Trypan blue staining is not efficient in determining oocyte viability in Colossoma macropomum and Brycon amazonicus
DOI:
https://doi.org/10.20950/1678-2305.2017.121.129Keywords:
fish gametes, vital stain, membrane integrity, Amazonian fishAbstract
Currently, there is no effective technique to evaluate the quality of oocytes in fish farming in a practical and affordable way. The cell membrane integrity test with the vital dye trypan blue (TB) could be an option. In this study, Colossoma macropomum and Brycon amazonicus oocytes were exposed to different TB concentrations seeking to verify a possible relationship between the results of staining tests and reproductive rates. Oocytes were exposed to concentrations of 0.05, 0.04, 0.03, 0.02 and 0.01% TB for 1 minute and subsequently were evaluated under a stereomicroscope. The percentage of unstained (viable) oocytes from each sample was correlated with fertilization and hatching rates using a linear regression (P>0.05). We observed a weak correlation between the results of the staining tests and the fertilization and hatching rates in both species. TB integrity tests were not effective in predicting spawning viability in C. macropomum and B. amazonicus.
References
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RAMIREZ, M.E.V.; Le PENNEC, M.; DORANGE, G.; De VAUCHELLE, N.; NONNOTTE, G. 1999 Assessment of female gamete quality in the pacific oyster Crassostrea gigas. Invertebrate
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RIZZO, E.; SATO, Y.; BARRETO, B.P.; GODINHO, H.P. 2002 Adhesiveness and surface patterns of
eggs in Neotropical freshwater teleosts. Journal of Fish Biology, 61(3): 615-632.
RODRIGUES, E.B.; COSTA, E.F.; PENHA F.M.; MELO, G.B.; BOTTÓS, J.; DIB, E., FURLANI, B.; LIMA,
V.C.; MAIA, M.; MEYER, C.H.; HOFLING-LIMA, A. LUISA.; MICHEL. E.F. 2009 The use of vital
dyes in ocular surgery. Survey of Ophthalmology, 54(5): 576-617.
ROMAGOSA, E.; BORELLA, M.I.; NARAHARA, M.Y.; FENERICH-VERANI, N. 2002 Light and electron microscopy oogenesis in matrinxã, Brycon cephalus. Journal of Submicroscopic Cytology and Pathology, 34(4): 425-431.
STREIT JR, D.P.; MORAES, G.V.; RIBEIRO, R.P.R. 2003 Estudo comparativo da indução hormonal
da espermiação em piauvuçu (Leporinus macrocephalus) com extrato de hipófise de frango,
coelho e carpa. Acta Scientiarum Animal Sciences, 25(2): 261-266.
STROBER, W. 1997 Monitoring Growth. Current protocols in immunology, 21: 3A: A.3 A.1í A.3 A.2.RODRIGUES, E.B.; COSTA, E.F.; PENHA F.M.; MELO, G.B.; BOTTÓS, J.; DIB, E., FURLANI, B.; LIMA,
V.C.; MAIA, M.; MEYER, C.H.; HOFLING-LIMA, A. LUISA.; MICHEL. E.F. 2009 The use of vital dyes in ocular surgery. Survey of Ophthalmology, 54(5): 576-617.
ROMAGOSA, E.; BORELLA, M.I.; NARAHARA, M.Y.; FENERICH-VERANI, N. 2002 Light and electron microscopy oogenesis in matrinxã, Brycon cephalus. Journal of Submicroscopic Cytology and Pathology, 34(4): 425-431.
STREIT JR, D.P.; MORAES, G.V.; RIBEIRO, R.P.R. 2003 Estudo comparativo da indução hormonal
da espermiação em piauvuçu (Leporinus macrocephalus) com extrato de hipófise de frango,
coelho e carpa. Acta Scientiarum Animal Sciences, 25(2): 261-266.
STROBER, W. 1997 Monitoring Growth. Current protocols in immunology, 21: 3A: A.3 A.1í A.3 A.2.
TRAN, S.L.; PUHAR, A.; CAMUS, M.N.; RAMARAO, N. 2011 Trypan blue dye enters viable cells
incubated with the pore-forming toxin HlyII of Bacillus cereus. Plos ONE, 6(9): e22876. [on line]
doi: 10.1371/journal. pone.0022876.
VAZZOLER, A.E.A.M. 1996 Biologia da reprodução de peixes teleósteos: teoria e prática. EDUEM. Maringá. 169p.
