar Y. eds. Reproduction in fish basic and applied aspects in endocrinology and genetics. Paris:
INRA. p. 161-167.
BILLARD, R.; COSSON, J.; CRIM, L.W. 1993 Motility of fresh and aged halibut sperm. Aquatic Living Resources, 6: 67-75.
BILLARD, R.; COSSON, J.; CRIM, L.M.; SUQUET, M.1996 Sperm physiology and quality. In:BROMAGE N.R. and ROBERTS, R.J. Broodstock management and egg and larval quality. Oxford:Blackwell Science. p. 25-52.
BLAXTER, J.H.S. 1953 Sperm storage and crossfertilization of spring and autumn spawning herring. Nature, 172: 1189-1190.
CHAO, N.H.; CHEN, H.P.; LIAO, I.C. 1975 Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5: 389-406.
CHAO, N.H.; TSAI, H.P.; LIAO, I.C. 1992 Short and long-term cryopreservation of sperm and sperm suspension of the grouper, Epinephelus malabaricus (Bloch and Schneider). Asian Fishery Science, 5: 103-116.
CIERESZKO, A.; Glogowski, J.; Dabrowski, K. 2000 Biochemical characteristics of seminal plasma and spermatozoa. In: TIERSCH, T.R. and MAZIC, P.M. Cryopreservation in aquatic species.World Aquaculture Society, Baton Rouge,
Louisiana. p.20-48.
COSSON, J. 2004 The ionic and osmotic factors controlling motility of fish spermatozoa.Aquac. Int. 12: 69-85.
COSSON, J.; GROISON, A.-L.; SUQUET, M.; FAUVEL, C.;DREANNO, C.; BILLARD, R. 2008a Studing sperm motility in marine fish: an overview on the state of the art. J. Appl. Ichthyol. 24: 460-486.
COSSON, J.; GROISON, A.-L.; SUQUET, M.; FAUVEL, C.;DREANNO, C.; BILLARD, R. 2008b Marine fish spermatozoa: racing ephemeral swimmers.Reproduction. 136: 277-294.
DREANNO, C.; SUQUET, M.; QUEMENER, L.; COSSON, J.; FIERVILLE, F.; NORMANT, Y.; BILLARD, R.1997 Cryopreservation of turbot (Scophthalmus maximus) spermatozoa. Theriogenology, 48: 589-603.
FAUVEL, C.; SUQUET, M.; DREANNO, C.; ZONNO, V.; MENU, B. 1998 Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions. Aquat. Living Resour., 11: 387-394.
FAUVEL, C.; SAVOYE, O.; DREANNO, C.; COSSON, J.;SUQUET, M. 1999 Characteristics of captive seabass in relation to its fertilization potential.Journal of Fish Biology, 54: 356-369.
FERRAZ, E.M.; CERQUEIRA, V.R.; ALVAREZLAJONCHíˆRE, L.; CANDIDO, S. 2003. Indução í desova do robalo-peva, Centropomus parallelus, através de injeção e implante de LHRHa. Bol. Inst. Pesca, São Paulo, 28(2): 125-133.
GARZÓN, D.L.; PEí"˜ARANDA, D.S.; PÉREZ, L.;MARCO-JIMÉNEZ, F.; ESPERt, X.; MíÅ“LLER, T.; JOVER, M.; ASTURIANO, J.F. 2008 Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cyopreservation of european eel sperm. Reprod. Dom Anim., 43:99-105.
GODINHO, H. M.; SERRALHEIRO, P. C. S.; FERRAZ,E.M.; PIMENTEL, C. M. M.; OLIVEIRA, I.R. e PAIVA, P. 2000 Reprodução induzida em robalo Centropomus parallelus Poey, 1860. Braz.J.Res.anim.Sci., São Paulo, 37(1): 37-42.
GWO, J.C. 2000 Cryopreservation of sperm of some marine fishes. In: TIERSCH, T.R. and MAZIC, P.M. Cryopreservation in aquatic species. World Aquaculture Society, Baton Rouge,Louisiana. p.138-160.
INABA, K, 2003 Molecular architecture of the sperm flagella: molecules for motility and signaling. Zool. Sci., 20: 1043-1056.
LEE, H.C.; HOHNSON C.; EPEL, D. 1983 Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm. Dev. Biol., 95: 31-45.
LAHNSTEINER, F.; WEISMANN, T.; PATZNER, R.A. 1997 Metanol as cryoprotectant and the suitability of 1.2 mL and 5 mL straws for cryopreservation of semen from salmonid fishes. Aquaculture Reserch, Salzburg, 28: 471-479.
LEUNG, L. K. P. 1987 Cryopreservation of spermatozoa of the barramundi, Lates
calcarifer (Teleostei: Centropomidae).Aquaculture, Amsterdam, 64: 243-247.
LUBZENS, E.; DAUBE, N.; PEKARSDY, I.; MAGNUS,Y.;COHEN, A.; YUSEFOVICH, F.; FEIGIN, P. 1997 Carp (Cyprinus carpio L.) spermatozoa cryobanks: strategies in research and application. Aquaculture, Amsterdam, 155: 13-30.
Mí€RIí€N, T.; KRASZNAI, Z.; BALKAY, L.; EMRI, M.;TRÓN, L. 1997 Role of extracellular and intracellular pH in carp sperm motility and modifications by hyperosmosis of regulation of the Na+/H+ exchanger. Cytometry, 27: 374-382.
MC ANDREW, B.J.; RANA, K.J.; PENMAN, D.J. 1993 Conservation and preservation of genetic variation in aquatic organism. In: MUIR, J.F. and ROBERTS, R.J. Recent Advances in: Aquaculture, vol IV, Oxford, Blackwell. p.295-336.
