Oreochromis niloticus, hematology, super-intensive system, BFT


This trial aimed to evaluate the growth performance and hematological parameters of Nile tilapia (Oreochromis niloticus) GIFT strain during nursery using different stock densities in a biofloc system. The experiment was conducted in circular tanks (400 L) with sexually reversed fish, weighing 6.74 ± 0.37 g, over a period of 35 days. Five treatments with three replicates each were used in a completely randomized design. The treatments were as follows: T1 (200 fishes m-3); T2 (300 fishes m-3); T3 (400 fishes m-3); T4 (500 fishes m-3), and T5 (600 fishes m-3). The fishes were fed four times a day, following a feed table for this species, with adjustments according to fish biomass. The water quality parameters total ammoniacal nitrogen, and unionized ammonia showed a significant difference (p<0.05) between the treatments with lower (T1) and higher stocking densities (T4, T5). Alkalinity was significantly higher in treatments with higher densities (T4 and T5). For hematological parameters, the number of thrombocytes was higher in T5. Hemoglobin concentration was significantly lower in T5 than in T3. The best productive indexes were found in T4, presenting final biomass of 9915.16 ± 14.80 g m-3, apparent feed conversion rate of 1.11 ± 0.02, mean survival of 95.75 ± 0.75%, and daily mean weight gain of 0.43 ± 0.07 g. Overall, the Nile tilapia nursery in a biofloc system showed higher individual growth at densities up to 300 fishes m-3 and increased stocking density up to 500 fishes m-3.


APHA í  American Public Health Association, AWWA í  American Water Works Association, Water Pollution Control Association, 2005. Standard methods for the examination of water and wastewater. 21st ed. Washington: American Public Health Association.

Asche, F.; Roll, K.H.; Tveterí­¥s, S. 2008. Future trends in aquaculture: productivity growth and increased production. In: Holmer, M.; Black, K.; Duarte, C.M.; Marbí­Â , N.; Karakassis, I. Aquaculture in the ecosystem. Dordrecht: Springer. p. 271-292.

Avnimelech, Y. 1999. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture, 176(1-4): 227-235.

Avnimelech, Y. 2006. Bio-filters: the need for a new comprehensive approach. Aquacultural Engineering, 34(3): 172-178.

Avnimelech, Y. 2007. Feeding with microbial flocs by tilapia in minimal discharge bio-flocs technology ponds. Aquaculture, 264(1-4): 140-147.

Avnimelech, Y. 2011. Tilapia production using biofloc technology: saving water, waste recycling improves economics. Global Aquaculture Advocate, 14(3): 66-68.

Avnimelech, Y. 2015. Biofloc technology: a practical guide book. 3rd ed. Baton Rouge: The World Aquaculture Society. 258p.

Avnimelech, Y.; Kochba, M. 2009. Evaluation of nitrogen uptake and excretion by tilapia in bio floc tanks, using 15N tracing. Aquaculture, 287(1-2): 163-168.

Avnimelech, Y.; Kochba, M.; Suryakumar, B.; Ghanekar, B. 2012. Nitrogen isotope: tool to evaluate protein uptake in biofloc systems. Haifa, Israel: Global Aquaculture Alliance. p. 74-75.

Azim, M.E.; Little, D.C. 2008. The biofloc tecnology (BFT) in indoor tanks water quality, bio-floc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture, 283(1-4): 29-35.

Balarin, J.D.; Hatton, J.P. 1979. Tilapia: a guide to their biology and culture in Africa. Scotland: University of Stirling. 174p.

Barbieri, E. 2010. Acute toxicity of ammonia in white shrimp (Litopenaeus schmitti) (Burkenroad, 1936, Crustacea) at different salinity levels. Aquaculture, 306(1-4): 329-333.

Biswas, A.K.; Maita, M.; Yoshizaki, G.; Takeuchi, T. 2004. Physiological responses in Nile tilapia exposed to different photoperiod regimes. Journal of Fish Biology, 65(3): 811-821.

Brum, A.; Pereira, S.A.; Owatari, M.S.; Chagas, E.C.; Chaves, F.C.M.; Mourií­±o, J.L.P.; Martins, M.L. 2017. Effect of dietary essential oils of clove basil and ginger on Nile tilapia (Oreochromis niloticus) following challenge with Streptococcus agalactiae. Aquaculture, 468: 235-243.

Burford, M.A.; Thompson, P.J.; McIntosh, R.P.; Bauman, R.H.; Pearson, D.C. 2003. Nutrient and microbial dynamics in high-intensity, zero-exchange shrimp ponds in Belize. Aquaculture, 219(1-4): 393-411.

Choo, H.X.; Caipang, C.M.A. 2015. Biofloc technology (BFT) and its application towards improved production in freshwater tilapia culture. Aquaculture, Aquarium, Conservation & Legislation, 8(3): 362-366.

