Criopreservação do espermatóforo e da massa espermática do camarão branco Litopenaeus schmitti post-mortem
Palavras-chave:
protocolos, massa espermática, espermatóforo, dimetilsulfóxido, glicerolResumo
O presente trabalho foi realizado em duas etapas; a primeira, para avaliar a eficiência de dois protocolos de resfriamento (A e B) utilizando glicerol (10%) como crioprotetor e a segunda, a eficiência de dois crioprotetores (glicerol e dimetilsulfóxido - DMSO a 10%) e o tempo (30, 60 e 90 dias de estocagem em nitrogênio líquido) para a criopreservação da massa espermática e espermatóforo de camarões post mortem da espécie Litopenaeus schmitti. O protocolo A apresentou velocidade de resfriamento de 0,5°C min-1 até alcançar -32°C e o protocolo B, 20°C min-1 até alcançar -120°C, sendo os materiais posteriormente transferidos para nitrogênio líquido (N2L). A viabilidade espermática foi avaliada por meio do esfregaço de sêmen corado com eosina-nigrosina. A curva que resultou na maior média de sobrevivência espermática foi a do protocolo A (50,9%). Portanto, para o segundo experimento foi utilizado o protocolo A; entretanto, neste não houve diferença entre os crioprotetores para a massa espermática, contudo, o tempo influenciou na sobrevivência. Foi observada diferença entre os crioprotetores e a influência do tempo na sobrevivência espermática dos espermatóforos criopreservados. O glicerol a 10% foi mais eficiente para a criopreservação dos espermatóforos, porém, para as massas espermáticas, ambos podem ser utilizados.
Referências
AKARASANON, K.; DAMRONGPHOL, P.;POOLSANGUAN, W. 2004 Long-term
cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research, 35: 1415í 1420.
ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J.; CLARK, W.H. 1988 Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, 25: 238í 43.
ARCE, S.M.; MOSS, S.M.; ARGUE, B.J. 1999 Artificial insemination and spawning of pacific white shrimp Litopenaeus vannamei: implications for a selective breeding program. UJNR Technical Report, 28: 5-8.
BART, A.N.; CHOOSUK, S.; THAKUR, D.P. 2006 Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research, 37:523-528.
BEHLMER, S.D.; BROWN, G. 1984 Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, 7: 193-199.
BRAVANISHANKAR, S. e SUBRAMONIAM, T. 1997 Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, 277: 326-336.
BRAY, W.A. e LAWRENCE, A.L. 1998 Male viability determinations in Penaeus vannamei: evaluation of short-term storage of spermatophores up to 36 h and comparison of Ca-free saline and seawater as sperm homogenate media.
Aquaculture, 160: 63í 67.
CASTELO-BRANCO, T.C.; BAMBOZZI, A.F.; OSHIRO, L.M.Y.; MELLO, M.R.B. 2014
Cryopreservation and sperm storage of the white shrimp. Boletim do Instituto de Pesca, 40(1):17-22.
CHOW, S.; TAKI, Y.; OGASAWARA, Y. 1985 Cryopreservation of spermatophore of the fresh water shrimp, Macrobrachium rosenbergii. The Biological Bulletin, 168: 471í 5.
ELMOAZZEN, H.Y. 2000 Parameters affecting water permeability across biological cell membranes. 141p.- Dissertação de mestrado. University of Alberta Edmonton). Disponível em:http://www.collectionscanada.gc.ca/obj/s4/f2/dsk1/tape3/PQDD_0009/MQ60118.pdf
FRIEDLER, S.; GIUDICE, S.C.; LAMB, E.J. 1988 Cryopreservation of embryos and ova. Fertility and Sterility, 49: 743-764.
FULLER, B.J.; LANE, N.; BENSON, E.E. 2004 Life in the frozen state. In: MAZUR, P. Principles of Cryobiology. CRC Press. p.3-65.
GONí"¡ALVES, P.B.D.; FIGUEIREDO, J.R.; FREITAS,V.J.F. 2008 Biotécnicas aplicadas í reprodução animal. 2a ed. São Paulo: Roca. 408p
GWO, J.C. 2000 Cryopresevation of aquatic invertebrate semen: a review. Aquaculture Research, 31: 259-271.
