Efeito anestésico e toxicidade aguda do óleo essencial de Citrus sinensis em betta
Palavras-chave:
Comportamento, Limoneno, Laranja, Peixe ornamental, EstresseResumo
Este estudo investigou o uso do óleo essencial de Citrus sinensis (OECS) em Betta splendens, avaliando a toxicidade, tempos de indução e recuperação, sua ação sobre o comportamento agonista e no transporte coletivo de machos. Para a toxicidade, peixes foram expostos ao OECS em diferentes concentrações durante 48 h. O teste de indução foi realizado para avaliar a sedação, anestesia e recuperação sob diferentes concentrações. Para avaliar o comportamento agonista, indivíduos machos foram mantidos com diferentes dosagens de OECS, avaliando batimento opercular e expansão das nadadeiras, enquanto expostos a animais não tratados. No estudo de transporte coletivo de machos, foram divididos em dois tratamentos com dois níveis de OECS, durante 6h, analisando também a histometria branquial ao final do transporte. Os resultados obtidos mostram que a concentração letal média em 48h de exposição ao OECS foi calculada em 49,17μL·L−1. O menor tempo de indução à anestesia foi com 300 μL·L−1 OECS. O OECS foi capaz de reduzir o comportamento agonista em indivíduos machos. As análises histométricas revelaram uma redução na altura dos filamentos branquiais dos peixes transportados com 20 μL·L-1 OECS. O OECS pode ser usado como agente sedativo e anestésico para o B.splendens.
Referências
Adamek-Urbańska, D.; Błazewicz, E.; Sobień, M.; Kasprzak, R.; Kamaszewski, M. 2021. Histological study of suprabranchial chamber membranes in anabantoidei and clariidae fishes. Animals, 11(4): 1158. https://doi.org/10.3390/ani11041158
Almeida, A.P.G.; Heinzmann, B.M.; Val, A.L.; Baldisserotto, B. 2018. Essential oils and eugenol as anesthetics for Serrasalmus rhombeus. Boletim do Instituto de Pesca, 44(1): 44-50. https://doi.org/10.20950/1678-2305.2018.195
Alton, L.A.; Portugal, S.J.; White, C.R. 2013. Balancing the competing requirements of air-breathing and display behaviour during male-male interactions in Siamese fighting fish Betta splendens. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 164(2): 363-367. https://doi.org/10.1016/j.cbpa.2012.11.012
Aydın, B.; Barbas, L.A.L. 2020. Sedative and anesthetic properties of essential oils and their active compounds in fish: a review. Aquaculture, 520: 734999. https://doi.org/10.1016/j.aquaculture.2020.734999
Becker, A.G.; Parodi, T.V.; Heldwein, C.G.; Zeppenfeld, C.C.; Heinzmann, B.M.; Baldisserotto, B. 2012. Transportation of silver catfish, Rhamdia quelen, in water with eugenol and the essential oil of Lippia alba. Fish Physiology and Biochemistry,38: 789-796. https://doi.org/10.1007/s10695-011-9562-4
Becker, A.J.; Fogliarini, C.O.; Souza, C.F.; Becker, A.G.; Mourão, R.H.V.; SILVA, L.V.F.; Baldisserotto, B. 2018.Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: a comparative approach between different essential oils. Revista Brasileira de Zootecnia, 47: e20170185. https://doi.org/10.1590/rbz4720170185
Bolasina, S.N.; Azevedo, A.; Petry, A.C. 2017. Comparative efficacy of benzocaine, tricaine methanesulfonate and eugenol as anesthetic agents in the guppy Poecilia vivipara. Aquaculture Reports, 6: 56-60. https://doi.org/10.1016/j.aqrep.2017.04.002
Brandão, F.R.; Farias, C.F.S.; Souza, D.C.M.; Oliveira, M.I.B.; Matos, L.V.; Majolo, C.; Oliveira, M.R.; Chaves, F.C.M.; O’sullivan, F.L.A.; Chagas, E.C. 2021. Anesthetic potential of the essential oils of Aloysia triphylla, Lippia sidoides and Mentha piperita for Colossoma macropomum. Aquaculture, 534: 736275. https://doi.org/10.1016/j.aquaculture.2020.736275
