Diversidade genética de populações naturais e estoques de reprodutores de curimba para programas de repovoamento dos Rios Tietê, Grande, Pardo e Mogi-Guaçu (Brasil)

Autores

  • Nelson Mauricio LOPERA-BARRERO Departamento de Zootecnia, Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Londrina (UEL) http://orcid.org/0000-0002-7154-5134
  • Silvio Carlos Alves Dos SANTOS Hidrelétrica AES Tietê -  Promissão
  • Maria Del Pilar RODRIGUEZ-RODRIGUEZ Departamento de Zootecnia, Universidade Federal dos Vales de Jequetinhonha e Mucuri (UFVJM) http://orcid.org/0000-0003-2124-6336
  • Darci Carlos FORNARI Genetic Fish Rise - Primavera do Norte
  • Camila ZANCHETA Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá (UEM)
  • Angela Rocio POVEDA-PARRA Departamento de Zootecnia, Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Londrina (UEL) http://orcid.org/0000-0002-7224-4747
  • Carlos Antonio Lopes de OLIVEIRA Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá (UEM)
  • Ricardo Pereira RIBEIRO Departamento de Zootecnia, Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá (UEM)

Palavras-chave:

Prochilodus lineatus, conservação genética, diferenciação genética, microssatélites

Resumo

Prochilodus lineatus é um peixe tropical que nos últimos anos tem apresentado redução do número de populações selvagens. Programas de repovoamento foram desenvolvidos como método de conservação, e o monitoramento genético das populações e reprodutores é importante para assegurar a viabilidade de tais programas. O objetivo do estudo foi avaliar a diversidade genética de amostras selvagens (WSamp) e de reprodutores de P. lineatus do programa de repovoamento dos rios Tietê, Grande, Pardo e Mogi-Guaçu (São Paulo - Brasil), utilizando marcadores microssatélites. Alta variabilidade genética intrapopulacional foi observada. O número de alelos por loco variou de três a sete e foi detectada a diferenciação dos alelos entre WSamp e os reprodutores. Essa diferenciação foi confirmada por meio da análise do dendrograma. Os valores positivos da FIS indicaram a presença de endogamia em sete das 10 WSamp. A AMOVA e o FST indicaram pequena e moderada diferenciação genética entre as WSamp e alta diferenciação genética em comparação com os reprodutores. Esta diferenciação foi confirmada pelos valores de distí­¢ncia e identidade genética e pelo número de indiví­­duos migrantes. Os resultados indicaram que houve alta variabilidade genética intrapopulacional, similaridade genética em WSamp e reprodutores e diferenciação entre WSamp e reprodutores. Pretende-se que este trabalho possa ser usado para auxiliar os programas de repovoamento de P. lineatus na região estudada e sirva como modelo de monitoramento para outros programas realizados no Brasil.

Referências

ABDUL-MUNEER, P.M. 2014 Application of microsatellite markers in conservation genetics
and fisheries Management: recent advances in population structure analysis and conservation
strategies. Genetics Research International, 1: 1-14.

AGOSTINHO, A.A.; VAZZOLER, A.E.A.M.;GOMES, L.C.; OKADA, E.K. 1993 Spatial distribution and behaviour of Prochilodus scrofa during its life cycle, in the high Parana river floodplain and Itaipu reservoir, Parana, Brazil.Revue d’Hydrobiologie Tropicale, 26(1): 79-90.

AGOSTINHO, A.A.; THOMAZ, S.M.; GOMES, L.C.2005 Conservation of the biodiversity of Brazil’s
inland waters. Conservation Biology, 19(3): 646-652.

AGOSTINHO, A.A.; PELICICE, F.M.; GOMES, L.C.2008 Dams and the fish fauna of the Neotropical
region: impacts and management related to diversity and fisheries. Brazilian Journal of
Biology, 68(4): 1119-1132.

AGOSTINHO, A.A.; AGOSTINHO, C.S.; PELICICE,F.M.; MARQUES, E.E. 2012 Fish ladders: safe
fish passage or hotspot for predation? Neotropical Ichthyology, 10(4): 687-696.

AGUIAR, J.; SCHNEIDER, H.; GOMES, F.;CARNEIRO, J.; SANTOS, S.; RODRIGUES, L.R.;SAMPAIO, I. 2013 Genetic variation in nativeband farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems. Anais da Academia Brasileira de Ciências, 85(4): 1439-1447.

ALLENDORF, F.W. and PHELPS, S.R. 1980 Loss of genetic variation in a hatchery stock of cutthroat
trout. Transactions of the American Fisheries Society, 109(5): 537í 543.