HOVATTA, O. 2005 Methods for cryopreservation of human ovarian tissue. Reproductive Bio Medicine Online, 10: 729-734.
JEYALECTUMIE, C. e SUBRAMONIAM, T. 1989 Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, 177: 247-253.
LEENA GRACE, B. e NATARAJAN, P. 2003 Cryopreservation of Penaeus monodon (Fabricius,1798) spermatozoa. Indian Journal of Fisheries,50(3): 415-419.
LEZCANO, M.; GRANJA, C.; SALAZAR, M. 2004 The use of flow cytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp (Litopenaeus vannamei). Cryobiology, 48: 349í 56.
MATOS, D.L.; ARAíÅ¡JO, A.A.; ROBERTO, I.G.;TONIOLLI, R. 2008 Análise computarizada de espermatozóides: revisão de literatura. Revista Brasileira de Reprodução Animal, 32(4): 225-232.
MAZUR, P. 1985 Basic concepts in freezing cells. In: JOHNSON L.A. e LARSSON K. (eds.)Proceedings of the First International Conference on Deep Freezing of Boar Semen. Uppsala: Swedish University of Agricultural Sciences. p.91í 111.
MAZUR, P.; RALL, W.F.; LEIBO, S.P. 1984 Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophysics, 6: 197-213.
MEMON, A.J.; TALPUR, A.D.; KHAN, M.I.; FARIDDUDIN, M.O.; SAFIAH, J. 2012
Optimization of spermatophores cryopreservation protocolo of banana shrimp
(Penaeus merguiensis) (De Man, 1888). Journal of Animal and Veterinary Advances, 11(10): 1688-1704.
MONTOYA, J.M. 2001 Controlled reproduction of penaeid shrimp: a contribution to its improvment.149p.
NIMRAT, S.; SIRIBOONLAMOM, S.; ZHANG, S.; XU, Y.; VUTHIPHANDCHAI, V. 2006 Chilled storage of white shrimp (L. vannamei) spermatophores. Aquaculture, 261: 944í 951.
PARKS, J.E. e GRAHAM, J.K. 1992 Effects of cryopreservation procedures on sperm
membranes. Theriogenology, 38: 209í 222.
PÉREZ-JAR, L. 2005 Fisiología y calidad reproductiva de machos de camarón blanco Litopenaeus schmitti en condiciones de cautiverio. La Paz.150p. (Tese de Doutorado. Centro de Investigaciones Biológicas del Noroeste).
Disponível em: http://www.oceandocs.org/bitstream/1834/1544/1/Tesis_Doctorado_Lourdes_Perez _Jar.pdf
PONGTIPPATEE, P.; VANICHVIRIYAKIT, R.; CHAVADEJ, J.; PLODPAI, P.; PRATOOMCHART, B.; SOBHON, P.;WITHYACHUMNARNKUL, B. 2007 Acrosome
reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae).Aquaculture Research, 38: 1635-1644.
SALAZAR, M.; LEZCANO, M.; GRANJA, C. 2008 Protocol for cryopreservation of Penaeus vannamei sperm cells. In: CABRITA, E.; ROBLES, V.; HERRÁEZ, P. Methods in Reproductive Aquaculture. Marine and Freshwater Species. Boca raton, FL, CRC Press, p.505-508.
SANTOS, R.R.; CELESTINO, J.J.H.; LOPES, C.A.P.; MELO, M.A.P.; RODRIGUES, A.P.R.;
FIGUEIREDO, JR. 2008 Criopreservação de folículos ovarianos pré-antrais de animais
domésticos. Revista Brasileira de Reprodução Animal, 32: 9-15.
UFV - UNIVERSIDADE FEDERAL DE VIí"¡OSA.2007 SAEG í Sistema de análises estatísticas e genéticas. Viçosa: Universidade Federal de Viçosa, 2007. Manual do usuário, 150p. (versão 9.1).
VERSTEGEN, J.; IGUER-OUADA, M.; ONCLIN, K.2002 Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57: 149-179.
