Toxicity and effects of copper sulfate on parasitic control and hematological response of tambaqui Colossoma macropomum


  • Marcos TAVARES-DIAS Laboratório de Aquicultura e Pesca, Embrapa Amapá
  • Jessé Santos FERREIRA Laboratório de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas (UFAM)
  • Elizabeth Gusmão AFFONSO Laboratório de Fisiologia Aplicada Á  Piscicultura, Instituto Nacional de Pesquisa da Amazônia (INPA)
  • Eduardo Akifumi ONO Laboratório de Fisiologia Aplicada Á  Piscicultura, Instituto Nacional de Pesquisa da Amazônia (INPA)
  • Maurício Laterça MARTINS Departamento de Aquicultura, Universidade Federal de Santa Catarina (UFSC)


blood, freshwater fish, leukocytes, parasites, Monogenoidea


The purpose of this work was to determine the mean lethal concentration (LC50-96h) of copper sulfate (CuSO4) for juveniles Colossoma macropomum (Characidae), to evaluate the effectiveness of different concentrations of CuSO4 on elimination of parasites, as well as to study the effects of CuSO4 on blood parameters after short-term exposure. After determination of the LC50-96h from 17.5 mg L-1 of CuSO4, it was investigated the effects of exposure for 48 h to 1.75, 4.37 and 8.75 mg L-1 of CuSO4 on parasites and blood parameters. In gills and skin, concentrations of 4.37 and 8.75 mg L-1 of CuSO4 eliminated Monogenoidea Anacanthorus spathulatus. In fish exposed to different concentrations of CuSO4, plasma total protein, chloride, potassium, glucose and copper levels, hemoglobin and hematocrit were similar to controls, whereas sodium levels presented decrease in fish exposed to 1.75 and 4.37 mg L-1. Red blood cell counts decreased in fish exposed to different three concentrations of CuSO4, while the Mean Corpuscular Volume (MCV) increased. However, exposure to 8.75 mg L-1 of CuSO4 caused decrease on total leukocytes, lymphocytes, neutrophils and PAS-positive granular leukocytes (PAS-GL) number when compared to control fish.


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