MEDINA, V.; VELASCO, Y.; CRUZ, P. 2005 Aspectos generales de la criopreservation espermática en peces teleósteos. Rev. Colomb. Cienc.Pecuar., 18: 34-48.
MIOSO, R. 1995 Indução í reprodução e incubação de ovos de robalo Centropomus parallelus Poey, 1860 (Pisces, Centropomidae). Florianópolis. 51p.(Dissertação de Mestrado. Universidade Federal de Santa Catarina).
MOUNIB, M.S.; HWANG, P.C.; IDLER, D.R. 1968 Cryogenic preservation of Atlantic cod Gadus morhua sperm. Journal of the Fisheries,Canada, 25: 2623-2632.
ODA, S. and MORISAWA, M. 1993a Rises of intracellular Ca+ and pH mediate the
initiation of sperm motility in marine teleosts. Cell Motil. Cytoskeleton. 25: 171-178.
ODA, S. and MORISAWA, M. 1993b Rises of intracellular Ca+ and pH mediate the
initiation of sperm of farmed european eel Anguilla Anguilla. J. World Aquac. Soc. 35:
240-246.
PELETEIRO, J.B.; CHEREGUINI, O.; CAL, R.M. 1996 Preliminary results of artificial fertilization carried out with cryopreserved sperm of turbot Scophthalmus maximus (Linnaeus, 1758). Informe Tecnico del Instituto Espanological de Oceanografo, 162: 1-13.
RANA, K.J.; GUPTA, S.D.; MCANDREW, B.J. 1992 The relevance of collection techniques on the quality of manually stripped Atlantic salmon (Salmo salar) milt. In: WORKSHOP ON GAMETE AND EMBRYO STORAGE AND CRYOPRESERVATION IN AQUATIC ORGANISMS. 30 march í 2 april/1992.
RANA, K.J. 1996 Preservation of gametes. In:BROMAGE, N.R. and ROBERTS,R.J. Broodstock management and egg and larval quality.Oxford: Blackwell Science, p.53-75.
RIDEOUT, R.M.; TRIPPEL, E.A.; LITVAK, M.K. 2004 The development of haddock and Atlantic cod sperm cryopreservation techniques and effect of sperm age on cryopreservation success. Journal of Fish Biology, St. John’s, 65:299-311.
RILEY, K.L.; HOLLADAY, C.G.; CHESNEY, E.J.;TIERSCH, T.R. 2004 Cryopreservation of sperm of red snapper. Aquaculture, 238: 183-194.
SANCHES, E.G.; OLIVEIRA, I.R.; SERRALHEIRO, P.C.S.2008 Crioconservação do sêmen da garoupaverdadeira Epinephelus marginatus. Bioikos, 22 (2): 81-90.
SANSONE, G.; FABRIOCINI, A.; IEROPOLI, S.;LANGELLOTTI, A.L.; OCCIDENTE, M.;
MATASSINO, D. 2002 Effects of extender composition, cooling rate, and freezing on the motility of sea bass (Dicentrarchus labrax, L.) spermatozoa after thawing. Cryobiology,Elsevier Science, Napoli, 44: 229-239.
SCOTT, A.P. and BAYNES, S.M. 1980 A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol., 17: 707-739.
SERRALHEIRO, P.C.S.; OLIVEIRA, I.R.; GODINHO,H.M. 1998 Fertilização de ovócitos de robalo Centropomus parallelus, com sêmen crioconservado. In: SIMPÓSIO BRASILEIRO DE AQíÅ“ICULTURA, 12, Recife, 1998. Anais…Recife, p.360-1.
SERRALHEIRO, P.C. DA S.; FOGLI DA SILVEIRA,W.;GODINHO, H.M.; OLIVEIRA, I DA R. 1999. O uso de três soluções diluidoras em sêmen de tainha í Mugil platanus, Günther, 1880,resfriado em container de vapor de nitrogênio líquido. In: ENCONTRO BRASILEIRO DE ICTIOLOGIA, 13, São Carlos. Anais... p.508.
STOSS, J. 1983 Fish gamete preservation and spermatozoan physiology. In: HOAR, W.S.; RANDALL, D.J.; DONALDSON, E.M. (Eds.). Fish physiology. New York: Academic Press.p.305-350.
SUQUET, M. 1992 La production de sperme chez le turbot, aspects descriptifs et experimentaux. Mémorie EPHE, Paris.
SUQUET, M.; DREANNO, C.; FAUVEL, C.; COSSON,J.; BILLARD, R. 2000 Cryopreservation ofsperm in marine fish. Aquaculture Research,31: 231-243.
TANAKA, S.; ZANG, H.; RORIE, N.; YAMADA, Y.; OKAMURA, A.; UTOH, T., MIKAWA, N.; OKA, H.P.; KUROKURA, H. 2002 Long-term cryopreservation of sperm of Japanese eel. J.Fish Biol. 60: 139-146.
TIERSCH, T.R. 2000 Introduction. In: TIERCH, T.R.and MAZUR P.M. Cryopreservation in Aquatic Species. World Aquaculture Society. Baton Rouge, Louisiana. p.xix-xxvi.
TIERSCH, T.R.; WAYMAN, W.R.; SKAPURA, D.P.;NEIDIG, C.L.; GRIER, H.J. 2004 Transport and cryopreservation of sperm of the common snook, Centropomus undecimalis (Bloch). Aquaculture Research. Baton Rouge, Louisiana,35: 278-288.
ZILLI, L.; SCHIAVONE, R.; ZONNO, V.; ROSSANO, R.;STORELLI, C.; VILELLA, S. 2005 Effect of cryopreservation on sea bass sperm proteins.Biology of Reproduction, Lecce, 72: 1262-1267.
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2018-11-04
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