Chemical composition and evaluation of the antimicrobial activity of two essential oils
DOI:
https://doi.org/10.20950/1678-2305.2018.321Keywords:
Aeromonas spp, citral, linalool, minimum bactericidal concentrationAbstract
This study aimed to identify and quantify the chemical constituents and to evaluate the in vitro antimicrobial activity of essential oils from lemongrass (Cymbopogum flexuosus - EOCF) and basil (Ocimum basilicum - EOOB) against 24 isolates of Aeromonas spp. The main components of EOCF were α-citral (50.13%) and íŸ-citral (40.31%), while those of EOOB were linalool (53.35%) and eucalyptol (11.49%). The EOCF showed high inhibitory activity (â"°¥ 195.31 µg mL-1), whereas EOOB showed moderate inhibitory activity (â"°¥ 781.25 µg mL-1) for Aeromonas spp. Both essential oils have potential for application as antimicrobial agents, in particular EOCF.
References
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Brazil. Brazilian Journal of Microbiology, 35(4): 275-280. http://dx.doi.org/10.1590/S1517-83822004000300001.
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SILVA, F.; FERREIRA, S.; QUEIROZ, J.A.; DOMINGUES, F.C. 2011Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry. Journal of
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AZEVEDO, I.L.; ALMEIDA, A.C.; MARTINS, E.R.; NOGUEIRA, W.C.L.; FARIA FILHO, D.E.; OLIVEIRA, S.P.; PRATES, J.P.B.; SOUZA, C.N. 2016 Eficácia in vitro do óleo essencial de capim-limão (Cymbopogon flexuosus Steud. Wats.) frente a bactérias entéricas de origem avícola. Acta Veterinaria Brasilica, 10(1): 25-31. http://dx.doi.org/10.21708/ avb.2016.10.1.5467.
BAKKALI, F.; AVERBECK, S.; AVERBECK, D.; IDAOMAR, M. 2008 Biological effects of essential oils - a review. Food and Chemical Toxicology, 46(2): 446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106. PMid:17996351.
CASTRO-ESCARPULLI, G.; FIGUERAS, M.J.; AGUILERA-ARREOLA,G.; SOLER, L.; FERNÁNDEZ-RENDÓN, E.; APARICIO, G.O.;UARRO, J.; CHACÓN, M.R. 2003 Characterisation of Aeromonas
spp. isolated from frozen fish intended for human consumption in Mexico. International Journal of Food Microbiology, 84(1): 41-49.http://dx.doi.org/10.1016/S0168-1605(02)00393-8. PMid:12781953.
CLSI í Clinical and Laboratory Standards Institute. 2014 M07-A9: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne: CLSI. 88p.
DEVI, K.P.; NISHA, S.A.; SAKTHIVEL, R.; PANDIAN, S.K. 2010 Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. Journal of
Ethnopharmacology, 130(1): 107-115. http://dx.doi.org/10.1016/j.jep.2010.04.025. PMid:20435121.
GYAWALI, R.; IBRAHIM, S.A. 2014 Natural products as antimicrobial agents. Food Control, 46(1): 412-429. http://dx.doi.org/10.1016/j.foodcont.2014.05.047.
KAKARLA, S.; GANJEWALA, D. 2009 Antimicrobial activity of essential oils of four lemongrass (Cymbopogon flexuosus Steud.) varieties. Medicinal and Aromatic Plant Science and Biotechnology, 3(SI1): 107-109.
LIMMA-NETTO, J.D.; SENA, A.C.; COPATTI, C.E. 2016 Essential oils of Ocimum basilicum and Cymbopogon flexuosus in the seda-tion, anesthesia and recovery of tambacu (Piaractus mesopotamicus male x Colossoma macropomum Female). Boletim do Instituto de Pesca,
42(3): 727-733. http://dx.doi.org/10.20950/1678-2305.2016v42n3p727.
NIST 2010 NIST/EPA/NIH Mass Spectral Library and Search/analysis Programs. Hoboken: J. Wiley and Sons. 49p.
PEEPIM, T.; DONG, H.T.; SENAPIN, S.; KHUNRAE, P.; RATTANAROJPONG, T. 2016 Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia. Aquaculture (Amsterdam, Netherlands), 464(1): 399-409. http://dx.doi.org/10.1016/j.aquaculture.2016.07.022.
PINHEIRO, C.G.; MACHADO, C.M.; AMARAL, L.P.; SILVA, D.T.; ALMEIDA, C.A.A.; LONGHI, S.J.; MALLMANN, C.A.; HEINZMANN, B.M. 2016 Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling. Brazilian Journal of Biology = Revista Brasileira de Biologia, 76(1):
176-184. http://dx.doi.org/10.1590/1519-6984.16314. PMid:26871746.
PINTO, C.P.; RODRIGUES, V.D.; PINTO, F.P.; PINTO, R.P.; UETANABARO,
A.P.T.; PINHEIRO, C.S.R.; GADEA, S.F.M.; SILVA, T.R.S.; LUCCHESE, A.M. 2013 Antimicrobial activity of Lippia species from the Brazilian semiarid region traditionally used as antiseptic and anti-infective agents. Evidence-Based Complementary and Alternative Medicine, 2013(1): 614501. http://dx.doi.org/10.1155/2013/614501.
RADAELLI, M.; SILVA, B.P.; WEIDLICH, L.; HOEHNE, L.; FLACH, A.; COSTA, L.A.; ETHUR, E.M. 2016 Antimicrobial activities of six essential oils commonly used as condiments in Brazil against
Clostridium perfringens. Brazilian Journal of Microbiology, 47(2): 424-430. http://dx.doi.org/10.1016/j.bjm.2015.10.001. PMid:26991289.
SARTORATTO, A.; MACHADO, A.L.M.; DELARMELINA, C.; FIGUEIRA, G.M.; DUARTE, M.C.T.; REHDER, V.L.G. 2004 Composition and antimicrobial activity of essential oils from aromatic plants used in
Brazil. Brazilian Journal of Microbiology, 35(4): 275-280. http://dx.doi.org/10.1590/S1517-83822004000300001.
SIKKEMA, J.; BONT, J.A.M.; POOLMAN, B. 1995 Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews, 59(2):201-222. PMid:7603409.
SILVA, F.; FERREIRA, S.; QUEIROZ, J.A.; DOMINGUES, F.C. 2011Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry. Journal of
Medical Microbiology, 60(10): 1479-1486. http://dx.doi.org/10.1099/jmm.0.034157-0. PMid:21862758.
SNOUSSI, M.; DEHMANI, A.; NOUMI, E.; FLAMINI, G.; PAPETTI, A. 2016 Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. Strains. Microbial Pathogenesis, 90(1): 13-21. http://dx.doi.org/10.1016/j.micpath.2015.11.004. PMid:26596707.
SOUZA, R.C.; COSTA, M.M.; BALDISSEROTTO, B.; HEINZMANN, B.M.; SCHMIDT, D.; CARON, B.O.; COPATTI, C.E. 2017 Antimicrobial and synergistic activity of essential oils of Aloysia triphylla and
Lippia alba against Aeromonas spp. Microbial Pathogenesis, 113(1): 29-33. http://dx.doi.org/10.1016/j.micpath.2017.10.013.PMid:29038058.
YAP, P.S.; YIAP, B.C.; PING, H.C.; LIM, S.H. 2014 Essential oils, a new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal, 8(1): 6-14. http://dx.doi.org/10.2174/18742
85801408010006. PMid:24627729.
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2018-12-21
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