Can, E.; Kizak, V.; Can, S.S.; Özçiçek, E. 2018. Anesthetic potential of geranium (Pelargonium graveolens) oil for two cichlid species, Sciaenochromis fryeri and Labidochromis caeruleus. Aquaculture, 491: 59-64. https://doi.org/10.1016/j.aquaculture.2018.03.013
Castro, N.; Ros, A.F.H.; Becker, K.; Oliveira, R.F. 2006. Metabolic costs of aggressive behavior in the siamese fighting fish, Betta splendens. Aggressive Behavior, 32(5): 474-480. https://doi.org/10.1002/ab.20147
Craft, B.B.; Velkey II, A.J.; Szalda-Petree, A. 2003. Instrumental conditioning of choice behavior in male Siamese fighting fish (Betta splendens). Behavioural Processes, 63(3): 171-175. https://doi.org/10.1016/S0376-6357(03)00079-2
Eisenreich, B.R.; Szalda-Petree, A. 2015. Behavioral effects of fluoxetine on aggression and associative learning in Siamese fighting fish (Betta splendens). Behavioural Processes, 121: 37-42. https://doi.org/10.1016/j.beproc.2015.10.008
Ferreira, A.L.; Silva, W.S.; Neves, L.C.; Ferreira, N.S.; Takata, R.; Luz, R.K. 2020. Benzocaine and menthol as anesthetics for the African cichlid Aulonocara nyassae. Aquaculture International, 28: 1837-1846. https://doi.org/10.1007/s10499-020-00561-w
França, I.F.; Heluy, G.M.; Schultz, E.B.; Vianna, W.O.; Pereira, M.M.; Ramos, L.R.V. 2021. Sex reversal in siamese fighting fish larvae by thermal management. Boletim do Instituto de Pesca, 47: e676. https://doi.org/10.20950/1678-2305/bip.2021.47.e676
Greene, S.M.; Szalda-Petree, A.D. 2022. Fins of fury or fainéant: fluoxetine impacts the aggressive behavior of fighting fish (Betta splendens). Behavioural Processes, 194: 104544. https://doi.org/10.1016/j.beproc.2021.104544
Hamilton, M.A.; Russo, R.C.; Thurston, R.V. 1977. Trimmed spearman-karber method for estimating median lethal concentrations in toxicity bioassays. Environmental Science & Technology, 11(7): 714-719. https://doi.org/10.1021/es60130a004
Heluy, G.M.; Ramos, L.R.V.; Pedrosa, V.F.; Sarturi, C.; Figueiredo, P.G.P.; Vidal, L.G.P.; França, I.F.; Pereira,M.M. 2020. Oregano (Origanum vulgare) essential oil as an additive in diets for Nile tilapia (Oreochromis niloticus) fingerlings reared in salinized water. Aquaculture Research, 51(8), 3237-3243. https://doi.org/10.1111/are.14658
Kasanen, J.-P.; Pasanen, A.-L.; Pasanen, P.; Liesivuori, J.; Kosma, V.-M; Alarie, Y. 1998. Stereospecificity of the sensory irritation receptor for nonreactive chemicals illustrated by pinene enantiomers. Archives of Toxicology, 72(8): 514-523. https://doi.org/10.1007/s002040050536
Kizak, V.; Can, E.; Danabaş, D.; Can, Ş.S. 2018. Evaluation of anesthetic potential of rosewood (Aniba rosaeodora) oil as a new anesthetic agent for goldfish (Carassius auratus). Aquaculture, 493: 296-301. https://doi.org/10.1016/j.aquaculture.2018.05.013
Koca, Y.B.; Kara, N. 2022. Alterations in gills and intestine of Danio rerio after exposure to acaricide yoksorrun-5EC (hexythiazox): histopathologic and morphometric evaluation. Drug and Chemical Toxicology, 45(4): 1808-1817. https://doi.org/10.1080/01480545.2021.1880428
Komiya, M.; Takeuchi, T.; Harada, E. 2006. Lemon oil vapor causes an anti-stress effect via modulating the 5-HT and DA activities in mice. Behavioural Brain Research, 172(2):240-249. https://doi.org/10.1016/j.bbr.2006.05.006
Lopes, J.M.; Sousa, C.F.; Schindler, B.; Pinheiro, C.G.; Salbego, J.; Siqueira, J.C.; Heinzmann, B.M.; Baldisserotto, B. 2018. Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish (Rhamdia quelen). Neotropical Ichthyology, 16(2): e170152. https://doi.org/10.1590/1982-0224-20170152
Luna, L.G. 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology. New York: McGraw-Hill. 258 p.
