COMPOSIÇÃO QUÍMICA E AVALIAÇÃO DA ATIVIDADE ANTIMICROBIANA DE DOIS ÓLEOS ESSENCIAIS
DOI:
https://doi.org/10.20950/1678-2305.2018.321Palavras-chave:
Aeromonas spp, citral, linalol, concentração bactericida mínimaResumo
Este estudo objetivou identificar e quantificar os constituintes químicos e avaliar a atividade antimicrobiana in vitro dos óleos essenciais de capim limão (Cymbopogum flexuosus - OECF) e manjericão (Ocimum basilicum - OEOB) frente a 24 isolados de Aeromonas spp. Os componentes majoritários de OECF foram o α-citral (50,13%) e íŸ-citral (40,31%), enquanto que de OEOB foram o linalol (53,35%) e eucaliptol (11,49%). OECF demonstrou atividade inibitória elevada (â"°¥ 195,31 µg mL-1), enquanto que OEOB apresentou atividade inibitória moderada (â"°¥ 781,25 µg mL-1) para Aeromonas spp. Ambos os óleos essenciais têm potencial para aplicação como agentes antimicrobianos, principalmente OECF.
Referências
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SARTORATTO, A.; MACHADO, A.L.M.; DELARMELINA, C.; FIGUEIRA, G.M.; DUARTE, M.C.T.; REHDER, V.L.G. 2004 Composition and antimicrobial activity of essential oils from aromatic plants used in
Brazil. Brazilian Journal of Microbiology, 35(4): 275-280. http://dx.doi.org/10.1590/S1517-83822004000300001.
SIKKEMA, J.; BONT, J.A.M.; POOLMAN, B. 1995 Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews, 59(2):201-222. PMid:7603409.
SILVA, F.; FERREIRA, S.; QUEIROZ, J.A.; DOMINGUES, F.C. 2011Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry. Journal of
Medical Microbiology, 60(10): 1479-1486. http://dx.doi.org/10.1099/jmm.0.034157-0. PMid:21862758.
SNOUSSI, M.; DEHMANI, A.; NOUMI, E.; FLAMINI, G.; PAPETTI, A. 2016 Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. Strains. Microbial Pathogenesis, 90(1): 13-21. http://dx.doi.org/10.1016/j.micpath.2015.11.004. PMid:26596707.
SOUZA, R.C.; COSTA, M.M.; BALDISSEROTTO, B.; HEINZMANN, B.M.; SCHMIDT, D.; CARON, B.O.; COPATTI, C.E. 2017 Antimicrobial and synergistic activity of essential oils of Aloysia triphylla and
Lippia alba against Aeromonas spp. Microbial Pathogenesis, 113(1): 29-33. http://dx.doi.org/10.1016/j.micpath.2017.10.013.PMid:29038058.
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AZEVEDO, I.L.; ALMEIDA, A.C.; MARTINS, E.R.; NOGUEIRA, W.C.L.; FARIA FILHO, D.E.; OLIVEIRA, S.P.; PRATES, J.P.B.; SOUZA, C.N. 2016 Eficácia in vitro do óleo essencial de capim-limão (Cymbopogon flexuosus Steud. Wats.) frente a bactérias entéricas de origem avícola. Acta Veterinaria Brasilica, 10(1): 25-31. http://dx.doi.org/10.21708/ avb.2016.10.1.5467.
BAKKALI, F.; AVERBECK, S.; AVERBECK, D.; IDAOMAR, M. 2008 Biological effects of essential oils - a review. Food and Chemical Toxicology, 46(2): 446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106. PMid:17996351.
CASTRO-ESCARPULLI, G.; FIGUERAS, M.J.; AGUILERA-ARREOLA,G.; SOLER, L.; FERNÁNDEZ-RENDÓN, E.; APARICIO, G.O.;UARRO, J.; CHACÓN, M.R. 2003 Characterisation of Aeromonas
spp. isolated from frozen fish intended for human consumption in Mexico. International Journal of Food Microbiology, 84(1): 41-49.http://dx.doi.org/10.1016/S0168-1605(02)00393-8. PMid:12781953.
CLSI í Clinical and Laboratory Standards Institute. 2014 M07-A9: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne: CLSI. 88p.
DEVI, K.P.; NISHA, S.A.; SAKTHIVEL, R.; PANDIAN, S.K. 2010 Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. Journal of
Ethnopharmacology, 130(1): 107-115. http://dx.doi.org/10.1016/j.jep.2010.04.025. PMid:20435121.
GYAWALI, R.; IBRAHIM, S.A. 2014 Natural products as antimicrobial agents. Food Control, 46(1): 412-429. http://dx.doi.org/10.1016/j.foodcont.2014.05.047.
