Post-mortem spermatophore and sperm cryopreservation of the white shrimp Litopenaeus schmitti
Keywords:
protocols, sperm mass, spermatophore, dimethylsulfoxide, glycerolAbstract
This study was carried out in two phases; the first, to evaluate the viability of two cooling protocols (A and B) using glycerol (10%) as a cryoprotectant, and the second, the efficiency of two cryoprotectants (10% glycerol and DMSO) and times (30, 60 and 90 days of storage in liquid nitrogen) for the cryopreservation of post-mortem sperm mass and spermatophores of the shrimp Litopenaeus schmitti. The protocol A presented a cooling rate of 0.5°C min-1 until reaching -32°C and protocol B, 20°C min-1 until reaching -120°C, after which the samples were transferred to liquid nitrogen (N2L). The sperm viability was assessed by smearing the semen stained with eosine-nigrosine. The curve that resulted in higher mean sperm survival was of Protocol A (50.9%). Therefore, for the second experiment, the protocol A was used, however there was no difference between the cryoprotectants for sperm mass, although the influence on survival time. Difference was observed between the cryoprotectants and the influence of time on sperm survival of cryopreserved spermatophores. Glycerol 10% was more efficient for cryopreservation of spermatophore, but for the masses sperm both can be used.
References
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ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J.; CLARK, W.H. 1988 Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, 25: 238í 43.
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BEHLMER, S.D.; BROWN, G. 1984 Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, 7: 193-199.
BRAVANISHANKAR, S. e SUBRAMONIAM, T. 1997 Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, 277: 326-336.
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Aquaculture, 160: 63í 67.
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Cryopreservation and sperm storage of the white shrimp. Boletim do Instituto de Pesca, 40(1):17-22.
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JEYALECTUMIE, C. e SUBRAMONIAM, T. 1989 Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, 177: 247-253.
LEENA GRACE, B. e NATARAJAN, P. 2003 Cryopreservation of Penaeus monodon (Fabricius,1798) spermatozoa. Indian Journal of Fisheries,50(3): 415-419.
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PARKS, J.E. e GRAHAM, J.K. 1992 Effects of cryopreservation procedures on sperm
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SANTOS, R.R.; CELESTINO, J.J.H.; LOPES, C.A.P.; MELO, M.A.P.; RODRIGUES, A.P.R.;
FIGUEIREDO, JR. 2008 Criopreservação de folículos ovarianos pré-antrais de animais
domésticos. Revista Brasileira de Reprodução Animal, 32: 9-15.
UFV - UNIVERSIDADE FEDERAL DE VIí"¡OSA.2007 SAEG í Sistema de análises estatísticas e genéticas. Viçosa: Universidade Federal de Viçosa, 2007. Manual do usuário, 150p. (versão 9.1).
VERSTEGEN, J.; IGUER-OUADA, M.; ONCLIN, K.2002 Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57: 149-179.
VUTHIPHANDCHAI, V.; NIMRAT, S.;KOTCHARAT S.; BART, A.N. 2007 Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores.Theriogenology, 68: 1192í 1199.
WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. 1995 Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society,
26: 261-271.
YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. 1987 Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation.Canadian Journal of Zoology, 65: 9-13
cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research, 35: 1415í 1420.
ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J.; CLARK, W.H. 1988 Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, 25: 238í 43.
ARCE, S.M.; MOSS, S.M.; ARGUE, B.J. 1999 Artificial insemination and spawning of pacific white shrimp Litopenaeus vannamei: implications for a selective breeding program. UJNR Technical Report, 28: 5-8.
BART, A.N.; CHOOSUK, S.; THAKUR, D.P. 2006 Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research, 37:523-528.
BEHLMER, S.D.; BROWN, G. 1984 Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, 7: 193-199.
BRAVANISHANKAR, S. e SUBRAMONIAM, T. 1997 Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, 277: 326-336.
BRAY, W.A. e LAWRENCE, A.L. 1998 Male viability determinations in Penaeus vannamei: evaluation of short-term storage of spermatophores up to 36 h and comparison of Ca-free saline and seawater as sperm homogenate media.
Aquaculture, 160: 63í 67.
CASTELO-BRANCO, T.C.; BAMBOZZI, A.F.; OSHIRO, L.M.Y.; MELLO, M.R.B. 2014
Cryopreservation and sperm storage of the white shrimp. Boletim do Instituto de Pesca, 40(1):17-22.
CHOW, S.; TAKI, Y.; OGASAWARA, Y. 1985 Cryopreservation of spermatophore of the fresh water shrimp, Macrobrachium rosenbergii. The Biological Bulletin, 168: 471í 5.
ELMOAZZEN, H.Y. 2000 Parameters affecting water permeability across biological cell membranes. 141p.- Dissertação de mestrado. University of Alberta Edmonton). Disponível em:http://www.collectionscanada.gc.ca/obj/s4/f2/dsk1/tape3/PQDD_0009/MQ60118.pdf
FRIEDLER, S.; GIUDICE, S.C.; LAMB, E.J. 1988 Cryopreservation of embryos and ova. Fertility and Sterility, 49: 743-764.