ALMEIDA, F.S.; SODRÉ, L.M.K.; CONTEL, E.P.B.2003 Population structure analysis of Pimelodus
maculatus (Pisces, Siluriformes) from the Tietê and Paranapanema Rivers (Brazil). Genetics and
Molecular Biology, 26(3): 301-305.

ARAKI, H. and SCHMIT, C. 2010 Is hatchery stocking a help or harm? Evidence, limitations
and future directions in ecological and genetic surveys. Aquaculture, 308(1): 2-11.

AUNG, O.; NGUYEN, T.T.T.; POOMPUANG, S.;KAMONRAT, W. 2010 Microsatellite DNA markers revealed genetic population structure among captive stocks and wild populations of mrigal, Cirrhinus cirrhosus in Myanmar. Aquaculture, 299(1-4): 37-43.

BARROCA, T.M.; SANTOS, G.B.; DUARTE, N.V.R.;KALAPOTHAKIS, E. 2012 Evaluation of genetic
diversity and population structure in a commercially important freshwater fish Prochilodus costatus (Characiformes,Prochilodontidae) using complex hypervariable repeats. Genetics and Molecular Research, 11(4):4456-4467.

BASSAM, B.J.; CAETANO-ANOLLÉS, G.;GRESSHOFF, P.M. 1991 Fast and sensitive silver staining of DNA in polyacrylamide gels.Analytical Biochemistry, 196(1): 80-83.

BLANCO GONZALEZ, E.; MURAKAMI, T.;YUNEJI, T.; NAGASAWA, K.; UMINO, T. 2009 Reduction in size-at-age of black sea bream (Acanthopagrus schlegelii) following intensive releases of cultured juveniles in Hiroshima Bay,Japan. Fisheries Research, 99(2): 130í 133.

BORREL, Y.J.; CARLEOS, C,E,; ASTURIANO, J.F.; BERNARDO, D.; VÁZQUEZ, E.; CORRAL, N.;SÁNCHEZ, J.A.; BLANCO, G. 2007 Use of microsatellites and a combinatorial optimization approach in the acquisition of gilthead seabream (Sparus aurata L.) broodstocks for hatcheries.Aquaculture, 269(1-4): 200-210.

BRADIC, M.; BEERLI, P.; GARCÍA-DE LEÓN, F. J.;ESQUIVEL-BOBADILLA, S.; BOROWSKY, R. L. 2012. Gene flow and population structure in the Mexican blind cavefish complex (Astyanax mexicanus). BMC Evolutionary Biology, 12(9): 2-16.

BUCKUP, P.; MENEZES, N.; GHAZZI, M. 2007 Book of freshwater fish species in Brazil. Rio de Janeiro:Museu Nacional. 195p.

CABALLERO, A.; RODRÍGUEZ-RAMILO, S.T.;ÁVILA, V.; FERNÁNDEZ, J. 2010 Management of genetic diversity of subdivided populations in conservation programmes. Conservation Genetics,11(2): 409-419.

CAMP, 1998 Report of the workshop Conservation Assessment and Management Plan (CAMP) for
freshwater fishes of India. Coimbatore: Zoo Outreach Organization (ZOO) and National Bureau of Fish Genetic Resources (NBFGR). 137p.

CARVALHO-COSTA, L.F.; HATANAKA, T.; GALETTI JR, P.M. 2008 Evidence of lack of population substructuring in the Brazilian freshwater fish Prochilodus costatus. Genetics and Molecular Biology, 31(1): 377-380.

CORNUET, J.M.; and LUIKART, G. 1996 Description and power analysis of two tests for detecting
recent population bottlenecks from allele frequency data. Genetics, 144(4): 2001-2014.

DEHAIS, C.; EUDELINE, R.; BERREBI, P.;ARGILLIER, C. 2010 Microgeographic genetic isolation in chub (Cyprinidae: Squalius cephalus)population of the Durance River: estimating fragmentation by dams. Ecology of Freshwater Fish, 19(2): 267-278.

ESGUÍCERO, A.L.H. and ARCIFA, M.S. 2010 Fragmentation of a Neotropical migratory fish
population by a century-old dam. Hydrobiologia,638(1): 41-53.

ESPINACH-ROS, A. and DELFINO, R. 1993 Status of fisheries in the River Plata basin in Paraguay,
Bolí­­via, Argentina and Uruguay. FAO Fisheries Information, 490: 36-51.

EXCOFFIER, L.; LAVAL, G.; SCHNEIDER, S. 2005 Arlequin ver. 3.0: an integrated software
package for population genetics data analysis.Evolutionary Bioinformatics Online, 1: 47-50.