KAKARLA, S.; GANJEWALA, D. 2009 Antimicrobial activity of essential oils of four lemongrass (Cymbopogon flexuosus Steud.) varieties. Medicinal and Aromatic Plant Science and Biotechnology, 3(SI1): 107-109.
LIMMA-NETTO, J.D.; SENA, A.C.; COPATTI, C.E. 2016 Essential oils of Ocimum basilicum and Cymbopogon flexuosus in the seda-tion, anesthesia and recovery of tambacu (Piaractus mesopotamicus male x Colossoma macropomum Female). Boletim do Instituto de Pesca,
42(3): 727-733. http://dx.doi.org/10.20950/1678-2305.2016v42n3p727.
NIST 2010 NIST/EPA/NIH Mass Spectral Library and Search/analysis Programs. Hoboken: J. Wiley and Sons. 49p.
PEEPIM, T.; DONG, H.T.; SENAPIN, S.; KHUNRAE, P.; RATTANAROJPONG, T. 2016 Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia. Aquaculture (Amsterdam, Netherlands), 464(1): 399-409. http://dx.doi.org/10.1016/j.aquaculture.2016.07.022.
PINHEIRO, C.G.; MACHADO, C.M.; AMARAL, L.P.; SILVA, D.T.; ALMEIDA, C.A.A.; LONGHI, S.J.; MALLMANN, C.A.; HEINZMANN, B.M. 2016 Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling. Brazilian Journal of Biology = Revista Brasileira de Biologia, 76(1):
176-184. http://dx.doi.org/10.1590/1519-6984.16314. PMid:26871746.
PINTO, C.P.; RODRIGUES, V.D.; PINTO, F.P.; PINTO, R.P.; UETANABARO,
A.P.T.; PINHEIRO, C.S.R.; GADEA, S.F.M.; SILVA, T.R.S.; LUCCHESE, A.M. 2013 Antimicrobial activity of Lippia species from the Brazilian semiarid region traditionally used as antiseptic and anti-infective agents. Evidence-Based Complementary and Alternative Medicine, 2013(1): 614501. http://dx.doi.org/10.1155/2013/614501.
RADAELLI, M.; SILVA, B.P.; WEIDLICH, L.; HOEHNE, L.; FLACH, A.; COSTA, L.A.; ETHUR, E.M. 2016 Antimicrobial activities of six essential oils commonly used as condiments in Brazil against
Clostridium perfringens. Brazilian Journal of Microbiology, 47(2): 424-430. http://dx.doi.org/10.1016/j.bjm.2015.10.001. PMid:26991289.
SARTORATTO, A.; MACHADO, A.L.M.; DELARMELINA, C.; FIGUEIRA, G.M.; DUARTE, M.C.T.; REHDER, V.L.G. 2004 Composition and antimicrobial activity of essential oils from aromatic plants used in
Brazil. Brazilian Journal of Microbiology, 35(4): 275-280. http://dx.doi.org/10.1590/S1517-83822004000300001.
SIKKEMA, J.; BONT, J.A.M.; POOLMAN, B. 1995 Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews, 59(2):201-222. PMid:7603409.
SILVA, F.; FERREIRA, S.; QUEIROZ, J.A.; DOMINGUES, F.C. 2011Coriander (Coriandrum sativum L.) essential oil: its antibacterial activity and mode of action evaluated by flow cytometry. Journal of
Medical Microbiology, 60(10): 1479-1486. http://dx.doi.org/10.1099/jmm.0.034157-0. PMid:21862758.
SNOUSSI, M.; DEHMANI, A.; NOUMI, E.; FLAMINI, G.; PAPETTI, A. 2016 Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. Strains. Microbial Pathogenesis, 90(1): 13-21. http://dx.doi.org/10.1016/j.micpath.2015.11.004. PMid:26596707.
SOUZA, R.C.; COSTA, M.M.; BALDISSEROTTO, B.; HEINZMANN, B.M.; SCHMIDT, D.; CARON, B.O.; COPATTI, C.E. 2017 Antimicrobial and synergistic activity of essential oils of Aloysia triphylla and
Lippia alba against Aeromonas spp. Microbial Pathogenesis, 113(1): 29-33. http://dx.doi.org/10.1016/j.micpath.2017.10.013.PMid:29038058.
YAP, P.S.; YIAP, B.C.; PING, H.C.; LIM, S.H. 2014 Essential oils, a new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal, 8(1): 6-14. http://dx.doi.org/10.2174/18742
85801408010006. PMid:24627729.
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2018-12-21
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Nota científica (Short Communication)
