A suplementação alimentar com vitamina C acelera a evolução do processo cicatricial em Piaractus mesopotamicus (Holmberg, 1887)
Palavras-chave:
cicatrização, vitamina C (ácido ascórbico), Piaractus mesopotamicusResumo
Neste ensaio estudou-se, comparativamente, a cinética da evolução do processo cicatricial em pacu Piaractus mesopotamicus Holmberg, 1887 (Osteichthyes: Characidae) alimentado com ração não suplementada (controle) e suplementada com 100, 200 e 500 mg de vitamina C/kg de ração. Feridas cutí¢neas (15 mm x 10 mm x 0,25 mm) foram realizadas com a remoção da epiderme e derme, e a evolução do processo cicatricial foi avaliada macro e microscopicamente 1, 3, 7, 14, 21, 28 e 35 dias depois. Os resultados demonstram que as características morfológicas diferiram entre os tratamentos, porque a vitamina C promoveu a aceleração do fenômeno, através do aumento da proliferação de células mucosas, do acúmulo de colágeno, da remodelação tecidual e da formação de escamas, consequentemente, levando í redução proporcional da área lesada.
Referências
AGRAWAL, N.K. e MAHAJAN, C.L. 1980 Nutritional deficiency disease in an Indian major carp Cirrhina mrigala Hamilton, due to avitaminosis C during early growth. J. Fish Dis., 3: 231-248.
ANDERSON, C.D. e ROBERTS, R.J. 1975 A comparision of the effects of temperature on wound healing in a tropical and a temperate teleost. J. Fish Biol., 7: 173-182.
AOAC - Association Official Agriculture Chemists 1975 Official Methods of Analysis. 3. ed.Washington. 975p.
BANZATTO, D. A. e KRONKA, S.N. 1989 Experimentação Agrícola. Jaboticabal: FUNEP.247p.
BEREITER-HAHN, J. 1986 Epidermal cell migration and wound repair. In: Biology of the Integument, V.2 -Vertebrates. Heidelberg: Springer.p.443-471.
BULLOCK, A M.; MARKS, R.; ROBERTS, R.J. 1978a The cell kinetics of teleost fish epidermis: mitotic activity of the normal epidermis at varying temperatures in plaice (Pleuronectes platessa).J. Zool. Lond., 184: 423-428.
BULLOCK, A M.; MARKS, R; ROBERTS, R.J. 1978b The cell kinetics of teleost fish epidermis: epidermal mitotic activity in relation to wound healing at varying temperatures in plaice (Pleuronectes platessa). J. Zool. Lond., 185: 197-204.
CHAVEZ DE MARTINEZ, M.C. e RICHARDS, R.H. 1991 Histopathology of vitamin C deficiency in a cichlid Cichlasoma urophthalmus (Günther). J. Fish Dis.,.14: 507-519.
DURVE, V.S. e LOVELL, R.T. 1982 Vitamin C and disease resistance in channel catfish Ictalurus punctatus. J. Fish. Aquat. Sci., 39: 948-951.
DUTTA, M. e RAI, A.K. 1994 Pattern of cutaneous wound healig in a live fish Clarias batrachus (L) (Clariidae, Pisces). J. Ind. Fish. Assoc., 24: 107-113.
HALVER, J.E. 1953 Fish diseases and nutrition.Trans. Am. Fish. Soc., 83: 254-261.
HALVER, J.E.; SMITH, R.R.; TOLBERT, B.M.; BAKER, L.M. 1975 Utilization of ascorbic acid in fish.Ann. N. Y. Acad. Sci., 258: 81-102.
IGER, Y. e ABRAHAM, M. 1990 The process of skin healing in experimentally wounded carp. J. Fish Biol., 36: 421-437.
IGER, Y.; ABRAHAM, M.; DOTAM, A.; FATTAL, B.; RAHAMIN, E. 1988 Cellular responses in the skin of carp maintained in organically fertilyzed water. J. Fish Biol., 33: 711-720.
INSTITUTO ADOLFO LUTZ 1985 Normas Analíticas: método químico e físico para análise de alimentos. 4 ed. São Paulo. p.345-420.
JAUNCEY, K.; SOLIMAN, A; ROBERTS, J.R. 1985 Ascorbic acid requirements in relation to wound healing in the cultured tilapia Oreochromis niloticus (TREWAVAS). Aquacult. Fish Manage.,16: 139-149.
LI, Y.P. e LOVELL, R.T. 1985 Elevated levels of dietary ascorbic acid increase immune responses in channel catfish. J. Nutr., 115: 123-131.