VIVEIROS, A.T.M.; ORFÃO, L.H.; MARIA, A.N.; ALAMMAN, I.B. 2009 A simple, inexpensive and successful freezing method for curimba Prochilodus lineatus (Characiformes) semen. Animal Reproduction Science, 112(3-4): 293-300.
ZAMPOLLA, T.; ZHANG, T.; RAWSON, D.M. 2008 Evaluation of zebrafish (Danio rerio) ovarian follicle viability by simultaneous staining with fluorescein diacetate and propidium iodide.CryoLetters, 29(6): 463-475.
BOBE, J.; LABBÉ, C. 2010 Egg and sperm quality in fish. General and Comparative Endocrinology,
165(2010): 535-548.
GARCIA, R.F.R.; STREIT Jr, D.P.; CABRITA, E.; GODOY, L.C. 2016 Semen cryopreservation in Brazilian freshwater fish: Advances and main challenges. In: MARCO-JIMÉNEZ, F.; AKDEMIR, H. Cryopreservation in eukaryotes. InTech, Rijeka. p.55-73.
FINN, R.N.; CERDí€, J. 2011 Aquaporin evolution in fishes. Frontiers in Physiology, 2(44): 1-6.
FORNARI, D.C.; RIBEIRO, R.P.; STREIT JR, D.P.; GODOY, L.C.; NEVES, P.R.; OLIVEIRA, D.; SIROL, R.N. 2014 Effect of cryoprotectants on the survival of cascudo preto (Rhinelepis aspera) embryos stored at -8°C. Zygote, 22(1): 58-63.
GUERREIRO, L.R.J.; STREIT JR, D.P.; ROTTA, M.A. 2015 Rastreabilidade na gestão de plantéis de
reprodutores de peixes reofílicos: estudo de caso. Custos e @gronegócio, 11(1): 35-52.
HONORATO-SAMPAIO, K.; PRADO, P.S.; SATO, Y.; BAZZOLI, N.; RIZZO, E. 2015 Comparative morphology of the oocyte surface and early development in four Characiformes from the São Francisco River, Brazil. Journal of Morphology, 276(10): 1258-1272.
IBGE - INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA. 2016 Produção da Pecuária
Municipal 2015. Rio de Janeiro, 43: 1-49. Available at: <http://biblioteca.ibge.gov.br/visualizacao/periodicos/84/ppm_2015_v43_br. pdf>. Access on: 17 Jul. 2017.
ISAíÅ¡, Z.A.; RIZZO, E.; AMARAL, T.B.; MOURAD, N.M.N.; VIVEIROS, A.T.M. 2013 Structural analysis of oocytes, post-fertilization events and embryonic development of the Brazilian endangered teleost Brycon insignis (Characiformes). Zygote, 21(1): 85-94.
KUNZ, Y.W. 2004 Developmental Biology of Teleost fishes. Springer. Dublin. 645p.
LAHNSTEINER, F.; SOARES, F.; RIBEIRO, L.; DINIS, M. T. 2009 Egg quality determination in teleost fish. In: CABRITA, E.; ROBLES, V.; HERRÁEZ, P. Methods in reproductive aquaculture:marine and freshwater species. CRC Press, Taylor &Francis Group, New York, p.149-172.
LEITE, L.V.; MELO, M.A.P.; OLIVEIRA, F.C.E.;PINHEIRO, J.P.S.; CAMPELLO, C.C.; NUNES, J.F.; VANDERLEY, C.S.B. 2013 Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum). Arquivo Brasileiro de Medicina Veterinária e
Zootecnia, 65(2): 421-429.
MELO, R.M.C.; ARANTES, F.P.; SATO, Y.; DOS SANTOS, J.E.; RIZZO, E.; BAZZOLI, N. 2011 Comparative morphology of gonadal structure related to reproductive strategies in six species of neotropical catfishes (teleostei: siluriformes). Journal of Morphology, 272(5): 525-535.
MONTGOMERY, D.C.; PECK, E.A.; VINNING, G.G. 2012 Introduction to linear regression analysis, 5th ed. Wiley. New Jersey. 672p.
MONTOYA, L.N.F.; MARTINS, T.P.; GIMBO, R.Y.; ZANUZZO, F.S.; URBINATI, E.C. 2017 βGlucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus). Fish & Shellfish Immunology, 60: 197-204.
OLIVEIRA, R.G.S. 2015 Indução í espermiação e determinação da dose inseminante de Brycon amazonicus (Spix & Agassiz, 1829) (TELEOSTEI: CHARACIDAE). Manaus. 92p. (Dissertação de
Mestrado. Universidade Nilton Lins - UNL/INPA). Available at: <http://pgaquicultura.inpa.gov.
br/images/Rosilane%20Oliveira.pdf> Access on: 19 Jan. 2016.