FALCONER, D.S. 1989 Introduction to Quantitative Genetics. 3rd edition. New York: Longman. 438p.

FOPP-BAYAT, D. 2010 Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), cultured in a Polish fish farm.Acta Ichthyologica et Piscatoria, 40(1): 21í 25.

FOPP-BAYAT, D.; JANKUN, M.; KUZMINSKI, H.2010 Genetic characterization of Polish cultured
brook trout, Salvelinus fontinalis (Mitchill), based on microsatellite DNA analysis. Archives of Polish Fisheries, 18(2): 93-99.

GARDNER, M.J.; COTTINGHAM, A.; PHILLIPS,N.M.; HESP, S.A.; CHAPLIN, J.A.; JENKINS, G.I. 2010 Biological performance and genetics of restocked and wild Black Bream in the Blackwood river estuary. Report to the West Australian Fish Foundation. Australia: Project Funded by the Department of Fisheries. 60p.

GARCEZ, R.; CALCAGNOTTO, D.; ALMEIDATOLEDO, L.F. 2011 Population structure of the migratory fish Prochilodus lineatus (Characiformes)from rio Grande basin (Brazil), an area fragmented by dams. Aquatic Conservation, 21(3): 268-275.

GARCÍA DE LEÓN, F.J.; CHIKHI, L.; BONHOMME,F. 1997 Microsatellite polymorphism and
population subdivison in natural populations of Europe sea bass Dicenbrachus labrax (Linnaeus,
1758). Molecular Ecology, 6(1): 51-62.

GODINHO, A.L. e KYNARD, B. 2006 Migration and Spawning of Radio-Tagged Zulega Prochilodus
argenteus in a Dammed Brazilian River.Transactions of the American Fisheries Society,139(3): 1-10.

GODOY, M.P. 1975 Peixes do Brasil. Subordem Characoidei. Bacia do rio Mogi-Guassu. Piracicaba:
Editora Franciscana. p.219-397.

GOLDSTEIN, D.B. and SCHLOTTERER, C. 1999 Microsatellites: Evolution and Applications. Oxford:
Oxford University Press. 368p.

GONZÁLEZ-WANGUEMERT, M.; FERNÁNDEZ,T.V.; PÉREZ-RUZAFA, A.; GIACALONE, M.; D’ANNA, G.; BADALAMENTI, F. 2012 Genetic considerations on the introduction of farmed fish in marine protected areas: The case of study of white seabream restocking in the Gulf of Castellammare (Southern Tyrrhenian Sea).Journal of Sea Research, 68: 41-48.

GOUDET, J. 2002 FSTAT: A Program to Estimate and Test Gene Diversities and Fixation Indices (version 2.9.3.2). Lausanne, R: Department of Ecology and Evolution. Available on:<http//www.unil.ch/izea/softwares/fstat.html>Access at: 13 Mar. 2014.

HALE, M.L.; BURG, T.M.; STEEVES, T.E. 2012 Sampling for microsatellite-based population genetic studies: 25 to 30 individuals per population is enough to accurately estimate allele frequencies. PLoS ONE, 7(9): 1-10.

HARTL, D.L.; CLARK, A.G. 2007 Principles of Population Genetics (Fourth edition). Sunderland
Massachusetts: Sinauer Associates. 565p.

HATANAKA, T.; HENRIQUE-SILVA, F.; GALETTI JR, P.M. 2006 Population substructuring in a migratory freshwater fish Prochilodus argenteus (Characiformes, Prochilodontidae) from the São
Francisco River. Genetica, 126(1-2): 153-159.

HATANAKA, T.; HENRIQUE-SILVA, F.; GALETTI JR, P.M. 2002 A polymorphic, telomeric-like
sequence microsatellite in the Neotropical fish Prochilodus. Cytogenetic and Genome Research,
98(4): 308-310.

LACY, R.C. 1987 Loss of genetic diversity from managed populations: Interacting effects of drift,
mutation, selection, and population subdivision.Conservation Biology, 1(2): 143í 158.

LAROCHE, J. and DURAND, J.D. 2004 Genetic structure of fragmented populations of a
threatened endemic percid of the Rhône river:Zingel asper. Heredity, 92(4): 329-334.

LOPERA BARRERO, N.M. 2009 Conservation of Brycon orbignyanus natural populations and
stocks for their reproductive, genetic, environmental sustainability: A model for species threatened with extinction. Ciencia e Investigación Agrária, 36(2): 191-208.