Mercier, B.; Prost, J.; Prost, M. 2009. The essential oil of turpentine and its major volatile fraction (alpha- and beta-pinenes): a review. International Journal of Occupational Medicine and Environmental Health, 22(4): 331-342. Retrieved from: https://www.researchgate.net/publication/41669083_The_essential_oil_of_turpentine_and_its_major_volatile_fraction_a-_and_b-pinenes_A_review. Access on 10 oct.23
Mitjana, O.; Bonastre, C.; Insua, D.; Falceto, M.V.; Esteban, J.; Josa, A.; Espinosa, E. 2014. The efficacy and effect of repeated exposure to 2-phenoxyethanol, clove oil and tricaine methanesulphonate as anesthetic agents on juvenile Angelfish (Pterophyllum scalare). Aquaculture, 433: 491-495. https://doi.org/10.1016/j.aquaculture.2014.07.013
Monticini, P. 2010. The ornamental fish trade. Production and commerce of ornamental fish: technical-managerial and legislative aspects.Rome: Food and Agriculture Organization of the United Nations. 134 p.
Oliveira, I.C.; Oliveira, R.S.M.; Lemos, C.H.P.; Oliveira, C.P.B.; Felix e Silva, A.; Lorenzo, V.P.; Lima, A.O.; Cruz, A.L.; Copatti, C.E. 2022. Essential oils from Cymbopogon citratus and Lippia sidoides in the anesthetic induction and transport of ornamental fish Pterophyllum scalare. Fish Physiology and Biochemistry, 48: 501-519. https://doi.org/10.1007/s10695-022-01075-3
Park, H.M.; Lee, J.H.; Yaoyao, J.; Jun, H.J.; Lee, S.J. 2011. Limonene, a natural cyclic terpene, is an agonisticligand for adenosine A(2A) receptors. Biochemical and Biophysical Research Communications, 404(1): 345-348.https://doi.org/10.1016/j.bbrc.2010.11.121
Parodi, T.V.; Cunha, M.A.; Becker, A.G.; Zeppenfeld, C.C.; Martins, D.I.; Koakoski, G.; Barcellos, L.G.; Heinzmann, B.M.; Baldisserotto, B. 2014. Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen. Fish Physiology and Biochemistry, 40: 323-334. https://doi.org/10.1007/s10695-013-9845-z
Pattanasiri, T.; Taparhudee, W.; Suppakul, P. 2017a. Acute toxicity and anesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens. Aquaculture International, 25: 163-175. https://doi.org/10.1007/s10499-016-0020-2
Pattanasiri, T.; Taparhudee, W.; Suppakul, P. 2017b. Anaesthetic efficacy of clove oil-coated LDPE bag on improving water quality and survival in the Siamese fighting fish, Betta splendens, during transportation. Aquaculture International, 25: 197-209. https://doi.org/10.1007/s10499-016-0022-0
Pedrazzani, A.S.; Ostrensky Neto, A. 2016. The anaesthetic effect of camphor (Cinnamomum camphora), clove (Syzygium aromaticum) and mint (Mentha arvensis) essential oils on clown anemonefish, Amphiprion ocellaris (Cuvier 1830). Aquaculture Research, 47(3), 769-776. https://doi.org/10.1111/are.12535
Pirhonen, J.; Schreck, C.B. 2003. Effects of anesthesia with MS-222, clove oil and CO2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture, 220(1-4): 507-514. https://doi.org/10.1016/S0044-8486(02)00624-5
Raspo, M.A.; Vignola, M.B.; Andreatta, A.E.; Juliani, H.R. 2020. Antioxidant and antimicrobial activity of citrus essential oils from Argentina and the United States. Food Bioscience, 36: 100651. https://doi.org/10.1016/j.fbio.2020.100651
Ross, L.G.; Ross, B. 2008. Anesthetic and sedative techniques for aquatic animals. Oxford: Blackwell Publishing, 228 p. https://doi.org/10.1002/9781444302264
Salbego, J.; Toni, C.; Becker, A.G.; Zeppenfeld, C.C.; Menezes, C.C.; Loro, V.L.; Heinzmann, B.M.; Baldisserotto, B. 2017. Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water. Brazilian Journal of Biology, 77(4):696-702. https://doi.org/10.1590/1519-6984.16515
Santos, E.L.R.; Rezende, F.P.; Moron, S.E. 2020. Stressrelated physiological and histological responses oftambaqui (Colossoma macropomum) to transportation in water with tea tree and clove essential oil anesthetics. Aquaculture, 523: 735164. https://doi.org/10.1016/j.aquaculture.2020.735164
SAS Institute. 2008. SAS/STAT 9.2. Users guide. Cary: SAS Institute Inc.