VUTHIPHANDCHAI, V.; NIMRAT, S.;KOTCHARAT S.; BART, A.N. 2007 Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores.Theriogenology, 68: 1192í 1199.
WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. 1995 Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society,
26: 261-271.
YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. 1987 Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation.Canadian Journal of Zoology, 65: 9-13
cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Aquaculture Research, 35: 1415í 1420.
ANCHORDOGUY T.; CROWE J.H.; GRIFFIN F.J.; CLARK, W.H. 1988 Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, 25: 238í 43.
ARCE, S.M.; MOSS, S.M.; ARGUE, B.J. 1999 Artificial insemination and spawning of pacific white shrimp Litopenaeus vannamei: implications for a selective breeding program. UJNR Technical Report, 28: 5-8.
BART, A.N.; CHOOSUK, S.; THAKUR, D.P. 2006 Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius). Aquaculture Research, 37:523-528.
BEHLMER, S.D.; BROWN, G. 1984 Viability of cryopreserved spermatozoa of the horseshoe crab, Limulus polyphemus L. International Journal Invertebrate Reproduction Development, 7: 193-199.
BRAVANISHANKAR, S. e SUBRAMONIAM, T. 1997 Cryopreservation of spermatozoa of the edible mud crab Scylla serrata (Forskal). Journal of Experimental Zoology, 277: 326-336.
BRAY, W.A. e LAWRENCE, A.L. 1998 Male viability determinations in Penaeus vannamei: evaluation of short-term storage of spermatophores up to 36 h and comparison of Ca-free saline and seawater as sperm homogenate media.
Aquaculture, 160: 63í 67.
CASTELO-BRANCO, T.C.; BAMBOZZI, A.F.; OSHIRO, L.M.Y.; MELLO, M.R.B. 2014
Cryopreservation and sperm storage of the white shrimp. Boletim do Instituto de Pesca, 40(1):17-22.
CHOW, S.; TAKI, Y.; OGASAWARA, Y. 1985 Cryopreservation of spermatophore of the fresh water shrimp, Macrobrachium rosenbergii. The Biological Bulletin, 168: 471í 5.
ELMOAZZEN, H.Y. 2000 Parameters affecting water permeability across biological cell membranes. 141p.- Dissertação de mestrado. University of Alberta Edmonton). Disponível em:http://www.collectionscanada.gc.ca/obj/s4/f2/dsk1/tape3/PQDD_0009/MQ60118.pdf
FRIEDLER, S.; GIUDICE, S.C.; LAMB, E.J. 1988 Cryopreservation of embryos and ova. Fertility and Sterility, 49: 743-764.
FULLER, B.J.; LANE, N.; BENSON, E.E. 2004 Life in the frozen state. In: MAZUR, P. Principles of Cryobiology. CRC Press. p.3-65.
GONí"¡ALVES, P.B.D.; FIGUEIREDO, J.R.; FREITAS,V.J.F. 2008 Biotécnicas aplicadas í reprodução animal. 2a ed. São Paulo: Roca. 408p
GWO, J.C. 2000 Cryopresevation of aquatic invertebrate semen: a review. Aquaculture Research, 31: 259-271.
HOVATTA, O. 2005 Methods for cryopreservation of human ovarian tissue. Reproductive Bio Medicine Online, 10: 729-734.
JEYALECTUMIE, C. e SUBRAMONIAM, T. 1989 Cryopreservation of spermatophores and seminal plasma of the edible crab Scylla serrata. Biological Bulletin, 177: 247-253.
LEENA GRACE, B. e NATARAJAN, P. 2003 Cryopreservation of Penaeus monodon (Fabricius,1798) spermatozoa. Indian Journal of Fisheries,50(3): 415-419.
LEZCANO, M.; GRANJA, C.; SALAZAR, M. 2004 The use of flow cytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp (Litopenaeus vannamei). Cryobiology, 48: 349í 56.
MATOS, D.L.; ARAíÅ¡JO, A.A.; ROBERTO, I.G.;TONIOLLI, R. 2008 Análise computarizada de espermatozóides: revisão de literatura. Revista Brasileira de Reprodução Animal, 32(4): 225-232.
