Isolation and antimicrobial susceptibility of bacteria from injuries caused by ictio in jundia (Rhamdia quelen)
DOI:
https://doi.org/10.20950/1678-2305.2016v42n1p195Keywords:
Ichthyophthirius multifiliis, South American silver catfish, bacterial infection, antimicrobials in aquaculture, antimicrobial resistanceAbstract
Ichthyophthirius multifiliis is the cause of a parasitic disease that affects freshwater fish, being one of the major complications the rise of secondary lesions caused by bacteria. In this context, the aim of this study was to identify the micro-organisms present in the skin lesions of jundias (Rhamdia quelen) infected by ictio and verify the antimicrobial susceptibility of the isolates. Infected skin portions were collected, followed by direct plating on 8% sheep blood agar and MacConkey with further identification in grampositive and gram-negative bacteria. Escherichia coli (1/10), Burkholderia pseudomallei (2/10), Morganella morganii (2/10), and Aeromonas hydrophila (5/10) were isolated. Among the antimicrobials tested, resistance was only observed to ampicillin. In conclusion, the agents isolated in this study can compromise the performance parameters of fish production. In addition, the presence of such micro-organisms suggests a risk to public health with respect to the consumption of meat containing toxic substances or microorganisms resistant to important antimicrobial classes in human medicine care.
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PROCÓPIO, R.C.O. 2000. Ocorrência de bactérias formadoras de histamina em tunídeos utilizados para enlatamento. Niterói, Brasil, Rio de Janeiro. 91f. (Tese de doutorado, Universidade Federal Fluminense). Disponível em:< http://www.ndc.uff.br/content/biblioteca-digital-de-teses-e-disserta%C3%A7%C3%B5es>. Acesso em: 18 abril 2014.
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SPRAGUE, L.D and NEUBAUER, H. 2004. Melioidosis in animals: a review on epizootiology, diagnosis and clinical presentation. Journal of Veterinary Medicine, Series B. 51:305-320.
WEINSTEIN, M.; LOW, D.; MCGEER, A.; WILLEY, B.; ROSE, D.; COULTER, M.; WYPER ,P.; BORCZYK, A.; LOVGREN, M.; FACKLAM, R. 1996. Invasive infection due to Streptococcus iniae: a new or previously unrecognized disease - Ontario.Canada Communicable Disease Report. 22:129-131.
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XU, D.H.; PRIDGEON, J.W.; KLESIUS, P.H.; SHOEMAKER, C.A. 2012. Parasitism by protozoan Ichthyophthirius multifiliis enhanced invasion of Aeromonas hydrophila in tissues of channel catfish. Veterinary Parasitology. 184:101-107.
AGNESE, A.P.; OLIVEIRA, V.M.; SILVA, P.P.O.; OLIVEIRA, G.A. 2001. Contagem de bactérias heterotróficas aeróbias mesófilas e numeração de coliformes totais e fecais em peixes frescos comercializados no município de Seropédicaí RJ. Higiene Alimentar. 15: 67-70.
AKINBOWALE, O.L.; PENG, H.; BARTON, M.D. 2007. Diversity of tetracycline resistance genes in bacteria from aquaculture sources in Australia. Journal Applied Microbiology. 103: 2016-2025.
BALDISSEROTTO, B. 2009. Pscicultura continental no Rio Grande do Sul: situação atual, problemas e perspectivas para o futuro. Ciência Rural. 39: 291-299.
BARBOSA, M.M.C.; PINTO, F.R.; RIBEIRO, L.F.; GURIZ, C.S.L.; FERRAUDO, A.S.; MALUTA, R.P.; RIGOBELO, E.C.; ÁVILA, F.A.; AMARAL, L.A. 2014. Serology and patterns of antimicrobial susceptibility in Escherichia coli isolates from pay-to-fish ponds. Food Safety. Arquivos do Instituto Biológico.81: 43-48.
BOIJINK, C.L. e BRANDÃO, D.A. 2001. Alterações histológicas e comportamentais provocadas pela inoculação de suspensão bacteriana (Aeromonas hydrophila) em juvenis de jundiá (Rhamdia quelen). Ciência Rural. 31:687-690.
BORBA, M.R.; FRACALOSSI, D.M.; FREITAS, F.A. 2007. Efeito da suplementação de vitamina C na dieta sobre a susceptibilidade de alevinos de Jundiá, Rhamdia quelen, ao Ichthyophthirius multifiliis. Acta Scientiarum. Animal Science.29: 93-99.
CABELLO, F.C. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the Environment Microbiology.8: 1137-1144.
CARNEIRO, P.C.F.; SCHORER, M.; MIKOS, J.D. 2005. Tratamentos terapêuticos convencionais no controle do ectoparasita Ichthyophtirius multifiliis em jundiá (Rhamdia quelen). Pesquisa Agropecuária Brasileira. 40: 99-102.
CARNEVIA, D., PERRETTA, A.; LETAMENDÍA, M.; DELGADO, E. 2010. Enfermedades diagnosticadas en organismos acuáticos de cultivo en Uruguay. Agrociencia. 14: 109-113.
CHATTOPADHYAY, P. 2000. Fish - catching and handling. In: ROBINSON, R.K. (ed.): Encyclopedia of Food Microbiology. Academic Press. 1547p.