LOVEL, R.T. 1982 Elevated levels of vitamin C increase disease resistance in channel catfish.Highlights Agric. Res., 29: 124-131.
MARTINS, M.L. 1995 Effect of ascorbic acid deficiency on the growth, gill filament lesions and behavior of pacu fry (Piaractus mesopotamicus, Holmberg, 1887). Braz. J. Med. Biol. Res.,28: 563-568.
MARTINS, M.L. 1998 Evaluation of the addition of ascorbic acid to the ration of cultivated Piaractus mesopotamicus (Characidae) on the infrapopulation of Anacanthorus penilabiatus (Monogenea). Braz. J. Med. Biol. Res.: 31: 655-658.
MARTINS, M.L. 2000 Efeito da suplementação com vitamina C sobre a reação inflamatória em Piaractus mesopotamicus Holmberg, 1887 estressados. Unesp - Jaboticabal 125p. (Tese de Doutoramento.Centro de Aqüicultura, Unesp, Jaboticabal, SP).
MITOMA, C. e SMITH, T.E. 1960 Studies on the role of ascorbic acid in collagen synthesis.J. Biol. Chem., 235: 426-428.
MITTAL, A.K. e MUNSHI, J.S.D. 1974 On the regeneration and repair of superficial wound in the skin of Rita rita (Ham.) (Bagridae, Pisces).Acta Anat., 88: 424-442.
NOGA, E.J. 1996 Fish Disease. Diagnosis and Treatment. 1a ed. Raleigh: Mosby. 367p.
PETRIC, M.C. 2000 Efeito da suplementação alimentar com vitamina C sobre a formação de gigantócitos em
lamínulas de vidro implantadas no tecido subcutí¢neo de pacus (Piaractus mesopotamicus Holmberg,1887). Jaboticabal-Unesp. 86p. (Dissertação de Mestrado. Faculdade de Ciências Agrárias e Veterinárias, Unesp).
PHROMSUTHIRAK, P. 1977 Electron microscopy of wound healing in the skin of Gasterosteus aculeatus. J. Fish Biol., 11: 193-206.
QUILHAC, A. e SIRE, S.Y. 1999 Spreading,proliferation, and differentiation of the epidermis after wounding a Cichlid fish, Hemichromis bimaculatus. Anat. Rec., 254: 435-451.
RANGANNA, S. 1977 Manual of analysis of fruit and vegetable products. New Delhi: McGraw-Hill.634p.
RYAN, G.B. e SPECTOR, W.G. 1970 Macrophage turnover in inflammed connective tissues. Proc.R. Soc. Lon., ser. B:175: 269-292.
WAHLI, T.; MEIER, W.; PFISTER, K. 1986 Ascorbic acid induced immune mediated decrease in mortality in Ichthyophthirius multifiliis infected rainbow trout (Salmo gairdneri). Acta Trop.,43: 287-289.
ANDERSON, C.D. e ROBERTS, R.J. 1975 A comparision of the effects of temperature on wound healing in a tropical and a temperate teleost. J. Fish Biol., 7: 173-182.
AOAC - Association Official Agriculture Chemists 1975 Official Methods of Analysis. 3. ed.Washington. 975p.
BANZATTO, D. A. e KRONKA, S.N. 1989 Experimentação Agrícola. Jaboticabal: FUNEP.247p.
BEREITER-HAHN, J. 1986 Epidermal cell migration and wound repair. In: Biology of the Integument, V.2 -Vertebrates. Heidelberg: Springer.p.443-471.
BULLOCK, A M.; MARKS, R.; ROBERTS, R.J. 1978a The cell kinetics of teleost fish epidermis: mitotic activity of the normal epidermis at varying temperatures in plaice (Pleuronectes platessa).J. Zool. Lond., 184: 423-428.
BULLOCK, A M.; MARKS, R; ROBERTS, R.J. 1978b The cell kinetics of teleost fish epidermis: epidermal mitotic activity in relation to wound healing at varying temperatures in plaice (Pleuronectes platessa). J. Zool. Lond., 185: 197-204.
CHAVEZ DE MARTINEZ, M.C. e RICHARDS, R.H. 1991 Histopathology of vitamin C deficiency in a cichlid Cichlasoma urophthalmus (Günther). J. Fish Dis.,.14: 507-519.
DURVE, V.S. e LOVELL, R.T. 1982 Vitamin C and disease resistance in channel catfish Ictalurus punctatus. J. Fish. Aquat. Sci., 39: 948-951.