LOPERA-BARRERO, N.M.; POVH, J.A.; RIBEIRO,R.P.; GOMES, P.C.; JACOMETO, C.B.; LOPES,
T.S. 2008 Comparison of DNA extraction protocols of fish fin and larvae samples:
modified salt (NaCl) extraction. Ciencia e Investigación Agrária, 35(2): 65-74.

LOPERA BARRERO, N.M.; RIBEIRO, R.P.; POVH, J.A.; SIROL, R.N.; MANGOLIN, C.A. 2010a Genetic evaluation of pacu (Piaractus mesopotamicus) natural populations and from the broodstocks of a stock enhancement program using microsatellite markers. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62(4): 954-963.

LOPERA BARRERO, N.M.; RODRIGUEZRODRIGUEZ, M DEL P.; RIBEIRO, R.P.; POVH, J.A.; VARGAS, L.; STREIT JR, D.P.; FORNARI,D.C. 2010b Reproductive contribution of a Brycon orbignyanus (Cuvier and Valenciennes,1850) offspring in the semi-natural reproductive system using microsatellite markers. Zootecnia Tropical, 28(3): 403-411.

MARCENIUK, A.P.; HILSDORF, A.W.S.;LANGEANI, F. 2011 The ichthyofauna from the headwaters of the rio Tietê, São Paulo, Brazil.Biota Neotropica, 11(3): 217-236.

MARIE, A.D.; BERNATCHEZ, L.; GARANT, D. 2010 Loss of genetic integrity correlates with stocking
intensity in brook charr (Salvelinus fontinalis).Molecular Ecology, 19(10): 2025-2037.

MU, X.D.; HU, Y.C.; WANG, X.J.; SONG, H.M.;YANG, Y.X.; LUO, J.R. 2011 Genetic variability in cultured stocks of Scleropages formosus in Mailand China revealed by microsatellite markers. Journal of Animal and Veterinary Advances, 10(5): 555-561.

NA-NAKORN, U. and MOEIKUM, T. 2009 Genetic diversity of domesticated stocks of striped
catfish, Pangasianodon hypophthalmus (Sauvage 1878), in Thailand: relevance to broodstock
management regimes. Aquaculture, 297(1-4): 70-77.

NEI, M. 1978 Estimation of average heterozygosity and genetic distance from a small number of
individuals. Genetics, 89(3): 583-590.

O’CONNELL, M. and WRIGHT, J.M. 1997 Microsatellite DNA in fishes. Reviews in Fish
Biology and Fisheries, 7(3): 331-363

O´LEARY, S.J.; HICE, L.A.; FELDHEIM, K.A.; FRISK,M.G.; MCELROY, A.E.; FAST, M.D.; CHAPMAN,
D.D. 2013 Severe Inbreeding and Small Effective Number of Breeders in a Formerly Abundant
Marine Fish. PLoS One, 8(6): 1-8.

OROZCO BERDUGO, G. and NARVÁEZ BARANDICA, J. C. 2014 Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia. Genetics and Molecular Biology, 37(1):37-45.

PESOA, N.A. and SCHULZ, U,H. 2010 Diel and seasonal movements of grumatã Prochilodus lineatus (Valenciennes 1836) (Characiformes: Prochilodontidae) in the Sinos River, Southern
Brazil. Brazilian Journal of Biology, 70(4): 1169-1177.

PETERSEN, R.S.; GARCIA, J.E.; MELLO, G.;LIEDKE, A.M.R.; SINCERO, T.C.M.; GRISARD, E.C. 2012 Análise da diversidade genética de tilápias cultivadas no estado de Santa Catarina (Brasil) utilizando marcadores microssatélites. Boletim do Instituto de Pesca, 38(4): 313-321.

POVH, J.A.; RIBEIRO, R.P.; SIROL, R.N.; STREIT JR,D.P.; MOREIRA, H.L.M.; SIEWERDT, F.;LOPERA BARRERO, N.M.; MANGOLIN, C.A.; VARGAS, L. 2010 Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the seminatural system of reproduction. Brazilian Archives of Biology and Technology, 53(2): 389-396.

POVH, J.A.; RIBEIRO, R.P.; LOPERA BARRERO, N.M.; JACOMETO, C.B.; VARGAS, L.; GOMES,P.C.; LOPES, T.S. 2011 Microsatellite analysis of pacu broodstocks used in the stocking program
of Paranapanema river, Brazil. Scientia Agricola,68(3): 308-313.

RAMELLA, M.S.; KROTH, M.A.; MEURER, S.;NUí­"˜ER, A.P.O.; ZANIBONI FILHO, E.; ARISI, A.C.M. 2006 Genetic variability in four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) from Uruguay River basin. Brazilian Archives of
Biology and Technology, 49(4): 589-598.