Collier, H.B. 1944. Standardization of blood haemoglobin determinations. Canadian Medical Association Journal, 50(6): 550-552.

Correa, A.S.; Pinho, S.M.; Molinari, D.; Pereira, K. da R.; Gutiérrez, S.M.; Monroy-Dosta, M. del C.; Emerenciano, M.G.C. 2020. Rearing of Nile tilapia (Oreochromis niloticus) juveniles in a biofloc system employing periods of feed deprivation. Journal of Applied Aquaculture, 32(2): 139-156.

Crab, R.; Avnimelech, Y.; Defoirdt, T.; Bossier, P.; Verstraete, W. 2007. Nitrogen removal techniques in aquaculture for a sustainable production. Aquaculture, 270(1-4): 1-14.

Daneshvar, E.; Ardestani, M.Y.; Dorafshan, S.; Martins, M.L. 2012. Hematological parameters of Iranian cichlid Iranocichla hormuzensis - Coad, 1982 (Perciformes) in Mehran River. Anais da Academia Brasileira de Ciências, 84(4): 943-949.

Day, S.B.; Salie, K.; Stander, H.B. 2016. A growth comparison among three commercial tilapia species in a biofloc system. Aquaculture International, 24(5): 1309-1322.

De Schryver, P.; Crab, R.; Defoirdt, T.; Boon, N.; Verstraete, W. 2008. The basics of bio-flocs technology: the added value for aquaculture. Aquaculture, 277(3-4): 125-137.

Durigon, E.G.; Lazzari, R.; Uczay, J.; Lopes, D.L. DE A.; Jerônimo, G.T.; Sgnaulin, T.; Emerenciano, M.G.C. 2020. Biofloc technology (BFT): adjusting the levels of digestible protein and digestible energy in diets of Nile tilapia juveniles raised in brackish water. Aquaculture and Fisheries, 5(1): 42-51.

El-Sayed, A.F.M. 2006. Tilapia culture. Wallingford: CAB International. 277p.

El-Shafai, S.A.; El-Gohary, F.A.; Nasr, F.A.; Van der Steen, N.P.; Gijzen, H.J. 2004. Chronic ammonia toxicity to duckweed-fed tilapia (Oreochromis niloticus). Aquaculture, 232(1-4): 117-127.

El-Sherif, M.S.; El-Feky, A.M.I. 2009. Performance of Nile tilapia (Oreochromis niloticus) fingerlings. I. Effect of pH. International Journal of Agriculture and Biology, 11: 297-300.

Emerenciano, M.; Gaxiola, G.; Cuzo, G. 2013. Biofloc technology (BFT): a review for aquaculture application and animal food industry. In: Matovic, M.D. (Ed.). Biomass now-cultivation and utilization. Rijeka: InTech. p. 301-328.

FAO í  Food and Agriculture Organization of the United Nations, 2017. Fisheries and aquaculture statistics 2015. Rome: FAO. 104p.

Gall, G.A.E.; Bakar, Y. 1999. Stocking density and tank size in the design of breed improvement programs for body size of tilapia. Aquaculture, 173(1-4): 197-205.

Ghiraldelli, L.; Martins, M.L.; Yamashita, M.M.; Jeronimo, G.T. 2006. Ectoparasites influence on the haematological parameters of Nile tilapia and carp cultured in the State of Santa Catarina, South Brazil. Su
í­Å“rünleri Dergisi, 1(3): 270-276.

Hargreaves, J.A. 2013. Biofloc production systems for aquaculture. Southern Regional Aquaculture Center, 4503: 1-12.

Hargreaves, J.A.; Kucuk, S. 2001. Effects of diel un-ionised ammonia fluctuation on juvenile hybrid stripped bass, channel catfish and blue tilapia. Aquaculture, 195(1-2): 163-181.

Hari, B.; Madhusoodana Kurup, B.; Varghese, J.T.; Schrama, J.W.; Verdegem, M.C.J. 2004. Effect of carbohydrate addition in extensive shrimp culture systems. Aquaculture, 241(1-4): 179-194.

Ishikawa, N.M.; Ranzani-Paiva, M.J.T.; Lombardi, J.V. 2008. Metodologia para quantificação de leucócitos totais em peixe, Oreochromis niloticus. Archives of Veterinary Science, 13(1): 54-63.

Karasu, B.A.C.; Kí­¶ksal, G. 2005. The acute toxicity of ammonia on tilapia (Oreochromis niloticus) larvae and fingerlings. Turkish Journal of Veterinary and Animal Sciences, 29: 339-344.

