Control of Vibrio sp. using calcium hidroxide in pond of shrimp (Litopenaeus vannamei) culture in Santa Catarina
Keywords:
Vibrio, Litopenaeus vannamei, calcium hydroxideAbstract
Bacterial diseases, mainly those caused by bacteria of the Vibrio genus, are responsible for significant economic losses in the shrimp production. This study aimed to verify the concentration of calcium hydroxide [Ca(OH)2] able to inhibit growth of Vibrio harveyi in vitro, and also to test in vivo its use in culture ponds. For the in vitro experiment, bottles were used with BHI culture medium (Brain Heart Infusion) supplemented with Ca(OH)2 in four different concentrations (0; 0.018 g/L; 0.025 g/L and 0.036 g/L), inoculated with 1.2 x 1011 CFU/mL and incubated for 24 h under continuous agitation. The bacterial growth in the treatments was estimated by total counting of colonies in plates by successive dilutions (1/10). For the experiment in culture ponds, calcium hydroxide in the concentration of 0.018 g/L was applied for three consecutive days. Total counting of colonies in Marine Agar and TCBS (Thiosulphate-Citrate-Bile Salts-Sucrose) plates was done before and 48 h after calcium hydroxide application. The Vibrio concentration decreased significantly after the application, from 4.36 ± 0.1 (log UFC/mL ± DP) to 3.07 ± 0.36 (log UFC/mL ± DP). However, significant decrease in the concentration of total bacteria was not observed. In this way, it was evidenced that Ca(OH)2 can be used in the control of Vibrio spp. in rearing systems.
References
CHINABUT, S. 1994 EUS in Thailand. In: ROBERTS,R.J.; CAMPBELL, B.; MACREA, I.H. (Ed.).Proceedings of the ODA Regional Seminar on Epizootic Ulcerative Syndrome. Bangkok. p.58-60.
ESTRELA, C.; SYDNEY, G.B.; BAMMANN, L.; FELIPE,O. 1995 Mechanism of the action of calcium and hydroxyl ions of calcium hydroxide on tissue and bacteria. Brazilian Dental Journal, Ribeirão Preto,6(2): 85-90.
ESTRELA, C.; BAMMANN, L.L.; PIMENTA, F.C.;PÉCORA, J.D. 2001 Control of microorganisms in vitro by calcium hydroxide pastes. International Endodontic Journal, London, 34(5): 341-345.
FAIRWEATHER, D.J. 1999 Development of a bath challenge system to study component causes, and preventive treatments, of epizootic ulcerative syndrome (eus) in snakehead fish (Channa striata). Plymouth.
88p. (Dissertação de Mestrado. Universidade de Plymouth).
HOLMSTRí–M, K.; GRASLUND, S.; WAHLSTROM,A.; POUNGSHOMPOO, S.; BENGTSSON, B.E.; KAUTSKY, N. 2003 Antibiotic use in shrimp farming and implications for environmental impacts and human health. International Journal of Food Science and Technology, Oxford, 38(3):255-266.
LIGHTNER, D.V. e REDMAN, R.M. 1998 Shrimp diseases and current diagnostic methods. Aquaculture, Amsterdam, 164(4): 201-220.
PASHLEY, D., KALATHOOR, S., BURNHAM, D 1986 The effects of calcium hydroxide on dentin permeability. Journal of Dental Research, 65(3):417-420. RUANGPAN, L.; DANAYADOL, Y.; DIREKBUSARAKOM, S.; SIURAIRATANA, S.; FLEGEL, T. 1999 Lethal toxicity of Vibrio harveyi to cultivated Penaeus monodon induced by a bacteriophage. Diseases of Aquatic Organisms,Oldendorff, 35: 195-201.
SAFAVI, K. e NICHOLS, F.C. 1994 Alteration of biological properties of bacterial lipopolysaccharide by calcium hydroxide treatment. Journal of Endodontics, Baltimore, 20(3): 127-129.
SEUX, D.; COUBLE, M.L.; HARTMANN, D.J.;GAUTHIER, J.P.; MAGLOIRE, H. 1991 Odontoblast like cytodifferentiation of human pulp cells in vitro in the presence of a calcium hydroxide contamining cement. Archives of Oral Biology, 36(2): 117-128.
SKJERMO, J. e VADSTEIN, O. 1999 Techniques for microbial control in the intensive rearing of marine larvae. Aquaculture, Amsterdam, 177(1-4):333-343.
SONG, Y.L.; CHENG, W.; WANG, C.H. 1993 Isolation and characterization of Vibrio damsela infections for
cultured shrimp in Taiwan. Journal of Invertebrate Pathology, Nova York, 61(1): 24-31.
VINATEA, L. 2004 Princípios químicos de qualidade da água em aqüicultura: uma revisão para peixes e camarões.
2. ed. Florianópolis: Editora da UFSC. 231p.
WAKABAYASHI, H.; HORIKAWA, M.; FUNATO,A.; ONODERA, A.; MATSUMOTO, K. 1993 Bio-microscopical observation of dystrophic calcification induced by calcium hydroxide. Endodontics and Dental Traumatology, Copenhagen,9(4): 105-111.