RICE, W.R. 1989 Analyzing table of statistical tests.Evolution, 43(1): 223-225

RIBOLLI, J.; MELO, C.M.R.; ZANIBONY-FILHO, E.2012 Genetic characterization of the Neotropical
catfish Pimelodus maculatus (Pimelodidae,Siluriformes) in the Upper Uruguay River.
Genetics and Molecular Biology, 35(4): 761-769.

RODRIGUEZ-RODRIGUEZ, M DEL P.; LOPERABARRERO, N.M.; RIBEIRO, R.P.; POVH, J.A.;VARGAS, L.; SIROL, R.N.; JACOMETO, C.B.2010 Genetic diversity of piracanjuba used in stock enhancement programs with microsatellite markers. Pesquisa Agropecuaria Brasiliera, 45(1):56-63.

ROUSSET, F. 2008 Genepop 2007: a complete reimplementation of the GENEPOP software for
Windows and Linux. Molecular Ecology Resources,8(1): 103-106.

RUEDA, E.; SOMMER, J.; SCARABOTTI, P.;MARKARIANI, R.; ORTÍ G. 2011 Isolation and characterization of polymorphic microsatellite loci in the migratory freshwater fish Prochilodus
lineatus (Characiformes, Prochilodontidae).Conservation Genetics Resources, 3(4): 681-684.

RUEDA, E.C.; CARRIQUIRIBORDE, P.; MONZÓN, A.M.; SOMOZA, G.M.; ORTÍ, G. 2013 Seasonal
variation in genetic population structure of sábalo (Prochilodus lineatus) in the Lower
Uruguay River. Genetica, 141(7-9): 401-407.

SANFORD, E. and KELLY, W. 2011 Local adaptation in marine invertebrates. Annual Review of Marine Science, 3: 509í 535.

SATAKE, A. and ARAKI, H. 2012 Stocking of captive-bred fish can cause long-term population decline and gene pool replacement:predictions from a population dynamics model
incorporating density-dependent mortality.Theoretical Ecology, 5(2): 283-296.

TAMURA, K.; PETERSON, D.; PETERSON, N.;STECHER, G.; NEI, M.; KUMAR, S. 2011

MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28(10): 2731-2739.

TOLEDO-FILHO, S.A. 1981 Biologia populacional do curimbatá (Prochilodus scrofa) Steindachner, 1881 (Pisces, Prochilodontidae) do rio Mogi-Guaçu:Aspectos quantitativos. São Paulo. 171p. (Tese deLivre-Docência, USP).

TOLEDO-FILHO, S.A.; GODOY, M.P.; SANTOS, E.P.1987 Delimitacão populacional do curimbatá,
Prochilodus scrofa (Pisces, Prochilodontidae) do Rio Mogi-Guaçu, Brasil. Revista Brasileira de
Biologia, 47(4): 501í 506

VAN OOSTERHOUT, C.; HUTCHINSON, W.F.D.; WILLS, P.M.; SHIPLEY, P. 2004 Micro-Checker:
software for identifying and correcting genotyping errors in microsatellite data.Molecular Ecology Notes, 4(3): 535-538.

WEIR, B.S. and COCKERHAM, C.C. 1984 Estimating F-statistics for the analysis of population
structure. Evolution, 38(6): 1358-1370.

WILLIAMS, S.E. and HOFFMAN, E.A. 2009 Minimizing genetic adaptation in captive breeding programs: A review. Biological Conservation, 142(11): 2388-2400.

WRIGHT S. 1978 Evolution and genetics of population.Chicago, IL: University of Chicago Press. 590p.

YAZBECK, G.M. and KALAPOTHAKIS, E. 2007 Isolation and characterization of microsatellite
DNA in the piracema fish Prochilodus lineatus (Characiformes). Genetics and Molecular Research,
6(4): 1026-1034.

YAMAMOTO, S.; MORITA, K.; KOIZUMI, I.;MAEKAWA, K. 2004 Genetic differentiation of
white-spotted charr (Salvelinus leucomaenis)populations after habitat fragmentation: Spatialí 
temporal changes in gene frequencies.Conservation Genetics, 5(4): 529-538.

YEH, F.C.; BOYLE, T.Y.Z.; XIYAN, J.M. 1999 PopGene Version 131: Microsoft Window-based freeware for population genetic analysis. Alberta, AB: University of Alberta and Center for International Forestry Research. Available at: <www.ualberta.ca/~fyeh/> Access on: 13 Mar.2014.

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2018-07-11

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