Dietary vitamin C supplementation accelerates the evolution of the healing process in Piaractus mesopotamicus Holmberg, 1887
Keywords:
wound healing, ascorbic acid (vitamin C), Piaractus mesopotamicusAbstract
In this assay, the healing process evolution in juvenile "pacu†Piaractus mesopotamicus Holmberg,1887 (Osteichthyes: Characidae), fed with 0, 100, 200 and 500 mg of ascorbic acid (vitamin C)/kg of ration, was comparatively studied. Rectangular cutaneous hurts (15 mm x 10 mm x 0.25 mm) were done in fish epidermis and derm, and the healing process evolution was studied 1, 3, 7, 14, 21, 28 and 35 days after. The results indicate that the morphological characteristics were different among the treatments, because the ascorbic acid promoted the acceleration of the healing process, through the increasing of the proliferation of mucosal cells, collagen accumulation, tissue remodelation, and scare formation, with a consequently proportional reduction of the wounded area.
References
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ANDERSON, C.D. e ROBERTS, R.J. 1975 A comparision of the effects of temperature on wound healing in a tropical and a temperate teleost. J. Fish Biol., 7: 173-182.
AOAC - Association Official Agriculture Chemists 1975 Official Methods of Analysis. 3. ed.Washington. 975p.
BANZATTO, D. A. e KRONKA, S.N. 1989 Experimentação Agrícola. Jaboticabal: FUNEP.247p.
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BULLOCK, A M.; MARKS, R.; ROBERTS, R.J. 1978a The cell kinetics of teleost fish epidermis: mitotic activity of the normal epidermis at varying temperatures in plaice (Pleuronectes platessa).J. Zool. Lond., 184: 423-428.
BULLOCK, A M.; MARKS, R; ROBERTS, R.J. 1978b The cell kinetics of teleost fish epidermis: epidermal mitotic activity in relation to wound healing at varying temperatures in plaice (Pleuronectes platessa). J. Zool. Lond., 185: 197-204.
CHAVEZ DE MARTINEZ, M.C. e RICHARDS, R.H. 1991 Histopathology of vitamin C deficiency in a cichlid Cichlasoma urophthalmus (Günther). J. Fish Dis.,.14: 507-519.
DURVE, V.S. e LOVELL, R.T. 1982 Vitamin C and disease resistance in channel catfish Ictalurus punctatus. J. Fish. Aquat. Sci., 39: 948-951.
DUTTA, M. e RAI, A.K. 1994 Pattern of cutaneous wound healig in a live fish Clarias batrachus (L) (Clariidae, Pisces). J. Ind. Fish. Assoc., 24: 107-113.
HALVER, J.E. 1953 Fish diseases and nutrition.Trans. Am. Fish. Soc., 83: 254-261.
HALVER, J.E.; SMITH, R.R.; TOLBERT, B.M.; BAKER, L.M. 1975 Utilization of ascorbic acid in fish.Ann. N. Y. Acad. Sci., 258: 81-102.
IGER, Y. e ABRAHAM, M. 1990 The process of skin healing in experimentally wounded carp. J. Fish Biol., 36: 421-437.
IGER, Y.; ABRAHAM, M.; DOTAM, A.; FATTAL, B.; RAHAMIN, E. 1988 Cellular responses in the skin of carp maintained in organically fertilyzed water. J. Fish Biol., 33: 711-720.
INSTITUTO ADOLFO LUTZ 1985 Normas Analíticas: método químico e físico para análise de alimentos. 4 ed. São Paulo. p.345-420.
JAUNCEY, K.; SOLIMAN, A; ROBERTS, J.R. 1985 Ascorbic acid requirements in relation to wound healing in the cultured tilapia Oreochromis niloticus (TREWAVAS). Aquacult. Fish Manage.,16: 139-149.
LI, Y.P. e LOVELL, R.T. 1985 Elevated levels of dietary ascorbic acid increase immune responses in channel catfish. J. Nutr., 115: 123-131.
LOVEL, R.T. 1982 Elevated levels of vitamin C increase disease resistance in channel catfish.Highlights Agric. Res., 29: 124-131.
