Use of exogenous enzymes in diets for juvenile pompano trachinotus marginatus: growth and liver and intestine morphophysiology


  • Cleber dos Santos SIMIÃO Universidade Federal do Rio Grande -  FURG, Programa de Pós-graduação em Aquicultura
  • Leonardo Rocha Vidal RAMOS Universidade Federal Rural do Rio de Janeiro -  UFRRJ, Instituto de Zootecnia, Departamento de Produção Animal
  • Agnes MORI Adisseo Brasil Nutrição Animal
  • Luis Alberto ROMANO Universidade Federal do Rio Grande -  FURG, Instituto de Oceanografia, Laboratório de Patologia e Imunologia de Organismos Aquáticos
  • José Maria MONSERRAT Universidade Federal do Rio Grande -  FURG, Instituto de Ciências Biológicas, Laboratório de Bioquí­­mica Funcional de Organismos Aquáticos
  • Marcelo Borges TESSER Universidade Federal do Rio Grande -  FURG, Instituto de Oceanografia, Laboratório de Nutrição de Organismos Aquáticos



carbohydrase, growth, phytase, proteinase


This study investigated the use of exogenous enzymes in diets of juvenile pompano Trachinotus marginatus. Six diets (43% protein, 8% lipid), containing 0 (TC), 25 (T25), 50 (T50), 100 (T100), 250 (T250), and 500 (T500) mg kg-1 of multiple enzymes Rovabio ® MAX AP were produced. The effects on growth performance, body composition, liver glycogen, triglyceride levels, and intestinal and hepatic morphological alterations were assessed. The results showed significant differences (p < 0.05) in growth performance among fish fed diets T100, T250, and T500, with high liver triglyceride accumulation and changes in morphology in the fish fed diets T250 and T500. The calcium concentration in the bones showed a significant difference (P < 0.05) only in fish fed diets T50, T100, and T250 in relation to control. However, there were no significant differences in body composition, intestinal morphology, or liver glycogen concentration. These results suggest that 100 mg enzyme per kg diet was the optimal level of inclusion for juvenile pompano, favoring improved growth performance and calcium concentration in the bones without significant effects on liver triglyceride levels


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