OMNES, M.H.; DORANGE, G.; SUQUET, M.; NORMANT, Y. 1999 Application of staining techniques to improve the viability assessment of turbot (Psetta maxima) ova. In: INTERNATIONAL SYMPOSIUM ON THE REPRODUCTIVE PHYSIOLOGY OF FISH, 6th., Bergen, 4-9/July/1999. Proceedings… p.435.
RAMIREZ, M.E.V.; Le PENNEC, M.; DORANGE, G.; De VAUCHELLE, N.; NONNOTTE, G. 1999 Assessment of female gamete quality in the pacific oyster Crassostrea gigas. Invertebrate
Reproduction and Development, 36(1-3): 73-78.
RIZZO, E.; SATO, Y.; BARRETO, B.P.; GODINHO, H.P. 2002 Adhesiveness and surface patterns of
eggs in Neotropical freshwater teleosts. Journal of Fish Biology, 61(3): 615-632.
RODRIGUES, E.B.; COSTA, E.F.; PENHA F.M.; MELO, G.B.; BOTTÓS, J.; DIB, E., FURLANI, B.; LIMA,
V.C.; MAIA, M.; MEYER, C.H.; HOFLING-LIMA, A. LUISA.; MICHEL. E.F. 2009 The use of vital
dyes in ocular surgery. Survey of Ophthalmology, 54(5): 576-617.
ROMAGOSA, E.; BORELLA, M.I.; NARAHARA, M.Y.; FENERICH-VERANI, N. 2002 Light and electron microscopy oogenesis in matrinxã, Brycon cephalus. Journal of Submicroscopic Cytology and Pathology, 34(4): 425-431.
STREIT JR, D.P.; MORAES, G.V.; RIBEIRO, R.P.R. 2003 Estudo comparativo da indução hormonal
da espermiação em piauvuçu (Leporinus macrocephalus) com extrato de hipófise de frango,
coelho e carpa. Acta Scientiarum Animal Sciences, 25(2): 261-266.
STROBER, W. 1997 Monitoring Growth. Current protocols in immunology, 21: 3A: A.3 A.1í A.3 A.2.RODRIGUES, E.B.; COSTA, E.F.; PENHA F.M.; MELO, G.B.; BOTTÓS, J.; DIB, E., FURLANI, B.; LIMA,
V.C.; MAIA, M.; MEYER, C.H.; HOFLING-LIMA, A. LUISA.; MICHEL. E.F. 2009 The use of vital dyes in ocular surgery. Survey of Ophthalmology, 54(5): 576-617.
ROMAGOSA, E.; BORELLA, M.I.; NARAHARA, M.Y.; FENERICH-VERANI, N. 2002 Light and electron microscopy oogenesis in matrinxã, Brycon cephalus. Journal of Submicroscopic Cytology and Pathology, 34(4): 425-431.
STREIT JR, D.P.; MORAES, G.V.; RIBEIRO, R.P.R. 2003 Estudo comparativo da indução hormonal
da espermiação em piauvuçu (Leporinus macrocephalus) com extrato de hipófise de frango,
coelho e carpa. Acta Scientiarum Animal Sciences, 25(2): 261-266.
STROBER, W. 1997 Monitoring Growth. Current protocols in immunology, 21: 3A: A.3 A.1í A.3 A.2.
TRAN, S.L.; PUHAR, A.; CAMUS, M.N.; RAMARAO, N. 2011 Trypan blue dye enters viable cells
incubated with the pore-forming toxin HlyII of Bacillus cereus. Plos ONE, 6(9): e22876. [on line]
doi: 10.1371/journal. pone.0022876.
VAZZOLER, A.E.A.M. 1996 Biologia da reprodução de peixes teleósteos: teoria e prática. EDUEM. Maringá. 169p.
VIVEIROS, A.T.M.; ORFÃO, L.H.; MARIA, A.N.; ALAMMAN, I.B. 2009 A simple, inexpensive and successful freezing method for curimba Prochilodus lineatus (Characiformes) semen. Animal Reproduction Science, 112(3-4): 293-300.
ZAMPOLLA, T.; ZHANG, T.; RAWSON, D.M. 2008 Evaluation of zebrafish (Danio rerio) ovarian follicle viability by simultaneous staining with fluorescein diacetate and propidium iodide.CryoLetters, 29(6): 463-475.
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