Kayode, S.J.; Shamusideen, S.A. 2010. Haematological studies of Oreochromis niloticus exposed to diesel and drilling fluid in Lagos, Nigeria. International Journal of Biodeversity and Conservation, 2(5): 130-133.

Liu, L.; Hu, Z.; Dai, X.; Avnimelech, Y. 2014. Effects of addition of maize starch on the yield, water quality and formation of bioflocs in an integrated shrimp culture system. Aquaculture, 418-419: 79-86.

Long, L.; Yang, J.; Li, Y.; Guan, C.; Wu, F. 2015. Effect of biofloc technology on growth, digestive enzyme activity, hematology, and immune response of genetically improved farmed tilapia (Oreochromis niloticus). Aquaculture, 448: 135-141.

Luo, G.; Gao, Q.; Wang, C.; Liu, W.; Sun, D.; Li, L.; Tan, H. 2014. Growth, digestive activity, welfare, and partial cost-effectiveness of genetically improved farmed tilapia (Oreochromis niloticus) cultured in a recirculating aquaculture system and an indoor biofloc system. Aquaculture, 422-423: 1-7.

Martins, M.L.; Pilarsky, F.; Onaka, E.M.; Nomura, D.T.; Fenerick, J.J.; Ribeiro, K.; Myiazaki, D.M.Y.; Castro, M.P.; Malheiros, E.B. 2004. Haematology and acute inflammatory response of Oreochromis niloticus (Osteichthyes: Cichlidae) submitted to a single and consecutive stress of capture. Boletim do Instituto de Pesca, 30(1): 71-80.

Morgan, J.D.; Sakamoto, T.; Grau, E.G.; Iwama, G.K. 1997. Physiological and respiratory responses of the Mozambique tilapia (Oreochromis mossambicus) to salinity acclimation. Comparative Biochemistry and Physiology. Part A, Physiology, 117(3): 391-398.

Moss, S.M.; Moss, D.R.; Arce, S.M.; Lightner, D.V.; Lotz, J.M. 2012. The role of selective breeding and biosecurity in prevention of disease in penaeid shrimp aquaculture. Journal of Invertebrate Pathology, 110(2): 247-250.

Osman, A.G.M.; AbouelFadl, K.Y.; Abd El Reheem, A.E.B.M.; Mahmoud, U.M.; Kloas, W.; Moustafa, M.A. 2018. Blood biomarkers in Nile tilapia Oreochromis niloticus niloticus and African Catfish Clarias gariepinus to evaluate water quality of the river Nile. Journal of Fisheries Sciences, 12(1): 1-15.

Owatari, M.S.; Jesus, G.F.; Brum, A.; Pereira, S.A.; Lehmann, N.B.; Pereira, U.P.; Martins, M.L.; Mourií­±o, J.L.P. 2018. Sylimarin as hepatic protector and immunomodulator in Nile tilapia during Streptococcus agalactiae infection. Fish & Shellfish Immunology, 82: 565-572.

Owatari, M.S.; Jesus, G.F.; Cardoso, L.; Ferreira, T.H.; Ferrarezi, J.V.S.; Pereira, U.P.; Martins, M.L.; Mourií­±o, J.L.P. 2019. Different via to apply the Gamaxine® commercial biopromoter to Nile tilapia evaluating the immune system responses to Streptococcus agalactiae Ib. Aquaculture, 503: 254-266.

PeixeBR í  Associação Brasileira da Piscicultura, 2019. Anuário 2019. São Paulo. 148p. Available from: <> Access on: 18 Feb, 2019.

Popma, T.J.; Lovshin, L.L. 1996. Worldwide prospects for commercial production of tilapia. Alabama: International Center for Aquaculture and Aquatic Environments. p.42.

Rakocy, J.E. 1989. Tank culture of tilapia. Southern Regional Aquaculture Brasileira de Ciências, 84(4): 943-949. Center, 282: 1-4.

Ranzani-Paiva, M.J.T.; Pádua, S.B.; Tavares-Dias, M.; Egami, M.I. 2013. Métodos para análise hematológica em peixes. Maringá: Eduem. 140p.

Ranzani-Paiva, M.J.T.; Silva-Souza, A.T. 2004. Haematology of Brazilian fish. In: Ranzani-Paiva, M.J.T.; Takemoto, R.M.; Lizama, M.A.P. (Eds.). Sanidade de organismos aquáticos. São Paulo: Varela. p. 89-120.

Ray, A.J.; Seaborn, G.; Leffler, J.W.; Wilde, S.B.; Lawson, A.; Browdy, C.L. 2010. Characterization of microbial communities in minimal exchange, intensive aquaculture systems and the effects of suspended solids management. Aquaculture, 310(1-2): 130-138.

Sá, M.V.C. 2012. Limnocultura: limnologia para aquicultura. Fortaleza: Edições UFC. 218p.