MAZUR, P. 1985 Basic concepts in freezing cells. In: JOHNSON L.A. e LARSSON K. (eds.)Proceedings of the First International Conference on Deep Freezing of Boar Semen. Uppsala: Swedish University of Agricultural Sciences. p.91í 111.
MAZUR, P.; RALL, W.F.; LEIBO, S.P. 1984 Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophysics, 6: 197-213.
MEMON, A.J.; TALPUR, A.D.; KHAN, M.I.; FARIDDUDIN, M.O.; SAFIAH, J. 2012
Optimization of spermatophores cryopreservation protocolo of banana shrimp
(Penaeus merguiensis) (De Man, 1888). Journal of Animal and Veterinary Advances, 11(10): 1688-1704.
MONTOYA, J.M. 2001 Controlled reproduction of penaeid shrimp: a contribution to its improvment.149p.
NIMRAT, S.; SIRIBOONLAMOM, S.; ZHANG, S.; XU, Y.; VUTHIPHANDCHAI, V. 2006 Chilled storage of white shrimp (L. vannamei) spermatophores. Aquaculture, 261: 944í 951.
PARKS, J.E. e GRAHAM, J.K. 1992 Effects of cryopreservation procedures on sperm
membranes. Theriogenology, 38: 209í 222.
PÉREZ-JAR, L. 2005 Fisiología y calidad reproductiva de machos de camarón blanco Litopenaeus schmitti en condiciones de cautiverio. La Paz.150p. (Tese de Doutorado. Centro de Investigaciones Biológicas del Noroeste).
Disponível em: http://www.oceandocs.org/bitstream/1834/1544/1/Tesis_Doctorado_Lourdes_Perez _Jar.pdf
PONGTIPPATEE, P.; VANICHVIRIYAKIT, R.; CHAVADEJ, J.; PLODPAI, P.; PRATOOMCHART, B.; SOBHON, P.;WITHYACHUMNARNKUL, B. 2007 Acrosome
reaction in the sperm of the black tiger shrimp Penaeus monodon (Decapoda, Penaeidae).Aquaculture Research, 38: 1635-1644.
SALAZAR, M.; LEZCANO, M.; GRANJA, C. 2008 Protocol for cryopreservation of Penaeus vannamei sperm cells. In: CABRITA, E.; ROBLES, V.; HERRÁEZ, P. Methods in Reproductive Aquaculture. Marine and Freshwater Species. Boca raton, FL, CRC Press, p.505-508.
SANTOS, R.R.; CELESTINO, J.J.H.; LOPES, C.A.P.; MELO, M.A.P.; RODRIGUES, A.P.R.;
FIGUEIREDO, JR. 2008 Criopreservação de folículos ovarianos pré-antrais de animais
domésticos. Revista Brasileira de Reprodução Animal, 32: 9-15.
UFV - UNIVERSIDADE FEDERAL DE VIí"¡OSA.2007 SAEG í Sistema de análises estatísticas e genéticas. Viçosa: Universidade Federal de Viçosa, 2007. Manual do usuário, 150p. (versão 9.1).
VERSTEGEN, J.; IGUER-OUADA, M.; ONCLIN, K.2002 Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57: 149-179.
VUTHIPHANDCHAI, V.; NIMRAT, S.;KOTCHARAT S.; BART, A.N. 2007 Development of a cryopreservation protocol for long-term storage of black tiger shrimp (Penaeus monodon) spermatophores.Theriogenology, 68: 1192í 1199.
WANG, Q.Y.; MISAMORE, M.; JIANG, C.Q.; BROWDY, C.L. 1995 Egg water induced reaction and biostain assay of sperm from marine shrimp Penaeus vannamei: dietary effects on sperm quality. Journal of the World Aquaculture Society,
26: 261-271.
YOO, B.Y.; RYAN, M.A.; WIGGS, A.J. 1987 Loss of protein from spermatozoa of Atlantic salmon (Salmo salar L.) because of cryopreservation.Canadian Journal of Zoology, 65: 9-13
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2018-11-15
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