Diversidade genética de populações naturais e estoques de reprodutores de curimba para programas de repovoamento dos Rios Tietê, Grande, Pardo e Mogi-Guaçu (Brasil)
Palavras-chave:
Prochilodus lineatus, conservação genética, diferenciação genética, microssatélitesResumo
Prochilodus lineatus é um peixe tropical que nos últimos anos tem apresentado redução do número de populações selvagens. Programas de repovoamento foram desenvolvidos como método de conservação, e o monitoramento genético das populações e reprodutores é importante para assegurar a viabilidade de tais programas. O objetivo do estudo foi avaliar a diversidade genética de amostras selvagens (WSamp) e de reprodutores de P. lineatus do programa de repovoamento dos rios Tietê, Grande, Pardo e Mogi-Guaçu (São Paulo - Brasil), utilizando marcadores microssatélites. Alta variabilidade genética intrapopulacional foi observada. O número de alelos por loco variou de três a sete e foi detectada a diferenciação dos alelos entre WSamp e os reprodutores. Essa diferenciação foi confirmada por meio da análise do dendrograma. Os valores positivos da FIS indicaram a presença de endogamia em sete das 10 WSamp. A AMOVA e o FST indicaram pequena e moderada diferenciação genética entre as WSamp e alta diferenciação genética em comparação com os reprodutores. Esta diferenciação foi confirmada pelos valores de distí¢ncia e identidade genética e pelo número de indivíduos migrantes. Os resultados indicaram que houve alta variabilidade genética intrapopulacional, similaridade genética em WSamp e reprodutores e diferenciação entre WSamp e reprodutores. Pretende-se que este trabalho possa ser usado para auxiliar os programas de repovoamento de P. lineatus na região estudada e sirva como modelo de monitoramento para outros programas realizados no Brasil.
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population by a century-old dam. Hydrobiologia,638(1): 41-53.
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Bolívia, Argentina and Uruguay. FAO Fisheries Information, 490: 36-51.
EXCOFFIER, L.; LAVAL, G.; SCHNEIDER, S. 2005 Arlequin ver. 3.0: an integrated software
package for population genetics data analysis.Evolutionary Bioinformatics Online, 1: 47-50.
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FOPP-BAYAT, D. 2010 Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), cultured in a Polish fish farm.Acta Ichthyologica et Piscatoria, 40(1): 21í 25.
FOPP-BAYAT, D.; JANKUN, M.; KUZMINSKI, H.2010 Genetic characterization of Polish cultured
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