MARTINS, M.L. 1995 Effect of ascorbic acid deficiency on the growth, gill filament lesions and behavior of pacu fry (Piaractus mesopotamicus, Holmberg, 1887). Braz. J. Med. Biol. Res.,28: 563-568.
MARTINS, M.L. 1998 Evaluation of the addition of ascorbic acid to the ration of cultivated Piaractus mesopotamicus (Characidae) on the infrapopulation of Anacanthorus penilabiatus (Monogenea). Braz. J. Med. Biol. Res.: 31: 655-658.
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MITOMA, C. e SMITH, T.E. 1960 Studies on the role of ascorbic acid in collagen synthesis.J. Biol. Chem., 235: 426-428.
MITTAL, A.K. e MUNSHI, J.S.D. 1974 On the regeneration and repair of superficial wound in the skin of Rita rita (Ham.) (Bagridae, Pisces).Acta Anat., 88: 424-442.
NOGA, E.J. 1996 Fish Disease. Diagnosis and Treatment. 1a ed. Raleigh: Mosby. 367p.
PETRIC, M.C. 2000 Efeito da suplementação alimentar com vitamina C sobre a formação de gigantócitos em
lamínulas de vidro implantadas no tecido subcutí¢neo de pacus (Piaractus mesopotamicus Holmberg,1887). Jaboticabal-Unesp. 86p. (Dissertação de Mestrado. Faculdade de Ciências Agrárias e Veterinárias, Unesp).
PHROMSUTHIRAK, P. 1977 Electron microscopy of wound healing in the skin of Gasterosteus aculeatus. J. Fish Biol., 11: 193-206.
QUILHAC, A. e SIRE, S.Y. 1999 Spreading,proliferation, and differentiation of the epidermis after wounding a Cichlid fish, Hemichromis bimaculatus. Anat. Rec., 254: 435-451.
RANGANNA, S. 1977 Manual of analysis of fruit and vegetable products. New Delhi: McGraw-Hill.634p.
RYAN, G.B. e SPECTOR, W.G. 1970 Macrophage turnover in inflammed connective tissues. Proc.R. Soc. Lon., ser. B:175: 269-292.
WAHLI, T.; MEIER, W.; PFISTER, K. 1986 Ascorbic acid induced immune mediated decrease in mortality in Ichthyophthirius multifiliis infected rainbow trout (Salmo gairdneri). Acta Trop.,43: 287-289.
ANDERSON, C.D. e ROBERTS, R.J. 1975 A comparision of the effects of temperature on wound healing in a tropical and a temperate teleost. J. Fish Biol., 7: 173-182.
AOAC - Association Official Agriculture Chemists 1975 Official Methods of Analysis. 3. ed.Washington. 975p.
BANZATTO, D. A. e KRONKA, S.N. 1989 Experimentação Agrícola. Jaboticabal: FUNEP.247p.
BEREITER-HAHN, J. 1986 Epidermal cell migration and wound repair. In: Biology of the Integument, V.2 -Vertebrates. Heidelberg: Springer.p.443-471.
BULLOCK, A M.; MARKS, R.; ROBERTS, R.J. 1978a The cell kinetics of teleost fish epidermis: mitotic activity of the normal epidermis at varying temperatures in plaice (Pleuronectes platessa).J. Zool. Lond., 184: 423-428.
BULLOCK, A M.; MARKS, R; ROBERTS, R.J. 1978b The cell kinetics of teleost fish epidermis: epidermal mitotic activity in relation to wound healing at varying temperatures in plaice (Pleuronectes platessa). J. Zool. Lond., 185: 197-204.
CHAVEZ DE MARTINEZ, M.C. e RICHARDS, R.H. 1991 Histopathology of vitamin C deficiency in a cichlid Cichlasoma urophthalmus (Günther). J. Fish Dis.,.14: 507-519.
DURVE, V.S. e LOVELL, R.T. 1982 Vitamin C and disease resistance in channel catfish Ictalurus punctatus. J. Fish. Aquat. Sci., 39: 948-951.
DUTTA, M. e RAI, A.K. 1994 Pattern of cutaneous wound healig in a live fish Clarias batrachus (L) (Clariidae, Pisces). J. Ind. Fish. Assoc., 24: 107-113.