Silva, L.L.; Garlet, Q.I.; Benovit, S.C.; Dolci, G.; Mallmann, C.A.; Bürger, M.E.; Baldisserotto, B.; Longhi, S.J.; Heinzmann, B.M. 2013. Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen). Brazilian Journal of Medical and Biological Research, 46(9): 771-779. https://doi.org/10.1590/1414-431X20133013
Silva, L.A.; Martins, M.A.; Santo, F.E.; Oliveira, F.C.; Chaves, F.C.M.; Chagas, E.C.; Martins, M.L.; Campos, C.M. 2020. Essential oils of Ocimum gratissimum and Zingiber officinale as anesthetics for the South American catfish Pseudoplatystoma reticulatum. Aquaculture, 528: 735595. https://doi.org/10.1016/j.aquaculture.2020.735595
Small, B.C. 2003. Anesthetic efficacy of metomidate and comparison of plasma cortisol responses totricainemethanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus.Aquaculture, 218(1-4): 177-185. https://doi.org/10.1016/S0044-8486(02)00302-2
Souza, A.S.L.; Peret, A.C.; Hamoy, M.; Souza, R.A.L.; Torres, M.F.; Barbas, L.A.L. 2019. Propofol and essential oil of Nepeta cataria induce anaesthesia and marked myorelaxation intambaqui Colossoma macropomum: Implications on cardiorespiratory responses. Aquaculture, 500: 160-169. https://doi.org/10.1016/j.aquaculture.2018.10.017
Summerfelt, R.C.; Smith, L.S. 1990. Anaesthesia, surgery, and related techniques. In: Shreck C.B.; Moyle P.B. (eds.). Methods for Biology. Bethesda: America Fisheries Society. pag. 213-272.
Teixeira, R.R.; Souza, R.C.; Sena, A.C.; Baldisserotto, B.; Heinzmann, B.M.; Copatti, C.E. 2018. Essential oil of Aloysia triphylla is effective in nile tilapia transport. Boletim do Instituto de Pesca, 44(1): 17-24. https://doi.org/10.20950/1678-2305.2018.263
Teixeira, R.R.; De Souza, R.C.; Sena, A.C.; Baldisserotto, B.; Heinzmann, B.M.; Couto, R.D.; Copatti, C.E. 2017. Essential oil of Aloysia triphylla in Nile tilapia: anaesthesia, stress parameters and sensory evaluation of fillets. Aquaculture Research, 48(7): 3383-3392. https://doi.org/10.1111/are.13165
Vanderzwalmen, M.; Edmonds, E.; Carey, P.; Snellgrove, D.; Sloman, K.A. 2020. Effect of a water conditioner on ornamental fish behavior during commercial transport. Aquaculture, 514: 734486. https://doi.org/10.1016/j.aquaculture.2019.734486
Ventura, A.S.; Jerônimo, G.T.; Filho, R.A.C.C.; Souza, A.I.; Stringhetta, G.R.; Cruz, M.G.; Torres, G.S.; Gonçalves, L.U.; Povh, J.A. 2021. Ocimum basilicum essential oil as an anesthetic for tambaqui Colossoma macropomum: Hematological, biochemical, non-specific immune parameters and energy metabolismo. Aquaculture, 533:736124. https://doi.org/10.1016/j.aquaculture.2020.736124
Vu, T.-D.; Iwasaki, Y.; Oshima, K.; Chiu, M.-T.; Nikaido, M.; Okada, N. 2021. A unique neurogenomic state emerges after aggressive confrontations in males of the fish Betta splendens. Gene, 784:145601. https://doi.org/10.1016/j.gene.2021.145601
Wang, J.; Xiong, G.; Bai, C.; Liao, T. 2021. Anesthetic efficacy of two plant phenolics and the physiological response of juvenile Ictalurus punctatus to simulated transport. Aquaculture, 538: 736566. https://doi.org/10.1016/j.aquaculture.2021.736566
Waristha, A.; Kingkaew, W.; Kumthorn, T. 2011. Acute toxicity of clove oil and effects on histopathological changes in gill of Siamese fighting fish Betta Splendens. Research Journal of Chemistry and Environment, 15(2): 139-146.
Zhang, W.; Wang, H.; Brandt, D.Y.C.; Hu, B.; Sheng, J.; Wang, M.; Luo, H.; Guo, S.; Sheng, B.; Zeng, Q.; Peng, K.; Zhao, D.; Jian, S.; Wu, D.; Wang, J.; van Esch, J.H.M.; Shi, W.; Ren, J.; Nielsen, R.; Hong, Y. 2022. The genetic architecture of phenotypic diversity in the betta fish (Betta splendens). Science advances, 8(38): eabm4955. https://doi.org/10.1126/sciadv.abm4955
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Copyright (c) 2023 Rafael Carvalho da Silva, Louis Ramos Silva, Igor de Fiuza França, Jane Mello Lopes, Bruna Tassia dos Santos Pantoja, Marcelo Maia Pereira, Leonardo Rocha Vidal Ramos
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