Anesthesia in oysters of the genus Crassostrea cultured in Brazil
Keywords:
anesthetics, magnesium chloride, Crassostrea gasar, Crassostrea rhizophorae, Crassostrea gigas, D-larvaeAbstract
This study evaluated: 1) the time required for anesthesia induction and recovery of oysters Crassostrea rhizophorae, Crassostrea gasar and Crassostrea gigas using magnesium chloride (MgCl2); 2) the survival after anesthesia and gonad sampling; and 3) the D-larvae generation after anesthesia. For each species, three groups of 10 animals were kept in 50 g L-1 MgCl2, salinity of 36 and temperature of 22°C. One control group was kept in solution without MgCl2. Every 1 h, anesthetized oysters were recorded, sampled to determine sex and placed in clean seawater to assess recovering every 30 min. Gonad samplings were made beside the posterior adductor muscle, using 1 mL syringes and needles (0.60 x 25 mm). Factorial crosses were generated within and between anesthetized and non-anesthetized oyster groups to produce D-larvae in C. gigas. The survival after 10 days of anesthesia was 100% for the three studied species. For C. rhizophorae and C. gigas, 100% of the animals were anesthetized after 360 min and were recovered after 240 and 150 min respectively. For C. gasar, 87% were anesthetized after 720 min and recovered after >240 min. There were no significant differences in D-larvae numbers between factorial crosses. The salt MgCl2 served as an efficient relaxant and did not cause any deleterious effect on the survival of the three studied species, or on the D-larvae generation in C. gigas.
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