CHENG, A.C. and CURRIE, B.J. 2005. Melioidosis: epidemiology, pathophysiology and management. Clinical Microbiology Reviews. 18: 383-416.
COSTA, M.M.; PEIXOTO, R.M.; BOIJINK, C.L.; CASTAGNA, L.; MEURER, F.; VARGAS, A.C. 2008. Sensibilidade antimicrobiana de bactérias isoladas de jundiá (Rhamdia quelen). Pesquisa Veterinária Brasileira. 28: 477-480.
CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Secondy Informational Supplement. 15 ed. Document M100-S22, 2012.
FOTI, M.; GIACOPELLO, C.; BOTTARI, T.; FISICHELLA, V.; RINALDO, D.; MAMMINA, C. 2009. Antibiotic Resistance of Gram Negatives isolates from loggerhead sea turtles (Caretta caretta) in the central Mediterranean Sea. Marine Pollut Bulletin.58: 1363-1366.
GUZMÁN, M.C.; BISTONI, M.D.L.A.; TAMAGNINI, L.M.; GONZÁLES, R.D. 2004. Recovery of Escherichia coli in fresh water fish, Jenynsia multidentata and Bryconamericus iheringi. Journal of Great Lakes Reaserch. 38:2368-2374.
HAENEN, O.L.M.; EVANS, J.J.; BERTHE, F. 2013. Bacterial infections from aquatic species: potential for and prevention of contact zoonoses. Scientific and Technical Review of the Office International des Epizooties. 32: 497-507.
HEUER, O.E.; KRUSE, H.; GRAVE, K.; COLLIGNON, P.; KARUNASAGAR, I.; ÂNGULO, F.J. 2009. Human Health Consequences of Use of Antimicrobial Agents in Aquaculture. Clinical and Infectious Disease. 49: 1248-1253.
JANDA, J.M. and ABBOTT, S.L. 2010. The genus Aeromonas: Taxonomy, pathogenicity, and infection. Clinical Microbiology Reviews. 23: 35-73.
KRUMPERMAN, P.H. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high í risk sources of fecal contamination of foods. Applied and Environmental Microbiology. 46: 165-170.
LEHANE, L. and OLLEY, J. 2000. Histamine fish poisoning revisited. International Journal of Food Microbiology. 58: 1-37.
LI, P. e GATLIN,D.M. 2005. Evaluation of the prebiotic GroBiotic®-A and brewers yeast as dietary supplements for sub-adult hybrid striped bass (Morone chrysops x M. saxatilis) challenged in situ with Mycobacterium marinum.Aquaculture. 248: 197-205.
LIU, Y.J. and LU, C.P. 2004. Role ofIchthyophthirius multifiliis in the Infection of Aeromonas hydrophila. Journal of Veterinary Medicine, Series B.51:222-224.
OROZOVA, P.; CHIKOVA, V.; NAJDENSKI, H. 2010. Antibiotic resistance of pathogenic for fish isolates of Aeromonas spp. Bulgarian Journal of Agricultural Science. 16: 376-386.
PAVANELLI, G.C.; EIRAS, J.C.; TAKEMOTO, R.M. 1998. Doenças de peixes: Profilaxia, diagnóstico e tratamento. 3.ed. EDUEM. 264p.
PATHAK, S.P. and GOPAL, K. 2005. Occurrence of antibiotic and metal resistance in bactérias from organs of river fish. Environmental Research. 98: 100-103.
PROCÓPIO, R.C.O. 2000. Ocorrência de bactérias formadoras de histamina em tunídeos utilizados para enlatamento. Niterói, Brasil, Rio de Janeiro. 91f. (Tese de doutorado, Universidade Federal Fluminense). Disponível em:< http://www.ndc.uff.br/content/biblioteca-digital-de-teses-e-disserta%C3%A7%C3%B5es>. Acesso em: 18 abril 2014.
SHAMA, S.; BRANDÃO, D.A.; VARGAS, A.C.; COSTA, M.M.; PEDROZO, A.F. 2000, Bactérias com potencial patogênico nos rins e lesões externas de jundiás (Rhamdia quelen) cultivados em sistema semi-intensivo. Ciência Rural. 10: 293-298.
SPRAGUE, L.D and NEUBAUER, H. 2004. Melioidosis in animals: a review on epizootiology, diagnosis and clinical presentation. Journal of Veterinary Medicine, Series B. 51:305-320.
WEINSTEIN, M.; LOW, D.; MCGEER, A.; WILLEY, B.; ROSE, D.; COULTER, M.; WYPER ,P.; BORCZYK, A.; LOVGREN, M.; FACKLAM, R. 1996. Invasive infection due to Streptococcus iniae: a new or previously unrecognized disease - Ontario.Canada Communicable Disease Report. 22:129-131.
WOLF, J.C. and SMITH, S.A. 1999. Comparative severity of experimentally induced mycobacteriosis in striped bass Morone saxatilis and hybrid tilapia Oreochromis spp. Diseases of Aquatic Organisms. 38: 191-200.
XU, D.H.; PRIDGEON, J.W.; KLESIUS, P.H.; SHOEMAKER, C.A. 2012. Parasitism by protozoan Ichthyophthirius multifiliis enhanced invasion of Aeromonas hydrophila in tissues of channel catfish. Veterinary Parasitology. 184:101-107.
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2016-03-30
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