HALVER, J.E. 1953 Fish diseases and nutrition.Trans. Am. Fish. Soc., 83: 254-261.
HALVER, J.E.; SMITH, R.R.; TOLBERT, B.M.; BAKER, L.M. 1975 Utilization of ascorbic acid in fish.Ann. N. Y. Acad. Sci., 258: 81-102.
IGER, Y. e ABRAHAM, M. 1990 The process of skin healing in experimentally wounded carp. J. Fish Biol., 36: 421-437.
IGER, Y.; ABRAHAM, M.; DOTAM, A.; FATTAL, B.; RAHAMIN, E. 1988 Cellular responses in the skin of carp maintained in organically fertilyzed water. J. Fish Biol., 33: 711-720.
INSTITUTO ADOLFO LUTZ 1985 Normas Analíticas: método químico e físico para análise de alimentos. 4 ed. São Paulo. p.345-420.
JAUNCEY, K.; SOLIMAN, A; ROBERTS, J.R. 1985 Ascorbic acid requirements in relation to wound healing in the cultured tilapia Oreochromis niloticus (TREWAVAS). Aquacult. Fish Manage.,16: 139-149.
LI, Y.P. e LOVELL, R.T. 1985 Elevated levels of dietary ascorbic acid increase immune responses in channel catfish. J. Nutr., 115: 123-131.
LOVEL, R.T. 1982 Elevated levels of vitamin C increase disease resistance in channel catfish.Highlights Agric. Res., 29: 124-131.
MARTINS, M.L. 1995 Effect of ascorbic acid deficiency on the growth, gill filament lesions and behavior of pacu fry (Piaractus mesopotamicus, Holmberg, 1887). Braz. J. Med. Biol. Res.,28: 563-568.
MARTINS, M.L. 1998 Evaluation of the addition of ascorbic acid to the ration of cultivated Piaractus mesopotamicus (Characidae) on the infrapopulation of Anacanthorus penilabiatus (Monogenea). Braz. J. Med. Biol. Res.: 31: 655-658.
MARTINS, M.L. 2000 Efeito da suplementação com vitamina C sobre a reação inflamatória em Piaractus mesopotamicus Holmberg, 1887 estressados. Unesp - Jaboticabal 125p. (Tese de Doutoramento.Centro de Aqüicultura, Unesp, Jaboticabal, SP).
MITOMA, C. e SMITH, T.E. 1960 Studies on the role of ascorbic acid in collagen synthesis.J. Biol. Chem., 235: 426-428.
MITTAL, A.K. e MUNSHI, J.S.D. 1974 On the regeneration and repair of superficial wound in the skin of Rita rita (Ham.) (Bagridae, Pisces).Acta Anat., 88: 424-442.
NOGA, E.J. 1996 Fish Disease. Diagnosis and Treatment. 1a ed. Raleigh: Mosby. 367p.
PETRIC, M.C. 2000 Efeito da suplementação alimentar com vitamina C sobre a formação de gigantócitos em
lamínulas de vidro implantadas no tecido subcutí¢neo de pacus (Piaractus mesopotamicus Holmberg,1887). Jaboticabal-Unesp. 86p. (Dissertação de Mestrado. Faculdade de Ciências Agrárias e Veterinárias, Unesp).
PHROMSUTHIRAK, P. 1977 Electron microscopy of wound healing in the skin of Gasterosteus aculeatus. J. Fish Biol., 11: 193-206.
QUILHAC, A. e SIRE, S.Y. 1999 Spreading,proliferation, and differentiation of the epidermis after wounding a Cichlid fish, Hemichromis bimaculatus. Anat. Rec., 254: 435-451.
RANGANNA, S. 1977 Manual of analysis of fruit and vegetable products. New Delhi: McGraw-Hill.634p.
RYAN, G.B. e SPECTOR, W.G. 1970 Macrophage turnover in inflammed connective tissues. Proc.R. Soc. Lon., ser. B:175: 269-292.
WAHLI, T.; MEIER, W.; PFISTER, K. 1986 Ascorbic acid induced immune mediated decrease in mortality in Ichthyophthirius multifiliis infected rainbow trout (Salmo gairdneri). Acta Trop.,43: 287-289.
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2018-07-02
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