Santacruz-Reyes, R.A.; Chien, Y. 2012. The potential of Yucca schidigera extract to reduce the ammonia pollution from shrimp farming. Bioresource Technology, 113: 311-314.

Santos, V.B.; Mareco, E.A.; Silva, M.D.P. 2013. Growth curves of Nile tilapia (Oreochromis niloticus) strains cultivated at different temperatures. Acta Scientiarum. Animal Sciences, 35(3): 235-242.

Silva, B.C.; Marchiori, N.C. 2018. Importí­¢ncia do manejo alimentar na criação de tilápia. Florianópolis: Epagri. 16p. Available from: <> Access on: 1 Aug, 2018.

Stickney, R.R. 2005. Aquaculture: an introductory text. Cambridge: CABI Publishing. p.265.

Summarwar, S. 2012. Studies on plankton diversity in Bisalpur reservoir. International Journal of Life Sciences Botany and Pharmaceutical Research, 1: 65-72.

Suresh, A.V.; Lin, C.K. 1992. Effect of stocking density on water quality and production of red tilapia in a recirculated water system. Aquacultural Engineering, 11(1): 1-22.

Tamamdusturi, R.; Widanarni.; Yuhana, M. 2016. Administration of microencapsulated probiotic Bacillus sp. NP5 and prebiotic mannan oligosaccharide for prevention of Aeromonas hydrophila infection on Pangasianodon hypophthalmus. Journal of Fisheries and Aquatic Science, 11(1): 67-76.

Tavares-Dias, M.; Martins, M.L.; Kronka, S.N. 1999. Evaluation of the haematological parameters in Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) with Argulus sp. (Crustacea, Branchiura) infestation and treatment with organophosphate. Revista Brasileira de Zoologia, 16(2): 553-555.

Tavares-Dias, M.; Moraes, F. 2007. Leukocyte and thrombocyte reference values for channel catfish (Ictalurus punctatus Raf), with an assessment of morphologic, cytochemical, and ultrastructural features. Veterinary Clinical Pathology, 36(1): 49-54.

Thurston, R.V.; Russo, R.C.; Meyn, E.L.; Zajdel, R.K.; Smith, C.E. 1986. Chronic toxicity of ammonia to fathead minnows. Transactions of the American Fisheries Society, 115(2): 196-207.<196:CTOATF>2.0.CO;2.

Verdegem, M.C.J. 2013. Nutrient discharge from aquaculture operations in function of system design and production environment. Reviews in Aquaculture, 5(3): 158-171.

Vilani, F.G.; Schveitzer, R.; Arantes, R.F.; Vieira, F.N.; Santo, C.M.E.; Seiffert, W.Q. 2016. Strategies for water preparation in a biofloc system: Effects of carbon source and fertilization dose on water quality and shrimp performance. Aquacultural Engineering, 74: 70-75.

Wajsbrot, N.; Gasith, A.; Diamant, A.; Popper, D.M. 1993. Chronic toxicity of ammonia to juvenile gilthead seabream Sparus aurata and related histopathological effects. Journal of Fish Biology, 42(3): 321-328.

Widanarni, A.; Ekasari, J.; Maryam, S. 2012. Evaluation of biofloc technology application on water quality and production performance of red tilapia Oreochromis sp. cultured at different stocking densities. Hayati Journal of Biosciences, 19(2): 73-80.

Wintrobe, M.M. 1934. Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologica. Internationales Magazin fur Blutforschung, 51: 32-49.

Wuertz, S.; Schulze, S.G.E.; Eberhardt, U.; Schulz, C.; Schroeder, J.P. 2013. Acute and chronic nitrite toxicity in juvenile pike-perch (Sander lucioperca) and its compensation by chloride. Comparative Biochemistry and Physiology, 157(4): 352-360.

Xian, J.; Wang, A.; Chen, X.; Gou, N.; Miao, Y.; Liao, S.; Ye, C. 2011. Cytotoxicity of nitrite on haemocytes of the tiger shrimp, Penaeus monodon, using flow cytometric analysis. Aquaculture, 317(1-4): 240-244.

Zaki, M.A.A.; Alabssawy, A.N.; Nour, A.E.-A.M.; El Basuini, M.F.; Dawood, M.A.O.; Alkahtani, S.; Abdel-Daim, M.M. 2020. The impact of stocking density and dietary carbon sources on the growth, oxidative status and stress markers of Nile tilapia (Oreochromis niloticus) reared under biofloc conditions. Aquaculture Reports, 16: 1-8.

Zar, J.H. 2010. Biostatistical analysis. 5th ed. New Jersey: Pretice Hall. 944p.

Zieve, L. 1966. Pathogenesis of hepatic coma. Archives of Internal Medicine, 118(3): 211-223.




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