FULLER, B.J.; LANE, N.; BENSON, E.E. 2004 Life in the frozen state. In: MAZUR, P. Principles of Cryobiology. CRC Press. p.3-65.
GONí"¡ALVES, P.B.D.; FIGUEIREDO, J.R.; FREITAS,V.J.F. 2008 Biotécnicas aplicadas í reprodução animal. 2a ed. São Paulo: Roca. 408p
GWO, J.C. 2000 Cryopresevation of aquatic invertebrate semen: a review. Aquaculture Research, 31: 259-271.
HOVATTA, O. 2005 Methods for cryopreservation of human ovarian tissue. Reproductive Bio Medicine Online, 10: 729-734.
JEYALECTUMIE, C. e SUBRAMONIAM, T. 1989 Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, 177: 247-253.
LEENA GRACE, B. e NATARAJAN, P. 2003 Cryopreservation of Penaeus monodon (Fabricius,1798) spermatozoa. Indian Journal of Fisheries,50(3): 415-419.
LEZCANO, M.; GRANJA, C.; SALAZAR, M. 2004 The use of flow cytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp (Litopenaeus vannamei). Cryobiology, 48: 349í 56.
MATOS, D.L.; ARAíÅ¡JO, A.A.; ROBERTO, I.G.;TONIOLLI, R. 2008 Análise computarizada de espermatozóides: revisão de literatura. Revista Brasileira de Reprodução Animal, 32(4): 225-232.
MAZUR, P. 1985 Basic concepts in freezing cells. In: JOHNSON L.A. e LARSSON K. (eds.)Proceedings of the First International Conference on Deep Freezing of Boar Semen. Uppsala: Swedish University of Agricultural Sciences. p.91í 111.
MAZUR, P.; RALL, W.F.; LEIBO, S.P. 1984 Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophysics, 6: 197-213.
MEMON, A.J.; TALPUR, A.D.; KHAN, M.I.; FARIDDUDIN, M.O.; SAFIAH, J. 2012
Optimization of spermatophores cryopreservation protocolo of banana shrimp
(Penaeus merguiensis) (De Man, 1888). Journal of Animal and Veterinary Advances, 11(10): 1688-1704.
MONTOYA, J.M. 2001 Controlled reproduction of penaeid shrimp: a contribution to its improvment.149p.
NIMRAT, S.; SIRIBOONLAMOM, S.; ZHANG, S.; XU, Y.; VUTHIPHANDCHAI, V. 2006 Chilled storage of white shrimp (L. vannamei) spermatophores. Aquaculture, 261: 944í 951.
PARKS, J.E. e GRAHAM, J.K. 1992 Effects of cryopreservation procedures on sperm
membranes. Theriogenology, 38: 209í 222.
PÉREZ-JAR, L. 2005 Fisiología y calidad reproductiva de machos de camarón blanco Litopenaeus schmitti en condiciones de cautiverio. La Paz.150p. (Tese de Doutorado. Centro de Investigaciones Biológicas del Noroeste).
Disponível em: http://www.oceandocs.org/bitstream/1834/1544/1/Tesis_Doctorado_Lourdes_Perez _Jar.pdf
PONGTIPPATEE, P.; VANICHVIRIYAKIT, R.; CHAVADEJ, J.; PLODPAI, P.; PRATOOMCHART, B.; SOBHON, P.;WITHYACHUMNARNKUL, B. 2007 Acrosome
reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae).Aquaculture Research, 38: 1635-1644.
SALAZAR, M.; LEZCANO, M.; GRANJA, C. 2008 Protocol for cryopreservation of Penaeus vannamei sperm cells. In: CABRITA, E.; ROBLES, V.; HERRÁEZ, P. Methods in Reproductive Aquaculture. Marine and Freshwater Species. Boca raton, FL, CRC Press, p.505-508.
SANTOS, R.R.; CELESTINO, J.J.H.; LOPES, C.A.P.; MELO, M.A.P.; RODRIGUES, A.P.R.;
FIGUEIREDO, JR. 2008 Criopreservação de folículos ovarianos pré-antrais de animais
domésticos. Revista Brasileira de Reprodução Animal, 32: 9-15.
UFV - UNIVERSIDADE FEDERAL DE VIí"¡OSA.2007 SAEG í Sistema de análises estatísticas e genéticas. Viçosa: Universidade Federal de Viçosa, 2007. Manual do usuário, 150p. (versão 9.1).
VERSTEGEN, J.; IGUER-OUADA, M.; ONCLIN, K.2002 Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57: 149-179.
VUTHIPHANDCHAI, V.; NIMRAT, S.;KOTCHARAT S.; BART, A.N. 2007 Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores.Theriogenology, 68: 1192í 1199.
WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. 1995 Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society,
26: 261-271.
YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. 1987 Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation.Canadian Journal of Zoology, 65: 9-13
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2018-11-15
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