DUTTA, M. e RAI, A.K. 1994 Pattern of cutaneous wound healig in a live fish Clarias batrachus (L) (Clariidae, Pisces). J. Ind. Fish. Assoc., 24: 107-113.
HALVER, J.E. 1953 Fish diseases and nutrition.Trans. Am. Fish. Soc., 83: 254-261.
HALVER, J.E.; SMITH, R.R.; TOLBERT, B.M.; BAKER, L.M. 1975 Utilization of ascorbic acid in fish.Ann. N. Y. Acad. Sci., 258: 81-102.
IGER, Y. e ABRAHAM, M. 1990 The process of skin healing in experimentally wounded carp. J. Fish Biol., 36: 421-437.
IGER, Y.; ABRAHAM, M.; DOTAM, A.; FATTAL, B.; RAHAMIN, E. 1988 Cellular responses in the skin of carp maintained in organically fertilyzed water. J. Fish Biol., 33: 711-720.
INSTITUTO ADOLFO LUTZ 1985 Normas Analíticas: método químico e físico para análise de alimentos. 4 ed. São Paulo. p.345-420.
JAUNCEY, K.; SOLIMAN, A; ROBERTS, J.R. 1985 Ascorbic acid requirements in relation to wound healing in the cultured tilapia Oreochromis niloticus (TREWAVAS). Aquacult. Fish Manage.,16: 139-149.
LI, Y.P. e LOVELL, R.T. 1985 Elevated levels of dietary ascorbic acid increase immune responses in channel catfish. J. Nutr., 115: 123-131.
LOVEL, R.T. 1982 Elevated levels of vitamin C increase disease resistance in channel catfish.Highlights Agric. Res., 29: 124-131.
MARTINS, M.L. 1995 Effect of ascorbic acid deficiency on the growth, gill filament lesions and behavior of pacu fry (Piaractus mesopotamicus, Holmberg, 1887). Braz. J. Med. Biol. Res.,28: 563-568.
MARTINS, M.L. 1998 Evaluation of the addition of ascorbic acid to the ration of cultivated Piaractus mesopotamicus (Characidae) on the infrapopulation of Anacanthorus penilabiatus (Monogenea). Braz. J. Med. Biol. Res.: 31: 655-658.
MARTINS, M.L. 2000 Efeito da suplementação com vitamina C sobre a reação inflamatória em Piaractus mesopotamicus Holmberg, 1887 estressados. Unesp - Jaboticabal 125p. (Tese de Doutoramento.Centro de Aqüicultura, Unesp, Jaboticabal, SP).
MITOMA, C. e SMITH, T.E. 1960 Studies on the role of ascorbic acid in collagen synthesis.J. Biol. Chem., 235: 426-428.
MITTAL, A.K. e MUNSHI, J.S.D. 1974 On the regeneration and repair of superficial wound in the skin of Rita rita (Ham.) (Bagridae, Pisces).Acta Anat., 88: 424-442.
NOGA, E.J. 1996 Fish Disease. Diagnosis and Treatment. 1a ed. Raleigh: Mosby. 367p.
PETRIC, M.C. 2000 Efeito da suplementação alimentar com vitamina C sobre a formação de gigantócitos em
lamínulas de vidro implantadas no tecido subcutí¢neo de pacus (Piaractus mesopotamicus Holmberg,1887). Jaboticabal-Unesp. 86p. (Dissertação de Mestrado. Faculdade de Ciências Agrárias e Veterinárias, Unesp).
PHROMSUTHIRAK, P. 1977 Electron microscopy of wound healing in the skin of Gasterosteus aculeatus. J. Fish Biol., 11: 193-206.
QUILHAC, A. e SIRE, S.Y. 1999 Spreading,proliferation, and differentiation of the epidermis after wounding a Cichlid fish, Hemichromis bimaculatus. Anat. Rec., 254: 435-451.
RANGANNA, S. 1977 Manual of analysis of fruit and vegetable products. New Delhi: McGraw-Hill.634p.
RYAN, G.B. e SPECTOR, W.G. 1970 Macrophage turnover in inflammed connective tissues. Proc.R. Soc. Lon., ser. B:175: 269-292.
WAHLI, T.; MEIER, W.; PFISTER, K. 1986 Ascorbic acid induced immune mediated decrease in mortality in Ichthyophthirius multifiliis infected rainbow trout (Salmo gairdneri). Acta Trop.,43: 287-289.
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2018-07-02
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