Métodos químico e físico de indução í triploidia em Crassostrea gigas(Thunberg, 1793)
Palavras-chave:
temperatura, 6-dimetilaminopurina, citocalasina-B, ostraResumo
Três métodos de indução í triploidia (3N) foram testados em ostras diploides (2N) (Crassostrea gigas): dois métodos químicos, citocalasina-B (CB) e 6-dimetilaminopurina (6-DMAP), e um método físico, com choque de temperatura. O objetivo foi avaliar a tecnologia de indução í triploidia utilizando a técnica de citometria de fluxo como ferramenta para verificação dos resultados de indução. Os experimentos foram realizados em separado, sendo que a temperatura da água do mar foi mantida em 25°C em todos os tanques. No experimento I foi avaliada a eficácia da indução í triploidia com CB (0,5 mg L-1) e 6-DMAP (390 μmols L-1). No experimento II, foi testada a eficiência da indução í triploidia com CB (0,5 mg L-1) e 6-DMAP (450 μmols L-1). No experimento III, foi avaliada a eficiência da indução í triploidia com CB (0,5 mg L-1) e choque de temperatura (25 - 36°C). Nos três experimentos foram obtidas larvas triploides viáveis. Entretanto, nos experimentos I e II (com métodos químicos), observou-se elevada mortalidade das larvas, especialmente para o tratamento CB. A partir destes resultados, a substituição de CB por outros métodos de indução í triploidia é sugerida, devido ao seu elevado custo e alto nível de toxicidade para os seres humanos e para o meio ambiente
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